Amino acid dipepetide frequency for Toxoplasma gondii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.58AlaAla: 13.58 ± 0.315
2.19AlaCys: 2.19 ± 0.112
4.18AlaAsp: 4.18 ± 0.113
7.399AlaGlu: 7.399 ± 0.185
3.707AlaPhe: 3.707 ± 0.114
7.922AlaGly: 7.922 ± 0.218
1.913AlaHis: 1.913 ± 0.074
2.015AlaIle: 2.015 ± 0.111
3.484AlaLys: 3.484 ± 0.115
8.758AlaLeu: 8.758 ± 0.227
1.351AlaMet: 1.351 ± 0.073
2.16AlaAsn: 2.16 ± 0.083
5.91AlaPro: 5.91 ± 0.228
3.353AlaGln: 3.353 ± 0.107
6.542AlaArg: 6.542 ± 0.193
12.447AlaSer: 12.447 ± 0.409
5.303AlaThr: 5.303 ± 0.139
6.397AlaVal: 6.397 ± 0.17
0.844AlaTrp: 0.844 ± 0.066
1.432AlaTyr: 1.432 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
1.735CysAla: 1.735 ± 0.08
0.586CysCys: 0.586 ± 0.044
0.819CysAsp: 0.819 ± 0.067
1.104CysGlu: 1.104 ± 0.077
0.903CysPhe: 0.903 ± 0.066
1.698CysGly: 1.698 ± 0.088
0.387CysHis: 0.387 ± 0.033
0.604CysIle: 0.604 ± 0.045
0.637CysLys: 0.637 ± 0.056
2.13CysLeu: 2.13 ± 0.104
0.357CysMet: 0.357 ± 0.035
0.443CysAsn: 0.443 ± 0.047
1.534CysPro: 1.534 ± 0.231
0.645CysGln: 0.645 ± 0.059
1.623CysArg: 1.623 ± 0.072
2.281CysSer: 2.281 ± 0.15
1.067CysThr: 1.067 ± 0.064
1.437CysVal: 1.437 ± 0.068
0.261CysTrp: 0.261 ± 0.028
0.344CysTyr: 0.344 ± 0.041
0.0CysXaa: 0.0 ± 0.0
Asp
5.652AspAla: 5.652 ± 0.139
0.935AspCys: 0.935 ± 0.064
2.915AspAsp: 2.915 ± 0.139
4.556AspGlu: 4.556 ± 0.159
1.851AspPhe: 1.851 ± 0.072
3.909AspGly: 3.909 ± 0.13
0.693AspHis: 0.693 ± 0.05
1.389AspIle: 1.389 ± 0.071
1.883AspLys: 1.883 ± 0.092
3.93AspLeu: 3.93 ± 0.109
0.83AspMet: 0.83 ± 0.06
1.048AspAsn: 1.048 ± 0.056
2.418AspPro: 2.418 ± 0.101
1.322AspGln: 1.322 ± 0.078
3.589AspArg: 3.589 ± 0.173
4.731AspSer: 4.731 ± 0.137
2.284AspThr: 2.284 ± 0.09
3.178AspVal: 3.178 ± 0.132
0.578AspTrp: 0.578 ± 0.036
0.664AspTyr: 0.664 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
8.557GluAla: 8.557 ± 0.223
1.042GluCys: 1.042 ± 0.067
4.844GluAsp: 4.844 ± 0.168
10.716GluGlu: 10.716 ± 0.43
1.636GluPhe: 1.636 ± 0.072
6.673GluGly: 6.673 ± 0.228
1.233GluHis: 1.233 ± 0.065
1.897GluIle: 1.897 ± 0.084
5.545GluLys: 5.545 ± 0.254
4.865GluLeu: 4.865 ± 0.132
1.292GluMet: 1.292 ± 0.079
2.289GluAsn: 2.289 ± 0.1
2.657GluPro: 2.657 ± 0.08
3.084GluGln: 3.084 ± 0.102
7.299GluArg: 7.299 ± 0.346
5.215GluSer: 5.215 ± 0.164
5.752GluThr: 5.752 ± 0.188
3.826GluVal: 3.826 ± 0.112
0.631GluTrp: 0.631 ± 0.042
0.9GluTyr: 0.9 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
3.087PheAla: 3.087 ± 0.108
0.956PheCys: 0.956 ± 0.073
1.76PheAsp: 1.76 ± 0.081
1.859PheGlu: 1.859 ± 0.098
1.899PhePhe: 1.899 ± 0.081
2.058PheGly: 2.058 ± 0.087
0.795PheHis: 0.795 ± 0.051
0.868PheIle: 0.868 ± 0.063
0.849PheLys: 0.849 ± 0.051
4.075PheLeu: 4.075 ± 0.128
0.478PheMet: 0.478 ± 0.053
0.698PheAsn: 0.698 ± 0.05
2.378PhePro: 2.378 ± 0.104
1.217PheGln: 1.217 ± 0.054
2.19PheArg: 2.19 ± 0.082
4.352PheSer: 4.352 ± 0.197
1.37PheThr: 1.37 ± 0.076
2.369PheVal: 2.369 ± 0.086
0.473PheTrp: 0.473 ± 0.039
0.814PheTyr: 0.814 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
7.399GlyAla: 7.399 ± 0.268
1.418GlyCys: 1.418 ± 0.074
4.978GlyAsp: 4.978 ± 0.151
7.358GlyGlu: 7.358 ± 0.275
2.308GlyPhe: 2.308 ± 0.107
7.541GlyGly: 7.541 ± 0.288
1.324GlyHis: 1.324 ± 0.078
1.864GlyIle: 1.864 ± 0.092
3.543GlyLys: 3.543 ± 0.134
5.263GlyLeu: 5.263 ± 0.17
1.153GlyMet: 1.153 ± 0.077
2.042GlyAsn: 2.042 ± 0.079
3.734GlyPro: 3.734 ± 0.196
2.474GlyGln: 2.474 ± 0.081
6.3GlyArg: 6.3 ± 0.257
6.877GlySer: 6.877 ± 0.217
3.863GlyThr: 3.863 ± 0.128
4.884GlyVal: 4.884 ± 0.136
0.666GlyTrp: 0.666 ± 0.04
0.999GlyTyr: 0.999 ± 0.071
0.0GlyXaa: 0.0 ± 0.0
His
2.563HisAla: 2.563 ± 0.085
0.398HisCys: 0.398 ± 0.035
0.723HisAsp: 0.723 ± 0.05
1.161HisGlu: 1.161 ± 0.055
0.862HisPhe: 0.862 ± 0.062
1.504HisGly: 1.504 ± 0.083
0.591HisHis: 0.591 ± 0.042
0.664HisIle: 0.664 ± 0.054
0.645HisLys: 0.645 ± 0.041
2.179HisLeu: 2.179 ± 0.085
0.322HisMet: 0.322 ± 0.042
0.451HisAsn: 0.451 ± 0.031
1.59HisPro: 1.59 ± 0.061
0.865HisGln: 0.865 ± 0.049
1.703HisArg: 1.703 ± 0.072
1.934HisSer: 1.934 ± 0.083
1.155HisThr: 1.155 ± 0.062
1.257HisVal: 1.257 ± 0.079
0.258HisTrp: 0.258 ± 0.026
0.368HisTyr: 0.368 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
2.028IleAla: 2.028 ± 0.101
0.682IleCys: 0.682 ± 0.058
1.359IleAsp: 1.359 ± 0.091
1.679IleGlu: 1.679 ± 0.095
1.075IlePhe: 1.075 ± 0.069
1.416IleGly: 1.416 ± 0.09
0.647IleHis: 0.647 ± 0.051
0.666IleIle: 0.666 ± 0.075
0.854IleLys: 0.854 ± 0.057
2.361IleLeu: 2.361 ± 0.098
0.414IleMet: 0.414 ± 0.042
0.607IleAsn: 0.607 ± 0.048
1.531IlePro: 1.531 ± 0.086
1.161IleGln: 1.161 ± 0.068
1.773IleArg: 1.773 ± 0.089
2.149IleSer: 2.149 ± 0.091
0.927IleThr: 0.927 ± 0.081
1.636IleVal: 1.636 ± 0.09
0.349IleTrp: 0.349 ± 0.035
0.516IleTyr: 0.516 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
3.804LysAla: 3.804 ± 0.124
0.58LysCys: 0.58 ± 0.052
2.106LysAsp: 2.106 ± 0.118
4.108LysGlu: 4.108 ± 0.195
0.924LysPhe: 0.924 ± 0.057
2.909LysGly: 2.909 ± 0.117
0.911LysHis: 0.911 ± 0.065
1.158LysIle: 1.158 ± 0.068
3.253LysLys: 3.253 ± 0.141
3.014LysLeu: 3.014 ± 0.124
0.811LysMet: 0.811 ± 0.054
1.491LysAsn: 1.491 ± 0.079
2.104LysPro: 2.104 ± 0.172
2.063LysGln: 2.063 ± 0.095
4.097LysArg: 4.097 ± 0.153
2.681LysSer: 2.681 ± 0.086
2.461LysThr: 2.461 ± 0.089
2.2LysVal: 2.2 ± 0.124
0.411LysTrp: 0.411 ± 0.032
0.578LysTyr: 0.578 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
7.984LeuAla: 7.984 ± 0.194
2.052LeuCys: 2.052 ± 0.086
3.745LeuAsp: 3.745 ± 0.113
5.601LeuGlu: 5.601 ± 0.147
3.565LeuPhe: 3.565 ± 0.138
5.378LeuGly: 5.378 ± 0.171
2.622LeuHis: 2.622 ± 0.094
1.915LeuIle: 1.915 ± 0.109
3.417LeuLys: 3.417 ± 0.155
10.604LeuLeu: 10.604 ± 0.327
1.22LeuMet: 1.22 ± 0.075
1.886LeuAsn: 1.886 ± 0.086
6.689LeuPro: 6.689 ± 0.213
4.623LeuGln: 4.623 ± 0.131
7.211LeuArg: 7.211 ± 0.187
9.54LeuSer: 9.54 ± 0.29
3.877LeuThr: 3.877 ± 0.132
4.897LeuVal: 4.897 ± 0.152
1.005LeuTrp: 1.005 ± 0.062
1.633LeuTyr: 1.633 ± 0.076
0.0LeuXaa: 0.0 ± 0.0
Met
1.429MetAla: 1.429 ± 0.071
0.253MetCys: 0.253 ± 0.032
0.725MetAsp: 0.725 ± 0.069
1.147MetGlu: 1.147 ± 0.094
0.47MetPhe: 0.47 ± 0.048
0.948MetGly: 0.948 ± 0.076
0.449MetHis: 0.449 ± 0.037
0.379MetIle: 0.379 ± 0.04
0.852MetLys: 0.852 ± 0.059
1.359MetLeu: 1.359 ± 0.083
0.32MetMet: 0.32 ± 0.039
0.414MetAsn: 0.414 ± 0.033
0.868MetPro: 0.868 ± 0.051
0.94MetGln: 0.94 ± 0.055
1.29MetArg: 1.29 ± 0.058
1.069MetSer: 1.069 ± 0.056
0.809MetThr: 0.809 ± 0.05
0.841MetVal: 0.841 ± 0.069
0.164MetTrp: 0.164 ± 0.024
0.352MetTyr: 0.352 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
2.767AsnAla: 2.767 ± 0.103
0.502AsnCys: 0.502 ± 0.042
1.042AsnAsp: 1.042 ± 0.062
1.872AsnGlu: 1.872 ± 0.083
0.895AsnPhe: 0.895 ± 0.053
2.02AsnGly: 2.02 ± 0.09
0.454AsnHis: 0.454 ± 0.033
0.736AsnIle: 0.736 ± 0.063
0.967AsnLys: 0.967 ± 0.068
2.13AsnLeu: 2.13 ± 0.096
0.373AsnMet: 0.373 ± 0.034
0.578AsnAsn: 0.578 ± 0.043
1.338AsnPro: 1.338 ± 0.067
0.768AsnGln: 0.768 ± 0.049
1.717AsnArg: 1.717 ± 0.085
2.122AsnSer: 2.122 ± 0.09
1.187AsnThr: 1.187 ± 0.073
1.574AsnVal: 1.574 ± 0.079
0.304AsnTrp: 0.304 ± 0.032
0.406AsnTyr: 0.406 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
6.563ProAla: 6.563 ± 0.188
1.265ProCys: 1.265 ± 0.101
2.547ProAsp: 2.547 ± 0.094
3.89ProGlu: 3.89 ± 0.124
2.321ProPhe: 2.321 ± 0.104
4.701ProGly: 4.701 ± 0.175
1.513ProHis: 1.513 ± 0.075
1.085ProIle: 1.085 ± 0.068
1.738ProLys: 1.738 ± 0.106
5.738ProLeu: 5.738 ± 0.195
0.596ProMet: 0.596 ± 0.048
1.236ProAsn: 1.236 ± 0.053
5.58ProPro: 5.58 ± 0.233
2.415ProGln: 2.415 ± 0.094
4.632ProArg: 4.632 ± 0.161
8.076ProSer: 8.076 ± 0.3
2.969ProThr: 2.969 ± 0.115
3.976ProVal: 3.976 ± 0.162
0.508ProTrp: 0.508 ± 0.044
0.793ProTyr: 0.793 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
3.705GlnAla: 3.705 ± 0.102
0.631GlnCys: 0.631 ± 0.058
1.615GlnAsp: 1.615 ± 0.077
3.449GlnGlu: 3.449 ± 0.118
1.101GlnPhe: 1.101 ± 0.055
2.754GlnGly: 2.754 ± 0.091
0.752GlnHis: 0.752 ± 0.052
1.171GlnIle: 1.171 ± 0.063
2.133GlnLys: 2.133 ± 0.115
3.882GlnLeu: 3.882 ± 0.129
0.844GlnMet: 0.844 ± 0.048
1.096GlnAsn: 1.096 ± 0.059
1.926GlnPro: 1.926 ± 0.075
2.66GlnGln: 2.66 ± 0.169
3.869GlnArg: 3.869 ± 0.123
2.909GlnSer: 2.909 ± 0.091
2.396GlnThr: 2.396 ± 0.083
2.286GlnVal: 2.286 ± 0.091
0.395GlnTrp: 0.395 ± 0.044
0.521GlnTyr: 0.521 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
6.415ArgAla: 6.415 ± 0.17
1.453ArgCys: 1.453 ± 0.062
4.242ArgAsp: 4.242 ± 0.182
8.143ArgGlu: 8.143 ± 0.367
2.251ArgPhe: 2.251 ± 0.089
6.926ArgGly: 6.926 ± 0.251
2.071ArgHis: 2.071 ± 0.098
1.856ArgIle: 1.856 ± 0.087
3.638ArgLys: 3.638 ± 0.142
7.06ArgLeu: 7.06 ± 0.196
1.362ArgMet: 1.362 ± 0.065
2.028ArgAsn: 2.028 ± 0.078
3.938ArgPro: 3.938 ± 0.129
3.796ArgGln: 3.796 ± 0.12
9.545ArgArg: 9.545 ± 0.401
6.359ArgSer: 6.359 ± 0.215
3.887ArgThr: 3.887 ± 0.127
4.392ArgVal: 4.392 ± 0.102
0.768ArgTrp: 0.768 ± 0.045
1.075ArgTyr: 1.075 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
10.171SerAla: 10.171 ± 0.258
2.243SerCys: 2.243 ± 0.143
3.944SerAsp: 3.944 ± 0.132
6.069SerGlu: 6.069 ± 0.203
3.898SerPhe: 3.898 ± 0.159
7.673SerGly: 7.673 ± 0.241
1.932SerHis: 1.932 ± 0.085
2.09SerIle: 2.09 ± 0.106
2.668SerLys: 2.668 ± 0.091
9.967SerLeu: 9.967 ± 0.336
1.169SerMet: 1.169 ± 0.072
1.964SerAsn: 1.964 ± 0.089
9.083SerPro: 9.083 ± 0.394
3.331SerGln: 3.331 ± 0.119
7.939SerArg: 7.939 ± 0.296
18.241SerSer: 18.241 ± 1.046
4.61SerThr: 4.61 ± 0.129
5.921SerVal: 5.921 ± 0.163
0.975SerTrp: 0.975 ± 0.051
1.177SerTyr: 1.177 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
5.247ThrAla: 5.247 ± 0.131
1.096ThrCys: 1.096 ± 0.086
2.361ThrAsp: 2.361 ± 0.095
3.877ThrGlu: 3.877 ± 0.165
1.553ThrPhe: 1.553 ± 0.073
4.261ThrGly: 4.261 ± 0.152
1.067ThrHis: 1.067 ± 0.056
1.147ThrIle: 1.147 ± 0.085
1.969ThrLys: 1.969 ± 0.106
4.317ThrLeu: 4.317 ± 0.125
0.736ThrMet: 0.736 ± 0.056
1.166ThrAsn: 1.166 ± 0.066
3.775ThrPro: 3.775 ± 0.101
2.16ThrGln: 2.16 ± 0.089
3.823ThrArg: 3.823 ± 0.12
5.026ThrSer: 5.026 ± 0.14
2.708ThrThr: 2.708 ± 0.147
3.471ThrVal: 3.471 ± 0.129
0.492ThrTrp: 0.492 ± 0.037
0.817ThrTyr: 0.817 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
6.125ValAla: 6.125 ± 0.154
1.598ValCys: 1.598 ± 0.07
3.339ValAsp: 3.339 ± 0.115
4.398ValGlu: 4.398 ± 0.13
2.337ValPhe: 2.337 ± 0.095
3.987ValGly: 3.987 ± 0.133
1.284ValHis: 1.284 ± 0.06
1.478ValIle: 1.478 ± 0.088
2.461ValLys: 2.461 ± 0.153
5.416ValLeu: 5.416 ± 0.191
0.83ValMet: 0.83 ± 0.076
1.429ValAsn: 1.429 ± 0.083
3.742ValPro: 3.742 ± 0.134
2.171ValGln: 2.171 ± 0.08
3.815ValArg: 3.815 ± 0.107
6.856ValSer: 6.856 ± 0.18
2.99ValThr: 2.99 ± 0.135
3.775ValVal: 3.775 ± 0.175
0.688ValTrp: 0.688 ± 0.043
1.198ValTyr: 1.198 ± 0.084
0.0ValXaa: 0.0 ± 0.0
Trp
0.763TrpAla: 0.763 ± 0.051
0.22TrpCys: 0.22 ± 0.031
0.43TrpAsp: 0.43 ± 0.038
0.615TrpGlu: 0.615 ± 0.037
0.328TrpPhe: 0.328 ± 0.032
0.626TrpGly: 0.626 ± 0.051
0.201TrpHis: 0.201 ± 0.021
0.341TrpIle: 0.341 ± 0.033
0.586TrpLys: 0.586 ± 0.045
0.919TrpLeu: 0.919 ± 0.052
0.258TrpMet: 0.258 ± 0.022
0.355TrpAsn: 0.355 ± 0.035
0.459TrpPro: 0.459 ± 0.041
0.521TrpGln: 0.521 ± 0.039
1.104TrpArg: 1.104 ± 0.068
0.86TrpSer: 0.86 ± 0.052
0.65TrpThr: 0.65 ± 0.044
0.532TrpVal: 0.532 ± 0.044
0.148TrpTrp: 0.148 ± 0.021
0.153TrpTyr: 0.153 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.311TyrAla: 1.311 ± 0.066
0.492TyrCys: 0.492 ± 0.09
0.68TyrAsp: 0.68 ± 0.047
0.951TyrGlu: 0.951 ± 0.07
0.661TyrPhe: 0.661 ± 0.044
1.085TyrGly: 1.085 ± 0.062
0.355TyrHis: 0.355 ± 0.03
0.51TyrIle: 0.51 ± 0.059
0.543TyrLys: 0.543 ± 0.044
1.496TyrLeu: 1.496 ± 0.068
0.357TyrMet: 0.357 ± 0.037
0.373TyrAsn: 0.373 ± 0.04
0.887TyrPro: 0.887 ± 0.062
0.527TyrGln: 0.527 ± 0.038
1.15TyrArg: 1.15 ± 0.063
1.338TyrSer: 1.338 ± 0.077
0.803TyrThr: 0.803 ± 0.05
1.067TyrVal: 1.067 ± 0.07
0.153TyrTrp: 0.153 ± 0.024
0.387TyrTyr: 0.387 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 466 proteins (372232 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski