Muribaculaceae bacterium Z82

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C9JDP7|A0A7C9JDP7_9BACT Uncharacterized protein OS=Muribaculaceae bacterium Z82 OX=2304548 GN=D1639_06380 PE=4 SV=1
MM1 pKa = 7.51ALSLLVLGACSAPAPTDD18 pKa = 3.45NSEE21 pKa = 4.0KK22 pKa = 10.58AAPEE26 pKa = 4.08TPAAPLPASDD36 pKa = 4.59VMKK39 pKa = 10.88NDD41 pKa = 5.13DD42 pKa = 3.68PTQTSGFFAFDD53 pKa = 3.05SALFQGKK60 pKa = 7.86LTGCSYY66 pKa = 11.28AQAGTLLVCADD77 pKa = 4.02EE78 pKa = 5.28LYY80 pKa = 10.85LYY82 pKa = 8.18DD83 pKa = 3.94TASGTVLAHH92 pKa = 6.59CPAPAPMRR100 pKa = 11.84DD101 pKa = 3.61FGAAPFKK108 pKa = 10.87DD109 pKa = 4.42GIVLTVMGDD118 pKa = 3.52AGAVAYY124 pKa = 9.7IYY126 pKa = 10.7DD127 pKa = 3.95RR128 pKa = 11.84EE129 pKa = 4.29LKK131 pKa = 10.87LEE133 pKa = 3.99EE134 pKa = 3.98TLALEE139 pKa = 4.6EE140 pKa = 4.83LLSGDD145 pKa = 3.8PVVDD149 pKa = 4.6PGCVAASSDD158 pKa = 3.24GRR160 pKa = 11.84QLAIAGLSALYY171 pKa = 10.34LYY173 pKa = 9.99DD174 pKa = 4.31RR175 pKa = 11.84PAGQLTTLLDD185 pKa = 3.59TAQGVGGASCMATSLNGAAFTPDD208 pKa = 3.61DD209 pKa = 4.1DD210 pKa = 3.91RR211 pKa = 11.84VAFFGDD217 pKa = 3.77GFSAEE222 pKa = 4.46SGNSEE227 pKa = 4.35DD228 pKa = 4.99SAPVWGTIGIDD239 pKa = 3.64GSEE242 pKa = 5.08LEE244 pKa = 4.5LDD246 pKa = 3.36WLEE249 pKa = 4.33SEE251 pKa = 4.95GVEE254 pKa = 4.48EE255 pKa = 4.39MLRR258 pKa = 11.84ADD260 pKa = 3.44GRR262 pKa = 11.84LFFPPAISHH271 pKa = 6.26VDD273 pKa = 3.33GGLPWVDD280 pKa = 3.31AVSGAAHH287 pKa = 7.11RR288 pKa = 11.84LAFSASDD295 pKa = 3.34EE296 pKa = 4.6GGDD299 pKa = 3.65GIYY302 pKa = 10.64VSEE305 pKa = 4.08QGKK308 pKa = 9.27YY309 pKa = 10.02AATAEE314 pKa = 4.38LGDD317 pKa = 3.83VLTVRR322 pKa = 11.84VYY324 pKa = 11.01AVDD327 pKa = 3.42SGEE330 pKa = 4.2LLATEE335 pKa = 4.74TIEE338 pKa = 4.75VDD340 pKa = 2.88DD341 pKa = 3.87SLYY344 pKa = 10.78VGRR347 pKa = 11.84IPQVILLDD355 pKa = 4.09DD356 pKa = 4.11AQAAIVLLGCGIEE369 pKa = 4.51EE370 pKa = 4.62IDD372 pKa = 3.58TAVATFSFGVV382 pKa = 3.31

Molecular weight: 39.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C9NBS4|A0A7C9NBS4_9BACT 4Fe-4S dicluster domain-containing protein OS=Muribaculaceae bacterium Z82 OX=2304548 GN=D1639_09755 PE=4 SV=1
MM1 pKa = 7.75PNGRR5 pKa = 11.84KK6 pKa = 8.9QPASASFRR14 pKa = 11.84RR15 pKa = 11.84TASSPGHH22 pKa = 5.56RR23 pKa = 11.84RR24 pKa = 11.84SRR26 pKa = 11.84SARR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84QGRR34 pKa = 11.84SRR36 pKa = 11.84TPAAA40 pKa = 4.06

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2214

0

2214

760548

27

23345

343.5

37.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.285 ± 0.104

1.564 ± 0.048

6.14 ± 0.11

6.359 ± 0.056

3.709 ± 0.043

8.657 ± 0.05

1.617 ± 0.021

4.351 ± 0.055

3.483 ± 0.038

9.187 ± 0.11

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.042

2.835 ± 0.093

4.511 ± 0.033

3.167 ± 0.031

5.975 ± 0.088

6.107 ± 0.048

5.181 ± 0.129

8.159 ± 0.047

1.187 ± 0.029

2.924 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski