Toxoplasma gondii

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 466 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1JSW7|Q1JSW7_TOXGO Uncharacterized protein OS=Toxoplasma gondii OX=5811 GN=TgIb.0340 PE=4 SV=1
MM1 pKa = 7.51LCPVGFYY8 pKa = 10.53CLEE11 pKa = 4.38GAQTPSEE18 pKa = 4.44CPAGRR23 pKa = 11.84QTAAAGAMRR32 pKa = 11.84EE33 pKa = 4.3EE34 pKa = 4.47EE35 pKa = 4.67CVPCEE40 pKa = 3.75PGFYY44 pKa = 10.15CPEE47 pKa = 4.74PGVSLPCDD55 pKa = 3.14AGYY58 pKa = 11.13VCLEE62 pKa = 4.3GATTATPADD71 pKa = 3.88TTTGKK76 pKa = 9.98QCDD79 pKa = 3.32AGFYY83 pKa = 10.11CPQGSFRR90 pKa = 11.84MLVSQQSGQMMM101 pKa = 4.5

Molecular weight: 10.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1JTG0|Q1JTG0_TOXGO Importin beta-1 subunit putative OS=Toxoplasma gondii OX=5811 GN=TgIa.0580 PE=4 SV=1
MM1 pKa = 6.93SQVSKK6 pKa = 11.04SLASHH11 pKa = 6.76ISASINRR18 pKa = 11.84TGKK21 pKa = 8.94MDD23 pKa = 3.93PVTRR27 pKa = 11.84RR28 pKa = 11.84FNPCLAFCVVNILLLCIGNLTYY50 pKa = 10.73DD51 pKa = 3.65PQSYY55 pKa = 10.31IILRR59 pKa = 11.84EE60 pKa = 3.89AFFQGQEE67 pKa = 4.0HH68 pKa = 6.58RR69 pKa = 11.84RR70 pKa = 11.84HH71 pKa = 6.0CALNLLSISNAYY83 pKa = 9.04AARR86 pKa = 11.84VPPNSGDD93 pKa = 3.37TARR96 pKa = 11.84PLTSSNAGRR105 pKa = 11.84RR106 pKa = 11.84SSSPTPPASTASNRR120 pKa = 11.84HH121 pKa = 5.85PPLHH125 pKa = 5.94QPSTEE130 pKa = 3.44RR131 pKa = 11.84RR132 pKa = 11.84LCHH135 pKa = 7.34DD136 pKa = 4.28GFQEE140 pKa = 4.07GRR142 pKa = 11.84NRR144 pKa = 11.84QHH146 pKa = 6.73RR147 pKa = 11.84RR148 pKa = 11.84AEE150 pKa = 4.23APASLPVFQDD160 pKa = 3.03VTIPTGVRR168 pKa = 11.84PPRR171 pKa = 11.84FHH173 pKa = 7.1CLWASRR179 pKa = 11.84KK180 pKa = 8.25QAALLVQRR188 pKa = 11.84VRR190 pKa = 11.84QGRR193 pKa = 11.84RR194 pKa = 11.84LLYY197 pKa = 9.68TSAIDD202 pKa = 3.49RR203 pKa = 11.84QGYY206 pKa = 9.06KK207 pKa = 9.75LARR210 pKa = 11.84MKK212 pKa = 10.48VPCARR217 pKa = 11.84KK218 pKa = 9.23FLDD221 pKa = 3.51DD222 pKa = 4.19CRR224 pKa = 11.84NTGEE228 pKa = 3.84GCYY231 pKa = 10.83NKK233 pKa = 9.33FHH235 pKa = 7.51CINIYY240 pKa = 10.5YY241 pKa = 7.74NTTVTHH247 pKa = 6.61TILRR251 pKa = 11.84TLLL254 pKa = 3.74

Molecular weight: 28.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

466

0

466

372232

68

12269

798.8

86.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.089 ± 0.103

2.059 ± 0.066

4.835 ± 0.068

7.995 ± 0.139

3.455 ± 0.055

7.599 ± 0.124

2.119 ± 0.035

2.383 ± 0.085

4.166 ± 0.11

8.947 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.508 ± 0.055

2.375 ± 0.051

6.214 ± 0.121

4.01 ± 0.066

8.121 ± 0.153

10.506 ± 0.229

5.107 ± 0.065

5.992 ± 0.112

1.007 ± 0.025

1.512 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski