Amino acid dipepetide frequency for Nitrospirillum amazonense Y2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.781AlaAla: 17.781 ± 0.229	1.016AlaCys: 1.016 ± 0.032	7.727AlaAsp: 7.727 ± 0.121	6.654AlaGlu: 6.654 ± 0.108	3.939AlaPhe: 3.939 ± 0.057	12.385AlaGly: 12.385 ± 0.193	2.644AlaHis: 2.644 ± 0.056	5.208AlaIle: 5.208 ± 0.072	3.866AlaLys: 3.866 ± 0.071	12.957AlaLeu: 12.957 ± 0.134	2.992AlaMet: 2.992 ± 0.059	3.119AlaAsn: 3.119 ± 0.065	5.905AlaPro: 5.905 ± 0.077	4.945AlaGln: 4.945 ± 0.086	8.292AlaArg: 8.292 ± 0.115	5.853AlaSer: 5.853 ± 0.085	6.728AlaThr: 6.728 ± 0.1	9.494AlaVal: 9.494 ± 0.129	1.68AlaTrp: 1.68 ± 0.039	2.875AlaTyr: 2.875 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.858CysAla: 0.858 ± 0.024	0.105CysCys: 0.105 ± 0.009	0.422CysAsp: 0.422 ± 0.021	0.298CysGlu: 0.298 ± 0.017	0.272CysPhe: 0.272 ± 0.016	0.78CysGly: 0.78 ± 0.029	0.22CysHis: 0.22 ± 0.016	0.307CysIle: 0.307 ± 0.018	0.144CysLys: 0.144 ± 0.012	0.785CysLeu: 0.785 ± 0.029	0.126CysMet: 0.126 ± 0.01	0.176CysAsn: 0.176 ± 0.012	0.506CysPro: 0.506 ± 0.019	0.257CysGln: 0.257 ± 0.015	0.663CysArg: 0.663 ± 0.027	0.371CysSer: 0.371 ± 0.019	0.414CysThr: 0.414 ± 0.02	0.503CysVal: 0.503 ± 0.021	0.121CysTrp: 0.121 ± 0.01	0.195CysTyr: 0.195 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.235AspAla: 7.235 ± 0.099	0.403AspCys: 0.403 ± 0.017	3.333AspAsp: 3.333 ± 0.06	2.883AspGlu: 2.883 ± 0.055	2.095AspPhe: 2.095 ± 0.047	6.436AspGly: 6.436 ± 0.125	1.463AspHis: 1.463 ± 0.043	2.952AspIle: 2.952 ± 0.061	1.591AspLys: 1.591 ± 0.043	6.446AspLeu: 6.446 ± 0.085	1.307AspMet: 1.307 ± 0.034	1.408AspAsn: 1.408 ± 0.037	3.322AspPro: 3.322 ± 0.05	2.37AspGln: 2.37 ± 0.048	4.475AspArg: 4.475 ± 0.073	2.338AspSer: 2.338 ± 0.052	2.995AspThr: 2.995 ± 0.052	4.273AspVal: 4.273 ± 0.07	1.057AspTrp: 1.057 ± 0.033	1.759AspTyr: 1.759 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.401GluAla: 6.401 ± 0.088	0.255GluCys: 0.255 ± 0.016	2.462GluAsp: 2.462 ± 0.05	2.651GluGlu: 2.651 ± 0.057	1.489GluPhe: 1.489 ± 0.037	4.186GluGly: 4.186 ± 0.073	1.052GluHis: 1.052 ± 0.03	2.513GluIle: 2.513 ± 0.055	1.725GluLys: 1.725 ± 0.047	4.349GluLeu: 4.349 ± 0.078	1.248GluMet: 1.248 ± 0.032	1.278GluAsn: 1.278 ± 0.033	2.104GluPro: 2.104 ± 0.043	1.909GluGln: 1.909 ± 0.044	3.957GluArg: 3.957 ± 0.071	1.877GluSer: 1.877 ± 0.04	2.879GluThr: 2.879 ± 0.056	3.689GluVal: 3.689 ± 0.058	0.56GluTrp: 0.56 ± 0.023	0.989GluTyr: 0.989 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.505PheAla: 3.505 ± 0.062	0.312PheCys: 0.312 ± 0.015	2.377PheAsp: 2.377 ± 0.051	1.553PheGlu: 1.553 ± 0.041	1.248PhePhe: 1.248 ± 0.039	3.344PheGly: 3.344 ± 0.051	0.775PheHis: 0.775 ± 0.023	1.505PheIle: 1.505 ± 0.04	0.996PheLys: 0.996 ± 0.027	3.176PheLeu: 3.176 ± 0.057	0.774PheMet: 0.774 ± 0.029	1.187PheAsn: 1.187 ± 0.037	1.623PhePro: 1.623 ± 0.038	1.225PheGln: 1.225 ± 0.032	2.085PheArg: 2.085 ± 0.05	2.027PheSer: 2.027 ± 0.048	2.151PheThr: 2.151 ± 0.056	2.262PheVal: 2.262 ± 0.041	0.467PheTrp: 0.467 ± 0.022	0.977PheTyr: 0.977 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	10.451GlyAla: 10.451 ± 0.186	0.782GlyCys: 0.782 ± 0.03	5.445GlyAsp: 5.445 ± 0.099	4.149GlyGlu: 4.149 ± 0.069	3.461GlyPhe: 3.461 ± 0.057	9.364GlyGly: 9.364 ± 0.177	2.432GlyHis: 2.432 ± 0.061	4.245GlyIle: 4.245 ± 0.073	2.928GlyLys: 2.928 ± 0.058	8.98GlyLeu: 8.98 ± 0.092	2.142GlyMet: 2.142 ± 0.042	2.457GlyAsn: 2.457 ± 0.068	4.093GlyPro: 4.093 ± 0.068	4.123GlyGln: 4.123 ± 0.11	6.936GlyArg: 6.936 ± 0.096	4.484GlySer: 4.484 ± 0.09	5.651GlyThr: 5.651 ± 0.157	7.318GlyVal: 7.318 ± 0.102	1.494GlyTrp: 1.494 ± 0.04	2.637GlyTyr: 2.637 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.549HisAla: 2.549 ± 0.055	0.21HisCys: 0.21 ± 0.013	1.37HisAsp: 1.37 ± 0.037	0.997HisGlu: 0.997 ± 0.03	0.821HisPhe: 0.821 ± 0.026	2.323HisGly: 2.323 ± 0.056	0.856HisHis: 0.856 ± 0.037	0.949HisIle: 0.949 ± 0.03	0.464HisLys: 0.464 ± 0.017	2.392HisLeu: 2.392 ± 0.053	0.457HisMet: 0.457 ± 0.023	0.481HisAsn: 0.481 ± 0.02	1.511HisPro: 1.511 ± 0.04	1.067HisGln: 1.067 ± 0.049	1.879HisArg: 1.879 ± 0.061	0.878HisSer: 0.878 ± 0.027	1.034HisThr: 1.034 ± 0.029	1.638HisVal: 1.638 ± 0.042	0.353HisTrp: 0.353 ± 0.019	0.642HisTyr: 0.642 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.534IleAla: 5.534 ± 0.075	0.368IleCys: 0.368 ± 0.019	3.086IleAsp: 3.086 ± 0.05	2.395IleGlu: 2.395 ± 0.054	1.223IlePhe: 1.223 ± 0.036	4.269IleGly: 4.269 ± 0.083	0.911IleHis: 0.911 ± 0.028	1.791IleIle: 1.791 ± 0.048	1.158IleLys: 1.158 ± 0.037	3.782IleLeu: 3.782 ± 0.063	0.794IleMet: 0.794 ± 0.03	1.369IleAsn: 1.369 ± 0.043	2.242IlePro: 2.242 ± 0.035	1.52IleGln: 1.52 ± 0.035	2.81IleArg: 2.81 ± 0.051	2.65IleSer: 2.65 ± 0.054	2.816IleThr: 2.816 ± 0.059	3.262IleVal: 3.262 ± 0.052	0.456IleTrp: 0.456 ± 0.02	1.0IleTyr: 1.0 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.166LysAla: 4.166 ± 0.076	0.118LysCys: 0.118 ± 0.009	1.644LysAsp: 1.644 ± 0.046	1.312LysGlu: 1.312 ± 0.042	0.773LysPhe: 0.773 ± 0.026	2.578LysGly: 2.578 ± 0.05	0.453LysHis: 0.453 ± 0.02	1.298LysIle: 1.298 ± 0.035	0.938LysLys: 0.938 ± 0.034	2.753LysLeu: 2.753 ± 0.057	0.641LysMet: 0.641 ± 0.025	0.723LysAsn: 0.723 ± 0.028	1.862LysPro: 1.862 ± 0.047	0.858LysGln: 0.858 ± 0.028	1.937LysArg: 1.937 ± 0.041	1.473LysSer: 1.473 ± 0.039	1.655LysThr: 1.655 ± 0.041	2.39LysVal: 2.39 ± 0.056	0.316LysTrp: 0.316 ± 0.017	0.656LysTyr: 0.656 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	13.528LeuAla: 13.528 ± 0.138	0.772LeuCys: 0.772 ± 0.03	6.258LeuAsp: 6.258 ± 0.075	4.668LeuGlu: 4.668 ± 0.076	3.396LeuPhe: 3.396 ± 0.068	8.336LeuGly: 8.336 ± 0.104	2.079LeuHis: 2.079 ± 0.054	3.994LeuIle: 3.994 ± 0.068	3.283LeuLys: 3.283 ± 0.064	9.201LeuLeu: 9.201 ± 0.134	2.264LeuMet: 2.264 ± 0.048	2.816LeuAsn: 2.816 ± 0.061	5.837LeuPro: 5.837 ± 0.082	2.942LeuGln: 2.942 ± 0.056	7.012LeuArg: 7.012 ± 0.09	6.18LeuSer: 6.18 ± 0.09	6.191LeuThr: 6.191 ± 0.091	7.598LeuVal: 7.598 ± 0.103	1.168LeuTrp: 1.168 ± 0.035	2.262LeuTyr: 2.262 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	3.304MetAla: 3.304 ± 0.061	0.121MetCys: 0.121 ± 0.011	1.218MetAsp: 1.218 ± 0.035	1.079MetGlu: 1.079 ± 0.028	0.545MetPhe: 0.545 ± 0.024	1.971MetGly: 1.971 ± 0.043	0.386MetHis: 0.386 ± 0.018	0.925MetIle: 0.925 ± 0.03	0.713MetLys: 0.713 ± 0.023	2.012MetLeu: 2.012 ± 0.046	0.599MetMet: 0.599 ± 0.024	0.607MetAsn: 0.607 ± 0.023	1.435MetPro: 1.435 ± 0.035	0.678MetGln: 0.678 ± 0.024	1.613MetArg: 1.613 ± 0.039	1.272MetSer: 1.272 ± 0.033	1.634MetThr: 1.634 ± 0.038	1.778MetVal: 1.778 ± 0.043	0.184MetTrp: 0.184 ± 0.012	0.278MetTyr: 0.278 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.11AsnAla: 3.11 ± 0.062	0.23AsnCys: 0.23 ± 0.014	1.479AsnAsp: 1.479 ± 0.038	1.068AsnGlu: 1.068 ± 0.028	0.974AsnPhe: 0.974 ± 0.03	2.666AsnGly: 2.666 ± 0.063	0.585AsnHis: 0.585 ± 0.025	1.274AsnIle: 1.274 ± 0.03	0.639AsnLys: 0.639 ± 0.024	2.879AsnLeu: 2.879 ± 0.059	0.509AsnMet: 0.509 ± 0.019	0.878AsnAsn: 0.878 ± 0.034	1.861AsnPro: 1.861 ± 0.043	0.99AsnGln: 0.99 ± 0.031	1.786AsnArg: 1.786 ± 0.038	1.385AsnSer: 1.385 ± 0.052	1.496AsnThr: 1.496 ± 0.057	1.946AsnVal: 1.946 ± 0.044	0.415AsnTrp: 0.415 ± 0.018	0.868AsnTyr: 0.868 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	7.225ProAla: 7.225 ± 0.084	0.369ProCys: 0.369 ± 0.019	3.799ProAsp: 3.799 ± 0.061	2.949ProGlu: 2.949 ± 0.058	1.887ProPhe: 1.887 ± 0.041	5.309ProGly: 5.309 ± 0.076	1.118ProHis: 1.118 ± 0.027	2.01ProIle: 2.01 ± 0.044	1.523ProLys: 1.523 ± 0.042	5.401ProLeu: 5.401 ± 0.068	1.22ProMet: 1.22 ± 0.036	1.384ProAsn: 1.384 ± 0.037	3.156ProPro: 3.156 ± 0.062	1.877ProGln: 1.877 ± 0.039	3.021ProArg: 3.021 ± 0.054	2.823ProSer: 2.823 ± 0.052	2.906ProThr: 2.906 ± 0.047	4.388ProVal: 4.388 ± 0.067	0.798ProTrp: 0.798 ± 0.032	1.369ProTyr: 1.369 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	5.424GlnAla: 5.424 ± 0.097	0.223GlnCys: 0.223 ± 0.014	1.954GlnAsp: 1.954 ± 0.051	1.542GlnGlu: 1.542 ± 0.04	1.085GlnPhe: 1.085 ± 0.03	3.633GlnGly: 3.633 ± 0.095	0.968GlnHis: 0.968 ± 0.04	1.609GlnIle: 1.609 ± 0.039	0.94GlnLys: 0.94 ± 0.029	3.285GlnLeu: 3.285 ± 0.062	0.896GlnMet: 0.896 ± 0.031	0.968GlnAsn: 0.968 ± 0.033	2.272GlnPro: 2.272 ± 0.048	1.697GlnGln: 1.697 ± 0.061	3.043GlnArg: 3.043 ± 0.076	1.803GlnSer: 1.803 ± 0.039	2.015GlnThr: 2.015 ± 0.049	3.28GlnVal: 3.28 ± 0.069	0.43GlnTrp: 0.43 ± 0.019	0.809GlnTyr: 0.809 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	7.804ArgAla: 7.804 ± 0.1	0.548ArgCys: 0.548 ± 0.022	4.1ArgAsp: 4.1 ± 0.067	3.224ArgGlu: 3.224 ± 0.06	2.618ArgPhe: 2.618 ± 0.04	5.005ArgGly: 5.005 ± 0.075	2.258ArgHis: 2.258 ± 0.055	3.249ArgIle: 3.249 ± 0.056	1.829ArgLys: 1.829 ± 0.046	8.266ArgLeu: 8.266 ± 0.097	1.677ArgMet: 1.677 ± 0.04	1.719ArgAsn: 1.719 ± 0.037	4.031ArgPro: 4.031 ± 0.07	3.406ArgGln: 3.406 ± 0.065	6.366ArgArg: 6.366 ± 0.108	3.139ArgSer: 3.139 ± 0.067	3.595ArgThr: 3.595 ± 0.056	4.89ArgVal: 4.89 ± 0.078	1.131ArgTrp: 1.131 ± 0.031	2.017ArgTyr: 2.017 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	5.814SerAla: 5.814 ± 0.078	0.41SerCys: 0.41 ± 0.021	2.794SerAsp: 2.794 ± 0.055	1.929SerGlu: 1.929 ± 0.046	2.026SerPhe: 2.026 ± 0.054	5.103SerGly: 5.103 ± 0.106	1.063SerHis: 1.063 ± 0.031	2.246SerIle: 2.246 ± 0.054	1.208SerLys: 1.208 ± 0.033	5.43SerLeu: 5.43 ± 0.076	1.11SerMet: 1.11 ± 0.031	1.492SerAsn: 1.492 ± 0.045	2.946SerPro: 2.946 ± 0.055	1.756SerGln: 1.756 ± 0.039	3.269SerArg: 3.269 ± 0.057	2.954SerSer: 2.954 ± 0.078	2.948SerThr: 2.948 ± 0.07	3.869SerVal: 3.869 ± 0.066	0.807SerTrp: 0.807 ± 0.029	1.545SerTyr: 1.545 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	7.27ThrAla: 7.27 ± 0.104	0.401ThrCys: 0.401 ± 0.018	3.261ThrAsp: 3.261 ± 0.068	2.444ThrGlu: 2.444 ± 0.052	1.951ThrPhe: 1.951 ± 0.043	5.69ThrGly: 5.69 ± 0.124	1.105ThrHis: 1.105 ± 0.029	2.69ThrIle: 2.69 ± 0.07	1.36ThrLys: 1.36 ± 0.04	6.561ThrLeu: 6.561 ± 0.113	1.092ThrMet: 1.092 ± 0.033	1.576ThrAsn: 1.576 ± 0.046	3.722ThrPro: 3.722 ± 0.06	1.848ThrGln: 1.848 ± 0.044	3.332ThrArg: 3.332 ± 0.058	2.884ThrSer: 2.884 ± 0.065	3.404ThrThr: 3.404 ± 0.097	5.125ThrVal: 5.125 ± 0.081	0.777ThrTrp: 0.777 ± 0.031	1.53ThrTyr: 1.53 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	9.95ValAla: 9.95 ± 0.135	0.528ValCys: 0.528 ± 0.021	4.969ValAsp: 4.969 ± 0.079	4.11ValGlu: 4.11 ± 0.064	2.319ValPhe: 2.319 ± 0.045	6.702ValGly: 6.702 ± 0.116	1.586ValHis: 1.586 ± 0.042	3.172ValIle: 3.172 ± 0.058	2.135ValLys: 2.135 ± 0.049	7.204ValLeu: 7.204 ± 0.094	1.709ValMet: 1.709 ± 0.038	2.143ValAsn: 2.143 ± 0.054	4.181ValPro: 4.181 ± 0.06	2.768ValGln: 2.768 ± 0.067	5.158ValArg: 5.158 ± 0.069	4.069ValSer: 4.069 ± 0.072	5.102ValThr: 5.102 ± 0.087	6.337ValVal: 6.337 ± 0.088	0.909ValTrp: 0.909 ± 0.03	1.594ValTyr: 1.594 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.363TrpAla: 1.363 ± 0.035	0.126TrpCys: 0.126 ± 0.011	0.729TrpAsp: 0.729 ± 0.027	0.517TrpGlu: 0.517 ± 0.019	0.481TrpPhe: 0.481 ± 0.02	1.054TrpGly: 1.054 ± 0.029	0.374TrpHis: 0.374 ± 0.017	0.533TrpIle: 0.533 ± 0.02	0.398TrpLys: 0.398 ± 0.02	1.532TrpLeu: 1.532 ± 0.041	0.327TrpMet: 0.327 ± 0.016	0.471TrpAsn: 0.471 ± 0.02	0.735TrpPro: 0.735 ± 0.027	0.615TrpGln: 0.615 ± 0.023	1.272TrpArg: 1.272 ± 0.033	0.829TrpSer: 0.829 ± 0.028	0.908TrpThr: 0.908 ± 0.028	0.9TrpVal: 0.9 ± 0.032	0.216TrpTrp: 0.216 ± 0.014	0.352TrpTyr: 0.352 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.789TyrAla: 2.789 ± 0.052	0.228TyrCys: 0.228 ± 0.016	1.713TyrAsp: 1.713 ± 0.061	1.152TyrGlu: 1.152 ± 0.029	0.965TyrPhe: 0.965 ± 0.031	2.437TyrGly: 2.437 ± 0.045	0.544TyrHis: 0.544 ± 0.023	0.968TyrIle: 0.968 ± 0.027	0.705TyrLys: 0.705 ± 0.025	2.39TyrLeu: 2.39 ± 0.047	0.466TyrMet: 0.466 ± 0.018	0.785TyrAsn: 0.785 ± 0.03	1.188TyrPro: 1.188 ± 0.034	1.025TyrGln: 1.025 ± 0.033	1.966TyrArg: 1.966 ± 0.042	1.431TyrSer: 1.431 ± 0.042	1.5TyrThr: 1.5 ± 0.041	1.706TyrVal: 1.706 ± 0.043	0.394TyrTrp: 0.394 ± 0.021	0.781TyrTyr: 0.781 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3357 proteins (1202703 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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