Amino acid dipepetide frequency for Streptococcus phage Javan399

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.529AlaAla: 0.529 ± 0.256
0.441AlaCys: 0.441 ± 0.194
4.322AlaAsp: 4.322 ± 0.637
3.616AlaGlu: 3.616 ± 0.439
2.47AlaPhe: 2.47 ± 0.396
4.851AlaGly: 4.851 ± 1.009
0.706AlaHis: 0.706 ± 0.319
5.909AlaIle: 5.909 ± 0.785
6.439AlaLys: 6.439 ± 0.916
5.292AlaLeu: 5.292 ± 0.835
2.47AlaMet: 2.47 ± 0.64
4.939AlaAsn: 4.939 ± 0.646
1.852AlaPro: 1.852 ± 0.604
2.999AlaGln: 2.999 ± 0.675
1.94AlaArg: 1.94 ± 0.401
4.498AlaSer: 4.498 ± 0.885
4.234AlaThr: 4.234 ± 0.857
4.851AlaVal: 4.851 ± 0.936
0.706AlaTrp: 0.706 ± 0.303
2.911AlaTyr: 2.911 ± 0.638
0.0AlaXaa: 0.0 ± 0.0
Cys
0.176CysAla: 0.176 ± 0.124
0.0CysCys: 0.0 ± 0.0
0.353CysAsp: 0.353 ± 0.215
0.088CysGlu: 0.088 ± 0.096
0.353CysPhe: 0.353 ± 0.233
0.441CysGly: 0.441 ± 0.264
0.176CysHis: 0.176 ± 0.126
0.353CysIle: 0.353 ± 0.186
0.441CysLys: 0.441 ± 0.28
0.353CysLeu: 0.353 ± 0.165
0.176CysMet: 0.176 ± 0.141
0.353CysAsn: 0.353 ± 0.191
0.176CysPro: 0.176 ± 0.121
0.176CysGln: 0.176 ± 0.141
0.0CysArg: 0.0 ± 0.0
0.176CysSer: 0.176 ± 0.129
0.265CysThr: 0.265 ± 0.144
0.088CysVal: 0.088 ± 0.075
0.088CysTrp: 0.088 ± 0.091
0.441CysTyr: 0.441 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
4.145AspAla: 4.145 ± 0.927
0.353AspCys: 0.353 ± 0.205
4.322AspAsp: 4.322 ± 0.691
5.557AspGlu: 5.557 ± 1.036
3.175AspPhe: 3.175 ± 0.543
5.027AspGly: 5.027 ± 0.59
0.706AspHis: 0.706 ± 0.219
5.821AspIle: 5.821 ± 1.042
4.763AspLys: 4.763 ± 0.569
5.468AspLeu: 5.468 ± 0.831
2.029AspMet: 2.029 ± 0.464
3.704AspAsn: 3.704 ± 0.765
2.029AspPro: 2.029 ± 0.377
1.499AspGln: 1.499 ± 0.381
2.117AspArg: 2.117 ± 0.505
3.793AspSer: 3.793 ± 0.56
3.087AspThr: 3.087 ± 0.554
4.675AspVal: 4.675 ± 0.752
1.058AspTrp: 1.058 ± 0.252
3.704AspTyr: 3.704 ± 0.488
0.0AspXaa: 0.0 ± 0.0
Glu
4.234GluAla: 4.234 ± 0.752
0.265GluCys: 0.265 ± 0.132
3.881GluAsp: 3.881 ± 0.82
3.793GluGlu: 3.793 ± 0.634
2.734GluPhe: 2.734 ± 0.455
2.029GluGly: 2.029 ± 0.438
0.617GluHis: 0.617 ± 0.24
5.468GluIle: 5.468 ± 0.685
6.086GluLys: 6.086 ± 0.723
6.88GluLeu: 6.88 ± 0.888
2.293GluMet: 2.293 ± 0.565
4.586GluAsn: 4.586 ± 0.759
1.411GluPro: 1.411 ± 0.267
3.087GluGln: 3.087 ± 0.601
3.087GluArg: 3.087 ± 0.797
3.793GluSer: 3.793 ± 0.548
4.057GluThr: 4.057 ± 0.619
3.881GluVal: 3.881 ± 0.517
1.058GluTrp: 1.058 ± 0.349
2.117GluTyr: 2.117 ± 0.509
0.0GluXaa: 0.0 ± 0.0
Phe
2.646PheAla: 2.646 ± 0.601
0.176PheCys: 0.176 ± 0.109
3.881PheAsp: 3.881 ± 0.616
2.822PheGlu: 2.822 ± 0.467
1.94PhePhe: 1.94 ± 0.37
3.352PheGly: 3.352 ± 0.555
0.706PheHis: 0.706 ± 0.29
2.205PheIle: 2.205 ± 0.507
3.528PheLys: 3.528 ± 0.649
3.352PheLeu: 3.352 ± 0.571
0.617PheMet: 0.617 ± 0.292
2.822PheAsn: 2.822 ± 0.493
0.617PhePro: 0.617 ± 0.192
1.323PheGln: 1.323 ± 0.344
1.676PheArg: 1.676 ± 0.379
2.381PheSer: 2.381 ± 0.443
2.029PheThr: 2.029 ± 0.592
2.558PheVal: 2.558 ± 0.59
0.353PheTrp: 0.353 ± 0.165
1.764PheTyr: 1.764 ± 0.469
0.0PheXaa: 0.0 ± 0.0
Gly
3.616GlyAla: 3.616 ± 1.014
0.353GlyCys: 0.353 ± 0.223
3.44GlyAsp: 3.44 ± 0.548
3.352GlyGlu: 3.352 ± 0.413
2.911GlyPhe: 2.911 ± 0.575
4.234GlyGly: 4.234 ± 0.946
1.323GlyHis: 1.323 ± 0.332
5.821GlyIle: 5.821 ± 0.766
5.733GlyLys: 5.733 ± 0.664
5.645GlyLeu: 5.645 ± 0.736
2.646GlyMet: 2.646 ± 0.58
3.616GlyAsn: 3.616 ± 1.045
0.441GlyPro: 0.441 ± 0.194
2.558GlyGln: 2.558 ± 0.617
2.381GlyArg: 2.381 ± 0.489
4.675GlySer: 4.675 ± 0.799
4.145GlyThr: 4.145 ± 0.736
4.057GlyVal: 4.057 ± 0.701
1.411GlyTrp: 1.411 ± 0.345
3.528GlyTyr: 3.528 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.058HisAla: 1.058 ± 0.398
0.176HisCys: 0.176 ± 0.133
1.235HisAsp: 1.235 ± 0.348
1.058HisGlu: 1.058 ± 0.288
0.617HisPhe: 0.617 ± 0.251
0.97HisGly: 0.97 ± 0.382
0.088HisHis: 0.088 ± 0.089
1.323HisIle: 1.323 ± 0.299
1.411HisLys: 1.411 ± 0.377
0.97HisLeu: 0.97 ± 0.356
0.353HisMet: 0.353 ± 0.155
0.794HisAsn: 0.794 ± 0.22
0.441HisPro: 0.441 ± 0.205
0.617HisGln: 0.617 ± 0.22
0.529HisArg: 0.529 ± 0.245
0.882HisSer: 0.882 ± 0.336
0.794HisThr: 0.794 ± 0.22
0.97HisVal: 0.97 ± 0.258
0.176HisTrp: 0.176 ± 0.133
0.617HisTyr: 0.617 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
5.204IleAla: 5.204 ± 0.735
0.529IleCys: 0.529 ± 0.237
4.851IleAsp: 4.851 ± 0.7
5.557IleGlu: 5.557 ± 0.749
2.47IlePhe: 2.47 ± 0.63
4.763IleGly: 4.763 ± 0.557
1.323IleHis: 1.323 ± 0.294
3.263IleIle: 3.263 ± 0.549
6.703IleLys: 6.703 ± 0.801
5.204IleLeu: 5.204 ± 0.669
1.499IleMet: 1.499 ± 0.37
4.498IleAsn: 4.498 ± 0.563
2.734IlePro: 2.734 ± 0.649
2.911IleGln: 2.911 ± 0.493
1.94IleArg: 1.94 ± 0.435
5.116IleSer: 5.116 ± 0.575
5.027IleThr: 5.027 ± 0.765
4.322IleVal: 4.322 ± 0.763
0.97IleTrp: 0.97 ± 0.294
1.94IleTyr: 1.94 ± 0.543
0.0IleXaa: 0.0 ± 0.0
Lys
6.262LysAla: 6.262 ± 0.97
0.176LysCys: 0.176 ± 0.148
5.38LysAsp: 5.38 ± 0.678
5.733LysGlu: 5.733 ± 0.623
3.352LysPhe: 3.352 ± 0.74
4.851LysGly: 4.851 ± 0.599
1.588LysHis: 1.588 ± 0.425
6.88LysIle: 6.88 ± 0.867
7.232LysLys: 7.232 ± 0.85
6.439LysLeu: 6.439 ± 0.782
1.588LysMet: 1.588 ± 0.396
4.234LysAsn: 4.234 ± 0.546
1.94LysPro: 1.94 ± 0.389
3.793LysGln: 3.793 ± 0.581
2.911LysArg: 2.911 ± 0.49
5.998LysSer: 5.998 ± 0.943
5.733LysThr: 5.733 ± 0.793
6.35LysVal: 6.35 ± 0.86
1.235LysTrp: 1.235 ± 0.31
3.263LysTyr: 3.263 ± 0.568
0.0LysXaa: 0.0 ± 0.0
Leu
6.968LeuAla: 6.968 ± 1.085
0.088LeuCys: 0.088 ± 0.091
6.086LeuAsp: 6.086 ± 0.822
4.851LeuGlu: 4.851 ± 0.744
2.734LeuPhe: 2.734 ± 0.443
4.851LeuGly: 4.851 ± 0.661
1.588LeuHis: 1.588 ± 0.498
4.41LeuIle: 4.41 ± 0.601
7.321LeuLys: 7.321 ± 0.715
6.527LeuLeu: 6.527 ± 0.868
1.94LeuMet: 1.94 ± 0.443
6.791LeuAsn: 6.791 ± 0.599
2.558LeuPro: 2.558 ± 0.497
3.704LeuGln: 3.704 ± 0.65
3.087LeuArg: 3.087 ± 0.684
5.645LeuSer: 5.645 ± 0.69
5.821LeuThr: 5.821 ± 0.977
4.41LeuVal: 4.41 ± 0.597
0.882LeuTrp: 0.882 ± 0.24
2.029LeuTyr: 2.029 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
2.117MetAla: 2.117 ± 0.5
0.088MetCys: 0.088 ± 0.089
0.794MetAsp: 0.794 ± 0.297
1.323MetGlu: 1.323 ± 0.359
0.97MetPhe: 0.97 ± 0.242
1.235MetGly: 1.235 ± 0.362
0.441MetHis: 0.441 ± 0.253
1.852MetIle: 1.852 ± 0.45
2.47MetLys: 2.47 ± 0.496
1.94MetLeu: 1.94 ± 0.499
0.529MetMet: 0.529 ± 0.234
2.205MetAsn: 2.205 ± 0.448
0.706MetPro: 0.706 ± 0.208
0.794MetGln: 0.794 ± 0.248
0.882MetArg: 0.882 ± 0.231
1.94MetSer: 1.94 ± 0.415
2.381MetThr: 2.381 ± 0.487
1.676MetVal: 1.676 ± 0.409
0.176MetTrp: 0.176 ± 0.151
0.882MetTyr: 0.882 ± 0.263
0.0MetXaa: 0.0 ± 0.0
Asn
5.292AsnAla: 5.292 ± 0.99
0.265AsnCys: 0.265 ± 0.14
3.881AsnAsp: 3.881 ± 0.722
3.352AsnGlu: 3.352 ± 0.554
2.293AsnPhe: 2.293 ± 0.434
7.144AsnGly: 7.144 ± 1.051
1.235AsnHis: 1.235 ± 0.376
4.234AsnIle: 4.234 ± 0.601
5.645AsnLys: 5.645 ± 0.593
4.498AsnLeu: 4.498 ± 0.541
1.852AsnMet: 1.852 ± 0.367
5.733AsnAsn: 5.733 ± 0.83
2.999AsnPro: 2.999 ± 0.434
2.734AsnGln: 2.734 ± 0.544
2.117AsnArg: 2.117 ± 0.409
3.44AsnSer: 3.44 ± 0.598
3.704AsnThr: 3.704 ± 0.804
2.646AsnVal: 2.646 ± 0.466
1.235AsnTrp: 1.235 ± 0.339
2.734AsnTyr: 2.734 ± 0.582
0.0AsnXaa: 0.0 ± 0.0
Pro
1.411ProAla: 1.411 ± 0.334
0.088ProCys: 0.088 ± 0.099
1.499ProAsp: 1.499 ± 0.424
1.94ProGlu: 1.94 ± 0.425
1.147ProPhe: 1.147 ± 0.351
0.706ProGly: 0.706 ± 0.285
0.441ProHis: 0.441 ± 0.165
1.94ProIle: 1.94 ± 0.447
2.47ProLys: 2.47 ± 0.463
2.117ProLeu: 2.117 ± 0.447
0.706ProMet: 0.706 ± 0.254
2.117ProAsn: 2.117 ± 0.372
0.617ProPro: 0.617 ± 0.283
1.323ProGln: 1.323 ± 0.25
1.058ProArg: 1.058 ± 0.377
2.117ProSer: 2.117 ± 0.466
2.293ProThr: 2.293 ± 0.406
1.588ProVal: 1.588 ± 0.373
0.353ProTrp: 0.353 ± 0.164
0.97ProTyr: 0.97 ± 0.342
0.0ProXaa: 0.0 ± 0.0
Gln
3.969GlnAla: 3.969 ± 0.626
0.088GlnCys: 0.088 ± 0.096
2.293GlnAsp: 2.293 ± 0.515
2.205GlnGlu: 2.205 ± 0.362
2.117GlnPhe: 2.117 ± 0.434
2.293GlnGly: 2.293 ± 0.4
0.794GlnHis: 0.794 ± 0.33
2.381GlnIle: 2.381 ± 0.638
3.263GlnLys: 3.263 ± 0.416
4.145GlnLeu: 4.145 ± 0.613
1.235GlnMet: 1.235 ± 0.301
2.117GlnAsn: 2.117 ± 0.371
1.058GlnPro: 1.058 ± 0.36
2.47GlnGln: 2.47 ± 0.408
1.764GlnArg: 1.764 ± 0.402
2.381GlnSer: 2.381 ± 0.483
2.47GlnThr: 2.47 ± 0.412
2.381GlnVal: 2.381 ± 0.415
0.882GlnTrp: 0.882 ± 0.316
1.411GlnTyr: 1.411 ± 0.32
0.0GlnXaa: 0.0 ± 0.0
Arg
1.852ArgAla: 1.852 ± 0.337
0.176ArgCys: 0.176 ± 0.116
2.381ArgAsp: 2.381 ± 0.564
2.646ArgGlu: 2.646 ± 0.515
1.058ArgPhe: 1.058 ± 0.306
2.117ArgGly: 2.117 ± 0.368
0.706ArgHis: 0.706 ± 0.233
2.646ArgIle: 2.646 ± 0.56
3.352ArgLys: 3.352 ± 0.666
3.352ArgLeu: 3.352 ± 0.635
0.794ArgMet: 0.794 ± 0.3
3.087ArgAsn: 3.087 ± 0.534
0.441ArgPro: 0.441 ± 0.224
1.94ArgGln: 1.94 ± 0.41
1.764ArgArg: 1.764 ± 0.443
1.588ArgSer: 1.588 ± 0.423
1.852ArgThr: 1.852 ± 0.594
1.499ArgVal: 1.499 ± 0.346
0.529ArgTrp: 0.529 ± 0.203
1.94ArgTyr: 1.94 ± 0.412
0.0ArgXaa: 0.0 ± 0.0
Ser
4.145SerAla: 4.145 ± 0.621
0.265SerCys: 0.265 ± 0.154
4.851SerAsp: 4.851 ± 0.857
5.027SerGlu: 5.027 ± 0.65
3.616SerPhe: 3.616 ± 0.649
5.204SerGly: 5.204 ± 0.771
0.441SerHis: 0.441 ± 0.199
4.145SerIle: 4.145 ± 0.439
5.733SerLys: 5.733 ± 0.969
5.027SerLeu: 5.027 ± 0.659
1.411SerMet: 1.411 ± 0.387
4.498SerAsn: 4.498 ± 0.693
1.764SerPro: 1.764 ± 0.531
1.852SerGln: 1.852 ± 0.363
2.205SerArg: 2.205 ± 0.561
5.204SerSer: 5.204 ± 1.462
3.352SerThr: 3.352 ± 0.515
3.616SerVal: 3.616 ± 0.537
0.882SerTrp: 0.882 ± 0.393
2.205SerTyr: 2.205 ± 0.43
0.0SerXaa: 0.0 ± 0.0
Thr
5.733ThrAla: 5.733 ± 0.899
0.088ThrCys: 0.088 ± 0.083
4.145ThrAsp: 4.145 ± 0.611
4.057ThrGlu: 4.057 ± 0.48
2.205ThrPhe: 2.205 ± 0.469
5.292ThrGly: 5.292 ± 0.9
0.617ThrHis: 0.617 ± 0.24
4.851ThrIle: 4.851 ± 0.848
4.145ThrLys: 4.145 ± 0.699
5.116ThrLeu: 5.116 ± 0.684
1.147ThrMet: 1.147 ± 0.28
3.881ThrAsn: 3.881 ± 0.646
1.588ThrPro: 1.588 ± 0.41
2.999ThrGln: 2.999 ± 0.497
1.676ThrArg: 1.676 ± 0.38
4.234ThrSer: 4.234 ± 0.939
4.41ThrThr: 4.41 ± 0.885
3.616ThrVal: 3.616 ± 0.657
0.882ThrTrp: 0.882 ± 0.318
2.293ThrTyr: 2.293 ± 0.571
0.0ThrXaa: 0.0 ± 0.0
Val
3.969ValAla: 3.969 ± 0.502
0.176ValCys: 0.176 ± 0.144
4.763ValAsp: 4.763 ± 0.718
4.057ValGlu: 4.057 ± 0.669
2.117ValPhe: 2.117 ± 0.359
3.969ValGly: 3.969 ± 0.68
0.529ValHis: 0.529 ± 0.225
4.498ValIle: 4.498 ± 0.715
4.763ValLys: 4.763 ± 0.674
4.763ValLeu: 4.763 ± 0.503
1.235ValMet: 1.235 ± 0.335
4.322ValAsn: 4.322 ± 0.508
1.94ValPro: 1.94 ± 0.494
1.764ValGln: 1.764 ± 0.334
2.381ValArg: 2.381 ± 0.441
4.234ValSer: 4.234 ± 0.649
3.616ValThr: 3.616 ± 0.753
3.616ValVal: 3.616 ± 0.691
0.441ValTrp: 0.441 ± 0.157
2.117ValTyr: 2.117 ± 0.37
0.0ValXaa: 0.0 ± 0.0
Trp
0.706TrpAla: 0.706 ± 0.287
0.176TrpCys: 0.176 ± 0.143
1.147TrpAsp: 1.147 ± 0.298
1.147TrpGlu: 1.147 ± 0.361
0.176TrpPhe: 0.176 ± 0.122
0.617TrpGly: 0.617 ± 0.207
0.353TrpHis: 0.353 ± 0.176
0.529TrpIle: 0.529 ± 0.187
1.235TrpLys: 1.235 ± 0.401
1.323TrpLeu: 1.323 ± 0.398
0.176TrpMet: 0.176 ± 0.103
0.794TrpAsn: 0.794 ± 0.331
0.353TrpPro: 0.353 ± 0.205
0.97TrpGln: 0.97 ± 0.377
0.617TrpArg: 0.617 ± 0.231
0.706TrpSer: 0.706 ± 0.326
1.323TrpThr: 1.323 ± 0.284
0.706TrpVal: 0.706 ± 0.202
0.176TrpTrp: 0.176 ± 0.105
0.529TrpTyr: 0.529 ± 0.321
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.94TyrAla: 1.94 ± 0.424
0.706TyrCys: 0.706 ± 0.224
3.528TyrAsp: 3.528 ± 0.687
3.263TyrGlu: 3.263 ± 0.509
2.293TyrPhe: 2.293 ± 0.396
1.94TyrGly: 1.94 ± 0.431
0.706TyrHis: 0.706 ± 0.257
2.293TyrIle: 2.293 ± 0.496
2.205TyrLys: 2.205 ± 0.454
3.881TyrLeu: 3.881 ± 0.544
0.617TyrMet: 0.617 ± 0.289
1.94TyrAsn: 1.94 ± 0.468
1.235TyrPro: 1.235 ± 0.297
2.205TyrGln: 2.205 ± 0.48
1.588TyrArg: 1.588 ± 0.454
2.646TyrSer: 2.646 ± 0.466
2.381TyrThr: 2.381 ± 0.624
1.764TyrVal: 1.764 ± 0.466
0.265TyrTrp: 0.265 ± 0.132
1.588TyrTyr: 1.588 ± 0.417
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11339 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski