Amino acid dipepetide frequency for Methylomusa anaerophila

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.949AlaAla: 10.949 ± 0.16	1.031AlaCys: 1.031 ± 0.032	4.822AlaAsp: 4.822 ± 0.07	5.7AlaGlu: 5.7 ± 0.079	3.067AlaPhe: 3.067 ± 0.056	8.704AlaGly: 8.704 ± 0.172	1.346AlaHis: 1.346 ± 0.034	6.192AlaIle: 6.192 ± 0.081	5.083AlaLys: 5.083 ± 0.068	8.123AlaLeu: 8.123 ± 0.087	2.316AlaMet: 2.316 ± 0.044	3.43AlaAsn: 3.43 ± 0.063	2.603AlaPro: 2.603 ± 0.053	3.187AlaGln: 3.187 ± 0.053	4.064AlaArg: 4.064 ± 0.066	4.355AlaSer: 4.355 ± 0.073	4.022AlaThr: 4.022 ± 0.065	7.638AlaVal: 7.638 ± 0.085	0.781AlaTrp: 0.781 ± 0.026	2.67AlaTyr: 2.67 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.848CysAla: 0.848 ± 0.029	0.242CysCys: 0.242 ± 0.016	0.602CysAsp: 0.602 ± 0.022	0.652CysGlu: 0.652 ± 0.025	0.47CysPhe: 0.47 ± 0.018	1.202CysGly: 1.202 ± 0.037	0.299CysHis: 0.299 ± 0.014	0.754CysIle: 0.754 ± 0.028	0.547CysLys: 0.547 ± 0.023	1.132CysLeu: 1.132 ± 0.033	0.304CysMet: 0.304 ± 0.015	0.447CysAsn: 0.447 ± 0.018	0.639CysPro: 0.639 ± 0.026	0.45CysGln: 0.45 ± 0.019	0.72CysArg: 0.72 ± 0.024	0.784CysSer: 0.784 ± 0.029	0.523CysThr: 0.523 ± 0.023	0.746CysVal: 0.746 ± 0.026	0.134CysTrp: 0.134 ± 0.011	0.441CysTyr: 0.441 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.941AspAla: 3.941 ± 0.064	0.708AspCys: 0.708 ± 0.024	2.291AspAsp: 2.291 ± 0.049	3.117AspGlu: 3.117 ± 0.06	2.189AspPhe: 2.189 ± 0.04	3.644AspGly: 3.644 ± 0.063	0.912AspHis: 0.912 ± 0.03	4.614AspIle: 4.614 ± 0.077	3.286AspLys: 3.286 ± 0.048	4.825AspLeu: 4.825 ± 0.07	1.308AspMet: 1.308 ± 0.033	2.289AspAsn: 2.289 ± 0.082	2.103AspPro: 2.103 ± 0.047	1.662AspGln: 1.662 ± 0.04	2.481AspArg: 2.481 ± 0.044	2.78AspSer: 2.78 ± 0.047	2.745AspThr: 2.745 ± 0.084	3.484AspVal: 3.484 ± 0.064	0.673AspTrp: 0.673 ± 0.023	2.061AspTyr: 2.061 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.335GluAla: 5.335 ± 0.076	0.603GluCys: 0.603 ± 0.018	2.654GluAsp: 2.654 ± 0.05	4.405GluGlu: 4.405 ± 0.078	2.339GluPhe: 2.339 ± 0.045	3.489GluGly: 3.489 ± 0.059	1.121GluHis: 1.121 ± 0.029	4.958GluIle: 4.958 ± 0.073	4.602GluLys: 4.602 ± 0.074	6.359GluLeu: 6.359 ± 0.081	1.754GluMet: 1.754 ± 0.037	2.946GluAsn: 2.946 ± 0.062	2.02GluPro: 2.02 ± 0.044	2.957GluGln: 2.957 ± 0.06	3.317GluArg: 3.317 ± 0.062	2.766GluSer: 2.766 ± 0.052	3.248GluThr: 3.248 ± 0.049	4.004GluVal: 4.004 ± 0.061	0.646GluTrp: 0.646 ± 0.022	2.325GluTyr: 2.325 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.348PheAla: 3.348 ± 0.058	0.529PheCys: 0.529 ± 0.022	2.223PheAsp: 2.223 ± 0.038	1.927PheGlu: 1.927 ± 0.042	1.856PhePhe: 1.856 ± 0.041	3.149PheGly: 3.149 ± 0.054	0.729PheHis: 0.729 ± 0.023	2.836PheIle: 2.836 ± 0.054	1.823PheLys: 1.823 ± 0.044	3.784PheLeu: 3.784 ± 0.066	0.943PheMet: 0.943 ± 0.025	1.677PheAsn: 1.677 ± 0.044	1.467PhePro: 1.467 ± 0.038	1.271PheGln: 1.271 ± 0.028	1.697PheArg: 1.697 ± 0.038	2.843PheSer: 2.843 ± 0.049	2.296PheThr: 2.296 ± 0.043	2.454PheVal: 2.454 ± 0.047	0.503PheTrp: 0.503 ± 0.022	1.371PheTyr: 1.371 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	5.851GlyAla: 5.851 ± 0.102	1.069GlyCys: 1.069 ± 0.03	3.703GlyAsp: 3.703 ± 0.083	4.311GlyGlu: 4.311 ± 0.058	3.054GlyPhe: 3.054 ± 0.05	5.86GlyGly: 5.86 ± 0.13	1.408GlyHis: 1.408 ± 0.032	6.2GlyIle: 6.2 ± 0.078	4.873GlyLys: 4.873 ± 0.055	7.291GlyLeu: 7.291 ± 0.098	2.181GlyMet: 2.181 ± 0.04	3.308GlyAsn: 3.308 ± 0.126	2.152GlyPro: 2.152 ± 0.103	3.106GlyGln: 3.106 ± 0.078	3.773GlyArg: 3.773 ± 0.069	4.423GlySer: 4.423 ± 0.09	4.379GlyThr: 4.379 ± 0.108	5.482GlyVal: 5.482 ± 0.075	0.839GlyTrp: 0.839 ± 0.026	2.835GlyTyr: 2.835 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.289HisAla: 1.289 ± 0.03	0.327HisCys: 0.327 ± 0.017	0.926HisAsp: 0.926 ± 0.028	0.932HisGlu: 0.932 ± 0.031	0.712HisPhe: 0.712 ± 0.024	1.428HisGly: 1.428 ± 0.035	0.44HisHis: 0.44 ± 0.019	1.412HisIle: 1.412 ± 0.029	0.861HisLys: 0.861 ± 0.025	1.786HisLeu: 1.786 ± 0.04	0.445HisMet: 0.445 ± 0.02	0.809HisAsn: 0.809 ± 0.025	1.035HisPro: 1.035 ± 0.028	0.697HisGln: 0.697 ± 0.023	0.916HisArg: 0.916 ± 0.026	1.124HisSer: 1.124 ± 0.031	1.013HisThr: 1.013 ± 0.03	1.132HisVal: 1.132 ± 0.028	0.233HisTrp: 0.233 ± 0.013	0.714HisTyr: 0.714 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.823IleAla: 6.823 ± 0.069	0.883IleCys: 0.883 ± 0.031	4.192IleAsp: 4.192 ± 0.064	4.332IleGlu: 4.332 ± 0.067	2.725IlePhe: 2.725 ± 0.051	5.536IleGly: 5.536 ± 0.086	1.409IleHis: 1.409 ± 0.033	5.439IleIle: 5.439 ± 0.074	4.126IleLys: 4.126 ± 0.061	6.728IleLeu: 6.728 ± 0.093	1.717IleMet: 1.717 ± 0.041	3.449IleAsn: 3.449 ± 0.071	3.334IlePro: 3.334 ± 0.054	2.364IleGln: 2.364 ± 0.044	3.547IleArg: 3.547 ± 0.064	4.596IleSer: 4.596 ± 0.064	4.28IleThr: 4.28 ± 0.069	5.151IleVal: 5.151 ± 0.072	0.611IleTrp: 0.611 ± 0.026	2.151IleTyr: 2.151 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.724LysAla: 4.724 ± 0.072	0.557LysCys: 0.557 ± 0.021	2.993LysAsp: 2.993 ± 0.056	4.182LysGlu: 4.182 ± 0.077	1.747LysPhe: 1.747 ± 0.039	3.424LysGly: 3.424 ± 0.051	0.952LysHis: 0.952 ± 0.027	4.441LysIle: 4.441 ± 0.071	3.64LysLys: 3.64 ± 0.069	5.484LysLeu: 5.484 ± 0.067	1.524LysMet: 1.524 ± 0.038	2.813LysAsn: 2.813 ± 0.053	2.333LysPro: 2.333 ± 0.044	2.434LysGln: 2.434 ± 0.046	2.651LysArg: 2.651 ± 0.047	2.866LysSer: 2.866 ± 0.049	3.361LysThr: 3.361 ± 0.058	4.042LysVal: 4.042 ± 0.061	0.546LysTrp: 0.546 ± 0.02	2.078LysTyr: 2.078 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	10.253LeuAla: 10.253 ± 0.104	1.104LeuCys: 1.104 ± 0.026	5.0LeuAsp: 5.0 ± 0.062	5.766LeuGlu: 5.766 ± 0.078	3.78LeuPhe: 3.78 ± 0.066	7.036LeuGly: 7.036 ± 0.094	1.729LeuHis: 1.729 ± 0.043	6.175LeuIle: 6.175 ± 0.079	5.686LeuLys: 5.686 ± 0.073	9.858LeuLeu: 9.858 ± 0.122	2.171LeuMet: 2.171 ± 0.046	4.213LeuAsn: 4.213 ± 0.061	4.557LeuPro: 4.557 ± 0.068	3.546LeuGln: 3.546 ± 0.075	4.563LeuArg: 4.563 ± 0.074	6.37LeuSer: 6.37 ± 0.076	6.159LeuThr: 6.159 ± 0.106	6.372LeuVal: 6.372 ± 0.076	0.89LeuTrp: 0.89 ± 0.025	3.009LeuTyr: 3.009 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.471MetAla: 2.471 ± 0.042	0.214MetCys: 0.214 ± 0.013	1.388MetAsp: 1.388 ± 0.031	1.645MetGlu: 1.645 ± 0.032	0.794MetPhe: 0.794 ± 0.024	1.749MetGly: 1.749 ± 0.038	0.41MetHis: 0.41 ± 0.019	1.631MetIle: 1.631 ± 0.034	1.503MetLys: 1.503 ± 0.035	2.555MetLeu: 2.555 ± 0.047	0.608MetMet: 0.608 ± 0.021	1.129MetAsn: 1.129 ± 0.03	1.142MetPro: 1.142 ± 0.033	0.949MetGln: 0.949 ± 0.028	1.227MetArg: 1.227 ± 0.032	1.493MetSer: 1.493 ± 0.039	1.392MetThr: 1.392 ± 0.033	1.721MetVal: 1.721 ± 0.04	0.202MetTrp: 0.202 ± 0.013	0.647MetTyr: 0.647 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.077AsnAla: 3.077 ± 0.059	0.521AsnCys: 0.521 ± 0.019	1.961AsnAsp: 1.961 ± 0.057	2.255AsnGlu: 2.255 ± 0.05	1.733AsnPhe: 1.733 ± 0.035	3.037AsnGly: 3.037 ± 0.069	0.83AsnHis: 0.83 ± 0.027	3.798AsnIle: 3.798 ± 0.068	2.463AsnLys: 2.463 ± 0.045	4.275AsnLeu: 4.275 ± 0.078	1.095AsnMet: 1.095 ± 0.03	2.188AsnAsn: 2.188 ± 0.08	2.347AsnPro: 2.347 ± 0.042	1.65AsnGln: 1.65 ± 0.051	2.108AsnArg: 2.108 ± 0.036	2.582AsnSer: 2.582 ± 0.065	2.464AsnThr: 2.464 ± 0.062	2.813AsnVal: 2.813 ± 0.074	0.53AsnTrp: 0.53 ± 0.02	1.58AsnTyr: 1.58 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	4.256ProAla: 4.256 ± 0.071	0.447ProCys: 0.447 ± 0.021	2.558ProAsp: 2.558 ± 0.052	3.089ProGlu: 3.089 ± 0.059	1.675ProPhe: 1.675 ± 0.042	3.407ProGly: 3.407 ± 0.048	0.768ProHis: 0.768 ± 0.025	2.274ProIle: 2.274 ± 0.041	1.769ProLys: 1.769 ± 0.041	3.844ProLeu: 3.844 ± 0.068	0.774ProMet: 0.774 ± 0.023	1.529ProAsn: 1.529 ± 0.038	1.527ProPro: 1.527 ± 0.037	1.632ProGln: 1.632 ± 0.038	1.65ProArg: 1.65 ± 0.042	2.007ProSer: 2.007 ± 0.05	1.925ProThr: 1.925 ± 0.102	3.765ProVal: 3.765 ± 0.055	0.441ProTrp: 0.441 ± 0.018	1.296ProTyr: 1.296 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.033GlnAla: 4.033 ± 0.09	0.355GlnCys: 0.355 ± 0.018	1.885GlnAsp: 1.885 ± 0.04	2.822GlnGlu: 2.822 ± 0.059	1.299GlnPhe: 1.299 ± 0.029	2.721GlnGly: 2.721 ± 0.06	0.706GlnHis: 0.706 ± 0.024	2.641GlnIle: 2.641 ± 0.05	2.178GlnLys: 2.178 ± 0.044	3.633GlnLeu: 3.633 ± 0.061	1.01GlnMet: 1.01 ± 0.032	1.644GlnAsn: 1.644 ± 0.043	1.563GlnPro: 1.563 ± 0.037	1.892GlnGln: 1.892 ± 0.05	1.905GlnArg: 1.905 ± 0.039	2.038GlnSer: 2.038 ± 0.045	2.118GlnThr: 2.118 ± 0.047	2.767GlnVal: 2.767 ± 0.05	0.379GlnTrp: 0.379 ± 0.016	1.354GlnTyr: 1.354 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	3.156ArgAla: 3.156 ± 0.054	0.575ArgCys: 0.575 ± 0.025	2.428ArgAsp: 2.428 ± 0.045	3.86ArgGlu: 3.86 ± 0.072	1.936ArgPhe: 1.936 ± 0.038	2.9ArgGly: 2.9 ± 0.048	1.051ArgHis: 1.051 ± 0.031	3.659ArgIle: 3.659 ± 0.061	2.716ArgLys: 2.716 ± 0.049	5.357ArgLeu: 5.357 ± 0.076	1.36ArgMet: 1.36 ± 0.032	2.006ArgAsn: 2.006 ± 0.042	1.856ArgPro: 1.856 ± 0.041	2.696ArgGln: 2.696 ± 0.053	2.863ArgArg: 2.863 ± 0.069	2.424ArgSer: 2.424 ± 0.041	2.37ArgThr: 2.37 ± 0.048	3.049ArgVal: 3.049 ± 0.057	0.563ArgTrp: 0.563 ± 0.023	1.852ArgTyr: 1.852 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.46SerAla: 4.46 ± 0.084	0.665SerCys: 0.665 ± 0.023	2.604SerAsp: 2.604 ± 0.048	3.038SerGlu: 3.038 ± 0.056	2.503SerPhe: 2.503 ± 0.051	5.116SerGly: 5.116 ± 0.095	1.034SerHis: 1.034 ± 0.028	4.107SerIle: 4.107 ± 0.067	2.64SerLys: 2.64 ± 0.051	6.2SerLeu: 6.2 ± 0.083	1.423SerMet: 1.423 ± 0.03	2.187SerAsn: 2.187 ± 0.044	2.597SerPro: 2.597 ± 0.05	2.336SerGln: 2.336 ± 0.046	2.933SerArg: 2.933 ± 0.055	3.58SerSer: 3.58 ± 0.073	2.799SerThr: 2.799 ± 0.056	3.925SerVal: 3.925 ± 0.056	0.614SerTrp: 0.614 ± 0.022	2.032SerTyr: 2.032 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	5.539ThrAla: 5.539 ± 0.095	0.589ThrCys: 0.589 ± 0.024	2.787ThrAsp: 2.787 ± 0.059	2.96ThrGlu: 2.96 ± 0.052	2.012ThrPhe: 2.012 ± 0.047	5.629ThrGly: 5.629 ± 0.142	0.963ThrHis: 0.963 ± 0.03	3.902ThrIle: 3.902 ± 0.069	2.575ThrLys: 2.575 ± 0.045	5.316ThrLeu: 5.316 ± 0.086	1.215ThrMet: 1.215 ± 0.038	2.061ThrAsn: 2.061 ± 0.058	2.567ThrPro: 2.567 ± 0.055	1.672ThrGln: 1.672 ± 0.036	2.263ThrArg: 2.263 ± 0.04	2.716ThrSer: 2.716 ± 0.052	2.931ThrThr: 2.931 ± 0.078	4.646ThrVal: 4.646 ± 0.087	0.484ThrTrp: 0.484 ± 0.019	1.716ThrTyr: 1.716 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	6.477ValAla: 6.477 ± 0.085	0.883ValCys: 0.883 ± 0.028	3.703ValAsp: 3.703 ± 0.065	4.287ValGlu: 4.287 ± 0.066	2.925ValPhe: 2.925 ± 0.052	4.739ValGly: 4.739 ± 0.071	1.163ValHis: 1.163 ± 0.029	5.338ValIle: 5.338 ± 0.072	4.213ValLys: 4.213 ± 0.059	6.999ValLeu: 6.999 ± 0.093	1.775ValMet: 1.775 ± 0.036	3.244ValAsn: 3.244 ± 0.063	2.857ValPro: 2.857 ± 0.044	2.313ValGln: 2.313 ± 0.039	3.405ValArg: 3.405 ± 0.057	4.382ValSer: 4.382 ± 0.063	4.182ValThr: 4.182 ± 0.087	5.4ValVal: 5.4 ± 0.088	0.706ValTrp: 0.706 ± 0.024	2.288ValTyr: 2.288 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.737TrpAla: 0.737 ± 0.023	0.121TrpCys: 0.121 ± 0.01	0.559TrpAsp: 0.559 ± 0.024	0.678TrpGlu: 0.678 ± 0.025	0.412TrpPhe: 0.412 ± 0.02	0.746TrpGly: 0.746 ± 0.025	0.224TrpHis: 0.224 ± 0.016	0.594TrpIle: 0.594 ± 0.021	0.552TrpLys: 0.552 ± 0.029	1.236TrpLeu: 1.236 ± 0.038	0.244TrpMet: 0.244 ± 0.014	0.458TrpAsn: 0.458 ± 0.02	0.386TrpPro: 0.386 ± 0.016	0.641TrpGln: 0.641 ± 0.021	0.624TrpArg: 0.624 ± 0.025	0.57TrpSer: 0.57 ± 0.023	0.469TrpThr: 0.469 ± 0.018	0.686TrpVal: 0.686 ± 0.025	0.134TrpTrp: 0.134 ± 0.012	0.319TrpTyr: 0.319 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.513TyrAla: 2.513 ± 0.035	0.515TyrCys: 0.515 ± 0.021	1.832TyrAsp: 1.832 ± 0.043	1.891TyrGlu: 1.891 ± 0.037	1.498TyrPhe: 1.498 ± 0.039	2.583TyrGly: 2.583 ± 0.045	0.736TyrHis: 0.736 ± 0.026	2.428TyrIle: 2.428 ± 0.049	1.655TyrLys: 1.655 ± 0.042	3.45TyrLeu: 3.45 ± 0.057	0.702TyrMet: 0.702 ± 0.023	1.478TyrAsn: 1.478 ± 0.041	1.55TyrPro: 1.55 ± 0.036	1.49TyrGln: 1.49 ± 0.036	1.971TyrArg: 1.971 ± 0.044	2.096TyrSer: 2.096 ± 0.042	1.868TyrThr: 1.868 ± 0.045	2.003TyrVal: 2.003 ± 0.044	0.479TyrTrp: 0.479 ± 0.019	1.356TyrTyr: 1.356 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4152 proteins (1354701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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