Niastella koreensis (strain DSM 17620 / KACC 11465 / NBRC 106392 / GR20-10)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Niastella; Niastella koreensis

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8T718|G8T718_NIAKG RagB/SusD domain-containing protein OS=Niastella koreensis (strain DSM 17620 / KACC 11465 / NBRC 106392 / GR20-10) OX=700598 GN=Niako_2700 PE=3 SV=1
MM1 pKa = 7.29YY2 pKa = 9.94SYY4 pKa = 11.68SLLEE8 pKa = 4.16PGCFYY13 pKa = 10.95LIQEE17 pKa = 4.72KK18 pKa = 10.32EE19 pKa = 4.24DD20 pKa = 3.77EE21 pKa = 4.55PVTLIKK27 pKa = 9.85ITVEE31 pKa = 3.89TDD33 pKa = 2.52QCLYY37 pKa = 8.05ITQYY41 pKa = 11.14EE42 pKa = 4.37DD43 pKa = 3.26MPVMVWKK50 pKa = 10.51KK51 pKa = 10.62KK52 pKa = 9.96NDD54 pKa = 4.37PIFDD58 pKa = 3.82ILEE61 pKa = 4.3LLSDD65 pKa = 4.21DD66 pKa = 5.45KK67 pKa = 11.53VKK69 pKa = 10.38EE70 pKa = 3.95WEE72 pKa = 3.97AVYY75 pKa = 11.03NDD77 pKa = 3.6STDD80 pKa = 2.96AYY82 pKa = 10.92YY83 pKa = 10.63EE84 pKa = 4.18EE85 pKa = 5.3EE86 pKa = 4.62DD87 pKa = 5.05DD88 pKa = 4.32EE89 pKa = 4.61

Molecular weight: 10.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8TC75|G8TC75_NIAKG Uncharacterized protein OS=Niastella koreensis (strain DSM 17620 / KACC 11465 / NBRC 106392 / GR20-10) OX=700598 GN=Niako_4103 PE=4 SV=1
MM1 pKa = 7.55SIVITLVGLIGVIMGRR17 pKa = 11.84QALVRR22 pKa = 11.84TNRR25 pKa = 11.84RR26 pKa = 11.84IGSARR31 pKa = 11.84PKK33 pKa = 10.59AIAALLLGLTGIVASGLHH51 pKa = 5.94LAISSGGFGTGSGRR65 pKa = 11.84AGAIVAMVLGFIGAAFGWLALARR88 pKa = 11.84SKK90 pKa = 9.12QTAGHH95 pKa = 6.21GSLRR99 pKa = 3.75

Molecular weight: 9.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7171

0

7171

2631446

26

5321

367.0

41.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.7 ± 0.026

0.894 ± 0.01

5.167 ± 0.017

5.177 ± 0.027

4.692 ± 0.021

7.002 ± 0.031

1.919 ± 0.015

6.721 ± 0.023

6.502 ± 0.027

9.268 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.014

5.763 ± 0.033

4.08 ± 0.017

4.044 ± 0.016

4.106 ± 0.018

6.161 ± 0.021

6.321 ± 0.033

6.545 ± 0.023

1.372 ± 0.011

4.261 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski