Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei;

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47088 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R4IS80|A0A0R4IS80_DANRE Bromodomain and PHD finger-containing 3a OS=Danio rerio OX=7955 GN=brpf3a PE=4 SV=1
VV1 pKa = 6.0YY2 pKa = 9.97AQPRR6 pKa = 11.84KK7 pKa = 8.64RR8 pKa = 11.84TQRR11 pKa = 11.84IQNTTEE17 pKa = 3.89DD18 pKa = 3.81EE19 pKa = 4.71DD20 pKa = 4.88YY21 pKa = 11.17INPDD25 pKa = 3.46DD26 pKa = 5.26DD27 pKa = 5.05GNPIMDD33 pKa = 3.75HH34 pKa = 6.78TDD36 pKa = 3.28TEE38 pKa = 4.79SYY40 pKa = 10.94EE41 pKa = 4.03IMSGSDD47 pKa = 3.37CIYY50 pKa = 10.49NNSGFTAVHH59 pKa = 6.59DD60 pKa = 4.65LEE62 pKa = 7.26DD63 pKa = 4.51GDD65 pKa = 4.37SYY67 pKa = 11.94EE68 pKa = 4.93VMDD71 pKa = 4.73EE72 pKa = 4.22VPII75 pKa = 4.54

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R4IQ63|A0A0R4IQ63_DANRE Cytochrome P450 family 2 subfamily K polypeptide16 OS=Danio rerio OX=7955 GN=cyp2k16 PE=1 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25706

21382

47088

24679081

7

29152

524.1

58.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.191 ± 0.01

2.336 ± 0.01

5.245 ± 0.008

6.955 ± 0.015

3.724 ± 0.009

5.981 ± 0.014

2.678 ± 0.006

4.7 ± 0.008

5.93 ± 0.013

9.487 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.005

4.039 ± 0.007

5.413 ± 0.016

4.777 ± 0.012

5.484 ± 0.009

8.848 ± 0.016

5.681 ± 0.012

6.245 ± 0.011

1.116 ± 0.004

2.764 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski