Jembrana disease virus (JDV)
Average proteome isoelectric point is 7.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q82854-2|TAT-2_JEMBR Isoform of Q82854 Isoform Short of Protein Tat OS=Jembrana disease virus OX=36370 GN=tat PE=1 SV=1
MM1 pKa = 7.62 SNLGAQTRR9 pKa = 11.84 YY10 pKa = 10.02 QLLNAQEE17 pKa = 4.39 DD18 pKa = 4.18 TDD20 pKa = 4.18 PAGDD24 pKa = 3.77 GDD26 pKa = 3.87 QPDD29 pKa = 3.78 DD30 pKa = 4.17 HH31 pKa = 7.54 RR32 pKa = 11.84 SGDD35 pKa = 3.81 TPRR38 pKa = 11.84 SGVPSGGWSQKK49 pKa = 10.24 LSEE52 pKa = 4.29 GKK54 pKa = 10.58 KK55 pKa = 9.57 IGCLILRR62 pKa = 11.84 TEE64 pKa = 4.0 WQNWRR69 pKa = 11.84 NDD71 pKa = 3.0 LRR73 pKa = 11.84 TLRR76 pKa = 11.84 WLTLGGKK83 pKa = 8.99 ILQLPLSLLVLLVRR97 pKa = 11.84 ILLHH101 pKa = 6.13 ILSPTFQNQRR111 pKa = 11.84 GWTVGRR117 pKa = 11.84 KK118 pKa = 7.86 GTGGDD123 pKa = 3.65 DD124 pKa = 3.56 RR125 pKa = 11.84 EE126 pKa = 4.33 LSPEE130 pKa = 4.19 LEE132 pKa = 4.26 YY133 pKa = 11.2 LSWTGSSQEE142 pKa = 4.01 MVEE145 pKa = 3.98 MRR147 pKa = 11.84 DD148 pKa = 3.73 LKK150 pKa = 11.23 EE151 pKa = 3.55 EE152 pKa = 5.15 DD153 pKa = 3.38 IPEE156 pKa = 4.04 EE157 pKa = 4.43 GIRR160 pKa = 11.84 PVEE163 pKa = 3.96 MM164 pKa = 4.93
Molecular weight: 18.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.035
IPC2_protein 5.003
IPC_protein 4.889
Toseland 4.762
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.711
Rodwell 4.749
Grimsley 4.673
Solomon 4.838
Lehninger 4.8
Nozaki 4.952
DTASelect 5.105
Thurlkill 4.762
EMBOSS 4.736
Sillero 5.016
Patrickios 4.482
IPC_peptide 4.851
IPC2_peptide 5.016
IPC2.peptide.svr19 5.005
Protein with the highest isoelectric point:
>sp|Q82855-2|REV-2_JEMBR Isoform of Q82855 Isoform Short of Protein Rev OS=Jembrana disease virus OX=36370 GN=rev PE=4 SV=1
MM1 pKa = 7.63 PGPWATTLTFPGHH14 pKa = 6.14 NGGFGGGPKK23 pKa = 9.97 CWLFWNTCAGPRR35 pKa = 11.84 RR36 pKa = 11.84 VCPKK40 pKa = 9.6 CSCPICVWHH49 pKa = 6.79 CQLCFLQKK57 pKa = 10.9 GLGIRR62 pKa = 11.84 HH63 pKa = 6.72 DD64 pKa = 3.68 GRR66 pKa = 11.84 RR67 pKa = 11.84 KK68 pKa = 9.85 KK69 pKa = 10.56 RR70 pKa = 11.84 GTRR73 pKa = 11.84 GKK75 pKa = 10.3 GRR77 pKa = 11.84 KK78 pKa = 7.47 IHH80 pKa = 5.47 YY81 pKa = 8.97 ARR83 pKa = 11.84 SITEE87 pKa = 3.89 SGGQRR92 pKa = 11.84 APNCAA97 pKa = 3.33
Molecular weight: 10.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.385
IPC_protein 9.531
Toseland 10.526
ProMoST 10.058
Dawson 10.599
Bjellqvist 10.277
Wikipedia 10.716
Rodwell 11.008
Grimsley 10.613
Solomon 10.672
Lehninger 10.657
Nozaki 10.613
DTASelect 10.218
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.555
Patrickios 10.789
IPC_peptide 10.687
IPC2_peptide 9.794
IPC2.peptide.svr19 8.298
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
2
9
3635
97
1432
403.9
45.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.025 ± 0.678
2.476 ± 0.626
4.539 ± 0.353
7.455 ± 0.749
2.503 ± 0.307
8.171 ± 1.144
2.669 ± 0.25
5.475 ± 0.805
6.162 ± 0.915
9.106 ± 0.748
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.871 ± 0.178
3.659 ± 0.36
6.052 ± 0.499
4.677 ± 0.412
7.345 ± 1.004
5.117 ± 0.754
5.695 ± 0.442
5.695 ± 0.863
2.806 ± 0.345
2.503 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here