bacterium 1xD42-87

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4468 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9A6R6|A0A3A9A6R6_9BACT Tetratricopeptide repeat protein OS=bacterium 1xD42-87 OX=2320113 GN=D7V90_10160 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.34RR3 pKa = 11.84KK4 pKa = 9.5ILAALLASTMVLSLVGCGGGSAEE27 pKa = 4.4TPAAPAADD35 pKa = 3.28SGAAEE40 pKa = 4.12EE41 pKa = 4.66APAAEE46 pKa = 4.48EE47 pKa = 4.38APAADD52 pKa = 4.3AEE54 pKa = 4.66TPAAAGGDD62 pKa = 3.51EE63 pKa = 4.99TLTVWCWDD71 pKa = 3.36PAFNMNAMYY80 pKa = 9.98EE81 pKa = 4.12AEE83 pKa = 4.55KK84 pKa = 10.62VYY86 pKa = 10.97QKK88 pKa = 10.91DD89 pKa = 3.39HH90 pKa = 7.2PNFKK94 pKa = 10.58LNVVEE99 pKa = 4.88TPWDD103 pKa = 4.66DD104 pKa = 3.25IQTKK108 pKa = 7.86VTTAATSGDD117 pKa = 4.31LSTLPDD123 pKa = 3.54IFLMQDD129 pKa = 2.48NAFQKK134 pKa = 10.78NVLAFPDD141 pKa = 3.4VCMDD145 pKa = 4.19LTNSGVDD152 pKa = 3.8FSQFAAGKK160 pKa = 7.15TAYY163 pKa = 10.46SVIDD167 pKa = 3.74GKK169 pKa = 10.87NYY171 pKa = 10.05GVPFDD176 pKa = 4.01NGAVVACYY184 pKa = 8.97RR185 pKa = 11.84TDD187 pKa = 3.71LLEE190 pKa = 4.12QAGLTIDD197 pKa = 4.75DD198 pKa = 4.12FTDD201 pKa = 3.1ITFDD205 pKa = 3.45QYY207 pKa = 11.91LEE209 pKa = 4.03NGKK212 pKa = 9.78KK213 pKa = 10.13VLEE216 pKa = 4.23ATGKK220 pKa = 9.73PLISMQAGEE229 pKa = 5.04CDD231 pKa = 4.95LIMMMLQSAGASLFNEE247 pKa = 4.84DD248 pKa = 2.64GTANIVGNDD257 pKa = 3.41TLKK260 pKa = 10.27TVMEE264 pKa = 4.72TYY266 pKa = 10.66KK267 pKa = 10.56SLKK270 pKa = 9.99DD271 pKa = 3.36AGVLTEE277 pKa = 3.97VNSWDD282 pKa = 3.86EE283 pKa = 4.02YY284 pKa = 11.1VGDD287 pKa = 4.0FVSGNVAGTINGCWIMASIQTAEE310 pKa = 4.17DD311 pKa = 3.53QSGNWAITNMPSLVGASGATNYY333 pKa = 10.65SNNGGSSWAVTTNCANPDD351 pKa = 3.82LACDD355 pKa = 4.28FLASTFAGSNEE366 pKa = 4.32LYY368 pKa = 11.08DD369 pKa = 4.55NILPSGALATWAPAGDD385 pKa = 3.41SSAYY389 pKa = 10.3GEE391 pKa = 4.12PNEE394 pKa = 4.56FFGGDD399 pKa = 3.22AVSAKK404 pKa = 10.18IVDD407 pKa = 4.67FATKK411 pKa = 10.37VPTNITGAYY420 pKa = 9.24YY421 pKa = 10.48YY422 pKa = 9.06EE423 pKa = 4.3ARR425 pKa = 11.84NAVATAITNYY435 pKa = 10.02INGSDD440 pKa = 5.09LEE442 pKa = 4.69TEE444 pKa = 4.29LQTAEE449 pKa = 3.66QTINFNMQQ457 pKa = 2.55

Molecular weight: 48.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9A2L3|A0A3A9A2L3_9BACT 30S ribosomal protein S2 OS=bacterium 1xD42-87 OX=2320113 GN=rpsB PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.72KK9 pKa = 7.48RR10 pKa = 11.84HH11 pKa = 5.01RR12 pKa = 11.84AKK14 pKa = 10.21VHH16 pKa = 5.29GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.14GGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4468

0

4468

1366805

17

2528

305.9

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.752 ± 0.039

1.555 ± 0.018

5.741 ± 0.031

7.968 ± 0.046

4.127 ± 0.028

7.163 ± 0.034

1.712 ± 0.017

6.823 ± 0.034

6.343 ± 0.032

8.906 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.137 ± 0.017

4.187 ± 0.024

3.163 ± 0.023

3.274 ± 0.019

5.047 ± 0.031

5.62 ± 0.025

5.175 ± 0.032

6.891 ± 0.034

1.002 ± 0.014

4.416 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski