Amino acid dipepetide frequency for bacterium 1xD42-87

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.163AlaAla: 8.163 ± 0.128	1.194AlaCys: 1.194 ± 0.034	5.093AlaAsp: 5.093 ± 0.067	6.048AlaGlu: 6.048 ± 0.08	3.259AlaPhe: 3.259 ± 0.062	6.75AlaGly: 6.75 ± 0.083	1.135AlaHis: 1.135 ± 0.027	4.264AlaIle: 4.264 ± 0.068	4.429AlaLys: 4.429 ± 0.06	7.259AlaLeu: 7.259 ± 0.079	2.235AlaMet: 2.235 ± 0.043	2.399AlaAsn: 2.399 ± 0.049	2.087AlaPro: 2.087 ± 0.044	2.515AlaGln: 2.515 ± 0.048	3.209AlaArg: 3.209 ± 0.051	3.784AlaSer: 3.784 ± 0.056	2.882AlaThr: 2.882 ± 0.051	6.891AlaVal: 6.891 ± 0.085	0.705AlaTrp: 0.705 ± 0.025	3.215AlaTyr: 3.215 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.098CysAla: 1.098 ± 0.032	0.271CysCys: 0.271 ± 0.015	0.885CysAsp: 0.885 ± 0.028	0.976CysGlu: 0.976 ± 0.026	0.762CysPhe: 0.762 ± 0.025	1.575CysGly: 1.575 ± 0.038	0.315CysHis: 0.315 ± 0.014	1.057CysIle: 1.057 ± 0.03	0.863CysLys: 0.863 ± 0.026	1.329CysLeu: 1.329 ± 0.035	0.488CysMet: 0.488 ± 0.019	0.578CysAsn: 0.578 ± 0.022	0.597CysPro: 0.597 ± 0.021	0.41CysGln: 0.41 ± 0.018	0.884CysArg: 0.884 ± 0.028	0.824CysSer: 0.824 ± 0.028	0.732CysThr: 0.732 ± 0.026	1.114CysVal: 1.114 ± 0.032	0.126CysTrp: 0.126 ± 0.011	0.667CysTyr: 0.667 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.304AspAla: 4.304 ± 0.08	0.876AspCys: 0.876 ± 0.025	2.959AspAsp: 2.959 ± 0.054	4.709AspGlu: 4.709 ± 0.062	2.718AspPhe: 2.718 ± 0.041	4.789AspGly: 4.789 ± 0.074	0.831AspHis: 0.831 ± 0.028	4.527AspIle: 4.527 ± 0.063	3.466AspLys: 3.466 ± 0.061	4.358AspLeu: 4.358 ± 0.056	2.013AspMet: 2.013 ± 0.044	2.294AspAsn: 2.294 ± 0.04	1.568AspPro: 1.568 ± 0.04	1.278AspGln: 1.278 ± 0.029	3.036AspArg: 3.036 ± 0.054	3.068AspSer: 3.068 ± 0.048	3.056AspThr: 3.056 ± 0.05	3.872AspVal: 3.872 ± 0.05	0.633AspTrp: 0.633 ± 0.023	3.052AspTyr: 3.052 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	5.899GluAla: 5.899 ± 0.073	0.864GluCys: 0.864 ± 0.031	4.352GluAsp: 4.352 ± 0.066	8.082GluGlu: 8.082 ± 0.094	2.458GluPhe: 2.458 ± 0.043	4.688GluGly: 4.688 ± 0.064	1.234GluHis: 1.234 ± 0.034	5.555GluIle: 5.555 ± 0.069	6.867GluLys: 6.867 ± 0.084	6.798GluLeu: 6.798 ± 0.078	2.55GluMet: 2.55 ± 0.041	4.309GluAsn: 4.309 ± 0.064	2.13GluPro: 2.13 ± 0.049	3.127GluGln: 3.127 ± 0.051	4.303GluArg: 4.303 ± 0.063	3.739GluSer: 3.739 ± 0.056	4.26GluThr: 4.26 ± 0.06	4.363GluVal: 4.363 ± 0.066	0.824GluTrp: 0.824 ± 0.029	3.276GluTyr: 3.276 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	3.106PheAla: 3.106 ± 0.051	0.827PheCys: 0.827 ± 0.025	2.613PheAsp: 2.613 ± 0.048	2.591PheGlu: 2.591 ± 0.046	2.023PhePhe: 2.023 ± 0.047	2.913PheGly: 2.913 ± 0.053	0.849PheHis: 0.849 ± 0.024	2.619PheIle: 2.619 ± 0.054	1.841PheLys: 1.841 ± 0.041	4.202PheLeu: 4.202 ± 0.062	1.178PheMet: 1.178 ± 0.03	1.398PheAsn: 1.398 ± 0.034	1.353PhePro: 1.353 ± 0.033	1.309PheGln: 1.309 ± 0.03	2.009PheArg: 2.009 ± 0.04	2.835PheSer: 2.835 ± 0.048	2.222PheThr: 2.222 ± 0.04	2.909PheVal: 2.909 ± 0.052	0.489PheTrp: 0.489 ± 0.019	1.982PheTyr: 1.982 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	5.086GlyAla: 5.086 ± 0.073	1.245GlyCys: 1.245 ± 0.027	3.655GlyAsp: 3.655 ± 0.06	5.415GlyGlu: 5.415 ± 0.069	3.088GlyPhe: 3.088 ± 0.055	5.113GlyGly: 5.113 ± 0.089	1.144GlyHis: 1.144 ± 0.032	5.639GlyIle: 5.639 ± 0.07	5.219GlyLys: 5.219 ± 0.061	5.65GlyLeu: 5.65 ± 0.077	2.657GlyMet: 2.657 ± 0.043	3.292GlyAsn: 3.292 ± 0.063	1.066GlyPro: 1.066 ± 0.036	2.324GlyGln: 2.324 ± 0.045	3.85GlyArg: 3.85 ± 0.061	4.094GlySer: 4.094 ± 0.061	4.034GlyThr: 4.034 ± 0.074	4.903GlyVal: 4.903 ± 0.051	0.736GlyTrp: 0.736 ± 0.025	3.417GlyTyr: 3.417 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.161HisAla: 1.161 ± 0.032	0.292HisCys: 0.292 ± 0.014	0.916HisAsp: 0.916 ± 0.027	1.123HisGlu: 1.123 ± 0.031	0.828HisPhe: 0.828 ± 0.027	1.283HisGly: 1.283 ± 0.033	0.372HisHis: 0.372 ± 0.017	1.421HisIle: 1.421 ± 0.034	0.979HisLys: 0.979 ± 0.028	1.449HisLeu: 1.449 ± 0.034	0.568HisMet: 0.568 ± 0.017	0.721HisAsn: 0.721 ± 0.025	0.778HisPro: 0.778 ± 0.026	0.443HisGln: 0.443 ± 0.018	0.882HisArg: 0.882 ± 0.027	0.918HisSer: 0.918 ± 0.03	0.922HisThr: 0.922 ± 0.028	1.058HisVal: 1.058 ± 0.029	0.18HisTrp: 0.18 ± 0.012	0.826HisTyr: 0.826 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.347IleAla: 5.347 ± 0.067	1.335IleCys: 1.335 ± 0.033	4.014IleAsp: 4.014 ± 0.059	4.77IleGlu: 4.77 ± 0.068	2.646IlePhe: 2.646 ± 0.049	4.599IleGly: 4.599 ± 0.062	1.248IleHis: 1.248 ± 0.03	4.238IleIle: 4.238 ± 0.068	3.657IleLys: 3.657 ± 0.065	6.58IleLeu: 6.58 ± 0.074	1.851IleMet: 1.851 ± 0.039	2.729IleAsn: 2.729 ± 0.05	2.902IlePro: 2.902 ± 0.047	2.003IleGln: 2.003 ± 0.04	3.804IleArg: 3.804 ± 0.06	4.562IleSer: 4.562 ± 0.06	3.767IleThr: 3.767 ± 0.059	4.64IleVal: 4.64 ± 0.054	0.671IleTrp: 0.671 ± 0.024	2.864IleTyr: 2.864 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	4.603LysAla: 4.603 ± 0.062	0.74LysCys: 0.74 ± 0.025	3.658LysAsp: 3.658 ± 0.051	6.127LysGlu: 6.127 ± 0.076	1.754LysPhe: 1.754 ± 0.034	3.848LysGly: 3.848 ± 0.055	0.969LysHis: 0.969 ± 0.025	4.399LysIle: 4.399 ± 0.061	5.345LysLys: 5.345 ± 0.073	4.971LysLeu: 4.971 ± 0.061	2.041LysMet: 2.041 ± 0.044	3.406LysAsn: 3.406 ± 0.048	1.932LysPro: 1.932 ± 0.039	2.274LysGln: 2.274 ± 0.04	3.408LysArg: 3.408 ± 0.054	3.235LysSer: 3.235 ± 0.055	3.403LysThr: 3.403 ± 0.063	3.834LysVal: 3.834 ± 0.063	0.656LysTrp: 0.656 ± 0.022	2.821LysTyr: 2.821 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	7.087LeuAla: 7.087 ± 0.087	1.596LeuCys: 1.596 ± 0.038	4.827LeuAsp: 4.827 ± 0.069	6.059LeuGlu: 6.059 ± 0.072	4.103LeuPhe: 4.103 ± 0.066	5.494LeuGly: 5.494 ± 0.071	1.62LeuHis: 1.62 ± 0.034	5.299LeuIle: 5.299 ± 0.069	5.427LeuLys: 5.427 ± 0.059	9.114LeuLeu: 9.114 ± 0.109	2.594LeuMet: 2.594 ± 0.046	3.496LeuAsn: 3.496 ± 0.053	3.411LeuPro: 3.411 ± 0.053	3.041LeuGln: 3.041 ± 0.054	4.274LeuArg: 4.274 ± 0.064	6.381LeuSer: 6.381 ± 0.079	5.059LeuThr: 5.059 ± 0.064	5.553LeuVal: 5.553 ± 0.076	0.897LeuTrp: 0.897 ± 0.027	3.723LeuTyr: 3.723 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	2.514MetAla: 2.514 ± 0.041	0.367MetCys: 0.367 ± 0.018	1.901MetAsp: 1.901 ± 0.036	2.867MetGlu: 2.867 ± 0.05	1.037MetPhe: 1.037 ± 0.027	2.131MetGly: 2.131 ± 0.039	0.477MetHis: 0.477 ± 0.018	1.994MetIle: 1.994 ± 0.039	2.343MetLys: 2.343 ± 0.04	2.679MetLeu: 2.679 ± 0.046	0.917MetMet: 0.917 ± 0.032	1.439MetAsn: 1.439 ± 0.031	1.212MetPro: 1.212 ± 0.032	1.147MetGln: 1.147 ± 0.033	1.583MetArg: 1.583 ± 0.035	1.815MetSer: 1.815 ± 0.035	1.792MetThr: 1.792 ± 0.041	1.986MetVal: 1.986 ± 0.04	0.244MetTrp: 0.244 ± 0.013	0.926MetTyr: 0.926 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.206AsnAla: 3.206 ± 0.051	0.637AsnCys: 0.637 ± 0.024	2.133AsnAsp: 2.133 ± 0.038	2.839AsnGlu: 2.839 ± 0.05	1.539AsnPhe: 1.539 ± 0.033	3.513AsnGly: 3.513 ± 0.058	0.776AsnHis: 0.776 ± 0.028	3.197AsnIle: 3.197 ± 0.054	2.232AsnLys: 2.232 ± 0.047	3.603AsnLeu: 3.603 ± 0.052	1.424AsnMet: 1.424 ± 0.033	1.773AsnAsn: 1.773 ± 0.043	1.79AsnPro: 1.79 ± 0.037	1.299AsnGln: 1.299 ± 0.031	2.377AsnArg: 2.377 ± 0.048	2.191AsnSer: 2.191 ± 0.046	2.229AsnThr: 2.229 ± 0.045	2.788AsnVal: 2.788 ± 0.051	0.426AsnTrp: 0.426 ± 0.02	1.899AsnTyr: 1.899 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	2.6ProAla: 2.6 ± 0.046	0.443ProCys: 0.443 ± 0.019	2.254ProAsp: 2.254 ± 0.046	3.085ProGlu: 3.085 ± 0.049	1.481ProPhe: 1.481 ± 0.034	2.242ProGly: 2.242 ± 0.043	0.553ProHis: 0.553 ± 0.018	1.907ProIle: 1.907 ± 0.035	1.737ProLys: 1.737 ± 0.036	2.715ProLeu: 2.715 ± 0.04	0.889ProMet: 0.889 ± 0.028	1.141ProAsn: 1.141 ± 0.028	0.786ProPro: 0.786 ± 0.027	1.125ProGln: 1.125 ± 0.032	1.053ProArg: 1.053 ± 0.035	1.539ProSer: 1.539 ± 0.032	1.435ProThr: 1.435 ± 0.036	2.824ProVal: 2.824 ± 0.048	0.293ProTrp: 0.293 ± 0.016	1.526ProTyr: 1.526 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.565GlnAla: 2.565 ± 0.041	0.368GlnCys: 0.368 ± 0.018	1.644GlnAsp: 1.644 ± 0.036	2.873GlnGlu: 2.873 ± 0.05	1.212GlnPhe: 1.212 ± 0.03	2.144GlnGly: 2.144 ± 0.043	0.456GlnHis: 0.456 ± 0.019	2.701GlnIle: 2.701 ± 0.048	2.491GlnLys: 2.491 ± 0.043	2.575GlnLeu: 2.575 ± 0.043	1.265GlnMet: 1.265 ± 0.031	1.582GlnAsn: 1.582 ± 0.033	0.943GlnPro: 0.943 ± 0.029	1.15GlnGln: 1.15 ± 0.04	1.521GlnArg: 1.521 ± 0.035	1.667GlnSer: 1.667 ± 0.04	1.877GlnThr: 1.877 ± 0.038	1.923GlnVal: 1.923 ± 0.037	0.334GlnTrp: 0.334 ± 0.015	1.452GlnTyr: 1.452 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	3.314ArgAla: 3.314 ± 0.05	0.691ArgCys: 0.691 ± 0.022	2.662ArgAsp: 2.662 ± 0.041	4.865ArgGlu: 4.865 ± 0.074	2.065ArgPhe: 2.065 ± 0.042	2.893ArgGly: 2.893 ± 0.048	0.962ArgHis: 0.962 ± 0.027	3.89ArgIle: 3.89 ± 0.05	3.82ArgLys: 3.82 ± 0.057	4.455ArgLeu: 4.455 ± 0.063	1.822ArgMet: 1.822 ± 0.033	2.188ArgAsn: 2.188 ± 0.041	1.281ArgPro: 1.281 ± 0.033	2.108ArgGln: 2.108 ± 0.04	2.978ArgArg: 2.978 ± 0.058	2.269ArgSer: 2.269 ± 0.041	2.367ArgThr: 2.367 ± 0.045	3.099ArgVal: 3.099 ± 0.053	0.459ArgTrp: 0.459 ± 0.02	2.281ArgTyr: 2.281 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.464SerAla: 4.464 ± 0.068	0.879SerCys: 0.879 ± 0.024	3.286SerAsp: 3.286 ± 0.053	3.986SerGlu: 3.986 ± 0.064	2.699SerPhe: 2.699 ± 0.049	4.928SerGly: 4.928 ± 0.059	1.054SerHis: 1.054 ± 0.027	3.669SerIle: 3.669 ± 0.06	2.854SerLys: 2.854 ± 0.047	5.124SerLeu: 5.124 ± 0.072	1.76SerMet: 1.76 ± 0.044	2.014SerAsn: 2.014 ± 0.042	1.686SerPro: 1.686 ± 0.036	1.7SerGln: 1.7 ± 0.038	2.861SerArg: 2.861 ± 0.048	3.078SerSer: 3.078 ± 0.053	2.526SerThr: 2.526 ± 0.051	4.54SerVal: 4.54 ± 0.053	0.525SerTrp: 0.525 ± 0.023	2.568SerTyr: 2.568 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.572ThrAla: 4.572 ± 0.076	0.649ThrCys: 0.649 ± 0.024	3.39ThrAsp: 3.39 ± 0.061	4.105ThrGlu: 4.105 ± 0.055	2.083ThrPhe: 2.083 ± 0.042	4.446ThrGly: 4.446 ± 0.069	0.848ThrHis: 0.848 ± 0.026	3.396ThrIle: 3.396 ± 0.059	2.722ThrLys: 2.722 ± 0.043	4.656ThrLeu: 4.656 ± 0.06	1.402ThrMet: 1.402 ± 0.031	1.845ThrAsn: 1.845 ± 0.042	2.016ThrPro: 2.016 ± 0.043	1.465ThrGln: 1.465 ± 0.034	1.961ThrArg: 1.961 ± 0.042	2.495ThrSer: 2.495 ± 0.05	2.456ThrThr: 2.456 ± 0.051	4.53ThrVal: 4.53 ± 0.069	0.482ThrTrp: 0.482 ± 0.019	2.226ThrTyr: 2.226 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	4.73ValAla: 4.73 ± 0.062	1.385ValCys: 1.385 ± 0.035	3.792ValAsp: 3.792 ± 0.052	4.978ValGlu: 4.978 ± 0.07	3.085ValPhe: 3.085 ± 0.057	4.331ValGly: 4.331 ± 0.059	1.072ValHis: 1.072 ± 0.028	4.907ValIle: 4.907 ± 0.059	4.157ValLys: 4.157 ± 0.062	6.412ValLeu: 6.412 ± 0.075	2.164ValMet: 2.164 ± 0.038	2.864ValAsn: 2.864 ± 0.05	2.457ValPro: 2.457 ± 0.041	1.983ValGln: 1.983 ± 0.039	3.357ValArg: 3.357 ± 0.059	4.78ValSer: 4.78 ± 0.059	4.042ValThr: 4.042 ± 0.06	4.719ValVal: 4.719 ± 0.064	0.754ValTrp: 0.754 ± 0.024	2.943ValTyr: 2.943 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.608TrpAla: 0.608 ± 0.022	0.195TrpCys: 0.195 ± 0.012	0.575TrpAsp: 0.575 ± 0.019	0.827TrpGlu: 0.827 ± 0.024	0.449TrpPhe: 0.449 ± 0.019	0.735TrpGly: 0.735 ± 0.026	0.23TrpHis: 0.23 ± 0.012	0.661TrpIle: 0.661 ± 0.02	0.736TrpLys: 0.736 ± 0.023	1.013TrpLeu: 1.013 ± 0.03	0.306TrpMet: 0.306 ± 0.014	0.547TrpAsn: 0.547 ± 0.021	0.149TrpPro: 0.149 ± 0.012	0.46TrpGln: 0.46 ± 0.02	0.519TrpArg: 0.519 ± 0.021	0.478TrpSer: 0.478 ± 0.019	0.427TrpThr: 0.427 ± 0.021	0.544TrpVal: 0.544 ± 0.022	0.14TrpTrp: 0.14 ± 0.009	0.416TrpTyr: 0.416 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.088TyrAla: 3.088 ± 0.046	0.695TyrCys: 0.695 ± 0.024	2.798TyrAsp: 2.798 ± 0.068	3.354TyrGlu: 3.354 ± 0.055	1.979TyrPhe: 1.979 ± 0.042	3.211TyrGly: 3.211 ± 0.053	0.976TyrHis: 0.976 ± 0.027	2.886TyrIle: 2.886 ± 0.048	2.241TyrLys: 2.241 ± 0.045	4.113TyrLeu: 4.113 ± 0.067	1.245TyrMet: 1.245 ± 0.028	1.856TyrAsn: 1.856 ± 0.035	1.488TyrPro: 1.488 ± 0.034	1.581TyrGln: 1.581 ± 0.036	2.598TyrArg: 2.598 ± 0.044	2.446TyrSer: 2.446 ± 0.044	2.259TyrThr: 2.259 ± 0.051	2.826TyrVal: 2.826 ± 0.044	0.44TyrTrp: 0.44 ± 0.019	2.081TyrTyr: 2.081 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4468 proteins (1366805 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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