Erysiphe pulchra

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Erysiphe

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S4PTU3|A0A2S4PTU3_9PEZI SURF6-domain-containing protein (Fragment) OS=Erysiphe pulchra OX=225359 GN=EPUL_003951 PE=3 SV=1
MM1 pKa = 7.42TGATSEE7 pKa = 3.93AGYY10 pKa = 10.93DD11 pKa = 3.45VVVDD15 pKa = 3.65VDD17 pKa = 3.45EE18 pKa = 6.42DD19 pKa = 4.15GDD21 pKa = 4.32LGHH24 pKa = 7.38TDD26 pKa = 3.4LQEE29 pKa = 4.41DD30 pKa = 4.7LEE32 pKa = 4.49FHH34 pKa = 7.04SSNFNNDD41 pKa = 2.2ASTASNSRR49 pKa = 11.84KK50 pKa = 10.23GPGAGTGLPIPATANTGSPKK70 pKa = 10.52YY71 pKa = 9.24MIWSLPFYY79 pKa = 10.89AQFFDD84 pKa = 3.45VDD86 pKa = 3.79TAAVLSRR93 pKa = 11.84CWATIYY99 pKa = 10.37PRR101 pKa = 11.84ANFIEE106 pKa = 4.25VLGGNPDD113 pKa = 3.24LYY115 pKa = 11.23GPLWIATTVVFILFLGGTINQYY137 pKa = 10.95LSEE140 pKa = 4.4TNGTQFVYY148 pKa = 10.83DD149 pKa = 4.04FTLLSGG155 pKa = 3.76

Molecular weight: 16.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S4PIJ3|A0A2S4PIJ3_9PEZI Uncharacterized protein (Fragment) OS=Erysiphe pulchra OX=225359 GN=EPUL_006201 PE=4 SV=1
MM1 pKa = 7.73ALTFPVPSTQQILQTCLSAAKK22 pKa = 9.73RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84SSSSEE30 pKa = 3.61KK31 pKa = 10.73APTSRR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.89ARR41 pKa = 11.84SSRR44 pKa = 11.84TSTHH48 pKa = 6.62ASPSRR53 pKa = 11.84QLSRR57 pKa = 11.84ALSVPTAVIAARR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84KK72 pKa = 8.33WQTNDD77 pKa = 2.96YY78 pKa = 9.65QRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84DD83 pKa = 3.26SHH85 pKa = 5.49EE86 pKa = 4.16APRR89 pKa = 11.84HH90 pKa = 4.44CAPRR94 pKa = 11.84RR95 pKa = 11.84TSTRR99 pKa = 11.84RR100 pKa = 11.84HH101 pKa = 5.65CEE103 pKa = 3.14ITGRR107 pKa = 11.84RR108 pKa = 11.84GGDD111 pKa = 3.0GTTSVGGSGGRR122 pKa = 11.84DD123 pKa = 3.3PEE125 pKa = 4.57GGQGVRR131 pKa = 11.84RR132 pKa = 11.84GGRR135 pKa = 11.84PEE137 pKa = 3.87KK138 pKa = 10.44SEE140 pKa = 4.37HH141 pKa = 6.16NGGEE145 pKa = 3.87QGQGDD150 pKa = 3.95RR151 pKa = 11.84GQHH154 pKa = 5.17EE155 pKa = 4.43RR156 pKa = 11.84EE157 pKa = 4.03QPEE160 pKa = 4.02GNAAEE165 pKa = 4.47TRR167 pKa = 11.84RR168 pKa = 11.84HH169 pKa = 4.19GHH171 pKa = 5.19VLEE174 pKa = 4.5TDD176 pKa = 3.85PPQLRR181 pKa = 11.84LFHH184 pKa = 7.17EE185 pKa = 5.08DD186 pKa = 2.96GGGCRR191 pKa = 11.84AHH193 pKa = 6.8SGPEE197 pKa = 3.73RR198 pKa = 11.84PEE200 pKa = 3.75QQAYY204 pKa = 7.17WCQEE208 pKa = 3.73GYY210 pKa = 9.91RR211 pKa = 11.84WCAPGLALRR220 pKa = 11.84QRR222 pKa = 11.84CGFQEE227 pKa = 4.98DD228 pKa = 3.46ILVRR232 pKa = 11.84RR233 pKa = 11.84FRR235 pKa = 11.84AAAQVFQEE243 pKa = 4.83PEE245 pKa = 3.8DD246 pKa = 3.93CLLEE250 pKa = 4.89RR251 pKa = 11.84GAGTEE256 pKa = 4.2GG257 pKa = 3.21

Molecular weight: 28.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6853

0

6853

3364779

18

4885

491.0

55.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.446 ± 0.025

1.332 ± 0.011

5.45 ± 0.018

6.545 ± 0.031

3.884 ± 0.017

5.505 ± 0.024

2.257 ± 0.012

6.542 ± 0.02

6.455 ± 0.028

9.17 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.935 ± 0.01

5.165 ± 0.02

5.098 ± 0.024

4.101 ± 0.021

5.734 ± 0.022

9.192 ± 0.036

5.777 ± 0.016

5.34 ± 0.017

1.22 ± 0.011

2.853 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski