Amino acid dipepetide frequency for Epulopiscium sp. SCG-B10WGA-EpuloB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.252AlaAla: 3.252 ± 0.171
0.624AlaCys: 0.624 ± 0.06
3.276AlaAsp: 3.276 ± 0.121
4.355AlaGlu: 4.355 ± 0.185
2.432AlaPhe: 2.432 ± 0.126
3.952AlaGly: 3.952 ± 0.202
1.041AlaHis: 1.041 ± 0.084
6.115AlaIle: 6.115 ± 0.205
5.391AlaLys: 5.391 ± 0.191
5.717AlaLeu: 5.717 ± 0.203
1.736AlaMet: 1.736 ± 0.094
3.405AlaAsn: 3.405 ± 0.151
1.746AlaPro: 1.746 ± 0.087
2.345AlaGln: 2.345 ± 0.131
2.154AlaArg: 2.154 ± 0.126
3.679AlaSer: 3.679 ± 0.149
3.765AlaThr: 3.765 ± 0.146
3.65AlaVal: 3.65 ± 0.197
0.36AlaTrp: 0.36 ± 0.049
2.264AlaTyr: 2.264 ± 0.092
0.0AlaXaa: 0.0 ± 0.0
Cys
0.604CysAla: 0.604 ± 0.057
0.125CysCys: 0.125 ± 0.024
0.643CysAsp: 0.643 ± 0.054
0.643CysGlu: 0.643 ± 0.053
0.619CysPhe: 0.619 ± 0.051
0.878CysGly: 0.878 ± 0.078
0.221CysHis: 0.221 ± 0.034
0.883CysIle: 0.883 ± 0.07
0.763CysLys: 0.763 ± 0.072
0.911CysLeu: 0.911 ± 0.073
0.288CysMet: 0.288 ± 0.034
0.518CysAsn: 0.518 ± 0.064
0.365CysPro: 0.365 ± 0.047
0.297CysGln: 0.297 ± 0.037
0.379CysArg: 0.379 ± 0.043
0.705CysSer: 0.705 ± 0.059
0.552CysThr: 0.552 ± 0.056
0.609CysVal: 0.609 ± 0.058
0.053CysTrp: 0.053 ± 0.015
0.475CysTyr: 0.475 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
3.477AspAla: 3.477 ± 0.276
0.561AspCys: 0.561 ± 0.051
3.017AspAsp: 3.017 ± 0.16
4.058AspGlu: 4.058 ± 0.15
3.022AspPhe: 3.022 ± 0.16
3.295AspGly: 3.295 ± 0.149
0.854AspHis: 0.854 ± 0.06
6.231AspIle: 6.231 ± 0.203
4.533AspLys: 4.533 ± 0.17
5.727AspLeu: 5.727 ± 0.223
1.535AspMet: 1.535 ± 0.095
3.463AspAsn: 3.463 ± 0.14
2.274AspPro: 2.274 ± 0.13
1.693AspGln: 1.693 ± 0.094
1.923AspArg: 1.923 ± 0.119
3.075AspSer: 3.075 ± 0.132
3.051AspThr: 3.051 ± 0.131
3.314AspVal: 3.314 ± 0.147
0.633AspTrp: 0.633 ± 0.062
2.787AspTyr: 2.787 ± 0.124
0.0AspXaa: 0.0 ± 0.0
Glu
4.192GluAla: 4.192 ± 0.165
0.638GluCys: 0.638 ± 0.057
4.202GluAsp: 4.202 ± 0.175
6.341GluGlu: 6.341 ± 0.321
2.959GluPhe: 2.959 ± 0.133
3.741GluGly: 3.741 ± 0.155
1.18GluHis: 1.18 ± 0.065
7.996GluIle: 7.996 ± 0.273
6.346GluLys: 6.346 ± 0.238
6.993GluLeu: 6.993 ± 0.234
2.077GluMet: 2.077 ± 0.117
4.734GluAsn: 4.734 ± 0.176
1.756GluPro: 1.756 ± 0.105
2.528GluGln: 2.528 ± 0.154
2.437GluArg: 2.437 ± 0.119
3.569GluSer: 3.569 ± 0.138
3.358GluThr: 3.358 ± 0.13
4.461GluVal: 4.461 ± 0.183
0.585GluTrp: 0.585 ± 0.058
3.247GluTyr: 3.247 ± 0.121
0.0GluXaa: 0.0 ± 0.0
Phe
2.557PheAla: 2.557 ± 0.13
0.518PheCys: 0.518 ± 0.05
2.772PheAsp: 2.772 ± 0.138
3.175PheGlu: 3.175 ± 0.155
1.856PhePhe: 1.856 ± 0.125
3.003PheGly: 3.003 ± 0.145
0.609PheHis: 0.609 ± 0.056
3.741PheIle: 3.741 ± 0.148
3.142PheLys: 3.142 ± 0.156
3.463PheLeu: 3.463 ± 0.157
1.07PheMet: 1.07 ± 0.071
2.686PheAsn: 2.686 ± 0.112
1.281PhePro: 1.281 ± 0.084
1.031PheGln: 1.031 ± 0.066
1.391PheArg: 1.391 ± 0.087
3.055PheSer: 3.055 ± 0.155
2.206PheThr: 2.206 ± 0.109
2.432PheVal: 2.432 ± 0.108
0.321PheTrp: 0.321 ± 0.039
1.77PheTyr: 1.77 ± 0.1
0.0PheXaa: 0.0 ± 0.0
Gly
3.794GlyAla: 3.794 ± 0.163
0.868GlyCys: 0.868 ± 0.081
3.42GlyAsp: 3.42 ± 0.239
4.072GlyGlu: 4.072 ± 0.226
2.676GlyPhe: 2.676 ± 0.127
4.063GlyGly: 4.063 ± 0.193
1.18GlyHis: 1.18 ± 0.088
5.679GlyIle: 5.679 ± 0.19
4.485GlyLys: 4.485 ± 0.191
5.593GlyLeu: 5.593 ± 0.183
1.621GlyMet: 1.621 ± 0.099
3.055GlyAsn: 3.055 ± 0.145
1.597GlyPro: 1.597 ± 0.364
2.259GlyGln: 2.259 ± 0.122
2.216GlyArg: 2.216 ± 0.101
3.664GlySer: 3.664 ± 0.154
3.468GlyThr: 3.468 ± 0.141
4.077GlyVal: 4.077 ± 0.153
0.465GlyTrp: 0.465 ± 0.05
2.729GlyTyr: 2.729 ± 0.121
0.0GlyXaa: 0.0 ± 0.0
His
0.859HisAla: 0.859 ± 0.065
0.187HisCys: 0.187 ± 0.03
0.873HisAsp: 0.873 ± 0.068
0.998HisGlu: 0.998 ± 0.083
0.695HisPhe: 0.695 ± 0.054
0.988HisGly: 0.988 ± 0.072
0.446HisHis: 0.446 ± 0.049
1.405HisIle: 1.405 ± 0.092
1.151HisLys: 1.151 ± 0.07
1.554HisLeu: 1.554 ± 0.098
0.417HisMet: 0.417 ± 0.048
1.07HisAsn: 1.07 ± 0.076
0.801HisPro: 0.801 ± 0.061
0.484HisGln: 0.484 ± 0.056
0.58HisArg: 0.58 ± 0.048
1.065HisSer: 1.065 ± 0.067
0.734HisThr: 0.734 ± 0.063
0.974HisVal: 0.974 ± 0.067
0.158HisTrp: 0.158 ± 0.032
0.676HisTyr: 0.676 ± 0.061
0.0HisXaa: 0.0 ± 0.0
Ile
5.856IleAla: 5.856 ± 0.175
1.103IleCys: 1.103 ± 0.082
5.856IleAsp: 5.856 ± 0.185
7.554IleGlu: 7.554 ± 0.242
3.928IlePhe: 3.928 ± 0.159
5.866IleGly: 5.866 ± 0.233
1.329IleHis: 1.329 ± 0.078
8.739IleIle: 8.739 ± 0.294
7.617IleLys: 7.617 ± 0.235
8.672IleLeu: 8.672 ± 0.275
2.365IleMet: 2.365 ± 0.107
5.487IleAsn: 5.487 ± 0.164
3.338IlePro: 3.338 ± 0.156
2.792IleGln: 2.792 ± 0.133
3.257IleArg: 3.257 ± 0.132
6.47IleSer: 6.47 ± 0.192
4.998IleThr: 4.998 ± 0.158
5.708IleVal: 5.708 ± 0.188
0.576IleTrp: 0.576 ± 0.054
3.521IleTyr: 3.521 ± 0.127
0.0IleXaa: 0.0 ± 0.0
Lys
4.561LysAla: 4.561 ± 0.196
0.849LysCys: 0.849 ± 0.09
4.475LysAsp: 4.475 ± 0.16
6.763LysGlu: 6.763 ± 0.224
3.036LysPhe: 3.036 ± 0.138
3.947LysGly: 3.947 ± 0.179
1.055LysHis: 1.055 ± 0.079
7.526LysIle: 7.526 ± 0.231
6.715LysLys: 6.715 ± 0.294
7.118LysLeu: 7.118 ± 0.232
2.245LysMet: 2.245 ± 0.097
5.526LysAsn: 5.526 ± 0.185
1.851LysPro: 1.851 ± 0.09
2.772LysGln: 2.772 ± 0.125
2.739LysArg: 2.739 ± 0.136
4.489LysSer: 4.489 ± 0.143
4.259LysThr: 4.259 ± 0.316
4.936LysVal: 4.936 ± 0.203
0.6LysTrp: 0.6 ± 0.056
3.78LysTyr: 3.78 ± 0.288
0.0LysXaa: 0.0 ± 0.0
Leu
6.259LeuAla: 6.259 ± 0.225
0.969LeuCys: 0.969 ± 0.074
5.866LeuAsp: 5.866 ± 0.184
7.238LeuGlu: 7.238 ± 0.215
3.573LeuPhe: 3.573 ± 0.167
5.43LeuGly: 5.43 ± 0.176
1.271LeuHis: 1.271 ± 0.087
7.804LeuIle: 7.804 ± 0.223
7.209LeuLys: 7.209 ± 0.23
7.765LeuLeu: 7.765 ± 0.276
2.254LeuMet: 2.254 ± 0.125
5.247LeuAsn: 5.247 ± 0.154
3.3LeuPro: 3.3 ± 0.133
2.475LeuGln: 2.475 ± 0.111
3.252LeuArg: 3.252 ± 0.143
6.461LeuSer: 6.461 ± 0.191
5.314LeuThr: 5.314 ± 0.179
5.07LeuVal: 5.07 ± 0.18
0.537LeuTrp: 0.537 ± 0.058
3.382LeuTyr: 3.382 ± 0.134
0.0LeuXaa: 0.0 ± 0.0
Met
2.029MetAla: 2.029 ± 0.104
0.245MetCys: 0.245 ± 0.03
1.794MetAsp: 1.794 ± 0.098
1.703MetGlu: 1.703 ± 0.069
1.055MetPhe: 1.055 ± 0.075
1.506MetGly: 1.506 ± 0.106
0.412MetHis: 0.412 ± 0.044
2.341MetIle: 2.341 ± 0.138
2.326MetLys: 2.326 ± 0.103
2.475MetLeu: 2.475 ± 0.11
0.662MetMet: 0.662 ± 0.066
1.458MetAsn: 1.458 ± 0.089
1.022MetPro: 1.022 ± 0.077
0.988MetGln: 0.988 ± 0.068
0.811MetArg: 0.811 ± 0.063
1.664MetSer: 1.664 ± 0.088
1.333MetThr: 1.333 ± 0.088
1.496MetVal: 1.496 ± 0.094
0.168MetTrp: 0.168 ± 0.027
0.907MetTyr: 0.907 ± 0.071
0.0MetXaa: 0.0 ± 0.0
Asn
3.137AsnAla: 3.137 ± 0.134
0.58AsnCys: 0.58 ± 0.053
2.916AsnAsp: 2.916 ± 0.122
3.645AsnGlu: 3.645 ± 0.132
2.7AsnPhe: 2.7 ± 0.122
3.242AsnGly: 3.242 ± 0.16
1.06AsnHis: 1.06 ± 0.065
6.394AsnIle: 6.394 ± 0.202
4.864AsnLys: 4.864 ± 0.172
6.024AsnLeu: 6.024 ± 0.179
1.621AsnMet: 1.621 ± 0.088
4.226AsnAsn: 4.226 ± 0.17
2.408AsnPro: 2.408 ± 0.122
1.952AsnGln: 1.952 ± 0.128
1.693AsnArg: 1.693 ± 0.099
4.067AsnSer: 4.067 ± 0.167
3.218AsnThr: 3.218 ± 0.132
3.343AsnVal: 3.343 ± 0.15
0.494AsnTrp: 0.494 ± 0.056
2.542AsnTyr: 2.542 ± 0.106
0.0AsnXaa: 0.0 ± 0.0
Pro
1.986ProAla: 1.986 ± 0.133
0.317ProCys: 0.317 ± 0.04
2.302ProAsp: 2.302 ± 0.15
2.576ProGlu: 2.576 ± 0.135
1.151ProPhe: 1.151 ± 0.073
1.919ProGly: 1.919 ± 0.116
0.59ProHis: 0.59 ± 0.057
2.959ProIle: 2.959 ± 0.145
2.307ProLys: 2.307 ± 0.107
2.557ProLeu: 2.557 ± 0.126
0.758ProMet: 0.758 ± 0.074
2.053ProAsn: 2.053 ± 0.116
0.897ProPro: 0.897 ± 0.096
1.017ProGln: 1.017 ± 0.172
0.959ProArg: 0.959 ± 0.073
2.053ProSer: 2.053 ± 0.107
2.187ProThr: 2.187 ± 0.119
2.11ProVal: 2.11 ± 0.11
0.259ProTrp: 0.259 ± 0.041
1.372ProTyr: 1.372 ± 0.074
0.005ProXaa: 0.005 ± 0.004
Gln
2.12GlnAla: 2.12 ± 0.103
0.269GlnCys: 0.269 ± 0.039
1.871GlnAsp: 1.871 ± 0.109
2.226GlnGlu: 2.226 ± 0.139
1.161GlnPhe: 1.161 ± 0.076
1.842GlnGly: 1.842 ± 0.324
0.508GlnHis: 0.508 ± 0.051
2.921GlnIle: 2.921 ± 0.127
2.628GlnLys: 2.628 ± 0.129
2.82GlnLeu: 2.82 ± 0.131
1.002GlnMet: 1.002 ± 0.073
2.168GlnAsn: 2.168 ± 0.137
0.931GlnPro: 0.931 ± 0.077
1.046GlnGln: 1.046 ± 0.082
1.127GlnArg: 1.127 ± 0.079
1.851GlnSer: 1.851 ± 0.092
2.048GlnThr: 2.048 ± 0.12
1.803GlnVal: 1.803 ± 0.094
0.206GlnTrp: 0.206 ± 0.033
1.329GlnTyr: 1.329 ± 0.091
0.0GlnXaa: 0.0 ± 0.0
Arg
1.962ArgAla: 1.962 ± 0.101
0.326ArgCys: 0.326 ± 0.043
1.631ArgAsp: 1.631 ± 0.092
2.662ArgGlu: 2.662 ± 0.136
1.415ArgPhe: 1.415 ± 0.083
2.048ArgGly: 2.048 ± 0.137
0.609ArgHis: 0.609 ± 0.058
3.108ArgIle: 3.108 ± 0.132
2.844ArgLys: 2.844 ± 0.128
3.089ArgLeu: 3.089 ± 0.161
1.036ArgMet: 1.036 ± 0.08
1.775ArgAsn: 1.775 ± 0.097
1.132ArgPro: 1.132 ± 0.085
1.002ArgGln: 1.002 ± 0.077
1.468ArgArg: 1.468 ± 0.098
1.991ArgSer: 1.991 ± 0.134
1.808ArgThr: 1.808 ± 0.088
2.298ArgVal: 2.298 ± 0.115
0.221ArgTrp: 0.221 ± 0.035
1.463ArgTyr: 1.463 ± 0.077
0.0ArgXaa: 0.0 ± 0.0
Ser
3.645SerAla: 3.645 ± 0.167
0.542SerCys: 0.542 ± 0.059
3.626SerAsp: 3.626 ± 0.164
4.053SerGlu: 4.053 ± 0.139
2.859SerPhe: 2.859 ± 0.145
4.384SerGly: 4.384 ± 0.19
1.098SerHis: 1.098 ± 0.074
6.197SerIle: 6.197 ± 0.223
4.729SerLys: 4.729 ± 0.153
5.506SerLeu: 5.506 ± 0.207
1.645SerMet: 1.645 ± 0.094
3.861SerAsn: 3.861 ± 0.173
1.736SerPro: 1.736 ± 0.108
2.211SerGln: 2.211 ± 0.142
2.034SerArg: 2.034 ± 0.107
4.432SerSer: 4.432 ± 0.194
3.928SerThr: 3.928 ± 0.174
3.535SerVal: 3.535 ± 0.137
0.408SerTrp: 0.408 ± 0.044
2.413SerTyr: 2.413 ± 0.105
0.0SerXaa: 0.0 ± 0.0
Thr
3.65ThrAla: 3.65 ± 0.179
0.456ThrCys: 0.456 ± 0.045
3.166ThrAsp: 3.166 ± 0.153
3.573ThrGlu: 3.573 ± 0.146
2.288ThrPhe: 2.288 ± 0.105
4.178ThrGly: 4.178 ± 0.316
0.859ThrHis: 0.859 ± 0.067
5.348ThrIle: 5.348 ± 0.18
4.144ThrLys: 4.144 ± 0.149
5.29ThrLeu: 5.29 ± 0.18
1.381ThrMet: 1.381 ± 0.085
3.334ThrAsn: 3.334 ± 0.132
2.245ThrPro: 2.245 ± 0.109
1.847ThrGln: 1.847 ± 0.094
1.708ThrArg: 1.708 ± 0.112
3.602ThrSer: 3.602 ± 0.132
3.602ThrThr: 3.602 ± 0.205
3.319ThrVal: 3.319 ± 0.128
0.369ThrTrp: 0.369 ± 0.052
2.134ThrTyr: 2.134 ± 0.117
0.0ThrXaa: 0.0 ± 0.0
Val
4.585ValAla: 4.585 ± 0.178
0.734ValCys: 0.734 ± 0.066
3.741ValAsp: 3.741 ± 0.157
4.571ValGlu: 4.571 ± 0.16
2.326ValPhe: 2.326 ± 0.099
3.703ValGly: 3.703 ± 0.148
0.964ValHis: 0.964 ± 0.079
5.252ValIle: 5.252 ± 0.171
4.518ValLys: 4.518 ± 0.211
5.209ValLeu: 5.209 ± 0.178
1.602ValMet: 1.602 ± 0.094
2.945ValAsn: 2.945 ± 0.111
1.957ValPro: 1.957 ± 0.092
1.65ValGln: 1.65 ± 0.093
2.082ValArg: 2.082 ± 0.115
3.818ValSer: 3.818 ± 0.147
3.617ValThr: 3.617 ± 0.146
3.986ValVal: 3.986 ± 0.172
0.417ValTrp: 0.417 ± 0.045
2.134ValTyr: 2.134 ± 0.103
0.0ValXaa: 0.0 ± 0.0
Trp
0.504TrpAla: 0.504 ± 0.049
0.139TrpCys: 0.139 ± 0.025
0.384TrpAsp: 0.384 ± 0.044
0.355TrpGlu: 0.355 ± 0.045
0.341TrpPhe: 0.341 ± 0.044
0.48TrpGly: 0.48 ± 0.053
0.125TrpHis: 0.125 ± 0.023
0.657TrpIle: 0.657 ± 0.055
0.393TrpLys: 0.393 ± 0.038
0.556TrpLeu: 0.556 ± 0.045
0.211TrpMet: 0.211 ± 0.035
0.494TrpAsn: 0.494 ± 0.05
0.221TrpPro: 0.221 ± 0.033
0.297TrpGln: 0.297 ± 0.04
0.273TrpArg: 0.273 ± 0.038
0.528TrpSer: 0.528 ± 0.058
0.393TrpThr: 0.393 ± 0.048
0.408TrpVal: 0.408 ± 0.045
0.163TrpTrp: 0.163 ± 0.026
0.384TrpTyr: 0.384 ± 0.058
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.23TyrAla: 2.23 ± 0.106
0.48TyrCys: 0.48 ± 0.051
2.691TyrAsp: 2.691 ± 0.141
2.777TyrGlu: 2.777 ± 0.126
1.986TyrPhe: 1.986 ± 0.101
2.518TyrGly: 2.518 ± 0.126
0.705TyrHis: 0.705 ± 0.055
3.732TyrIle: 3.732 ± 0.152
3.137TyrLys: 3.137 ± 0.124
3.636TyrLeu: 3.636 ± 0.134
0.863TyrMet: 0.863 ± 0.072
2.628TyrAsn: 2.628 ± 0.131
1.477TyrPro: 1.477 ± 0.102
1.19TyrGln: 1.19 ± 0.081
1.405TyrArg: 1.405 ± 0.08
2.604TyrSer: 2.604 ± 0.116
2.652TyrThr: 2.652 ± 0.304
2.283TyrVal: 2.283 ± 0.115
0.312TyrTrp: 0.312 ± 0.048
1.832TyrTyr: 1.832 ± 0.114
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.005XaaIle: 0.005 ± 0.004
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.005XaaXaa: 0.005 ± 0.004
Statistics based on 732 proteins (208488 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski