Streptococcus phage CHPC1042
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8F9R9|A0A3G8F9R9_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1042 OX=2365016 GN=CHPC1042_0038 PE=4 SV=1
MM1 pKa = 7.13 VNWVDD6 pKa = 4.58 ANGNDD11 pKa = 3.63 IPDD14 pKa = 4.12 GADD17 pKa = 3.05 QEE19 pKa = 5.1 FKK21 pKa = 11.28 AGMFFSFAGDD31 pKa = 3.56 EE32 pKa = 4.15 NNITDD37 pKa = 4.03 TGQGGYY43 pKa = 10.16 YY44 pKa = 9.73 GGYY47 pKa = 8.6 YY48 pKa = 9.56 FRR50 pKa = 11.84 KK51 pKa = 9.96 FEE53 pKa = 4.13 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.859
IPC_protein 3.77
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.91
Patrickios 0.401
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A3G8F9V1|A0A3G8F9V1_9CAUD ORF6C domain-containing protein OS=Streptococcus phage CHPC1042 OX=2365016 GN=CHPC1042_0035 PE=4 SV=1
MM1 pKa = 7.83 ADD3 pKa = 3.75 FGVSLLEE10 pKa = 4.01 ARR12 pKa = 11.84 RR13 pKa = 11.84 MTLKK17 pKa = 10.54 EE18 pKa = 3.35 MRR20 pKa = 11.84 LYY22 pKa = 10.44 QKK24 pKa = 10.59 AYY26 pKa = 9.79 KK27 pKa = 10.05 KK28 pKa = 10.52 RR29 pKa = 11.84 FLNKK33 pKa = 9.43 EE34 pKa = 3.69 RR35 pKa = 11.84 EE36 pKa = 4.18 IYY38 pKa = 10.27 QLAFLNRR45 pKa = 11.84 LANATTRR52 pKa = 11.84 DD53 pKa = 3.11 GKK55 pKa = 10.61 RR56 pKa = 11.84 YY57 pKa = 9.56 HH58 pKa = 6.45 FEE60 pKa = 3.92 KK61 pKa = 11.17 FEE63 pKa = 4.24 DD64 pKa = 4.34 FYY66 pKa = 11.61 NAKK69 pKa = 9.59 EE70 pKa = 3.87 RR71 pKa = 11.84 ARR73 pKa = 11.84 EE74 pKa = 3.84 VLGEE78 pKa = 4.21 KK79 pKa = 8.61 ITNKK83 pKa = 10.26 KK84 pKa = 9.81 LLEE87 pKa = 4.02 RR88 pKa = 11.84 ARR90 pKa = 11.84 NNLNYY95 pKa = 10.06 KK96 pKa = 9.85 KK97 pKa = 10.46 EE98 pKa = 3.94 RR99 pKa = 11.84 GLLDD103 pKa = 3.22 GRR105 pKa = 4.46
Molecular weight: 12.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 9.531
IPC_protein 9.721
Toseland 10.335
ProMoST 10.043
Dawson 10.482
Bjellqvist 10.116
Wikipedia 10.628
Rodwell 10.935
Grimsley 10.54
Solomon 10.511
Lehninger 10.496
Nozaki 10.292
DTASelect 10.116
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.394
Patrickios 10.643
IPC_peptide 10.526
IPC2_peptide 8.639
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
12710
47
1545
208.4
23.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.388 ± 1.032
0.669 ± 0.135
6.121 ± 0.436
7.081 ± 0.694
4.162 ± 0.147
6.467 ± 0.527
1.503 ± 0.205
6.656 ± 0.331
8.623 ± 0.632
7.71 ± 0.335
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.463 ± 0.304
5.618 ± 0.367
2.73 ± 0.207
4.115 ± 0.26
4.327 ± 0.376
6.648 ± 0.552
6.105 ± 0.318
6.452 ± 0.292
1.204 ± 0.187
3.958 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here