TM7 phylum sp. oral taxon 346

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A563D6X2|A0A563D6X2_9BACT Ribonuclease 3 OS=TM7 phylum sp. oral taxon 346 OX=713049 GN=rnc PE=3 SV=1
MM1 pKa = 7.48NYY3 pKa = 10.16DD4 pKa = 4.37RR5 pKa = 11.84YY6 pKa = 11.16LEE8 pKa = 4.23LQTRR12 pKa = 11.84LEE14 pKa = 4.2WFYY17 pKa = 11.49DD18 pKa = 3.78FHH20 pKa = 8.64PEE22 pKa = 3.95FFDD25 pKa = 6.68DD26 pKa = 5.7IPPEE30 pKa = 4.17QKK32 pKa = 10.59KK33 pKa = 10.28LLQDD37 pKa = 3.46TFLYY41 pKa = 10.11DD42 pKa = 4.39APDD45 pKa = 3.25EE46 pKa = 4.96GYY48 pKa = 9.79PEE50 pKa = 4.37SLQDD54 pKa = 5.48FYY56 pKa = 11.86DD57 pKa = 3.55DD58 pKa = 4.62TINGKK63 pKa = 8.04PTLQHH68 pKa = 6.7DD69 pKa = 3.83ALLAVDD75 pKa = 5.12ALYY78 pKa = 10.47QAAGAGSLFADD89 pKa = 3.33NEE91 pKa = 4.14YY92 pKa = 10.92RR93 pKa = 11.84SLADD97 pKa = 3.2

Molecular weight:
11.34 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A563CUM8|A0A563CUM8_9BACT Exodeoxyribonuclease 7 small subunit OS=TM7 phylum sp. oral taxon 346 OX=713049 GN=xseB PE=3 SV=1
MM1 pKa = 7.86PKK3 pKa = 9.64MKK5 pKa = 8.79THH7 pKa = 6.94KK8 pKa = 9.72GTAKK12 pKa = 10.41RR13 pKa = 11.84IKK15 pKa = 9.53LTSTGKK21 pKa = 8.2LTRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84AFGNHH31 pKa = 5.04MLSKK35 pKa = 10.15KK36 pKa = 10.17SKK38 pKa = 8.95SRR40 pKa = 11.84KK41 pKa = 8.93RR42 pKa = 11.84NINTSAIIKK51 pKa = 10.37GGIAKK56 pKa = 9.67NVKK59 pKa = 9.67RR60 pKa = 11.84ALGVV64 pKa = 3.24

Molecular weight:
7.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

930

0

930

283914

38

1710

305.3

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.122 ± 0.1

0.697 ± 0.024

5.959 ± 0.065

5.835 ± 0.085

3.55 ± 0.053

6.632 ± 0.07

1.827 ± 0.031

6.979 ± 0.069

6.161 ± 0.086

9.194 ± 0.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.04

4.366 ± 0.068

3.904 ± 0.062

4.101 ± 0.055

5.738 ± 0.077

6.601 ± 0.077

5.625 ± 0.061

7.017 ± 0.074

1.051 ± 0.029

3.357 ± 0.045

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski