Proteome-pI 2.0 - Proteome Isoelectric Point Database

Mycetohabitans rhizoxinica (strain DSM 19002 / CIP 109453 / HKI 454) (Paraburkholderia rhizoxinica)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Mycetohabitans; Mycetohabitans rhizoxinica

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|E5AKW3|E5AKW3_MYCRK Type I secretion outer membrane protein OS=Mycetohabitans rhizoxinica (strain DSM 19002 / CIP 109453 / HKI 454) OX=882378 GN=RBRH_01295 PE=3 SV=1

MM1 pKa = 7.78EE2 pKa = 4.72YY3 pKa = 10.51KK4 pKa = 10.24SWMCLICGWIYY15 pKa = 11.01DD16 pKa = 4.19EE17 pKa = 4.49EE18 pKa = 6.25AGLPEE23 pKa = 4.41EE24 pKa = 5.23GIPPGTRR31 pKa = 11.84WEE33 pKa = 4.2DD34 pKa = 3.51VPINWTCPEE43 pKa = 3.97CGARR47 pKa = 11.84KK48 pKa = 9.54EE49 pKa = 4.07DD50 pKa = 3.87FEE52 pKa = 4.52MVQII56 pKa = 4.59

Molecular weight: 6.55 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|E5AKG4|E5AKG4_MYCRK tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC OS=Mycetohabitans rhizoxinica (strain DSM 19002 / CIP 109453 / HKI 454) OX=882378 GN=mnmC PE=3 SV=1

MM1 pKa = 7.38IMSARR6 pKa = 11.84LSKK9 pKa = 10.61RR10 pKa = 11.84MSVTVWHH17 pKa = 6.99AAHH20 pKa = 6.96ITRR23 pKa = 11.84IAQRR27 pKa = 11.84RR28 pKa = 11.84AARR31 pKa = 11.84SLRR34 pKa = 11.84HH35 pKa = 5.07HH36 pKa = 6.24VARR39 pKa = 11.84TRR41 pKa = 11.84ALTARR46 pKa = 11.84LSRR49 pKa = 11.84DD50 pKa = 2.73IAQRR54 pKa = 11.84WSGSVRR60 pKa = 11.84AWVHH64 pKa = 5.22SLVHH68 pKa = 5.89LRR70 pKa = 11.84LYY72 pKa = 10.25SIWRR76 pKa = 11.84ASLGRR81 pKa = 11.84SVRR84 pKa = 11.84VTPARR89 pKa = 11.84ALRR92 pKa = 11.84HH93 pKa = 5.45PRR95 pKa = 11.84QTPHH99 pKa = 6.21LRR101 pKa = 11.84LVRR104 pKa = 11.84TPRR107 pKa = 11.84RR108 pKa = 11.84HH109 pKa = 6.14APPSGPWSPYY119 pKa = 9.25FGGRR123 pKa = 3.27

Molecular weight: 14.25 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3859	0	3859	1108700	22	7658	287.3	31.45

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
12.334 ± 0.056	1.201 ± 0.015	5.426 ± 0.026	4.949 ± 0.035	3.322 ± 0.027	7.551 ± 0.046	2.63 ± 0.023	4.605 ± 0.029	3.06 ± 0.042	10.299 ± 0.061

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.384 ± 0.019	2.754 ± 0.023	5.344 ± 0.034	4.264 ± 0.042	7.759 ± 0.043	5.666 ± 0.034	5.274 ± 0.032	7.339 ± 0.037	1.382 ± 0.014	2.45 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski