Amino acid dipepetide frequency for Granulicella sibirica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.309AlaAla: 13.309 ± 0.141	0.921AlaCys: 0.921 ± 0.024	5.211AlaAsp: 5.211 ± 0.052	6.108AlaGlu: 6.108 ± 0.084	3.887AlaPhe: 3.887 ± 0.054	9.125AlaGly: 9.125 ± 0.083	2.176AlaHis: 2.176 ± 0.04	5.635AlaIle: 5.635 ± 0.065	4.172AlaLys: 4.172 ± 0.067	10.733AlaLeu: 10.733 ± 0.106	2.838AlaMet: 2.838 ± 0.051	3.474AlaAsn: 3.474 ± 0.057	5.274AlaPro: 5.274 ± 0.069	4.155AlaGln: 4.155 ± 0.067	6.147AlaArg: 6.147 ± 0.065	7.172AlaSer: 7.172 ± 0.078	7.017AlaThr: 7.017 ± 0.116	7.652AlaVal: 7.652 ± 0.079	1.315AlaTrp: 1.315 ± 0.028	2.561AlaTyr: 2.561 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	0.829CysAla: 0.829 ± 0.022	0.124CysCys: 0.124 ± 0.009	0.441CysAsp: 0.441 ± 0.018	0.421CysGlu: 0.421 ± 0.016	0.355CysPhe: 0.355 ± 0.015	0.865CysGly: 0.865 ± 0.025	0.252CysHis: 0.252 ± 0.014	0.41CysIle: 0.41 ± 0.014	0.222CysLys: 0.222 ± 0.011	0.799CysLeu: 0.799 ± 0.024	0.193CysMet: 0.193 ± 0.01	0.235CysAsn: 0.235 ± 0.013	0.424CysPro: 0.424 ± 0.019	0.22CysGln: 0.22 ± 0.012	0.479CysArg: 0.479 ± 0.017	0.567CysSer: 0.567 ± 0.017	0.5CysThr: 0.5 ± 0.02	0.609CysVal: 0.609 ± 0.024	0.113CysTrp: 0.113 ± 0.008	0.23CysTyr: 0.23 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	5.679AspAla: 5.679 ± 0.06	0.406AspCys: 0.406 ± 0.019	2.528AspAsp: 2.528 ± 0.04	2.951AspGlu: 2.951 ± 0.05	2.196AspPhe: 2.196 ± 0.035	4.692AspGly: 4.692 ± 0.069	1.259AspHis: 1.259 ± 0.031	2.322AspIle: 2.322 ± 0.038	1.735AspLys: 1.735 ± 0.036	5.4AspLeu: 5.4 ± 0.062	0.961AspMet: 0.961 ± 0.022	1.353AspAsn: 1.353 ± 0.027	3.395AspPro: 3.395 ± 0.05	1.787AspGln: 1.787 ± 0.04	3.267AspArg: 3.267 ± 0.051	2.827AspSer: 2.827 ± 0.047	2.746AspThr: 2.746 ± 0.044	3.729AspVal: 3.729 ± 0.047	0.806AspTrp: 0.806 ± 0.025	1.545AspTyr: 1.545 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	6.163GluAla: 6.163 ± 0.078	0.364GluCys: 0.364 ± 0.014	2.664GluAsp: 2.664 ± 0.045	3.185GluGlu: 3.185 ± 0.057	1.911GluPhe: 1.911 ± 0.036	3.76GluGly: 3.76 ± 0.052	1.358GluHis: 1.358 ± 0.03	3.053GluIle: 3.053 ± 0.051	2.359GluLys: 2.359 ± 0.046	5.174GluLeu: 5.174 ± 0.072	1.439GluMet: 1.439 ± 0.032	1.561GluAsn: 1.561 ± 0.029	2.325GluPro: 2.325 ± 0.042	2.297GluGln: 2.297 ± 0.044	3.892GluArg: 3.892 ± 0.063	3.006GluSer: 3.006 ± 0.049	3.156GluThr: 3.156 ± 0.047	3.825GluVal: 3.825 ± 0.057	0.663GluTrp: 0.663 ± 0.02	