Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales; Picrophilaceae; Picrophilus; Picrophilus torridus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1535 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6L0X3|Q6L0X3_PICTO Uncharacterized protein OS=Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) OX=263820 GN=PTO0794 PE=4 SV=1
MM1 pKa = 7.87LYY3 pKa = 10.85NIMTMASIEE12 pKa = 3.95IDD14 pKa = 3.55EE15 pKa = 4.5NVYY18 pKa = 10.73KK19 pKa = 10.69YY20 pKa = 10.4ISSSGDD26 pKa = 3.42DD27 pKa = 3.05VSLFVNKK34 pKa = 9.07MLRR37 pKa = 11.84SYY39 pKa = 10.8VEE41 pKa = 3.64AMQRR45 pKa = 11.84PDD47 pKa = 3.22FNEE50 pKa = 3.93YY51 pKa = 9.44TEE53 pKa = 4.95HH54 pKa = 7.03GNGDD58 pKa = 3.35

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6L0G7|Q6L0G7_PICTO Glycinamide ribonucleotide synthetase OS=Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) OX=263820 GN=PTO0950 PE=4 SV=1
MM1 pKa = 7.75TIFQGRR7 pKa = 11.84ATRR10 pKa = 11.84KK11 pKa = 9.03PSGGKK16 pKa = 9.23LRR18 pKa = 11.84PNHH21 pKa = 5.61SKK23 pKa = 10.3RR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 8.0EE27 pKa = 4.11LGRR30 pKa = 11.84EE31 pKa = 4.02PTLTRR36 pKa = 11.84LGDD39 pKa = 3.41RR40 pKa = 11.84EE41 pKa = 4.0LRR43 pKa = 11.84KK44 pKa = 9.65IRR46 pKa = 11.84SYY48 pKa = 11.45GGNSKK53 pKa = 10.27FALLRR58 pKa = 11.84CDD60 pKa = 3.41YY61 pKa = 11.58ANVYY65 pKa = 9.2NPKK68 pKa = 10.59DD69 pKa = 3.14KK70 pKa = 8.68TTRR73 pKa = 11.84KK74 pKa = 9.98VKK76 pKa = 10.49INTVKK81 pKa = 10.56EE82 pKa = 4.03NSADD86 pKa = 3.28PHH88 pKa = 5.49YY89 pKa = 10.44VQRR92 pKa = 11.84NIMNRR97 pKa = 11.84GTVISTEE104 pKa = 4.06LGDD107 pKa = 4.04ARR109 pKa = 11.84ITSRR113 pKa = 11.84PGQDD117 pKa = 2.93GVINAVLLL125 pKa = 4.09

Molecular weight: 14.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1535

0

1535

471263

24

1667

307.0

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.613 ± 0.06

0.618 ± 0.017

5.866 ± 0.059

5.662 ± 0.061

4.857 ± 0.056

6.521 ± 0.059

1.488 ± 0.022

11.078 ± 0.075

6.9 ± 0.073

8.623 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.061 ± 0.026

6.617 ± 0.067

3.488 ± 0.039

1.464 ± 0.023

4.422 ± 0.05

7.44 ± 0.059

4.374 ± 0.048

5.732 ± 0.046

0.734 ± 0.017

5.444 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski