Chitinophaga cymbidii
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5222 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A512RN88|A0A512RN88_9BACT DSBA oxidoreductase OS=Chitinophaga cymbidii OX=1096750 GN=CCY01nite_34270 PE=4 SV=1
MM1 pKa = 7.58 EE2 pKa = 4.95 EE3 pKa = 4.45 LYY5 pKa = 10.82 AGKK8 pKa = 7.96 WTGQYY13 pKa = 10.38 TYY15 pKa = 10.53 GDD17 pKa = 3.84 EE18 pKa = 4.31 YY19 pKa = 11.6 AEE21 pKa = 4.19 EE22 pKa = 4.19 LRR24 pKa = 11.84 GLSVPFIIDD33 pKa = 3.19 IQVDD37 pKa = 3.63 DD38 pKa = 4.89 DD39 pKa = 4.93 GEE41 pKa = 4.32 LEE43 pKa = 4.29 GMCMDD48 pKa = 3.83 MDD50 pKa = 4.33 EE51 pKa = 4.65 EE52 pKa = 4.0 QLEE55 pKa = 4.44 AVVKK59 pKa = 10.35 GYY61 pKa = 10.27 IEE63 pKa = 4.77 DD64 pKa = 4.5 GYY66 pKa = 10.82 ISFIKK71 pKa = 10.12 KK72 pKa = 10.31 YY73 pKa = 10.02 MEE75 pKa = 4.33 TDD77 pKa = 3.09 PGGEE81 pKa = 3.9 PVFMDD86 pKa = 2.39 IHH88 pKa = 7.38 YY89 pKa = 9.93 YY90 pKa = 10.51 GEE92 pKa = 4.09 YY93 pKa = 9.83 RR94 pKa = 11.84 DD95 pKa = 3.75 GAFAGEE101 pKa = 4.62 WEE103 pKa = 4.63 IEE105 pKa = 3.7 WSVINEE111 pKa = 3.74 AGSRR115 pKa = 11.84 VEE117 pKa = 3.95 YY118 pKa = 10.39 LSTGIWEE125 pKa = 4.12 MRR127 pKa = 11.84 RR128 pKa = 11.84 LL129 pKa = 3.55
Molecular weight: 14.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.935
IPC_protein 3.884
Toseland 3.706
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.719
Rodwell 3.706
Grimsley 3.617
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.088
Thurlkill 3.732
EMBOSS 3.732
Sillero 3.986
Patrickios 0.744
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A512RTA2|A0A512RTA2_9BACT Uncharacterized protein OS=Chitinophaga cymbidii OX=1096750 GN=CCY01nite_51880 PE=4 SV=1
MM1 pKa = 7.5 AKK3 pKa = 8.19 ITRR6 pKa = 11.84 TIRR9 pKa = 11.84 VRR11 pKa = 11.84 GSKK14 pKa = 9.44 TMTGFVFFLTAVFFFVIGWMKK35 pKa = 9.58 EE36 pKa = 3.66 AKK38 pKa = 10.24 ALMWVAVAICTLLTLLSLVRR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 TVVTLDD66 pKa = 3.75 DD67 pKa = 3.49 KK68 pKa = 11.37 GIRR71 pKa = 11.84 YY72 pKa = 8.79 KK73 pKa = 11.07 SPFWNLHH80 pKa = 4.58 FLWRR84 pKa = 11.84 DD85 pKa = 3.16 VRR87 pKa = 11.84 ACGVYY92 pKa = 8.48 YY93 pKa = 10.37 VRR95 pKa = 11.84 NRR97 pKa = 11.84 EE98 pKa = 4.25 VYY100 pKa = 9.36 PHH102 pKa = 6.29 QPGQGDD108 pKa = 3.92 LALHH112 pKa = 6.55 DD113 pKa = 4.54 KK114 pKa = 10.2 WPLTIFVSTRR124 pKa = 11.84 MHH126 pKa = 6.67 YY127 pKa = 10.3 FPQRR131 pKa = 11.84 QNRR134 pKa = 11.84 FINRR138 pKa = 11.84 NCIHH142 pKa = 5.92 FRR144 pKa = 11.84 WNRR147 pKa = 11.84 EE148 pKa = 3.36 AWSVIARR155 pKa = 11.84 NVNGEE160 pKa = 4.33 CKK162 pKa = 10.61 ACITPP167 pKa = 4.03
Molecular weight: 19.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.78
IPC_protein 10.687
Toseland 10.76
ProMoST 10.526
Dawson 10.862
Bjellqvist 10.613
Wikipedia 11.096
Rodwell 11.023
Grimsley 10.906
Solomon 10.994
Lehninger 10.965
Nozaki 10.76
DTASelect 10.599
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.804
Patrickios 10.745
IPC_peptide 10.994
IPC2_peptide 9.853
IPC2.peptide.svr19 8.444
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5222
0
5222
1995864
41
3863
382.2
42.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.372 ± 0.034
0.774 ± 0.012
5.329 ± 0.021
5.682 ± 0.028
4.609 ± 0.019
7.307 ± 0.037
2.007 ± 0.02
6.242 ± 0.029
5.776 ± 0.028
9.342 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.455 ± 0.014
4.954 ± 0.03
4.345 ± 0.018
3.956 ± 0.02
5.056 ± 0.022
5.955 ± 0.025
5.807 ± 0.033
6.511 ± 0.021
1.376 ± 0.013
4.145 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here