Nitrospirillum amazonense Y2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Nitrospirillum; Nitrospirillum amazonense

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1Y423|G1Y423_9PROT Uncharacterized protein OS=Nitrospirillum amazonense Y2 OX=1003237 GN=AZA_26535 PE=4 SV=1
MM1 pKa = 7.06NVAAVGDD8 pKa = 4.21YY9 pKa = 11.03NAITATGTGSGIFVGGTGDD28 pKa = 3.98TITASSSSIYY38 pKa = 10.29LYY40 pKa = 11.29SNTQTAANLLTVNGTSNTISLVANWNVTLTLSGNAITATGGTITLSTILTEE91 pKa = 4.18TLTGSGSTVTMASGGTLYY109 pKa = 10.5INGGGQFADD118 pKa = 3.72ANAVTLSNGTLILDD132 pKa = 3.79HH133 pKa = 7.32DD134 pKa = 4.29ARR136 pKa = 11.84ANLTGSGNHH145 pKa = 4.98VTVGYY150 pKa = 9.86NDD152 pKa = 3.55NVGVTGDD159 pKa = 3.98NNILTATGSGDD170 pKa = 3.85GVWVGGTNNAVTISNGSVYY189 pKa = 10.81VGGLQTQITGNNDD202 pKa = 3.73AITLYY207 pKa = 10.75GGIGATINGNNEE219 pKa = 4.15VISVNGPNTTVALSTNGVGPSGEE242 pKa = 4.23LDD244 pKa = 3.4LFVTHH249 pKa = 6.76DD250 pKa = 3.78QVWLQKK256 pKa = 10.54SGNDD260 pKa = 4.21LVVDD264 pKa = 4.66LNNGTTEE271 pKa = 4.07TTLTNWFLTGGTGTYY286 pKa = 10.25DD287 pKa = 3.23HH288 pKa = 6.53QVANIKK294 pKa = 9.73TSDD297 pKa = 3.54GYY299 pKa = 11.09FLSSSSIDD307 pKa = 3.52TLLSGSNTTASSSVLSGYY325 pKa = 7.77WHH327 pKa = 6.42TT328 pKa = 4.55

Molecular weight: 32.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1XZD6|G1XZD6_9PROT Chemotaxis MotB protein OS=Nitrospirillum amazonense Y2 OX=1003237 GN=AZA_88725 PE=4 SV=1
MM1 pKa = 7.48AAMMASVSGRR11 pKa = 11.84GAGPPMRR18 pKa = 11.84AVSPGIRR25 pKa = 11.84VTRR28 pKa = 11.84APALSGMVMAQRR40 pKa = 11.84VSSNSAMGRR49 pKa = 11.84RR50 pKa = 11.84LRR52 pKa = 4.08

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3357

0

3357

1202703

42

3041

358.3

38.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.606 ± 0.065

0.752 ± 0.011

6.014 ± 0.036

4.757 ± 0.041

3.357 ± 0.028

9.357 ± 0.058

2.287 ± 0.026

4.344 ± 0.035

2.854 ± 0.032

9.978 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.019

2.704 ± 0.033

5.453 ± 0.036

3.788 ± 0.033

7.087 ± 0.05

5.072 ± 0.046

5.802 ± 0.046

7.664 ± 0.035

1.373 ± 0.018

2.513 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski