Granulicella sibirica

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Granulicella

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q0SYY0|A0A4Q0SYY0_9BACT Cell division protein FtsA OS=Granulicella sibirica OX=2479048 GN=ftsA PE=3 SV=1
MM1 pKa = 7.22ATVGLNFGSATSGTGFDD18 pKa = 3.26VSTTVSSILAIQGAIEE34 pKa = 4.36TPWKK38 pKa = 10.12NQLATLQAQDD48 pKa = 3.65TVLTTLGTDD57 pKa = 3.83LSTLTTSIQSLTDD70 pKa = 3.03ITGVLASKK78 pKa = 10.38QGSSSNTNVVALASADD94 pKa = 3.41ATATAGSHH102 pKa = 3.94TVVVNSLATTASEE115 pKa = 4.16YY116 pKa = 11.08SDD118 pKa = 4.95LVTDD122 pKa = 3.99SSAVLSGSITIAGTTITIGSSNNTLASLAASINAADD158 pKa = 4.33LGVNASVITDD168 pKa = 3.5STGSRR173 pKa = 11.84LSLVSSTGGSAGQSLISGISGSLTYY198 pKa = 7.78TTTSNTTPATLDD210 pKa = 3.57LNAGQTGVNASLTVDD225 pKa = 3.92GVNISSATNTITGAIPGVTFQALATGSSQIAIANDD260 pKa = 3.2NSSVEE265 pKa = 4.35SAVSSFVTAYY275 pKa = 10.52NAVIKK280 pKa = 10.12DD281 pKa = 3.42INGQEE286 pKa = 4.35GNDD289 pKa = 3.35ATGAAEE295 pKa = 4.19PLYY298 pKa = 10.65GSPTLATVQSQLQSALSGGGGSGSITSIAQLGLSVNNDD336 pKa = 2.72GTLTLNNATLDD347 pKa = 3.69TTLNTSYY354 pKa = 11.43SDD356 pKa = 3.55VAGFLQNSGSFGQTVSSALNNLGTQAPDD384 pKa = 3.14GLVYY388 pKa = 10.79LAQQQNTSEE397 pKa = 4.2EE398 pKa = 4.06ASLNASITKK407 pKa = 9.88EE408 pKa = 3.94DD409 pKa = 4.11AVLATQKK416 pKa = 10.49ISLTNEE422 pKa = 3.72LNTANQILQSIPSQLNEE439 pKa = 3.61INEE442 pKa = 4.29IYY444 pKa = 10.65SAVTGYY450 pKa = 11.22NNQNGG455 pKa = 3.52

Molecular weight: 45.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q0T739|A0A4Q0T739_9BACT Histidine kinase OS=Granulicella sibirica OX=2479048 GN=GRAN_0796 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.69RR13 pKa = 11.84AKK15 pKa = 9.55THH17 pKa = 5.47GFLTRR22 pKa = 11.84MKK24 pKa = 9.03TKK26 pKa = 10.57AGAAVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5287

0

5287

1767618

37

4944

334.3

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.888 ± 0.04

0.829 ± 0.012

5.158 ± 0.028

5.346 ± 0.045

3.919 ± 0.02

8.172 ± 0.037

2.245 ± 0.017

4.978 ± 0.024

3.674 ± 0.031

9.93 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.021

3.268 ± 0.032

5.411 ± 0.028

3.689 ± 0.024

6.016 ± 0.045

6.505 ± 0.037

6.49 ± 0.068

7.249 ± 0.033

1.303 ± 0.015

2.675 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski