Mycobacterium phage MosMoris
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A023ZWA3|A0A023ZWA3_9CAUD HTH cro/C1-type domain-containing protein OS=Mycobacterium phage MosMoris OX=1471542 GN=22 PE=4 SV=1
MM1 pKa = 7.78 PKK3 pKa = 10.35 YY4 pKa = 10.2 DD5 pKa = 3.94 VEE7 pKa = 4.04 WEE9 pKa = 3.78 ARR11 pKa = 11.84 GRR13 pKa = 11.84 LVVEE17 pKa = 4.3 ADD19 pKa = 3.68 DD20 pKa = 4.52 PEE22 pKa = 4.15 EE23 pKa = 5.11 AEE25 pKa = 4.39 QIVSEE30 pKa = 4.15 AVSDD34 pKa = 4.46 FDD36 pKa = 3.42 HH37 pKa = 6.52 TMAEE41 pKa = 4.38 AIDD44 pKa = 4.03 VEE46 pKa = 4.65 SVDD49 pKa = 4.6 IISTEE54 pKa = 3.96 ATSPDD59 pKa = 3.63 YY60 pKa = 11.55 GEE62 pKa = 4.38 EE63 pKa = 4.11 SS64 pKa = 3.25
Molecular weight: 7.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.224
IPC2_protein 3.656
IPC_protein 3.567
Toseland 3.389
ProMoST 3.732
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.478
Rodwell 3.414
Grimsley 3.312
Solomon 3.516
Lehninger 3.478
Nozaki 3.681
DTASelect 3.821
Thurlkill 3.452
EMBOSS 3.49
Sillero 3.694
Patrickios 3.223
IPC_peptide 3.516
IPC2_peptide 3.668
IPC2.peptide.svr19 3.687
Protein with the highest isoelectric point:
>tr|A0A023ZWR7|A0A023ZWR7_9CAUD Uncharacterized protein OS=Mycobacterium phage MosMoris OX=1471542 GN=18 PE=4 SV=1
MM1 pKa = 7.44 SNRR4 pKa = 11.84 RR5 pKa = 11.84 TVRR8 pKa = 11.84 IRR10 pKa = 11.84 IEE12 pKa = 4.03 SNKK15 pKa = 10.26 AKK17 pKa = 10.11 TSFWYY22 pKa = 9.43 TLVAGNGNTIMRR34 pKa = 11.84 STAKK38 pKa = 9.45 YY39 pKa = 4.77 TTEE42 pKa = 3.97 WNCRR46 pKa = 11.84 TAATRR51 pKa = 11.84 LCDD54 pKa = 3.71 ALRR57 pKa = 11.84 FSPLAVEE64 pKa = 4.4 VQDD67 pKa = 3.66 PKK69 pKa = 10.58 TRR71 pKa = 11.84 EE72 pKa = 3.43 ISTLEE77 pKa = 4.25 RR78 pKa = 11.84 YY79 pKa = 8.41 PSSPLKK85 pKa = 10.65 VFQKK89 pKa = 7.5 TTQTRR94 pKa = 11.84 SS95 pKa = 2.92
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.706
IPC_protein 10.452
Toseland 10.672
ProMoST 10.335
Dawson 10.774
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 11.023
Grimsley 10.818
Solomon 10.877
Lehninger 10.847
Nozaki 10.657
DTASelect 10.467
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.789
IPC_peptide 10.877
IPC2_peptide 9.516
IPC2.peptide.svr19 8.55
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
111
0
111
20368
40
1849
183.5
20.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.854 ± 0.348
1.223 ± 0.186
6.564 ± 0.222
6.721 ± 0.281
3.343 ± 0.15
8.214 ± 0.46
2.16 ± 0.147
4.537 ± 0.191
3.834 ± 0.247
8.322 ± 0.259
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.656 ± 0.117
3.515 ± 0.166
5.828 ± 0.248
3.618 ± 0.225
6.77 ± 0.376
5.44 ± 0.215
5.651 ± 0.282
6.746 ± 0.222
2.293 ± 0.147
2.71 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here