1.309GluTyr: 1.309 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.202PheAla: 4.202 ± 0.055	0.434PheCys: 0.434 ± 0.016	2.447PheAsp: 2.447 ± 0.038	2.021PheGlu: 2.021 ± 0.035	1.703PhePhe: 1.703 ± 0.038	3.583PheGly: 3.583 ± 0.054	1.027PheHis: 1.027 ± 0.024	1.453PheIle: 1.453 ± 0.028	1.103PheLys: 1.103 ± 0.026	3.909PheLeu: 3.909 ± 0.062	0.681PheMet: 0.681 ± 0.021	1.342PheAsn: 1.342 ± 0.036	1.875PhePro: 1.875 ± 0.034	1.325PheGln: 1.325 ± 0.026	2.245PheArg: 2.245 ± 0.04	2.784PheSer: 2.784 ± 0.042	2.541PheThr: 2.541 ± 0.052	2.775PheVal: 2.775 ± 0.047	0.54PheTrp: 0.54 ± 0.02	1.201PheTyr: 1.201 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	7.743GlyAla: 7.743 ± 0.088	0.772GlyCys: 0.772 ± 0.025	4.073GlyAsp: 4.073 ± 0.057	3.917GlyGlu: 3.917 ± 0.057	3.495GlyPhe: 3.495 ± 0.051	6.722GlyGly: 6.722 ± 0.09	1.827GlyHis: 1.827 ± 0.037	4.451GlyIle: 4.451 ± 0.06	3.709GlyLys: 3.709 ± 0.061	7.693GlyLeu: 7.693 ± 0.083	2.045GlyMet: 2.045 ± 0.041	2.734GlyAsn: 2.734 ± 0.057	3.365GlyPro: 3.365 ± 0.048	2.907GlyGln: 2.907 ± 0.046	4.743GlyArg: 4.743 ± 0.057	5.708GlySer: 5.708 ± 0.074	5.915GlyThr: 5.915 ± 0.125	6.126GlyVal: 6.126 ± 0.06	1.283GlyTrp: 1.283 ± 0.03	2.497GlyTyr: 2.497 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.245HisAla: 2.245 ± 0.039	0.205HisCys: 0.205 ± 0.012	1.211HisAsp: 1.211 ± 0.024	1.16HisGlu: 1.16 ± 0.026	1.04HisPhe: 1.04 ± 0.028	1.948HisGly: 1.948 ± 0.039	0.61HisHis: 0.61 ± 0.019	1.107HisIle: 1.107 ± 0.028	0.622HisLys: 0.622 ± 0.02	2.432HisLeu: 2.432 ± 0.04	0.494HisMet: 0.494 ± 0.019	0.634HisAsn: 0.634 ± 0.019	1.513HisPro: 1.513 ± 0.033	0.748HisGln: 0.748 ± 0.019	1.337HisArg: 1.337 ± 0.031	1.292HisSer: 1.292 ± 0.027	1.287HisThr: 1.287 ± 0.027	1.568HisVal: 1.568 ± 0.03	0.375HisTrp: 0.375 ± 0.014	0.618HisTyr: 0.618 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.233IleAla: 6.233 ± 0.065	0.418IleCys: 0.418 ± 0.014	3.042IleAsp: 3.042 ± 0.043	2.911IleGlu: 2.911 ± 0.051	1.833IlePhe: 1.833 ± 0.036	4.278IleGly: 4.278 ± 0.052	1.116IleHis: 1.116 ± 0.027	1.823IleIle: 1.823 ± 0.039	1.415IleLys: 1.415 ± 0.031	4.653IleLeu: 4.653 ± 0.058	0.737IleMet: 0.737 ± 0.023	1.61IleAsn: 1.61 ± 0.034	2.938IlePro: 2.938 ± 0.043	1.673IleGln: 1.673 ± 0.031	3.03IleArg: 3.03 ± 0.047	3.299IleSer: 3.299 ± 0.046	3.25IleThr: 3.25 ± 0.068	3.702IleVal: 3.702 ± 0.049	0.538IleTrp: 0.538 ± 0.019	1.281IleTyr: 1.281 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.999LysAla: 3.999 ± 0.055	0.183LysCys: 0.183 ± 0.011	1.991LysAsp: 1.991 ± 0.04	1.931LysGlu: 1.931 ± 0.041	1.134LysPhe: 1.134 ± 0.024	2.599LysGly: 2.599 ± 0.041	0.794LysHis: 0.794 ± 0.022	1.905LysIle: 1.905 ± 0.031	1.713LysLys: 1.713 ± 0.044	3.674LysLeu: 3.674 ± 0.047	1.019LysMet: 1.019 ± 0.024	1.175LysAsn: 1.175 ± 0.029	2.284LysPro: 2.284 ± 0.039	1.488LysGln: 1.488 ± 0.03	2.19LysArg: 2.19 ± 0.042	2.144LysSer: 2.144 ± 0.037	2.492LysThr: 2.492 ± 0.038	2.686LysVal: 2.686 ± 0.043	0.389LysTrp: 0.389 ± 0.017	0.946LysTyr: 0.946 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	11.397LeuAla: 11.397 ± 0.105	0.91LeuCys: 0.91 ± 0.025	5.136LeuAsp: 5.136 ± 0.058	5.279LeuGlu: 5.279 ± 0.073	3.664LeuPhe: 3.664 ± 0.063	7.74LeuGly: 7.74 ± 0.078	2.272LeuHis: 2.272 ± 0.036	4.629LeuIle: 4.629 ± 0.071	3.714LeuLys: 3.714 ± 0.053	10.101LeuLeu: 10.101 ± 0.109	2.026LeuMet: 2.026 ± 0.037	3.229LeuAsn: 3.229 ± 0.049	5.568LeuPro: 5.568 ± 0.06	3.441LeuGln: 3.441 ± 0.05	6.5LeuArg: 6.5 ± 0.09	6.659LeuSer: 6.659 ± 0.072	6.671LeuThr: 6.671 ± 0.082	6.604LeuVal: 6.604 ± 0.069	1.26LeuTrp: 1.26 ± 0.036	2.503LeuTyr: 2.503 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.461MetAla: 2.461 ± 0.042	0.147MetCys: 0.147 ± 0.009	1.061MetAsp: 1.061 ± 0.027	1.16MetGlu: 1.16 ± 0.027	0.661MetPhe: 0.661 ± 0.019	1.549MetGly: 1.549 ± 0.034	0.549MetHis: 0.549 ± 0.018	1.089MetIle: 1.089 ± 0.029	1.017MetLys: 1.017 ± 0.027	2.315MetLeu: 2.315 ± 0.044	0.57MetMet: 0.57 ± 0.019	0.773MetAsn: 0.773 ± 0.018	1.385MetPro: 1.385 ± 0.029	0.952MetGln: 0.952 ± 0.026	1.636MetArg: 1.636 ± 0.037	1.482MetSer: 1.482 ± 0.031	1.521MetThr: 1.521 ± 0.03	1.559MetVal: 1.559 ± 0.033	0.208MetTrp: 0.208 ± 0.012	0.446MetTyr: 0.446 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.452AsnAla: 3.452 ± 0.047	0.284AsnCys: 0.284 ± 0.013	1.62AsnAsp: 1.62 ± 0.037	1.494AsnGlu: 1.494 ± 0.028	1.352AsnPhe: 1.352 ± 0.034	3.044AsnGly: 3.044 ± 0.054	0.718AsnHis: 0.718 ± 0.019	1.597AsnIle: 1.597 ± 0.036	0.903AsnLys: 0.903 ± 0.025	3.261AsnLeu: 3.261 ± 0.045	0.569AsnMet: 0.569 ± 0.017	1.183AsnAsn: 1.183 ± 0.035	2.33AsnPro: 2.33 ± 0.044	1.28AsnGln: 1.28 ± 0.032	1.795AsnArg: 1.795 ± 0.036	1.962AsnSer: 1.962 ± 0.041	1.995AsnThr: 1.995 ± 0.052	2.309AsnVal: 2.309 ± 0.045	0.421AsnTrp: 0.421 ± 0.016	1.108AsnTyr: 1.108 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	6.186ProAla: 6.186 ± 0.074	0.344ProCys: 0.344 ± 0.016	3.23ProAsp: 3.23 ± 0.05	3.592ProGlu: 3.592 ± 0.05	1.989ProPhe: 1.989 ± 0.036	4.75ProGly: 4.75 ± 0.064	1.157ProHis: 1.157 ± 0.027	2.6ProIle: 2.6 ± 0.045	1.988ProLys: 1.988 ± 0.034	4.717ProLeu: 4.717 ± 0.053	1.165ProMet: 1.165 ± 0.027	1.987ProAsn: 1.987 ± 0.042	2.637ProPro: 2.637 ± 0.064	1.943ProGln: 1.943 ± 0.04	2.505ProArg: 2.505 ± 0.041	3.59ProSer: 3.59 ± 0.051	3.466ProThr: 3.466 ± 0.053	4.216ProVal: 4.216 ± 0.055	0.691ProTrp: 0.691 ± 0.021	1.353ProTyr: 1.353 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.029GlnAla: 4.029 ± 0.056	0.222GlnCys: 0.222 ± 0.012	1.63GlnAsp: 1.63 ± 0.026	1.706GlnGlu: 1.706 ± 0.035	1.404GlnPhe: 1.404 ± 0.032	2.61GlnGly: 2.61 ± 0.039	0.832GlnHis: 0.832 ± 0.021	2.088GlnIle: 2.088 ± 0.034	1.44GlnLys: 1.44 ± 0.03	3.346GlnLeu: 3.346 ± 0.046	1.01GlnMet: 1.01 ± 0.027	1.271GlnAsn: 1.271 ± 0.028	2.088GlnPro: 2.088 ± 0.038	1.884GlnGln: 1.884 ± 0.05	2.383GlnArg: 2.383 ± 0.042	2.31GlnSer: 2.31 ± 0.041	2.538GlnThr: 2.538 ± 0.049	2.681GlnVal: 2.681 ± 0.043	0.45GlnTrp: 0.45 ± 0.017	0.965GlnTyr: 0.965 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	5.703ArgAla: 5.703 ± 0.066	0.461ArgCys: 0.461 ± 0.018	3.117ArgAsp: 3.117 ± 0.05	3.681ArgGlu: 3.681 ± 0.058	2.632ArgPhe: 2.632 ± 0.045	3.888ArgGly: 3.888 ± 0.059	1.353ArgHis: 1.353 ± 0.034	3.399ArgIle: 3.399 ± 0.045	2.439ArgLys: 2.439 ± 0.043	6.168ArgLeu: 6.168 ± 0.069	1.725ArgMet: 1.725 ± 0.037	1.989ArgAsn: 1.989 ± 0.037	2.826ArgPro: 2.826 ± 0.051	2.255ArgGln: 2.255 ± 0.034	4.165ArgArg: 4.165 ± 0.059	3.753ArgSer: 3.753 ± 0.05	3.495ArgThr: 3.495 ± 0.045	4.357ArgVal: 4.357 ± 0.062	0.955ArgTrp: 0.955 ± 0.027	1.8ArgTyr: 1.8 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.764SerAla: 6.764 ± 0.069	0.548SerCys: 0.548 ± 0.02	3.047SerAsp: 3.047 ± 0.047	3.039SerGlu: 3.039 ± 0.046	2.79SerPhe: 2.79 ± 0.048	6.229SerGly: 6.229 ± 0.077	1.315SerHis: 1.315 ± 0.029	3.454SerIle: 3.454 ± 0.051	2.194SerLys: 2.194 ± 0.04	6.573SerLeu: 6.573 ± 0.065	1.406SerMet: 1.406 ± 0.031	2.142SerAsn: 2.142 ± 0.045	3.654SerPro: 3.654 ± 0.055	2.192SerGln: 2.192 ± 0.038	3.551SerArg: 3.551 ± 0.05	4.649SerSer: 4.649 ± 0.073	4.258SerThr: 4.258 ± 0.07	4.698SerVal: 4.698 ± 0.062	0.78SerTrp: 0.78 ± 0.022	1.765SerTyr: 1.765 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	6.887ThrAla: 6.887 ± 0.103	0.519ThrCys: 0.519 ± 0.019	3.023ThrAsp: 3.023 ± 0.05	2.899ThrGlu: 2.899 ± 0.045	2.665ThrPhe: 2.665 ± 0.057	5.834ThrGly: 5.834 ± 0.099	1.34ThrHis: 1.34 ± 0.03	3.452ThrIle: 3.452 ± 0.059	2.014ThrLys: 2.014 ± 0.036	6.781ThrLeu: 6.781 ± 0.087	1.262ThrMet: 1.262 ± 0.025	2.132ThrAsn: 2.132 ± 0.051	4.389ThrPro: 4.389 ± 0.072	2.227ThrGln: 2.227 ± 0.045	3.219ThrArg: 3.219 ± 0.052	4.161ThrSer: 4.161 ± 0.074	4.406ThrThr: 4.406 ± 0.115	5.169ThrVal: 5.169 ± 0.094	0.79ThrTrp: 0.79 ± 0.026	1.735ThrTyr: 1.735 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	7.928ValAla: 7.928 ± 0.076	0.678ValCys: 0.678 ± 0.023	3.965ValAsp: 3.965 ± 0.055	4.062ValGlu: 4.062 ± 0.057	2.735ValPhe: 2.735 ± 0.044	5.248ValGly: 5.248 ± 0.062	1.537ValHis: 1.537 ± 0.031	3.523ValIle: 3.523 ± 0.051	2.487ValLys: 2.487 ± 0.043	7.422ValLeu: 7.422 ± 0.083	1.529ValMet: 1.529 ± 0.032	2.34ValAsn: 2.34 ± 0.043	3.888ValPro: 3.888 ± 0.058	2.457ValGln: 2.457 ± 0.037	4.401ValArg: 4.401 ± 0.059	4.931ValSer: 4.931 ± 0.069	5.08ValThr: 5.08 ± 0.092	5.626ValVal: 5.626 ± 0.068	0.868ValTrp: 0.868 ± 0.024	1.788ValTyr: 1.788 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.07TrpAla: 1.07 ± 0.031	0.118TrpCys: 0.118 ± 0.009	0.642TrpAsp: 0.642 ± 0.02	0.549TrpGlu: 0.549 ± 0.018	0.554TrpPhe: 0.554 ± 0.018	0.889TrpGly: 0.889 ± 0.026	0.375TrpHis: 0.375 ± 0.014	0.734TrpIle: 0.734 ± 0.024	0.601TrpLys: 0.601 ± 0.02	1.397TrpLeu: 1.397 ± 0.031	0.404TrpMet: 0.404 ± 0.017	0.58TrpAsn: 0.58 ± 0.017	0.535TrpPro: 0.535 ± 0.02	0.598TrpGln: 0.598 ± 0.022	0.848TrpArg: 0.848 ± 0.024	0.928TrpSer: 0.928 ± 0.026	0.818TrpThr: 0.818 ± 0.026	0.819TrpVal: 0.819 ± 0.025	0.22TrpTrp: 0.22 ± 0.013	0.355TrpTyr: 0.355 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.606TyrAla: 2.606 ± 0.038	0.229TyrCys: 0.229 ± 0.011	1.508TyrAsp: 1.508 ± 0.029	1.397TyrGlu: 1.397 ± 0.032	1.191TyrPhe: 1.191 ± 0.028	2.371TyrGly: 2.371 ± 0.045	0.579TyrHis: 0.579 ± 0.018	1.056TyrIle: 1.056 ± 0.023	0.888TyrLys: 0.888 ± 0.024	2.753TyrLeu: 2.753 ± 0.043	0.468TyrMet: 0.468 ± 0.015	0.928TyrAsn: 0.928 ± 0.03	1.416TyrPro: 1.416 ± 0.033	1.055TyrGln: 1.055 ± 0.026	1.827TyrArg: 1.827 ± 0.036	1.824TyrSer: 1.824 ± 0.039	1.753TyrThr: 1.753 ± 0.042	1.782TyrVal: 1.782 ± 0.031	0.372TyrTrp: 0.372 ± 0.014	0.746TyrTyr: 0.746 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5287 proteins (1767618 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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