Epulopiscium sp. SCG-B10WGA-EpuloB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2MGA4|A0A1V2MGA4_9FIRM Uncharacterized protein OS=Epulopiscium sp. SCG-B10WGA-EpuloB OX=1712380 GN=AN643_00010 PE=4 SV=1
MM1 pKa = 7.18YY2 pKa = 10.46LSDD5 pKa = 4.22NSIFDD10 pKa = 3.5IYY12 pKa = 11.35NEE14 pKa = 3.97FVGVVDD20 pKa = 5.38EE21 pKa = 6.56DD22 pKa = 3.1IDD24 pKa = 4.26YY25 pKa = 11.7YY26 pKa = 11.35NFNYY30 pKa = 10.62DD31 pKa = 3.74KK32 pKa = 11.44DD33 pKa = 4.49NIDD36 pKa = 4.72SIINNYY42 pKa = 10.59DD43 pKa = 3.51SIFGDD48 pKa = 3.94NILDD52 pKa = 3.61ASTILVADD60 pKa = 3.62EE61 pKa = 4.65TFNSFIDD68 pKa = 4.17FSKK71 pKa = 10.8LSSFEE76 pKa = 3.75FGNIVSQEE84 pKa = 3.83FFEE87 pKa = 4.5EE88 pKa = 4.43HH89 pKa = 6.0LSSYY93 pKa = 10.46YY94 pKa = 10.38VNQVLMYY101 pKa = 8.52MDD103 pKa = 3.86VYY105 pKa = 11.14DD106 pKa = 4.58RR107 pKa = 11.84YY108 pKa = 11.18IMYY111 pKa = 10.6SKK113 pKa = 11.15GDD115 pKa = 3.76DD116 pKa = 3.67YY117 pKa = 11.56FIAYY121 pKa = 9.25YY122 pKa = 10.75NSLEE126 pKa = 4.23SSDD129 pKa = 3.72VRR131 pKa = 11.84YY132 pKa = 7.14TAPGGRR138 pKa = 11.84NTIDD142 pKa = 3.31FNRR145 pKa = 11.84YY146 pKa = 4.78VHH148 pKa = 6.34GFIVKK153 pKa = 8.33FSPDD157 pKa = 3.2YY158 pKa = 10.46GISVEE163 pKa = 4.49SYY165 pKa = 9.79YY166 pKa = 11.28DD167 pKa = 4.34DD168 pKa = 4.36IYY170 pKa = 11.38DD171 pKa = 3.81YY172 pKa = 11.86DD173 pKa = 6.02DD174 pKa = 5.24IIATNDD180 pKa = 3.09YY181 pKa = 10.69RR182 pKa = 11.84VFFVSSAKK190 pKa = 8.9FTYY193 pKa = 10.68DD194 pKa = 4.38DD195 pKa = 4.58ADD197 pKa = 3.8LGLARR202 pKa = 11.84EE203 pKa = 4.33VLYY206 pKa = 11.13GMGDD210 pKa = 3.76LHH212 pKa = 9.11SMIVPPDD219 pKa = 3.34LSFDD223 pKa = 4.01FNQSIEE229 pKa = 4.1NLVVDD234 pKa = 6.05LEE236 pKa = 4.33LTDD239 pKa = 5.89DD240 pKa = 5.54DD241 pKa = 5.36FLEE244 pKa = 4.75LDD246 pKa = 3.8SVLDD250 pKa = 3.46VDD252 pKa = 6.28DD253 pKa = 5.58IISVLRR259 pKa = 11.84DD260 pKa = 3.1KK261 pKa = 11.4DD262 pKa = 3.69LDD264 pKa = 3.79SDD266 pKa = 4.09AGAGMDD272 pKa = 4.05DD273 pKa = 3.87VPVGFFDD280 pKa = 6.73DD281 pKa = 3.46ILSFFDD287 pKa = 4.67NILSSFDD294 pKa = 3.34KK295 pKa = 11.25VLGFFDD301 pKa = 6.57DD302 pKa = 3.87ILSSFDD308 pKa = 3.3KK309 pKa = 11.27VLGFFDD315 pKa = 4.78DD316 pKa = 3.99VFVNLSEE323 pKa = 4.12NLKK326 pKa = 10.84SLFIPDD332 pKa = 3.78FEE334 pKa = 5.63DD335 pKa = 3.07IKK337 pKa = 11.28SRR339 pKa = 11.84FYY341 pKa = 9.11EE342 pKa = 4.26TKK344 pKa = 10.51DD345 pKa = 3.22KK346 pKa = 11.57VSVKK350 pKa = 10.56LGFHH354 pKa = 5.29NWILFEE360 pKa = 5.27DD361 pKa = 4.32ALTLYY366 pKa = 10.51AKK368 pKa = 10.2KK369 pKa = 10.05PKK371 pKa = 10.35FEE373 pKa = 3.91IFIYY377 pKa = 9.58GQAVTLIDD385 pKa = 3.72LSYY388 pKa = 10.76FEE390 pKa = 6.21GYY392 pKa = 8.64MFYY395 pKa = 10.74LQDD398 pKa = 3.71IIKK401 pKa = 10.52GFFSFLLLLYY411 pKa = 9.28NYY413 pKa = 10.78KK414 pKa = 9.61MVLYY418 pKa = 9.99LLRR421 pKa = 11.84SSHH424 pKa = 6.96FIKK427 pKa = 10.66GGKK430 pKa = 8.53

Molecular weight: 49.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2MFA3|A0A1V2MFA3_9FIRM PRC domain-containing protein OS=Epulopiscium sp. SCG-B10WGA-EpuloB OX=1712380 GN=AN643_00935 PE=4 SV=1
MM1 pKa = 7.3TEE3 pKa = 3.95KK4 pKa = 10.52EE5 pKa = 3.83YY6 pKa = 11.26DD7 pKa = 3.37LSIFKK12 pKa = 10.42IKK14 pKa = 10.54EE15 pKa = 3.99FVHH18 pKa = 6.7LPLALEE24 pKa = 4.52LSHH27 pKa = 6.79GLLLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84CHH36 pKa = 5.78EE37 pKa = 3.86VTEE40 pKa = 4.69GVGGRR45 pKa = 11.84WQRR48 pKa = 11.84YY49 pKa = 4.54STLPLRR55 pKa = 11.84GVPHH59 pKa = 5.65QRR61 pKa = 11.84GGLYY65 pKa = 10.29GWSKK69 pKa = 8.88RR70 pKa = 11.84AKK72 pKa = 9.98RR73 pKa = 11.84RR74 pKa = 11.84TT75 pKa = 3.39

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

732

0

732

208488

30

1434

284.8

32.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.126 ± 0.1

1.053 ± 0.041

5.852 ± 0.092

7.334 ± 0.114

4.208 ± 0.074

6.098 ± 0.122

1.612 ± 0.036

9.503 ± 0.117

7.924 ± 0.139

9.068 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.045

6.013 ± 0.084

3.233 ± 0.078

3.188 ± 0.065

3.389 ± 0.07

6.284 ± 0.084

5.649 ± 0.091

5.981 ± 0.073

0.73 ± 0.03

4.114 ± 0.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski