Moritella viscosa
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4219 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A090IN07|A0A090IN07_9GAMM Uncharacterized protein OS=Moritella viscosa OX=80854 GN=MVIS_4002 PE=4 SV=1
MM1 pKa = 7.64 ALYY4 pKa = 10.09 KK5 pKa = 10.41 KK6 pKa = 10.43 EE7 pKa = 4.18 FGAQFEE13 pKa = 5.03 LGFDD17 pKa = 3.61 LAKK20 pKa = 10.66 YY21 pKa = 10.25 DD22 pKa = 3.55 WLTDD26 pKa = 3.86 KK27 pKa = 10.77 SWHH30 pKa = 6.41 NDD32 pKa = 2.74 VCPSFTYY39 pKa = 8.8 KK40 pKa = 10.48 TSDD43 pKa = 3.02 GYY45 pKa = 11.18 FILWVDD51 pKa = 4.04 YY52 pKa = 10.57 IDD54 pKa = 4.02 PEE56 pKa = 4.01 QRR58 pKa = 11.84 EE59 pKa = 4.38 FEE61 pKa = 4.11 QCRR64 pKa = 11.84 YY65 pKa = 7.62 TVISAINEE73 pKa = 3.79 GDD75 pKa = 3.44 EE76 pKa = 4.49 DD77 pKa = 4.31 SPEE80 pKa = 3.87 VFSFSEE86 pKa = 4.19 SVTIFEE92 pKa = 5.24 SEE94 pKa = 4.05 SDD96 pKa = 3.5 DD97 pKa = 4.65 DD98 pKa = 5.43 LIEE101 pKa = 4.32 FLSCVVAA108 pKa = 4.9
Molecular weight: 12.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.935
Patrickios 0.947
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A090I8Y8|A0A090I8Y8_9GAMM Coproporphyrinogen-III oxidase OS=Moritella viscosa OX=80854 GN=hemN PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.2 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.89 ATVGGRR28 pKa = 11.84 KK29 pKa = 9.02 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 KK40 pKa = 6.41 TLSAA44 pKa = 4.15
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4219
0
4219
1380057
36
5571
327.1
36.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.326 ± 0.044
1.049 ± 0.012
5.633 ± 0.031
5.774 ± 0.034
4.193 ± 0.027
6.417 ± 0.039
2.139 ± 0.02
7.129 ± 0.031
5.685 ± 0.032
10.439 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.017
4.874 ± 0.031
3.61 ± 0.021
4.414 ± 0.034
4.118 ± 0.033
6.902 ± 0.031
5.709 ± 0.028
6.722 ± 0.03
1.145 ± 0.012
3.166 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here