TM7 phylum sp. oral taxon 346
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 930 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563D6X2|A0A563D6X2_9BACT Ribonuclease 3 OS=TM7 phylum sp. oral taxon 346 OX=713049 GN=rnc PE=3 SV=1
MM1 pKa = 7.48 NYY3 pKa = 10.16 DD4 pKa = 4.37 RR5 pKa = 11.84 YY6 pKa = 11.16 LEE8 pKa = 4.23 LQTRR12 pKa = 11.84 LEE14 pKa = 4.2 WFYY17 pKa = 11.49 DD18 pKa = 3.78 FHH20 pKa = 8.64 PEE22 pKa = 3.95 FFDD25 pKa = 6.68 DD26 pKa = 5.7 IPPEE30 pKa = 4.17 QKK32 pKa = 10.59 KK33 pKa = 10.28 LLQDD37 pKa = 3.46 TFLYY41 pKa = 10.11 DD42 pKa = 4.39 APDD45 pKa = 3.25 EE46 pKa = 4.96 GYY48 pKa = 9.79 PEE50 pKa = 4.37 SLQDD54 pKa = 5.48 FYY56 pKa = 11.86 DD57 pKa = 3.55 DD58 pKa = 4.62 TINGKK63 pKa = 8.04 PTLQHH68 pKa = 6.7 DD69 pKa = 3.83 ALLAVDD75 pKa = 5.12 ALYY78 pKa = 10.47 QAAGAGSLFADD89 pKa = 3.33 NEE91 pKa = 4.14 YY92 pKa = 10.92 RR93 pKa = 11.84 SLADD97 pKa = 3.2
Molecular weight: 11.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.605
ProMoST 3.948
Dawson 3.834
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A563CUM8|A0A563CUM8_9BACT Exodeoxyribonuclease 7 small subunit OS=TM7 phylum sp. oral taxon 346 OX=713049 GN=xseB PE=3 SV=1
MM1 pKa = 7.86 PKK3 pKa = 9.64 MKK5 pKa = 8.79 THH7 pKa = 6.94 KK8 pKa = 9.72 GTAKK12 pKa = 10.41 RR13 pKa = 11.84 IKK15 pKa = 9.53 LTSTGKK21 pKa = 8.2 LTRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 AFGNHH31 pKa = 5.04 MLSKK35 pKa = 10.15 KK36 pKa = 10.17 SKK38 pKa = 8.95 SRR40 pKa = 11.84 KK41 pKa = 8.93 RR42 pKa = 11.84 NINTSAIIKK51 pKa = 10.37 GGIAKK56 pKa = 9.67 NVKK59 pKa = 9.67 RR60 pKa = 11.84 ALGVV64 pKa = 3.24
Molecular weight: 7.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.472
IPC2_protein 11.096
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.778
Grimsley 12.852
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.501
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
930
0
930
283914
38
1710
305.3
33.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.122 ± 0.1
0.697 ± 0.024
5.959 ± 0.065
5.835 ± 0.085
3.55 ± 0.053
6.632 ± 0.07
1.827 ± 0.031
6.979 ± 0.069
6.161 ± 0.086
9.194 ± 0.098
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.04
4.366 ± 0.068
3.904 ± 0.062
4.101 ± 0.055
5.738 ± 0.077
6.601 ± 0.077
5.625 ± 0.061
7.017 ± 0.074
1.051 ± 0.029
3.357 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here