Amino acid dipepetide frequency for Danio rerio (Zebrafish) (Brachydanio rerio)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.381AlaAla: 5.381 ± 0.024	1.236AlaCys: 1.236 ± 0.009	3.091AlaAsp: 3.091 ± 0.012	4.538AlaGlu: 4.538 ± 0.022	2.376AlaPhe: 2.376 ± 0.012	3.968AlaGly: 3.968 ± 0.018	1.494AlaHis: 1.494 ± 0.009	2.652AlaIle: 2.652 ± 0.012	3.204AlaLys: 3.204 ± 0.017	6.299AlaLeu: 6.299 ± 0.022	1.459AlaMet: 1.459 ± 0.008	2.162AlaAsn: 2.162 ± 0.011	3.126AlaPro: 3.126 ± 0.02	2.882AlaGln: 2.882 ± 0.013	2.901AlaArg: 2.901 ± 0.012	5.148AlaSer: 5.148 ± 0.017	3.135AlaThr: 3.135 ± 0.013	4.812AlaVal: 4.812 ± 0.017	0.599AlaTrp: 0.599 ± 0.006	1.439AlaTyr: 1.439 ± 0.009	0.005AlaXaa: 0.005 ± 0.0
Cys	1.223CysAla: 1.223 ± 0.009	0.671CysCys: 0.671 ± 0.008	1.123CysAsp: 1.123 ± 0.011	1.353CysGlu: 1.353 ± 0.012	0.933CysPhe: 0.933 ± 0.007	1.797CysGly: 1.797 ± 0.017	0.652CysHis: 0.652 ± 0.006	1.012CysIle: 1.012 ± 0.008	1.275CysLys: 1.275 ± 0.01	2.138CysLeu: 2.138 ± 0.014	0.498CysMet: 0.498 ± 0.005	0.89CysAsn: 0.89 ± 0.007	1.242CysPro: 1.242 ± 0.013	1.046CysGln: 1.046 ± 0.009	1.312CysArg: 1.312 ± 0.01	2.165CysSer: 2.165 ± 0.016	1.357CysThr: 1.357 ± 0.012	1.718CysVal: 1.718 ± 0.014	0.325CysTrp: 0.325 ± 0.004	0.624CysTyr: 0.624 ± 0.005	0.004CysXaa: 0.004 ± 0.0
Asp	2.945AspAla: 2.945 ± 0.013	1.178AspCys: 1.178 ± 0.011	3.14AspAsp: 3.14 ± 0.019	3.907AspGlu: 3.907 ± 0.015	2.101AspPhe: 2.101 ± 0.011	3.641AspGly: 3.641 ± 0.022	1.256AspHis: 1.256 ± 0.008	2.775AspIle: 2.775 ± 0.014	2.782AspLys: 2.782 ± 0.013	5.233AspLeu: 5.233 ± 0.018	1.243AspMet: 1.243 ± 0.007	1.913AspAsn: 1.913 ± 0.012	2.804AspPro: 2.804 ± 0.014	2.018AspGln: 2.018 ± 0.009	2.598AspArg: 2.598 ± 0.015	4.568AspSer: 4.568 ± 0.018	2.671AspThr: 2.671 ± 0.012	3.433AspVal: 3.433 ± 0.016	0.702AspTrp: 0.702 ± 0.006	1.541AspTyr: 1.541 ± 0.008	0.006AspXaa: 0.006 ± 0.001
Glu	4.321GluAla: 4.321 ± 0.02	1.333GluCys: 1.333 ± 0.012	4.537GluAsp: 4.537 ± 0.018	7.604GluGlu: 7.604 ± 0.037	2.053GluPhe: 2.053 ± 0.012	3.806GluGly: 3.806 ± 0.018	1.548GluHis: 1.548 ± 0.009	3.213GluIle: 3.213 ± 0.015	5.228GluLys: 5.228 ± 0.026	6.13GluLeu: 6.13 ± 0.023	1.823GluMet: 1.823 ± 0.011	3.056GluAsn: 3.056 ± 0.014	2.76GluPro: 2.76 ± 0.017	3.165GluGln: 3.165 ± 0.016	4.247GluArg: 4.247 ± 0.023	4.82GluSer: 4.82 ± 0.02	3.563GluThr: 3.563 ± 0.018	4.073GluVal: 4.073 ± 0.018	0.658GluTrp: 0.658 ± 0.006	1.608GluTyr: 1.608 ± 0.012	0.008GluXaa: 0.008 ± 0.001
Phe	1.899PheAla: 1.899 ± 0.011	0.961PheCys: 0.961 ± 0.008	1.776PheAsp: 1.776 ± 0.011	1.965PheGlu: 1.965 ± 0.012	1.6PhePhe: 1.6 ± 0.011	2.208PheGly: 2.208 ± 0.011	0.992PheHis: 0.992 ± 0.007	2.148PheIle: 2.148 ± 0.012	1.931PheLys: 1.931 ± 0.01	3.866PheLeu: 3.866 ± 0.019	0.833PheMet: 0.833 ± 0.007	1.611PheAsn: 1.611 ± 0.01	1.788PhePro: 1.788 ± 0.011	1.665PheGln: 1.665 ± 0.009	1.969PheArg: 1.969 ± 0.012	3.544PheSer: 3.544 ± 0.017	2.499PheThr: 2.499 ± 0.015	2.267PheVal: 2.267 ± 0.012	0.463PheTrp: 0.463 ± 0.005	1.244PheTyr: 1.244 ± 0.009	0.005PheXaa: 0.005 ± 0.0
Gly	3.551GlyAla: 3.551 ± 0.018	1.218GlyCys: 1.218 ± 0.01	3.132GlyAsp: 3.132 ± 0.015	3.998GlyGlu: 3.998 ± 0.022	2.464GlyPhe: 2.464 ± 0.014	4.416GlyGly: 4.416 ± 0.028	1.604GlyHis: 1.604 ± 0.009	2.68GlyIle: 2.68 ± 0.013	3.774GlyLys: 3.774 ± 0.018	5.272GlyLeu: 5.272 ± 0.02	1.411GlyMet: 1.411 ± 0.01	2.477GlyAsn: 2.477 ± 0.014	2.98GlyPro: 2.98 ± 0.033	2.603GlyGln: 2.603 ± 0.015	3.322GlyArg: 3.322 ± 0.017	5.393GlySer: 5.393 ± 0.023	3.182GlyThr: 3.182 ± 0.016	3.857GlyVal: 3.857 ± 0.017	0.709GlyTrp: 0.709 ± 0.007	1.764GlyTyr: 1.764 ± 0.013	0.003GlyXaa: 0.003 ± 0.0
His	1.309HisAla: 1.309 ± 0.009	0.81HisCys: 0.81 ± 0.007	0.982HisAsp: 0.982 ± 0.007	1.309HisGlu: 1.309 ± 0.007	1.071HisPhe: 1.071 ± 0.008	1.467HisGly: 1.467 ± 0.01	0.99HisHis: 0.99 ± 0.011	1.399HisIle: 1.399 ± 0.008	1.352HisLys: 1.352 ± 0.009	2.757HisLeu: 2.757 ± 0.014	0.886HisMet: 0.886 ± 0.011	1.063HisAsn: 1.063 ± 0.006	1.521HisPro: 1.521 ± 0.011	1.296HisGln: 1.296 ± 0.009	1.558HisArg: 1.558 ± 0.008	2.415HisSer: 2.415 ± 0.013	2.007HisThr: 2.007 ± 0.019	1.426HisVal: 1.426 ± 0.007	0.33HisTrp: 0.33 ± 0.004	0.83HisTyr: 0.83 ± 0.006	0.005HisXaa: 0.005 ± 0.0
Ile	2.594IleAla: 2.594 ± 0.012	1.158IleCys: 1.158 ± 0.009	2.208IleAsp: 2.208 ± 0.012	2.651IleGlu: 2.651 ± 0.017	1.943IlePhe: 1.943 ± 0.012	2.36IleGly: 2.36 ± 0.011	1.583IleHis: 1.583 ± 0.012	2.654IleIle: 2.654 ± 0.014	2.87IleLys: 2.87 ± 0.016	4.403IleLeu: 4.403 ± 0.018	1.15IleMet: 1.15 ± 0.008	2.152IleAsn: 2.152 ± 0.011	2.543IlePro: 2.543 ± 0.011	2.347IleGln: 2.347 ± 0.011	2.547IleArg: 2.547 ± 0.012	4.188IleSer: 4.188 ± 0.016	3.006IleThr: 3.006 ± 0.017	2.604IleVal: 2.604 ± 0.014	0.514IleTrp: 0.514 ± 0.005	1.522IleTyr: 1.522 ± 0.012	0.006IleXaa: 0.006 ± 0.0
Lys	3.688LysAla: 3.688 ± 0.016	1.136LysCys: 1.136 ± 0.011	3.371LysAsp: 3.371 ± 0.018	4.86LysGlu: 4.86 ± 0.025	1.665LysPhe: 1.665 ± 0.009	3.24LysGly: 3.24 ± 0.021	1.592LysHis: 1.592 ± 0.009	2.785LysIle: 2.785 ± 0.013	4.638LysLys: 4.638 ± 0.025	5.275LysLeu: 5.275 ± 0.022	1.476LysMet: 1.476 ± 0.009	2.45LysAsn: 2.45 ± 0.012	3.226LysPro: 3.226 ± 0.019	2.753LysGln: 2.753 ± 0.014	3.549LysArg: 3.549 ± 0.015	4.483LysSer: 4.483 ± 0.017	3.488LysThr: 3.488 ± 0.016	3.454LysVal: 3.454 ± 0.016	0.582LysTrp: 0.582 ± 0.007	1.581LysTyr: 1.581 ± 0.01	0.008LysXaa: 0.008 ± 0.001
Leu	5.572LeuAla: 5.572 ± 0.019	2.204LeuCys: 2.204 ± 0.014	4.994LeuAsp: 4.994 ± 0.018	6.47LeuGlu: 6.47 ± 0.025	3.456LeuPhe: 3.456 ± 0.017	4.725LeuGly: 4.725 ± 0.018	2.685LeuHis: 2.685 ± 0.014	4.172LeuIle: 4.172 ± 0.017	6.075LeuLys: 6.075 ± 0.022	9.817LeuLeu: 9.817 ± 0.038	2.175LeuMet: 2.175 ± 0.012	3.974LeuAsn: 3.974 ± 0.015	4.945LeuPro: 4.945 ± 0.02	5.678LeuGln: 5.678 ± 0.023	5.45LeuArg: 5.45 ± 0.02	8.498LeuSer: 8.498 ± 0.03	5.271LeuThr: 5.271 ± 0.016	5.06LeuVal: 5.06 ± 0.018	0.991LeuTrp: 0.991 ± 0.007	2.641LeuTyr: 2.641 ± 0.014	0.012LeuXaa: 0.012 ± 0.001
Met	1.817MetAla: 1.817 ± 0.011	0.543MetCys: 0.543 ± 0.006	1.399MetAsp: 1.399 ± 0.007	1.969MetGlu: 1.969 ± 0.011	0.885MetPhe: 0.885 ± 0.007	1.353MetGly: 1.353 ± 0.011	0.506MetHis: 0.506 ± 0.005	0.997MetIle: 0.997 ± 0.007	1.6MetLys: 1.6 ± 0.008	2.014MetLeu: 2.014 ± 0.01	0.752MetMet: 0.752 ± 0.006	0.997MetAsn: 0.997 ± 0.007	1.096MetPro: 1.096 ± 0.011	1.038MetGln: 1.038 ± 0.007	1.364MetArg: 1.364 ± 0.01	1.94MetSer: 1.94 ± 0.01	1.25MetThr: 1.25 ± 0.008	1.452MetVal: 1.452 ± 0.008	0.264MetTrp: 0.264 ± 0.003	0.659MetTyr: 0.659 ± 0.006	0.002MetXaa: 0.002 ± 0.0
Asn	2.23AsnAla: 2.23 ± 0.01	0.959AsnCys: 0.959 ± 0.01	1.845AsnAsp: 1.845 ± 0.011	2.332AsnGlu: 2.332 ± 0.013	1.486AsnPhe: 1.486 ± 0.011	2.854AsnGly: 2.854 ± 0.018	1.054AsnHis: 1.054 ± 0.008	2.316AsnIle: 2.316 ± 0.011	2.37AsnLys: 2.37 ± 0.014	3.864AsnLeu: 3.864 ± 0.015	1.071AsnMet: 1.071 ± 0.007	1.927AsnAsn: 1.927 ± 0.012	2.279AsnPro: 2.279 ± 0.011	1.867AsnGln: 1.867 ± 0.01	2.076AsnArg: 2.076 ± 0.01	3.375AsnSer: 3.375 ± 0.018	2.5AsnThr: 2.5 ± 0.013	2.38AsnVal: 2.38 ± 0.014	0.449AsnTrp: 0.449 ± 0.005	1.154AsnTyr: 1.154 ± 0.007	0.005AsnXaa: 0.005 ± 0.001
Pro	3.86ProAla: 3.86 ± 0.02	1.015ProCys: 1.015 ± 0.009	2.755ProAsp: 2.755 ± 0.014	3.716ProGlu: 3.716 ± 0.018	1.911ProPhe: 1.911 ± 0.011	3.654ProGly: 3.654 ± 0.041	1.396ProHis: 1.396 ± 0.009	1.938ProIle: 1.938 ± 0.011	2.615ProLys: 2.615 ± 0.019	4.597ProLeu: 4.597 ± 0.018	1.019ProMet: 1.019 ± 0.008	1.991ProAsn: 1.991 ± 0.011	4.825ProPro: 4.825 ± 0.039	2.567ProGln: 2.567 ± 0.017	2.456ProArg: 2.456 ± 0.013	5.263ProSer: 5.263 ± 0.028	2.868ProThr: 2.868 ± 0.018	3.764ProVal: 3.764 ± 0.02	0.493ProTrp: 0.493 ± 0.005	1.42ProTyr: 1.42 ± 0.01	0.005ProXaa: 0.005 ± 0.0
Gln	2.965GlnAla: 2.965 ± 0.014	1.288GlnCys: 1.288 ± 0.012	2.456GlnAsp: 2.456 ± 0.011	3.444GlnGlu: 3.444 ± 0.015	1.397GlnPhe: 1.397 ± 0.009	2.462GlnGly: 2.462 ± 0.015	1.456GlnHis: 1.456 ± 0.01	2.17GlnIle: 2.17 ± 0.01	2.871GlnLys: 2.871 ± 0.015	4.331GlnLeu: 4.331 ± 0.018	1.224GlnMet: 1.224 ± 0.008	1.988GlnAsn: 1.988 ± 0.01	2.412GlnPro: 2.412 ± 0.016	3.238GlnGln: 3.238 ± 0.033	2.951GlnArg: 2.951 ± 0.013	3.819GlnSer: 3.819 ± 0.017	2.826GlnThr: 2.826 ± 0.012	2.617GlnVal: 2.617 ± 0.013	0.553GlnTrp: 0.553 ± 0.005	1.29GlnTyr: 1.29 ± 0.008	0.006GlnXaa: 0.006 ± 0.0
Arg	3.223ArgAla: 3.223 ± 0.012	1.223ArgCys: 1.223 ± 0.01	2.898ArgAsp: 2.898 ± 0.016	3.868ArgGlu: 3.868 ± 0.02	2.005ArgPhe: 2.005 ± 0.01	3.132ArgGly: 3.132 ± 0.018	1.496ArgHis: 1.496 ± 0.01	2.638ArgIle: 2.638 ± 0.013	3.653ArgLys: 3.653 ± 0.016	5.141ArgLeu: 5.141 ± 0.02	1.294ArgMet: 1.294 ± 0.008	2.166ArgAsn: 2.166 ± 0.01	2.739ArgPro: 2.739 ± 0.013	2.571ArgGln: 2.571 ± 0.012	4.153ArgArg: 4.153 ± 0.019	4.533ArgSer: 4.533 ± 0.022	2.754ArgThr: 2.754 ± 0.012	3.209ArgVal: 3.209 ± 0.016	0.631ArgTrp: 0.631 ± 0.006	1.507ArgTyr: 1.507 ± 0.009	0.008ArgXaa: 0.008 ± 0.001
Ser	5.744SerAla: 5.744 ± 0.019	2.004SerCys: 2.004 ± 0.013	4.544SerAsp: 4.544 ± 0.017	5.286SerGlu: 5.286 ± 0.018	3.382SerPhe: 3.382 ± 0.018	5.572SerGly: 5.572 ± 0.021	2.208SerHis: 2.208 ± 0.012	3.654SerIle: 3.654 ± 0.018	4.254SerLys: 4.254 ± 0.017	8.278SerLeu: 8.278 ± 0.027	1.856SerMet: 1.856 ± 0.01	3.257SerAsn: 3.257 ± 0.018	5.509SerPro: 5.509 ± 0.031	3.849SerGln: 3.849 ± 0.015	4.537SerArg: 4.537 ± 0.022	10.635SerSer: 10.635 ± 0.047	4.954SerThr: 4.954 ± 0.029	5.797SerVal: 5.797 ± 0.021	0.98SerTrp: 0.98 ± 0.008	2.166SerTyr: 2.166 ± 0.012	0.012SerXaa: 0.012 ± 0.001
Thr	3.873ThrAla: 3.873 ± 0.023	1.541ThrCys: 1.541 ± 0.016	3.076ThrAsp: 3.076 ± 0.012	3.969ThrGlu: 3.969 ± 0.018	2.127ThrPhe: 2.127 ± 0.011	3.804ThrGly: 3.804 ± 0.017	1.653ThrHis: 1.653 ± 0.015	2.483ThrIle: 2.483 ± 0.012	2.635ThrLys: 2.635 ± 0.016	5.52ThrLeu: 5.52 ± 0.02	1.159ThrMet: 1.159 ± 0.009	2.014ThrAsn: 2.014 ± 0.013	3.539ThrPro: 3.539 ± 0.024	2.64ThrGln: 2.64 ± 0.012	2.414ThrArg: 2.414 ± 0.01	4.803ThrSer: 4.803 ± 0.023	3.275ThrThr: 3.275 ± 0.038	4.224ThrVal: 4.224 ± 0.018	0.619ThrTrp: 0.619 ± 0.009	1.431ThrTyr: 1.431 ± 0.009	0.007ThrXaa: 0.007 ± 0.001
Val	3.696ValAla: 3.696 ± 0.014	1.853ValCys: 1.853 ± 0.017	3.137ValAsp: 3.137 ± 0.013	3.96ValGlu: 3.96 ± 0.018	2.697ValPhe: 2.697 ± 0.013	3.128ValGly: 3.128 ± 0.013	1.593ValHis: 1.593 ± 0.008	3.072ValIle: 3.072 ± 0.014	3.908ValLys: 3.908 ± 0.019	6.289ValLeu: 6.289 ± 0.02	1.539ValMet: 1.539 ± 0.008	2.551ValAsn: 2.551 ± 0.015	3.126ValPro: 3.126 ± 0.016	2.861ValGln: 2.861 ± 0.012	3.094ValArg: 3.094 ± 0.014	5.449ValSer: 5.449 ± 0.021	3.777ValThr: 3.777 ± 0.027	4.081ValVal: 4.081 ± 0.019	0.741ValTrp: 0.741 ± 0.006	1.886ValTyr: 1.886 ± 0.012	0.008ValXaa: 0.008 ± 0.001
Trp	0.631TrpAla: 0.631 ± 0.005	0.248TrpCys: 0.248 ± 0.003	0.611TrpAsp: 0.611 ± 0.006	0.696TrpGlu: 0.696 ± 0.006	0.47TrpPhe: 0.47 ± 0.005	0.566TrpGly: 0.566 ± 0.006	0.271TrpHis: 0.271 ± 0.003	0.65TrpIle: 0.65 ± 0.006	0.709TrpLys: 0.709 ± 0.005	1.067TrpLeu: 1.067 ± 0.008	0.335TrpMet: 0.335 ± 0.004	0.487TrpAsn: 0.487 ± 0.005	0.408TrpPro: 0.408 ± 0.005	0.469TrpGln: 0.469 ± 0.004	0.703TrpArg: 0.703 ± 0.006	0.989TrpSer: 0.989 ± 0.007	0.704TrpThr: 0.704 ± 0.007	0.634TrpVal: 0.634 ± 0.006	0.169TrpTrp: 0.169 ± 0.003	0.34TrpTyr: 0.34 ± 0.004	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.377TyrAla: 1.377 ± 0.008	0.775TyrCys: 0.775 ± 0.007	1.374TyrAsp: 1.374 ± 0.008	1.652TyrGlu: 1.652 ± 0.009	1.205TyrPhe: 1.205 ± 0.008	1.648TyrGly: 1.648 ± 0.01	0.748TyrHis: 0.748 ± 0.006	1.589TyrIle: 1.589 ± 0.011	1.55TyrLys: 1.55 ± 0.011	2.565TyrLeu: 2.565 ± 0.014	0.691TyrMet: 0.691 ± 0.006	1.262TyrAsn: 1.262 ± 0.01	1.254TyrPro: 1.254 ± 0.009	1.208TyrGln: 1.208 ± 0.007	1.636TyrArg: 1.636 ± 0.01	2.438TyrSer: 2.438 ± 0.011	1.712TyrThr: 1.712 ± 0.01	1.576TyrVal: 1.576 ± 0.011	0.386TyrTrp: 0.386 ± 0.005	0.993TyrTyr: 0.993 ± 0.007	0.004TyrXaa: 0.004 ± 0.0
Xaa	0.007XaaAla: 0.007 ± 0.0	0.003XaaCys: 0.003 ± 0.0	0.006XaaAsp: 0.006 ± 0.0	0.007XaaGlu: 0.007 ± 0.001	0.005XaaPhe: 0.005 ± 0.0	0.008XaaGly: 0.008 ± 0.001	0.003XaaHis: 0.003 ± 0.0	0.005XaaIle: 0.005 ± 0.0	0.007XaaLys: 0.007 ± 0.001	0.011XaaLeu: 0.011 ± 0.001	0.004XaaMet: 0.004 ± 0.0	0.006XaaAsn: 0.006 ± 0.0	0.006XaaPro: 0.006 ± 0.0	0.005XaaGln: 0.005 ± 0.0	0.006XaaArg: 0.006 ± 0.001	0.012XaaSer: 0.012 ± 0.001	0.008XaaThr: 0.008 ± 0.001	0.008XaaVal: 0.008 ± 0.001	0.002XaaTrp: 0.002 ± 0.0	0.002XaaTyr: 0.002 ± 0.0	0.044XaaXaa: 0.044 ± 0.008

Statistics based on 47088 proteins (24679081 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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