Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae; Pyrobaculum; Pyrobaculum aerophilum

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2590 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8ZT04|PGP_PYRAE Phosphoglycolate phosphatase OS=Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827) OX=178306 GN=PAE3495 PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 5.19EE3 pKa = 4.44SYY5 pKa = 10.13IWPIVFVDD13 pKa = 3.13VDD15 pKa = 3.62RR16 pKa = 11.84NYY18 pKa = 11.72

Molecular weight: 2.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8ZTY7|Q8ZTY7_PYRAE Oligosaccharyl transferase OS=Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827) OX=178306 GN=PAE3030 PE=3 SV=1
MM1 pKa = 7.58ARR3 pKa = 11.84NKK5 pKa = 10.29PLGKK9 pKa = 10.05KK10 pKa = 9.73LRR12 pKa = 11.84LAAAFKK18 pKa = 9.46XNRR21 pKa = 11.84NPPVWVVVKK30 pKa = 8.44TKK32 pKa = 10.61RR33 pKa = 11.84RR34 pKa = 11.84VTRR37 pKa = 11.84SPARR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.8WRR45 pKa = 11.84RR46 pKa = 11.84VKK48 pKa = 10.84LKK50 pKa = 10.56AA51 pKa = 3.65

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2590

0

2590

654950

18

2785

252.9

28.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.886 ± 0.073

0.871 ± 0.02

4.295 ± 0.036

6.995 ± 0.075

3.633 ± 0.025

7.676 ± 0.047

1.511 ± 0.019

6.293 ± 0.045

5.677 ± 0.045

10.568 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.926 ± 0.02

2.604 ± 0.033

4.988 ± 0.043

2.083 ± 0.025

6.546 ± 0.046

4.935 ± 0.038

4.407 ± 0.042

9.321 ± 0.054

1.474 ± 0.025

4.308 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski