Amino acid dipepetide frequency for Mycobacterium phage MosMoris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.618AlaAla: 12.618 ± 1.272
0.933AlaCys: 0.933 ± 0.218
6.923AlaAsp: 6.923 ± 0.584
6.334AlaGlu: 6.334 ± 0.616
2.897AlaPhe: 2.897 ± 0.372
6.776AlaGly: 6.776 ± 0.586
2.209AlaHis: 2.209 ± 0.357
4.37AlaIle: 4.37 ± 0.506
4.468AlaLys: 4.468 ± 0.607
8.936AlaLeu: 8.936 ± 0.767
2.651AlaMet: 2.651 ± 0.276
3.486AlaAsn: 3.486 ± 0.539
4.91AlaPro: 4.91 ± 0.523
3.584AlaGln: 3.584 ± 0.518
6.088AlaArg: 6.088 ± 0.571
4.37AlaSer: 4.37 ± 0.596
5.892AlaThr: 5.892 ± 0.57
5.99AlaVal: 5.99 ± 0.636
2.16AlaTrp: 2.16 ± 0.364
2.946AlaTyr: 2.946 ± 0.391
0.0AlaXaa: 0.0 ± 0.0
Cys
0.835CysAla: 0.835 ± 0.211
0.245CysCys: 0.245 ± 0.14
1.227CysAsp: 1.227 ± 0.363
0.589CysGlu: 0.589 ± 0.165
0.245CysPhe: 0.245 ± 0.105
1.718CysGly: 1.718 ± 0.452
0.442CysHis: 0.442 ± 0.156
0.933CysIle: 0.933 ± 0.224
0.295CysLys: 0.295 ± 0.118
0.442CysLeu: 0.442 ± 0.153
0.147CysMet: 0.147 ± 0.082
0.638CysAsn: 0.638 ± 0.188
0.786CysPro: 0.786 ± 0.193
0.589CysGln: 0.589 ± 0.223
0.786CysArg: 0.786 ± 0.212
0.54CysSer: 0.54 ± 0.201
0.442CysThr: 0.442 ± 0.159
0.835CysVal: 0.835 ± 0.204
0.196CysTrp: 0.196 ± 0.101
0.295CysTyr: 0.295 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
6.186AspAla: 6.186 ± 0.519
0.736AspCys: 0.736 ± 0.263
5.352AspAsp: 5.352 ± 0.731
5.401AspGlu: 5.401 ± 0.648
2.16AspPhe: 2.16 ± 0.363
4.615AspGly: 4.615 ± 0.614
1.178AspHis: 1.178 ± 0.225
3.928AspIle: 3.928 ± 0.509
2.111AspLys: 2.111 ± 0.352
5.843AspLeu: 5.843 ± 0.506
2.013AspMet: 2.013 ± 0.325
1.62AspAsn: 1.62 ± 0.313
4.763AspPro: 4.763 ± 0.577
1.718AspGln: 1.718 ± 0.364
3.977AspArg: 3.977 ± 0.457
2.308AspSer: 2.308 ± 0.369
3.191AspThr: 3.191 ± 0.464
4.468AspVal: 4.468 ± 0.491
1.522AspTrp: 1.522 ± 0.283
2.553AspTyr: 2.553 ± 0.363
0.0AspXaa: 0.0 ± 0.0
Glu
7.119GluAla: 7.119 ± 0.737
0.736GluCys: 0.736 ± 0.312
3.879GluAsp: 3.879 ± 0.546
4.861GluGlu: 4.861 ± 0.537
3.339GluPhe: 3.339 ± 0.442
4.615GluGly: 4.615 ± 0.465
1.571GluHis: 1.571 ± 0.325
2.7GluIle: 2.7 ± 0.379
2.308GluLys: 2.308 ± 0.418
5.99GluLeu: 5.99 ± 0.61
2.602GluMet: 2.602 ± 0.392
2.013GluAsn: 2.013 ± 0.413
2.602GluPro: 2.602 ± 0.475
2.7GluGln: 2.7 ± 0.354
5.401GluArg: 5.401 ± 0.598
3.437GluSer: 3.437 ± 0.414
3.339GluThr: 3.339 ± 0.348
4.566GluVal: 4.566 ± 0.6
1.62GluTrp: 1.62 ± 0.286
1.817GluTyr: 1.817 ± 0.368
0.0GluXaa: 0.0 ± 0.0
Phe
2.357PheAla: 2.357 ± 0.3
0.54PheCys: 0.54 ± 0.176
2.602PheAsp: 2.602 ± 0.285
2.553PheGlu: 2.553 ± 0.346
0.982PhePhe: 0.982 ± 0.22
3.388PheGly: 3.388 ± 0.472
0.736PheHis: 0.736 ± 0.177
1.718PheIle: 1.718 ± 0.307
1.669PheLys: 1.669 ± 0.303
2.651PheLeu: 2.651 ± 0.42
0.835PheMet: 0.835 ± 0.199
1.375PheAsn: 1.375 ± 0.259
1.227PhePro: 1.227 ± 0.269
1.08PheGln: 1.08 ± 0.209
2.062PheArg: 2.062 ± 0.322
1.277PheSer: 1.277 ± 0.247
2.308PheThr: 2.308 ± 0.385
2.111PheVal: 2.111 ± 0.324
0.835PheTrp: 0.835 ± 0.238
1.129PheTyr: 1.129 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
6.874GlyAla: 6.874 ± 0.863
0.638GlyCys: 0.638 ± 0.195
5.45GlyAsp: 5.45 ± 0.622
4.124GlyGlu: 4.124 ± 0.482
3.437GlyPhe: 3.437 ± 0.466
8.396GlyGly: 8.396 ± 1.245
1.375GlyHis: 1.375 ± 0.261
3.633GlyIle: 3.633 ± 0.507
4.026GlyLys: 4.026 ± 0.555
6.481GlyLeu: 6.481 ± 0.619
1.915GlyMet: 1.915 ± 0.321
3.29GlyAsn: 3.29 ± 0.466
3.977GlyPro: 3.977 ± 0.611
3.191GlyGln: 3.191 ± 0.448
5.254GlyArg: 5.254 ± 0.677
5.155GlySer: 5.155 ± 0.623
4.566GlyThr: 4.566 ± 0.763
5.401GlyVal: 5.401 ± 0.625
2.16GlyTrp: 2.16 ± 0.372
2.799GlyTyr: 2.799 ± 0.371
0.0GlyXaa: 0.0 ± 0.0
His
1.866HisAla: 1.866 ± 0.353
0.147HisCys: 0.147 ± 0.084
1.571HisAsp: 1.571 ± 0.392
1.817HisGlu: 1.817 ± 0.302
0.344HisPhe: 0.344 ± 0.114
1.964HisGly: 1.964 ± 0.298
0.933HisHis: 0.933 ± 0.242
0.884HisIle: 0.884 ± 0.206
0.638HisLys: 0.638 ± 0.149
2.357HisLeu: 2.357 ± 0.302
0.344HisMet: 0.344 ± 0.108
0.884HisAsn: 0.884 ± 0.18
1.915HisPro: 1.915 ± 0.259
0.589HisGln: 0.589 ± 0.162
1.129HisArg: 1.129 ± 0.252
1.031HisSer: 1.031 ± 0.208
0.982HisThr: 0.982 ± 0.236
1.08HisVal: 1.08 ± 0.206
0.344HisTrp: 0.344 ± 0.119
0.786HisTyr: 0.786 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
5.057IleAla: 5.057 ± 0.495
0.786IleCys: 0.786 ± 0.215
3.633IleAsp: 3.633 ± 0.459
3.584IleGlu: 3.584 ± 0.461
1.227IlePhe: 1.227 ± 0.247
3.928IleGly: 3.928 ± 0.522
1.178IleHis: 1.178 ± 0.281
1.62IleIle: 1.62 ± 0.307
1.62IleLys: 1.62 ± 0.262
3.388IleLeu: 3.388 ± 0.443
0.835IleMet: 0.835 ± 0.197
1.62IleAsn: 1.62 ± 0.389
2.75IlePro: 2.75 ± 0.447
1.522IleGln: 1.522 ± 0.221
3.044IleArg: 3.044 ± 0.33
2.455IleSer: 2.455 ± 0.369
2.504IleThr: 2.504 ± 0.489
2.897IleVal: 2.897 ± 0.377
0.736IleTrp: 0.736 ± 0.203
0.982IleTyr: 0.982 ± 0.258
0.0IleXaa: 0.0 ± 0.0
Lys
4.517LysAla: 4.517 ± 0.623
0.638LysCys: 0.638 ± 0.168
1.277LysAsp: 1.277 ± 0.272
1.915LysGlu: 1.915 ± 0.43
1.424LysPhe: 1.424 ± 0.285
2.897LysGly: 2.897 ± 0.323
1.031LysHis: 1.031 ± 0.25
1.571LysIle: 1.571 ± 0.293
2.308LysLys: 2.308 ± 0.495
3.535LysLeu: 3.535 ± 0.477
1.129LysMet: 1.129 ± 0.195
1.031LysAsn: 1.031 ± 0.285
2.7LysPro: 2.7 ± 0.486
1.718LysGln: 1.718 ± 0.341
3.142LysArg: 3.142 ± 0.458
1.62LysSer: 1.62 ± 0.308
1.817LysThr: 1.817 ± 0.351
2.504LysVal: 2.504 ± 0.346
0.933LysTrp: 0.933 ± 0.197
0.638LysTyr: 0.638 ± 0.226
0.0LysXaa: 0.0 ± 0.0
Leu
7.905LeuAla: 7.905 ± 0.633
1.129LeuCys: 1.129 ± 0.317
6.579LeuAsp: 6.579 ± 0.702
5.695LeuGlu: 5.695 ± 0.556
2.553LeuPhe: 2.553 ± 0.512
6.285LeuGly: 6.285 ± 0.903
1.375LeuHis: 1.375 ± 0.254
3.682LeuIle: 3.682 ± 0.45
2.995LeuLys: 2.995 ± 0.476
6.825LeuLeu: 6.825 ± 0.623
1.964LeuMet: 1.964 ± 0.291
2.75LeuAsn: 2.75 ± 0.403
5.941LeuPro: 5.941 ± 0.712
2.7LeuGln: 2.7 ± 0.37
5.794LeuArg: 5.794 ± 0.545
4.763LeuSer: 4.763 ± 0.495
5.499LeuThr: 5.499 ± 0.513
5.303LeuVal: 5.303 ± 0.432
1.277LeuTrp: 1.277 ± 0.243
2.209LeuTyr: 2.209 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
3.142MetAla: 3.142 ± 0.43
0.442MetCys: 0.442 ± 0.152
0.982MetAsp: 0.982 ± 0.237
1.277MetGlu: 1.277 ± 0.263
1.277MetPhe: 1.277 ± 0.268
2.062MetGly: 2.062 ± 0.331
0.491MetHis: 0.491 ± 0.175
1.08MetIle: 1.08 ± 0.239
0.982MetLys: 0.982 ± 0.201
1.473MetLeu: 1.473 ± 0.246
0.589MetMet: 0.589 ± 0.153
1.129MetAsn: 1.129 ± 0.236
1.866MetPro: 1.866 ± 0.292
0.393MetGln: 0.393 ± 0.146
1.915MetArg: 1.915 ± 0.277
2.308MetSer: 2.308 ± 0.397
2.455MetThr: 2.455 ± 0.397
1.571MetVal: 1.571 ± 0.353
0.884MetTrp: 0.884 ± 0.171
0.245MetTyr: 0.245 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
3.83AsnAla: 3.83 ± 0.391
0.491AsnCys: 0.491 ± 0.189
1.964AsnAsp: 1.964 ± 0.271
2.111AsnGlu: 2.111 ± 0.338
1.227AsnPhe: 1.227 ± 0.267
3.584AsnGly: 3.584 ± 0.479
0.638AsnHis: 0.638 ± 0.18
1.62AsnIle: 1.62 ± 0.285
1.031AsnLys: 1.031 ± 0.262
2.651AsnLeu: 2.651 ± 0.445
0.491AsnMet: 0.491 ± 0.144
1.326AsnAsn: 1.326 ± 0.271
2.7AsnPro: 2.7 ± 0.459
1.62AsnGln: 1.62 ± 0.289
2.209AsnArg: 2.209 ± 0.352
1.817AsnSer: 1.817 ± 0.382
2.013AsnThr: 2.013 ± 0.356
2.111AsnVal: 2.111 ± 0.337
0.982AsnTrp: 0.982 ± 0.198
0.736AsnTyr: 0.736 ± 0.228
0.0AsnXaa: 0.0 ± 0.0
Pro
4.321ProAla: 4.321 ± 0.91
0.589ProCys: 0.589 ± 0.171
3.633ProAsp: 3.633 ± 0.45
4.91ProGlu: 4.91 ± 0.663
1.964ProPhe: 1.964 ± 0.293
5.548ProGly: 5.548 ± 0.855
1.522ProHis: 1.522 ± 0.3
2.062ProIle: 2.062 ± 0.378
1.915ProLys: 1.915 ± 0.371
4.075ProLeu: 4.075 ± 0.423
1.424ProMet: 1.424 ± 0.296
2.602ProAsn: 2.602 ± 0.359
3.732ProPro: 3.732 ± 0.457
2.308ProGln: 2.308 ± 0.344
3.191ProArg: 3.191 ± 0.448
4.517ProSer: 4.517 ± 0.588
3.486ProThr: 3.486 ± 0.558
3.633ProVal: 3.633 ± 0.477
1.129ProTrp: 1.129 ± 0.228
1.718ProTyr: 1.718 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
3.584GlnAla: 3.584 ± 0.486
0.491GlnCys: 0.491 ± 0.154
1.571GlnAsp: 1.571 ± 0.31
2.209GlnGlu: 2.209 ± 0.294
0.933GlnPhe: 0.933 ± 0.174
2.259GlnGly: 2.259 ± 0.417
0.638GlnHis: 0.638 ± 0.181
2.013GlnIle: 2.013 ± 0.368
1.768GlnLys: 1.768 ± 0.337
3.781GlnLeu: 3.781 ± 0.61
1.178GlnMet: 1.178 ± 0.224
0.687GlnAsn: 0.687 ± 0.156
1.718GlnPro: 1.718 ± 0.243
1.915GlnGln: 1.915 ± 0.388
3.191GlnArg: 3.191 ± 0.445
2.013GlnSer: 2.013 ± 0.376
1.817GlnThr: 1.817 ± 0.288
2.553GlnVal: 2.553 ± 0.349
1.08GlnTrp: 1.08 ± 0.275
0.786GlnTyr: 0.786 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
6.481ArgAla: 6.481 ± 0.59
1.08ArgCys: 1.08 ± 0.292
4.272ArgAsp: 4.272 ± 0.53
4.321ArgGlu: 4.321 ± 0.435
2.357ArgPhe: 2.357 ± 0.403
5.254ArgGly: 5.254 ± 0.567
1.326ArgHis: 1.326 ± 0.302
3.339ArgIle: 3.339 ± 0.341
3.191ArgLys: 3.191 ± 0.497
6.137ArgLeu: 6.137 ± 0.604
1.522ArgMet: 1.522 ± 0.266
2.16ArgAsn: 2.16 ± 0.324
3.29ArgPro: 3.29 ± 0.343
2.504ArgGln: 2.504 ± 0.402
6.186ArgArg: 6.186 ± 0.735
2.995ArgSer: 2.995 ± 0.37
2.75ArgThr: 2.75 ± 0.384
4.321ArgVal: 4.321 ± 0.558
2.259ArgTrp: 2.259 ± 0.35
1.964ArgTyr: 1.964 ± 0.382
0.0ArgXaa: 0.0 ± 0.0
Ser
5.401SerAla: 5.401 ± 0.637
0.344SerCys: 0.344 ± 0.156
3.928SerAsp: 3.928 ± 0.52
3.29SerGlu: 3.29 ± 0.386
1.669SerPhe: 1.669 ± 0.372
4.763SerGly: 4.763 ± 0.676
1.178SerHis: 1.178 ± 0.275
2.553SerIle: 2.553 ± 0.361
1.424SerLys: 1.424 ± 0.264
4.468SerLeu: 4.468 ± 0.528
1.964SerMet: 1.964 ± 0.344
1.669SerAsn: 1.669 ± 0.376
2.995SerPro: 2.995 ± 0.386
1.964SerGln: 1.964 ± 0.288
3.879SerArg: 3.879 ± 0.608
3.682SerSer: 3.682 ± 0.547
3.486SerThr: 3.486 ± 0.506
3.584SerVal: 3.584 ± 0.432
1.178SerTrp: 1.178 ± 0.215
0.982SerTyr: 0.982 ± 0.21
0.0SerXaa: 0.0 ± 0.0
Thr
5.45ThrAla: 5.45 ± 0.603
0.442ThrCys: 0.442 ± 0.171
3.682ThrAsp: 3.682 ± 0.471
2.848ThrGlu: 2.848 ± 0.382
1.817ThrPhe: 1.817 ± 0.292
5.106ThrGly: 5.106 ± 0.617
1.178ThrHis: 1.178 ± 0.265
2.504ThrIle: 2.504 ± 0.326
1.866ThrLys: 1.866 ± 0.265
5.057ThrLeu: 5.057 ± 0.615
1.571ThrMet: 1.571 ± 0.288
2.357ThrAsn: 2.357 ± 0.388
3.781ThrPro: 3.781 ± 0.569
2.111ThrGln: 2.111 ± 0.3
3.535ThrArg: 3.535 ± 0.446
2.7ThrSer: 2.7 ± 0.498
3.339ThrThr: 3.339 ± 0.508
4.664ThrVal: 4.664 ± 0.62
1.227ThrTrp: 1.227 ± 0.287
1.277ThrTyr: 1.277 ± 0.241
0.0ThrXaa: 0.0 ± 0.0
Val
7.021ValAla: 7.021 ± 0.7
0.933ValCys: 0.933 ± 0.242
3.633ValAsp: 3.633 ± 0.421
5.597ValGlu: 5.597 ± 0.506
2.062ValPhe: 2.062 ± 0.299
4.861ValGly: 4.861 ± 0.572
1.08ValHis: 1.08 ± 0.218
3.142ValIle: 3.142 ± 0.505
2.308ValLys: 2.308 ± 0.333
5.155ValLeu: 5.155 ± 0.457
1.915ValMet: 1.915 ± 0.388
2.75ValAsn: 2.75 ± 0.389
4.419ValPro: 4.419 ± 0.493
2.16ValGln: 2.16 ± 0.37
3.977ValArg: 3.977 ± 0.438
4.517ValSer: 4.517 ± 0.377
3.732ValThr: 3.732 ± 0.448
5.99ValVal: 5.99 ± 0.725
0.638ValTrp: 0.638 ± 0.171
1.522ValTyr: 1.522 ± 0.273
0.0ValXaa: 0.0 ± 0.0
Trp
1.522TrpAla: 1.522 ± 0.305
0.442TrpCys: 0.442 ± 0.143
1.768TrpAsp: 1.768 ± 0.329
1.669TrpGlu: 1.669 ± 0.308
0.638TrpPhe: 0.638 ± 0.168
1.718TrpGly: 1.718 ± 0.25
0.933TrpHis: 0.933 ± 0.23
0.638TrpIle: 0.638 ± 0.16
0.835TrpLys: 0.835 ± 0.203
1.866TrpLeu: 1.866 ± 0.293
0.687TrpMet: 0.687 ± 0.181
0.736TrpAsn: 0.736 ± 0.22
0.835TrpPro: 0.835 ± 0.182
0.884TrpGln: 0.884 ± 0.176
1.522TrpArg: 1.522 ± 0.257
1.375TrpSer: 1.375 ± 0.272
1.375TrpThr: 1.375 ± 0.284
2.013TrpVal: 2.013 ± 0.359
0.736TrpTrp: 0.736 ± 0.185
0.736TrpTyr: 0.736 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.455TyrAla: 2.455 ± 0.397
0.442TyrCys: 0.442 ± 0.172
1.718TyrAsp: 1.718 ± 0.295
2.111TyrGlu: 2.111 ± 0.429
0.884TyrPhe: 0.884 ± 0.223
2.406TyrGly: 2.406 ± 0.329
0.589TyrHis: 0.589 ± 0.213
1.375TyrIle: 1.375 ± 0.299
0.589TyrLys: 0.589 ± 0.193
2.111TyrLeu: 2.111 ± 0.374
0.736TyrMet: 0.736 ± 0.168
1.031TyrAsn: 1.031 ± 0.186
1.375TyrPro: 1.375 ± 0.273
0.933TyrGln: 0.933 ± 0.185
1.424TyrArg: 1.424 ± 0.259
1.522TyrSer: 1.522 ± 0.326
1.522TyrThr: 1.522 ± 0.304
1.866TyrVal: 1.866 ± 0.311
1.031TyrTrp: 1.031 ± 0.252
0.982TyrTyr: 0.982 ± 0.244
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 111 proteins (20368 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski