Amino acid dipepetide frequency for Pseudomonas fluorescens (strain F113)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.162AlaAla: 12.162 ± 0.125	1.172AlaCys: 1.172 ± 0.024	5.774AlaAsp: 5.774 ± 0.063	6.385AlaGlu: 6.385 ± 0.067	3.87AlaPhe: 3.87 ± 0.046	8.665AlaGly: 8.665 ± 0.089	2.198AlaHis: 2.198 ± 0.035	5.212AlaIle: 5.212 ± 0.062	3.616AlaLys: 3.616 ± 0.059	13.545AlaLeu: 13.545 ± 0.111	2.917AlaMet: 2.917 ± 0.04	3.072AlaAsn: 3.072 ± 0.047	4.721AlaPro: 4.721 ± 0.068	5.318AlaGln: 5.318 ± 0.063	6.84AlaArg: 6.84 ± 0.065	6.262AlaSer: 6.262 ± 0.071	5.124AlaThr: 5.124 ± 0.069	7.356AlaVal: 7.356 ± 0.072	1.592AlaTrp: 1.592 ± 0.03	2.533AlaTyr: 2.533 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.023	0.13CysCys: 0.13 ± 0.009	0.547CysAsp: 0.547 ± 0.018	0.557CysGlu: 0.557 ± 0.019	0.36CysPhe: 0.36 ± 0.014	0.917CysGly: 0.917 ± 0.022	0.275CysHis: 0.275 ± 0.014	0.459CysIle: 0.459 ± 0.016	0.277CysLys: 0.277 ± 0.012	1.104CysLeu: 1.104 ± 0.023	0.224CysMet: 0.224 ± 0.012	0.276CysAsn: 0.276 ± 0.011	0.478CysPro: 0.478 ± 0.017	0.404CysGln: 0.404 ± 0.016	0.601CysArg: 0.601 ± 0.018	0.624CysSer: 0.624 ± 0.02	0.462CysThr: 0.462 ± 0.017	0.709CysVal: 0.709 ± 0.021	0.147CysTrp: 0.147 ± 0.009	0.252CysTyr: 0.252 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	5.581AspAla: 5.581 ± 0.059	0.543AspCys: 0.543 ± 0.018	2.975AspAsp: 2.975 ± 0.045	3.409AspGlu: 3.409 ± 0.048	2.091AspPhe: 2.091 ± 0.034	4.572AspGly: 4.572 ± 0.062	1.155AspHis: 1.155 ± 0.027	2.735AspIle: 2.735 ± 0.034	1.894AspLys: 1.894 ± 0.035	6.048AspLeu: 6.048 ± 0.063	1.207AspMet: 1.207 ± 0.024	1.667AspAsn: 1.667 ± 0.039	2.937AspPro: 2.937 ± 0.041	2.149AspGln: 2.149 ± 0.036	3.096AspArg: 3.096 ± 0.041	3.041AspSer: 3.041 ± 0.044	2.568AspThr: 2.568 ± 0.049	3.803AspVal: 3.803 ± 0.056	0.99AspTrp: 0.99 ± 0.025	1.757AspTyr: 1.757 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	6.241GluAla: 6.241 ± 0.061	0.407GluCys: 0.407 ± 0.015	2.447GluAsp: 2.447 ± 0.04	2.963GluGlu: 2.963 ± 0.048	1.803GluPhe: 1.803 ± 0.034	3.954GluGly: 3.954 ± 0.046	1.679GluHis: 1.679 ± 0.032	2.759GluIle: 2.759 ± 0.038	2.085GluLys: 2.085 ± 0.041	6.688GluLeu: 6.688 ± 0.07	1.287GluMet: 1.287 ± 0.028	1.613GluAsn: 1.613 ± 0.029	2.569GluPro: 2.569 ± 0.044	3.733GluGln: 3.733 ± 0.048	4.641GluArg: 4.641 ± 0.064	2.663GluSer: 2.663 ± 0.041	2.666GluThr: 2.666 ± 0.04	3.956GluVal: 3.956 ± 0.046	0.714GluTrp: 0.714 ± 0.02	1.221GluTyr: 1.221 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.024PheAla: 4.024 ± 0.051	0.469PheCys: 0.469 ± 0.017	2.492PheAsp: 2.492 ± 0.043	2.186PheGlu: 2.186 ± 0.037	1.482PhePhe: 1.482 ± 0.031	3.29PheGly: 3.29 ± 0.044	0.725PheHis: 0.725 ± 0.018	1.905PheIle: 1.905 ± 0.034	1.334PheLys: 1.334 ± 0.021	3.257PheLeu: 3.257 ± 0.049	0.837PheMet: 0.837 ± 0.018	1.452PheAsn: 1.452 ± 0.024	1.464PhePro: 1.464 ± 0.027	1.263PheGln: 1.263 ± 0.025	1.78PheArg: 1.78 ± 0.031	2.651PheSer: 2.651 ± 0.036	1.994PheThr: 1.994 ± 0.034	2.539PheVal: 2.539 ± 0.041	0.556PheTrp: 0.556 ± 0.018	1.029PheTyr: 1.029 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	7.382GlyAla: 7.382 ± 0.08	0.959GlyCys: 0.959 ± 0.025	3.919GlyAsp: 3.919 ± 0.046	4.573GlyGlu: 4.573 ± 0.048	3.442GlyPhe: 3.442 ± 0.045	6.049GlyGly: 6.049 ± 0.097	1.892GlyHis: 1.892 ± 0.036	4.073GlyIle: 4.073 ± 0.052	3.374GlyLys: 3.374 ± 0.047	9.352GlyLeu: 9.352 ± 0.096	2.187GlyMet: 2.187 ± 0.038	2.508GlyAsn: 2.508 ± 0.073	2.773GlyPro: 2.773 ± 0.043	3.814GlyGln: 3.814 ± 0.051	4.805GlyArg: 4.805 ± 0.056	4.696GlySer: 4.696 ± 0.06	3.92GlyThr: 3.92 ± 0.055	6.129GlyVal: 6.129 ± 0.062	1.34GlyTrp: 1.34 ± 0.03	2.44GlyTyr: 2.44 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.15HisAla: 2.15 ± 0.034	0.33HisCys: 0.33 ± 0.015	1.245HisAsp: 1.245 ± 0.028	1.173HisGlu: 1.173 ± 0.025	0.987HisPhe: 0.987 ± 0.025	2.005HisGly: 2.005 ± 0.032	0.616HisHis: 0.616 ± 0.02	1.0HisIle: 1.0 ± 0.024	0.663HisLys: 0.663 ± 0.016	2.623HisLeu: 2.623 ± 0.041	0.524HisMet: 0.524 ± 0.017	0.654HisAsn: 0.654 ± 0.019	1.369HisPro: 1.369 ± 0.025	0.93HisGln: 0.93 ± 0.025	1.349HisArg: 1.349 ± 0.028	1.316HisSer: 1.316 ± 0.03	1.001HisThr: 1.001 ± 0.024	1.384HisVal: 1.384 ± 0.027	0.446HisTrp: 0.446 ± 0.017	0.758HisTyr: 0.758 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.961IleAla: 5.961 ± 0.063	0.521IleCys: 0.521 ± 0.019	3.382IleAsp: 3.382 ± 0.045	3.413IleGlu: 3.413 ± 0.044	1.484IlePhe: 1.484 ± 0.035	4.57IleGly: 4.57 ± 0.055	0.995IleHis: 0.995 ± 0.023	2.125IleIle: 2.125 ± 0.037	1.767IleLys: 1.767 ± 0.027	4.171IleLeu: 4.171 ± 0.052	0.847IleMet: 0.847 ± 0.025	1.805IleAsn: 1.805 ± 0.038	2.204IlePro: 2.204 ± 0.037	1.636IleGln: 1.636 ± 0.029	2.819IleArg: 2.819 ± 0.038	3.024IleSer: 3.024 ± 0.044	2.66IleThr: 2.66 ± 0.051	3.333IleVal: 3.333 ± 0.046	0.504IleTrp: 0.504 ± 0.019	1.144IleTyr: 1.144 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	4.057LysAla: 4.057 ± 0.052	0.183LysCys: 0.183 ± 0.01	1.777LysAsp: 1.777 ± 0.029	1.656LysGlu: 1.656 ± 0.033	0.881LysPhe: 0.881 ± 0.026	2.636LysGly: 2.636 ± 0.04	0.765LysHis: 0.765 ± 0.022	1.652LysIle: 1.652 ± 0.032	1.324LysLys: 1.324 ± 0.034	3.759LysLeu: 3.759 ± 0.044	0.742LysMet: 0.742 ± 0.021	1.087LysAsn: 1.087 ± 0.025	2.143LysPro: 2.143 ± 0.047	1.549LysGln: 1.549 ± 0.029	2.317LysArg: 2.317 ± 0.037	1.807LysSer: 1.807 ± 0.031	2.002LysThr: 2.002 ± 0.038	2.757LysVal: 2.757 ± 0.038	0.362LysTrp: 0.362 ± 0.014	0.7LysTyr: 0.7 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.516LeuAla: 13.516 ± 0.125	1.236LeuCys: 1.236 ± 0.024	6.972LeuAsp: 6.972 ± 0.075	6.405LeuGlu: 6.405 ± 0.063	4.171LeuPhe: 4.171 ± 0.056	9.214LeuGly: 9.214 ± 0.081	2.454LeuHis: 2.454 ± 0.039	5.727LeuIle: 5.727 ± 0.06	4.415LeuLys: 4.415 ± 0.049	13.315LeuLeu: 13.315 ± 0.149	2.683LeuMet: 2.683 ± 0.038	3.828LeuAsn: 3.828 ± 0.057	6.188LeuPro: 6.188 ± 0.071	4.722LeuGln: 4.722 ± 0.055	7.125LeuArg: 7.125 ± 0.084	7.502LeuSer: 7.502 ± 0.072	6.0LeuThr: 6.0 ± 0.075	7.805LeuVal: 7.805 ± 0.064	1.375LeuTrp: 1.375 ± 0.032	2.524LeuTyr: 2.524 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	2.715MetAla: 2.715 ± 0.039	0.186MetCys: 0.186 ± 0.01	1.086MetAsp: 1.086 ± 0.026	0.972MetGlu: 0.972 ± 0.023	0.739MetPhe: 0.739 ± 0.02	1.735MetGly: 1.735 ± 0.031	0.493MetHis: 0.493 ± 0.016	1.172MetIle: 1.172 ± 0.026	0.864MetLys: 0.864 ± 0.021	2.678MetLeu: 2.678 ± 0.039	0.508MetMet: 0.508 ± 0.017	0.899MetAsn: 0.899 ± 0.022	1.354MetPro: 1.354 ± 0.028	1.028MetGln: 1.028 ± 0.022	1.456MetArg: 1.456 ± 0.03	1.752MetSer: 1.752 ± 0.031	1.514MetThr: 1.514 ± 0.031	1.563MetVal: 1.563 ± 0.028	0.189MetTrp: 0.189 ± 0.01	0.372MetTyr: 0.372 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.299AsnAla: 3.299 ± 0.05	0.291AsnCys: 0.291 ± 0.011	1.758AsnAsp: 1.758 ± 0.052	1.563AsnGlu: 1.563 ± 0.037	1.042AsnPhe: 1.042 ± 0.023	2.774AsnGly: 2.774 ± 0.066	0.661AsnHis: 0.661 ± 0.018	1.462AsnIle: 1.462 ± 0.028	0.956AsnLys: 0.956 ± 0.025	3.583AsnLeu: 3.583 ± 0.046	0.584AsnMet: 0.584 ± 0.019	1.029AsnAsn: 1.029 ± 0.04	2.034AsnPro: 2.034 ± 0.031	1.322AsnGln: 1.322 ± 0.03	1.95AsnArg: 1.95 ± 0.037	1.646AsnSer: 1.646 ± 0.04	1.63AsnThr: 1.63 ± 0.034	2.148AsnVal: 2.148 ± 0.044	0.469AsnTrp: 0.469 ± 0.017	0.818AsnTyr: 0.818 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	5.445ProAla: 5.445 ± 0.078	0.359ProCys: 0.359 ± 0.015	2.962ProAsp: 2.962 ± 0.044	3.176ProGlu: 3.176 ± 0.045	1.821ProPhe: 1.821 ± 0.03	3.949ProGly: 3.949 ± 0.048	1.061ProHis: 1.061 ± 0.025	2.078ProIle: 2.078 ± 0.04	1.636ProLys: 1.636 ± 0.032	5.687ProLeu: 5.687 ± 0.07	1.209ProMet: 1.209 ± 0.025	1.425ProAsn: 1.425 ± 0.03	1.986ProPro: 1.986 ± 0.04	2.354ProGln: 2.354 ± 0.038	2.535ProArg: 2.535 ± 0.041	2.689ProSer: 2.689 ± 0.045	2.508ProThr: 2.508 ± 0.046	3.951ProVal: 3.951 ± 0.053	0.784ProTrp: 0.784 ± 0.022	1.206ProTyr: 1.206 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	6.025GlnAla: 6.025 ± 0.067	0.329GlnCys: 0.329 ± 0.015	1.94GlnAsp: 1.94 ± 0.033	1.87GlnGlu: 1.87 ± 0.037	1.41GlnPhe: 1.41 ± 0.029	3.474GlnGly: 3.474 ± 0.043	1.118GlnHis: 1.118 ± 0.028	2.182GlnIle: 2.182 ± 0.032	1.261GlnLys: 1.261 ± 0.027	5.542GlnLeu: 5.542 ± 0.063	1.094GlnMet: 1.094 ± 0.025	1.163GlnAsn: 1.163 ± 0.027	2.413GlnPro: 2.413 ± 0.036	2.694GlnGln: 2.694 ± 0.046	3.59GlnArg: 3.59 ± 0.056	2.371GlnSer: 2.371 ± 0.033	2.195GlnThr: 2.195 ± 0.035	3.635GlnVal: 3.635 ± 0.044	0.76GlnTrp: 0.76 ± 0.022	0.99GlnTyr: 0.99 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	5.745ArgAla: 5.745 ± 0.064	0.627ArgCys: 0.627 ± 0.017	3.386ArgAsp: 3.386 ± 0.044	3.982ArgGlu: 3.982 ± 0.048	2.719ArgPhe: 2.719 ± 0.039	3.904ArgGly: 3.904 ± 0.044	1.668ArgHis: 1.668 ± 0.027	3.272ArgIle: 3.272 ± 0.04	2.045ArgLys: 2.045 ± 0.035	8.244ArgLeu: 8.244 ± 0.09	1.589ArgMet: 1.589 ± 0.029	1.94ArgAsn: 1.94 ± 0.034	2.733ArgPro: 2.733 ± 0.037	3.409ArgGln: 3.409 ± 0.052	4.137ArgArg: 4.137 ± 0.059	3.443ArgSer: 3.443 ± 0.046	2.794ArgThr: 2.794 ± 0.041	4.381ArgVal: 4.381 ± 0.057	1.02ArgTrp: 1.02 ± 0.027	2.065ArgTyr: 2.065 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.156SerAla: 6.156 ± 0.064	0.461SerCys: 0.461 ± 0.015	2.926SerAsp: 2.926 ± 0.049	3.107SerGlu: 3.107 ± 0.046	2.253SerPhe: 2.253 ± 0.032	5.343SerGly: 5.343 ± 0.068	1.288SerHis: 1.288 ± 0.028	2.906SerIle: 2.906 ± 0.041	1.873SerLys: 1.873 ± 0.038	7.153SerLeu: 7.153 ± 0.068	1.383SerMet: 1.383 ± 0.028	1.805SerAsn: 1.805 ± 0.039	2.799SerPro: 2.799 ± 0.038	2.527SerGln: 2.527 ± 0.041	3.658SerArg: 3.658 ± 0.049	3.583SerSer: 3.583 ± 0.055	3.093SerThr: 3.093 ± 0.049	4.198SerVal: 4.198 ± 0.045	0.75SerTrp: 0.75 ± 0.02	1.528SerTyr: 1.528 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	5.426ThrAla: 5.426 ± 0.078	0.493ThrCys: 0.493 ± 0.016	2.567ThrAsp: 2.567 ± 0.044	2.564ThrGlu: 2.564 ± 0.04	1.956ThrPhe: 1.956 ± 0.034	4.241ThrGly: 4.241 ± 0.06	1.124ThrHis: 1.124 ± 0.025	2.035ThrIle: 2.035 ± 0.068	1.153ThrLys: 1.153 ± 0.029	6.91ThrLeu: 6.91 ± 0.089	0.846ThrMet: 0.846 ± 0.023	1.268ThrAsn: 1.268 ± 0.032	3.272ThrPro: 3.272 ± 0.045	2.205ThrGln: 2.205 ± 0.034	3.092ThrArg: 3.092 ± 0.042	2.83ThrSer: 2.83 ± 0.049	2.627ThrThr: 2.627 ± 0.071	3.928ThrVal: 3.928 ± 0.077	0.743ThrTrp: 0.743 ± 0.021	1.291ThrTyr: 1.291 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	7.663ValAla: 7.663 ± 0.064	0.74ValCys: 0.74 ± 0.02	4.005ValAsp: 4.005 ± 0.051	4.33ValGlu: 4.33 ± 0.059	2.648ValPhe: 2.648 ± 0.043	5.344ValGly: 5.344 ± 0.059	1.43ValHis: 1.43 ± 0.027	3.944ValIle: 3.944 ± 0.054	2.366ValLys: 2.366 ± 0.035	8.254ValLeu: 8.254 ± 0.07	1.756ValMet: 1.756 ± 0.035	2.275ValAsn: 2.275 ± 0.05	3.413ValPro: 3.413 ± 0.045	2.854ValGln: 2.854 ± 0.042	4.278ValArg: 4.278 ± 0.053	4.528ValSer: 4.528 ± 0.053	3.869ValThr: 3.869 ± 0.086	5.454ValVal: 5.454 ± 0.072	0.89ValTrp: 0.89 ± 0.02	1.624ValTyr: 1.624 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.219TrpAla: 1.219 ± 0.028	0.16TrpCys: 0.16 ± 0.009	0.631TrpAsp: 0.631 ± 0.019	0.587TrpGlu: 0.587 ± 0.018	0.549TrpPhe: 0.549 ± 0.016	0.907TrpGly: 0.907 ± 0.023	0.368TrpHis: 0.368 ± 0.012	0.653TrpIle: 0.653 ± 0.02	0.47TrpLys: 0.47 ± 0.016	2.216TrpLeu: 2.216 ± 0.036	0.385TrpMet: 0.385 ± 0.014	0.481TrpAsn: 0.481 ± 0.015	0.667TrpPro: 0.667 ± 0.021	0.902TrpGln: 0.902 ± 0.021	1.064TrpArg: 1.064 ± 0.025	0.821TrpSer: 0.821 ± 0.022	0.648TrpThr: 0.648 ± 0.02	0.99TrpVal: 0.99 ± 0.025	0.24TrpTrp: 0.24 ± 0.013	0.342TrpTyr: 0.342 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.483TyrAla: 2.483 ± 0.037	0.294TyrCys: 0.294 ± 0.012	1.427TyrAsp: 1.427 ± 0.041	1.217TyrGlu: 1.217 ± 0.028	1.021TyrPhe: 1.021 ± 0.024	2.085TyrGly: 2.085 ± 0.034	0.557TyrHis: 0.557 ± 0.018	1.016TyrIle: 1.016 ± 0.024	0.782TyrLys: 0.782 ± 0.019	3.049TyrLeu: 3.049 ± 0.038	0.464TyrMet: 0.464 ± 0.015	0.771TyrAsn: 0.771 ± 0.02	1.305TyrPro: 1.305 ± 0.026	1.241TyrGln: 1.241 ± 0.031	1.97TyrArg: 1.97 ± 0.029	1.541TyrSer: 1.541 ± 0.031	1.296TyrThr: 1.296 ± 0.032	1.645TyrVal: 1.645 ± 0.029	0.428TyrTrp: 0.428 ± 0.012	0.702TyrTyr: 0.702 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5837 proteins (1970731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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