Amino acid dipepetide frequency for Streptomyces sp. SID10115

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.255AlaAla: 20.255 ± 0.131	1.122AlaCys: 1.122 ± 0.023	8.545AlaAsp: 8.545 ± 0.062	8.672AlaGlu: 8.672 ± 0.071	3.501AlaPhe: 3.501 ± 0.04	12.285AlaGly: 12.285 ± 0.081	2.956AlaHis: 2.956 ± 0.045	3.397AlaIle: 3.397 ± 0.043	3.181AlaLys: 3.181 ± 0.048	14.062AlaLeu: 14.062 ± 0.087	2.594AlaMet: 2.594 ± 0.028	1.877AlaAsn: 1.877 ± 0.03	6.935AlaPro: 6.935 ± 0.066	3.61AlaGln: 3.61 ± 0.039	9.997AlaArg: 9.997 ± 0.065	5.892AlaSer: 5.892 ± 0.066	6.976AlaThr: 6.976 ± 0.057	11.975AlaVal: 11.975 ± 0.073	1.867AlaTrp: 1.867 ± 0.032	2.872AlaTyr: 2.872 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.12CysAla: 1.12 ± 0.022	0.089CysCys: 0.089 ± 0.007	0.486CysAsp: 0.486 ± 0.014	0.417CysGlu: 0.417 ± 0.014	0.234CysPhe: 0.234 ± 0.011	1.017CysGly: 1.017 ± 0.021	0.196CysHis: 0.196 ± 0.009	0.171CysIle: 0.171 ± 0.009	0.14CysLys: 0.14 ± 0.008	0.768CysLeu: 0.768 ± 0.019	0.123CysMet: 0.123 ± 0.007	0.132CysAsn: 0.132 ± 0.007	0.477CysPro: 0.477 ± 0.017	0.159CysGln: 0.159 ± 0.008	0.619CysArg: 0.619 ± 0.017	0.434CysSer: 0.434 ± 0.014	0.526CysThr: 0.526 ± 0.013	0.702CysVal: 0.702 ± 0.017	0.126CysTrp: 0.126 ± 0.007	0.156CysTyr: 0.156 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.059AspAla: 8.059 ± 0.055	0.441AspCys: 0.441 ± 0.014	3.958AspAsp: 3.958 ± 0.041	4.086AspGlu: 4.086 ± 0.046	1.7AspPhe: 1.7 ± 0.03	6.616AspGly: 6.616 ± 0.064	1.463AspHis: 1.463 ± 0.027	2.064AspIle: 2.064 ± 0.03	1.509AspLys: 1.509 ± 0.034	6.226AspLeu: 6.226 ± 0.048	0.926AspMet: 0.926 ± 0.02	0.962AspAsn: 0.962 ± 0.02	4.566AspPro: 4.566 ± 0.053	1.467AspGln: 1.467 ± 0.024	4.976AspArg: 4.976 ± 0.051	2.654AspSer: 2.654 ± 0.034	3.318AspThr: 3.318 ± 0.039	5.043AspVal: 5.043 ± 0.044	1.049AspTrp: 1.049 ± 0.023	1.104AspTyr: 1.104 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.265GluAla: 7.265 ± 0.068	0.393GluCys: 0.393 ± 0.013	2.936GluAsp: 2.936 ± 0.043	3.575GluGlu: 3.575 ± 0.046	1.484GluPhe: 1.484 ± 0.027	4.621GluGly: 4.621 ± 0.047	1.583GluHis: 1.583 ± 0.026	2.201GluIle: 2.201 ± 0.034	1.713GluLys: 1.713 ± 0.035	6.91GluLeu: 6.91 ± 0.066	0.927GluMet: 0.927 ± 0.019	1.065GluAsn: 1.065 ± 0.023	3.322GluPro: 3.322 ± 0.039	2.27GluGln: 2.27 ± 0.035	5.701GluArg: 5.701 ± 0.053	2.663GluSer: 2.663 ± 0.031	3.008GluThr: 3.008 ± 0.038	4.356GluVal: 4.356 ± 0.051	0.799GluTrp: 0.799 ± 0.02	1.151GluTyr: 1.151 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.646PheAla: 3.646 ± 0.038	0.272PheCys: 0.272 ± 0.011	2.072PheAsp: 2.072 ± 0.032	1.431PheGlu: 1.431 ± 0.024	0.865PhePhe: 0.865 ± 0.02	3.05PheGly: 3.05 ± 0.037	0.621PheHis: 0.621 ± 0.018	0.76PheIle: 0.76 ± 0.018	0.579PheLys: 0.579 ± 0.017	2.591PheLeu: 2.591 ± 0.038	0.43PheMet: 0.43 ± 0.013	0.559PheAsn: 0.559 ± 0.014	1.367PhePro: 1.367 ± 0.023	0.663PheGln: 0.663 ± 0.016	1.837PheArg: 1.837 ± 0.03	1.461PheSer: 1.461 ± 0.028	1.995PheThr: 1.995 ± 0.031	2.268PheVal: 2.268 ± 0.034	0.434PheTrp: 0.434 ± 0.015	0.568PheTyr: 0.568 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.058GlyAla: 11.058 ± 0.079	0.852GlyCys: 0.852 ± 0.023	5.444GlyAsp: 5.444 ± 0.051	5.272GlyGlu: 5.272 ± 0.053	2.855GlyPhe: 2.855 ± 0.037	8.92GlyGly: 8.92 ± 0.09	2.38GlyHis: 2.38 ± 0.031	3.44GlyIle: 3.44 ± 0.037	2.83GlyLys: 2.83 ± 0.045	9.359GlyLeu: 9.359 ± 0.072	1.992GlyMet: 1.992 ± 0.03	1.753GlyAsn: 1.753 ± 0.033	4.976GlyPro: 4.976 ± 0.049	2.576GlyGln: 2.576 ± 0.04	7.642GlyArg: 7.642 ± 0.065	5.261GlySer: 5.261 ± 0.056	6.37GlyThr: 6.37 ± 0.062	7.587GlyVal: 7.587 ± 0.061	1.667GlyTrp: 1.667 ± 0.028	2.256GlyTyr: 2.256 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	2.777HisAla: 2.777 ± 0.035	0.234HisCys: 0.234 ± 0.009	1.445HisAsp: 1.445 ± 0.024	1.306HisGlu: 1.306 ± 0.028	0.656HisPhe: 0.656 ± 0.019	2.429HisGly: 2.429 ± 0.033	0.719HisHis: 0.719 ± 0.018	0.741HisIle: 0.741 ± 0.018	0.425HisLys: 0.425 ± 0.013	2.45HisLeu: 2.45 ± 0.037	0.364HisMet: 0.364 ± 0.013	0.383HisAsn: 0.383 ± 0.013	1.876HisPro: 1.876 ± 0.031	0.649HisGln: 0.649 ± 0.015	2.125HisArg: 2.125 ± 0.029	1.005HisSer: 1.005 ± 0.02	1.405HisThr: 1.405 ± 0.023	1.775HisVal: 1.775 ± 0.026	0.385HisTrp: 0.385 ± 0.013	0.515HisTyr: 0.515 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.695IleAla: 4.695 ± 0.05	0.292IleCys: 0.292 ± 0.01	2.298IleAsp: 2.298 ± 0.032	1.995IleGlu: 1.995 ± 0.037	0.718IlePhe: 0.718 ± 0.019	3.64IleGly: 3.64 ± 0.037	0.662IleHis: 0.662 ± 0.018	0.856IleIle: 0.856 ± 0.022	0.855IleLys: 0.855 ± 0.022	2.475IleLeu: 2.475 ± 0.038	0.435IleMet: 0.435 ± 0.014	0.719IleAsn: 0.719 ± 0.021	1.726IlePro: 1.726 ± 0.027	0.722IleGln: 0.722 ± 0.018	2.212IleArg: 2.212 ± 0.034	1.671IleSer: 1.671 ± 0.029	2.2IleThr: 2.2 ± 0.031	2.739IleVal: 2.739 ± 0.04	0.364IleTrp: 0.364 ± 0.011	0.523IleTyr: 0.523 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	3.282LysAla: 3.282 ± 0.051	0.141LysCys: 0.141 ± 0.008	1.629LysAsp: 1.629 ± 0.033	1.428LysGlu: 1.428 ± 0.027	0.547LysPhe: 0.547 ± 0.017	2.226LysGly: 2.226 ± 0.04	0.494LysHis: 0.494 ± 0.015	0.955LysIle: 0.955 ± 0.021	1.191LysLys: 1.191 ± 0.032	2.177LysLeu: 2.177 ± 0.032	0.445LysMet: 0.445 ± 0.014	0.632LysAsn: 0.632 ± 0.021	1.569LysPro: 1.569 ± 0.034	0.77LysGln: 0.77 ± 0.02	1.624LysArg: 1.624 ± 0.028	1.355LysSer: 1.355 ± 0.027	1.454LysThr: 1.454 ± 0.029	2.13LysVal: 2.13 ± 0.04	0.317LysTrp: 0.317 ± 0.013	0.524LysTyr: 0.524 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.525LeuAla: 14.525 ± 0.1	0.867LeuCys: 0.867 ± 0.022	6.734LeuAsp: 6.734 ± 0.055	4.583LeuGlu: 4.583 ± 0.044	2.628LeuPhe: 2.628 ± 0.031	9.15LeuGly: 9.15 ± 0.065	2.382LeuHis: 2.382 ± 0.032	3.294LeuIle: 3.294 ± 0.046	2.242LeuLys: 2.242 ± 0.043	10.973LeuLeu: 10.973 ± 0.094	1.725LeuMet: 1.725 ± 0.028	1.707LeuAsn: 1.707 ± 0.028	6.3LeuPro: 6.3 ± 0.061	2.161LeuGln: 2.161 ± 0.029	8.578LeuArg: 8.578 ± 0.067	5.355LeuSer: 5.355 ± 0.046	6.851LeuThr: 6.851 ± 0.06	8.673LeuVal: 8.673 ± 0.076	1.288LeuTrp: 1.288 ± 0.025	1.803LeuTyr: 1.803 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.277MetAla: 2.277 ± 0.031	0.151MetCys: 0.151 ± 0.007	0.936MetAsp: 0.936 ± 0.02	0.811MetGlu: 0.811 ± 0.024	0.473MetPhe: 0.473 ± 0.015	1.382MetGly: 1.382 ± 0.024	0.367MetHis: 0.367 ± 0.014	0.688MetIle: 0.688 ± 0.016	0.488MetLys: 0.488 ± 0.014	1.685MetLeu: 1.685 ± 0.027	0.313MetMet: 0.313 ± 0.013	0.48MetAsn: 0.48 ± 0.017	1.149MetPro: 1.149 ± 0.022	0.448MetGln: 0.448 ± 0.014	1.464MetArg: 1.464 ± 0.03	1.341MetSer: 1.341 ± 0.022	1.564MetThr: 1.564 ± 0.024	1.315MetVal: 1.315 ± 0.024	0.224MetTrp: 0.224 ± 0.009	0.339MetTyr: 0.339 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.155AsnAla: 2.155 ± 0.034	0.161AsnCys: 0.161 ± 0.009	1.005AsnAsp: 1.005 ± 0.023	0.844AsnGlu: 0.844 ± 0.02	0.48AsnPhe: 0.48 ± 0.014	1.935AsnGly: 1.935 ± 0.033	0.406AsnHis: 0.406 ± 0.012	0.658AsnIle: 0.658 ± 0.02	0.472AsnLys: 0.472 ± 0.016	1.679AsnLeu: 1.679 ± 0.025	0.299AsnMet: 0.299 ± 0.011	0.444AsnAsn: 0.444 ± 0.015	1.345AsnPro: 1.345 ± 0.023	0.503AsnGln: 0.503 ± 0.016	1.286AsnArg: 1.286 ± 0.023	0.925AsnSer: 0.925 ± 0.022	1.103AsnThr: 1.103 ± 0.021	1.391AsnVal: 1.391 ± 0.027	0.307AsnTrp: 0.307 ± 0.012	0.411AsnTyr: 0.411 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	7.905ProAla: 7.905 ± 0.066	0.361ProCys: 0.361 ± 0.013	4.669ProAsp: 4.669 ± 0.046	4.394ProGlu: 4.394 ± 0.047	1.539ProPhe: 1.539 ± 0.026	6.331ProGly: 6.331 ± 0.059	1.48ProHis: 1.48 ± 0.026	1.284ProIle: 1.284 ± 0.022	1.391ProLys: 1.391 ± 0.028	5.307ProLeu: 5.307 ± 0.057	1.039ProMet: 1.039 ± 0.022	0.875ProAsn: 0.875 ± 0.024	3.296ProPro: 3.296 ± 0.061	1.713ProGln: 1.713 ± 0.032	3.914ProArg: 3.914 ± 0.045	3.124ProSer: 3.124 ± 0.045	3.258ProThr: 3.258 ± 0.036	5.354ProVal: 5.354 ± 0.05	0.895ProTrp: 0.895 ± 0.019	1.376ProTyr: 1.376 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.455GlnAla: 3.455 ± 0.042	0.175GlnCys: 0.175 ± 0.009	1.457GlnAsp: 1.457 ± 0.026	1.492GlnGlu: 1.492 ± 0.029	0.678GlnPhe: 0.678 ± 0.019	2.31GlnGly: 2.31 ± 0.035	0.66GlnHis: 0.66 ± 0.017	1.056GlnIle: 1.056 ± 0.022	0.681GlnLys: 0.681 ± 0.018	2.975GlnLeu: 2.975 ± 0.036	0.496GlnMet: 0.496 ± 0.014	0.492GlnAsn: 0.492 ± 0.016	1.545GlnPro: 1.545 ± 0.03	1.217GlnGln: 1.217 ± 0.032	2.366GlnArg: 2.366 ± 0.032	1.258GlnSer: 1.258 ± 0.02	1.329GlnThr: 1.329 ± 0.023	2.223GlnVal: 2.223 ± 0.029	0.464GlnTrp: 0.464 ± 0.013	0.628GlnTyr: 0.628 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.724ArgAla: 9.724 ± 0.063	0.594ArgCys: 0.594 ± 0.016	4.444ArgAsp: 4.444 ± 0.041	4.795ArgGlu: 4.795 ± 0.055	2.317ArgPhe: 2.317 ± 0.036	5.978ArgGly: 5.978 ± 0.051	2.202ArgHis: 2.202 ± 0.031	3.205ArgIle: 3.205 ± 0.043	1.844ArgLys: 1.844 ± 0.029	8.552ArgLeu: 8.552 ± 0.068	1.713ArgMet: 1.713 ± 0.026	1.4ArgAsn: 1.4 ± 0.026	4.646ArgPro: 4.646 ± 0.052	2.238ArgGln: 2.238 ± 0.035	7.429ArgArg: 7.429 ± 0.07	3.983ArgSer: 3.983 ± 0.039	5.595ArgThr: 5.595 ± 0.047	5.953ArgVal: 5.953 ± 0.059	1.35ArgTrp: 1.35 ± 0.026	1.74ArgTyr: 1.74 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	6.509SerAla: 6.509 ± 0.053	0.424SerCys: 0.424 ± 0.013	2.767SerAsp: 2.767 ± 0.037	2.492SerGlu: 2.492 ± 0.037	1.537SerPhe: 1.537 ± 0.025	5.94SerGly: 5.94 ± 0.059	1.06SerHis: 1.06 ± 0.023	1.398SerIle: 1.398 ± 0.023	1.21SerLys: 1.21 ± 0.025	4.85SerLeu: 4.85 ± 0.043	1.075SerMet: 1.075 ± 0.022	0.895SerAsn: 0.895 ± 0.019	3.142SerPro: 3.142 ± 0.04	1.256SerGln: 1.256 ± 0.023	3.662SerArg: 3.662 ± 0.037	2.813SerSer: 2.813 ± 0.037	3.034SerThr: 3.034 ± 0.039	4.361SerVal: 4.361 ± 0.042	0.913SerTrp: 0.913 ± 0.022	1.233SerTyr: 1.233 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	8.716ThrAla: 8.716 ± 0.068	0.436ThrCys: 0.436 ± 0.013	3.705ThrAsp: 3.705 ± 0.045	3.409ThrGlu: 3.409 ± 0.039	1.662ThrPhe: 1.662 ± 0.027	6.621ThrGly: 6.621 ± 0.056	1.308ThrHis: 1.308 ± 0.026	1.703ThrIle: 1.703 ± 0.026	1.381ThrLys: 1.381 ± 0.03	5.737ThrLeu: 5.737 ± 0.054	0.971ThrMet: 0.971 ± 0.022	0.989ThrAsn: 0.989 ± 0.022	4.197ThrPro: 4.197 ± 0.043	1.302ThrGln: 1.302 ± 0.021	3.975ThrArg: 3.975 ± 0.044	3.199ThrSer: 3.199 ± 0.039	3.869ThrThr: 3.869 ± 0.05	6.066ThrVal: 6.066 ± 0.052	0.897ThrTrp: 0.897 ± 0.019	1.566ThrTyr: 1.566 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.696ValAla: 10.696 ± 0.078	0.76ValCys: 0.76 ± 0.019	5.175ValAsp: 5.175 ± 0.048	4.77ValGlu: 4.77 ± 0.05	2.404ValPhe: 2.404 ± 0.033	6.657ValGly: 6.657 ± 0.062	1.968ValHis: 1.968 ± 0.033	2.904ValIle: 2.904 ± 0.045	1.871ValLys: 1.871 ± 0.029	9.284ValLeu: 9.284 ± 0.072	1.461ValMet: 1.461 ± 0.027	1.636ValAsn: 1.636 ± 0.029	5.225ValPro: 5.225 ± 0.047	2.079ValGln: 2.079 ± 0.029	7.174ValArg: 7.174 ± 0.062	4.322ValSer: 4.322 ± 0.044	5.647ValThr: 5.647 ± 0.051	7.944ValVal: 7.944 ± 0.073	1.126ValTrp: 1.126 ± 0.019	1.549ValTyr: 1.549 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.62TrpAla: 1.62 ± 0.028	0.155TrpCys: 0.155 ± 0.008	0.889TrpAsp: 0.889 ± 0.022	0.74TrpGlu: 0.74 ± 0.02	0.514TrpPhe: 0.514 ± 0.015	1.079TrpGly: 1.079 ± 0.023	0.371TrpHis: 0.371 ± 0.012	0.543TrpIle: 0.543 ± 0.017	0.409TrpLys: 0.409 ± 0.013	1.772TrpLeu: 1.772 ± 0.028	0.301TrpMet: 0.301 ± 0.012	0.408TrpAsn: 0.408 ± 0.014	0.798TrpPro: 0.798 ± 0.017	0.606TrpGln: 0.606 ± 0.018	1.309TrpArg: 1.309 ± 0.026	0.921TrpSer: 0.921 ± 0.018	1.03TrpThr: 1.03 ± 0.022	1.02TrpVal: 1.02 ± 0.02	0.335TrpTrp: 0.335 ± 0.013	0.346TrpTyr: 0.346 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.831TyrAla: 2.831 ± 0.034	0.175TyrCys: 0.175 ± 0.008	1.59TyrAsp: 1.59 ± 0.039	1.43TyrGlu: 1.43 ± 0.026	0.679TyrPhe: 0.679 ± 0.017	2.304TyrGly: 2.304 ± 0.035	0.385TyrHis: 0.385 ± 0.012	0.477TyrIle: 0.477 ± 0.012	0.477TyrLys: 0.477 ± 0.017	1.987TyrLeu: 1.987 ± 0.03	0.269TyrMet: 0.269 ± 0.012	0.399TyrAsn: 0.399 ± 0.013	1.048TyrPro: 1.048 ± 0.02	0.549TyrGln: 0.549 ± 0.017	1.811TyrArg: 1.811 ± 0.027	0.937TyrSer: 0.937 ± 0.019	1.176TyrThr: 1.176 ± 0.025	1.776TyrVal: 1.776 ± 0.025	0.359TyrTrp: 0.359 ± 0.012	0.451TyrTyr: 0.451 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8464 proteins (2391914 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---