Amino acid dipepetide frequency for Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.372AlaAla: 10.372 ± 0.259
0.617AlaCys: 0.617 ± 0.033
3.585AlaAsp: 3.585 ± 0.069
7.202AlaGlu: 7.202 ± 0.15
3.681AlaPhe: 3.681 ± 0.095
6.929AlaGly: 6.929 ± 0.137
1.367AlaHis: 1.367 ± 0.045
5.052AlaIle: 5.052 ± 0.101
5.316AlaLys: 5.316 ± 0.098
12.709AlaLeu: 12.709 ± 0.209
1.96AlaMet: 1.96 ± 0.054
1.818AlaAsn: 1.818 ± 0.059
3.916AlaPro: 3.916 ± 0.078
2.096AlaGln: 2.096 ± 0.065
5.978AlaArg: 5.978 ± 0.121
5.185AlaSer: 5.185 ± 0.106
4.422AlaThr: 4.422 ± 0.091
11.363AlaVal: 11.363 ± 0.151
1.283AlaTrp: 1.283 ± 0.055
4.013AlaTyr: 4.013 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
0.617CysAla: 0.617 ± 0.03
0.116CysCys: 0.116 ± 0.013
0.354CysAsp: 0.354 ± 0.024
0.487CysGlu: 0.487 ± 0.027
0.298CysPhe: 0.298 ± 0.024
1.058CysGly: 1.058 ± 0.041
0.159CysHis: 0.159 ± 0.016
0.496CysIle: 0.496 ± 0.03
0.588CysLys: 0.588 ± 0.031
0.646CysLeu: 0.646 ± 0.035
0.209CysMet: 0.209 ± 0.018
0.286CysAsn: 0.286 ± 0.018
0.812CysPro: 0.812 ± 0.04
0.218CysGln: 0.218 ± 0.017
0.531CysArg: 0.531 ± 0.032
0.411CysSer: 0.411 ± 0.025
0.418CysThr: 0.418 ± 0.027
0.664CysVal: 0.664 ± 0.04
0.096CysTrp: 0.096 ± 0.012
0.249CysTyr: 0.249 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.78AspAla: 3.78 ± 0.086
0.345AspCys: 0.345 ± 0.022
1.635AspAsp: 1.635 ± 0.055
2.971AspGlu: 2.971 ± 0.07
1.869AspPhe: 1.869 ± 0.043
2.935AspGly: 2.935 ± 0.084
0.487AspHis: 0.487 ± 0.026
3.069AspIle: 3.069 ± 0.066
2.4AspLys: 2.4 ± 0.069
4.672AspLeu: 4.672 ± 0.09
0.867AspMet: 0.867 ± 0.04
0.892AspAsn: 0.892 ± 0.042
2.78AspPro: 2.78 ± 0.065
0.687AspGln: 0.687 ± 0.036
2.241AspArg: 2.241 ± 0.056
1.686AspSer: 1.686 ± 0.055
1.438AspThr: 1.438 ± 0.052
5.542AspVal: 5.542 ± 0.094
0.776AspTrp: 0.776 ± 0.035
1.876AspTyr: 1.876 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
7.527GluAla: 7.527 ± 0.153
0.444GluCys: 0.444 ± 0.027
2.6GluAsp: 2.6 ± 0.064
6.73GluGlu: 6.73 ± 0.14
2.513GluPhe: 2.513 ± 0.057
4.889GluGly: 4.889 ± 0.09
0.785GluHis: 0.785 ± 0.036
4.945GluIle: 4.945 ± 0.1
4.613GluLys: 4.613 ± 0.097
7.845GluLeu: 7.845 ± 0.128
1.287GluMet: 1.287 ± 0.047
1.695GluAsn: 1.695 ± 0.06
2.498GluPro: 2.498 ± 0.067
1.165GluGln: 1.165 ± 0.047
5.095GluArg: 5.095 ± 0.105
2.29GluSer: 2.29 ± 0.058
2.4GluThr: 2.4 ± 0.07
7.179GluVal: 7.179 ± 0.113
0.927GluTrp: 0.927 ± 0.038
2.527GluTyr: 2.527 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.179PheAla: 3.179 ± 0.076
0.261PheCys: 0.261 ± 0.021
1.73PheAsp: 1.73 ± 0.047
2.07PheGlu: 2.07 ± 0.062
1.428PhePhe: 1.428 ± 0.048
2.742PheGly: 2.742 ± 0.059
0.609PheHis: 0.609 ± 0.031
2.583PheIle: 2.583 ± 0.068
2.499PheLys: 2.499 ± 0.062
3.791PheLeu: 3.791 ± 0.092
0.669PheMet: 0.669 ± 0.038
1.185PheAsn: 1.185 ± 0.047
1.447PhePro: 1.447 ± 0.048
0.834PheGln: 0.834 ± 0.034
1.88PheArg: 1.88 ± 0.052
2.212PheSer: 2.212 ± 0.062
2.054PheThr: 2.054 ± 0.062
2.877PheVal: 2.877 ± 0.074
0.565PheTrp: 0.565 ± 0.032
1.719PheTyr: 1.719 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
6.559GlyAla: 6.559 ± 0.128
0.727GlyCys: 0.727 ± 0.034
3.958GlyAsp: 3.958 ± 0.077
5.402GlyGlu: 5.402 ± 0.101
2.904GlyPhe: 2.904 ± 0.071
6.758GlyGly: 6.758 ± 0.115
1.215GlyHis: 1.215 ± 0.045
4.603GlyIle: 4.603 ± 0.091
4.167GlyLys: 4.167 ± 0.077
7.601GlyLeu: 7.601 ± 0.144
1.464GlyMet: 1.464 ± 0.05
1.883GlyAsn: 1.883 ± 0.062
3.107GlyPro: 3.107 ± 0.08
1.548GlyGln: 1.548 ± 0.051
5.587GlyArg: 5.587 ± 0.107
3.374GlySer: 3.374 ± 0.075
2.938GlyThr: 2.938 ± 0.066
8.65GlyVal: 8.65 ± 0.126
1.208GlyTrp: 1.208 ± 0.041
3.107GlyTyr: 3.107 ± 0.079
0.0GlyXaa: 0.0 ± 0.0
His
1.171HisAla: 1.171 ± 0.039
0.185HisCys: 0.185 ± 0.017
0.588HisAsp: 0.588 ± 0.029
0.806HisGlu: 0.806 ± 0.035
0.672HisPhe: 0.672 ± 0.031
1.429HisGly: 1.429 ± 0.05
0.318HisHis: 0.318 ± 0.021
1.079HisIle: 1.079 ± 0.04
0.737HisLys: 0.737 ± 0.034
1.463HisLeu: 1.463 ± 0.053
0.385HisMet: 0.385 ± 0.026
0.426HisAsn: 0.426 ± 0.023
0.919HisPro: 0.919 ± 0.043
0.232HisGln: 0.232 ± 0.02
0.907HisArg: 0.907 ± 0.031
0.733HisSer: 0.733 ± 0.033
0.624HisThr: 0.624 ± 0.03
1.525HisVal: 1.525 ± 0.048
0.227HisTrp: 0.227 ± 0.018
0.686HisTyr: 0.686 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.791IleAla: 6.791 ± 0.109
0.562IleCys: 0.562 ± 0.03
3.443IleAsp: 3.443 ± 0.087
4.031IleGlu: 4.031 ± 0.084
2.089IlePhe: 2.089 ± 0.06
4.576IleGly: 4.576 ± 0.087
1.006IleHis: 1.006 ± 0.042
4.074IleIle: 4.074 ± 0.104
3.751IleLys: 3.751 ± 0.079
5.318IleLeu: 5.318 ± 0.111
1.176IleMet: 1.176 ± 0.042
2.173IleAsn: 2.173 ± 0.073
3.005IlePro: 3.005 ± 0.07
1.167IleGln: 1.167 ± 0.04
3.643IleArg: 3.643 ± 0.084
4.005IleSer: 4.005 ± 0.083
3.394IleThr: 3.394 ± 0.071
4.987IleVal: 4.987 ± 0.096
0.707IleTrp: 0.707 ± 0.037
3.038IleTyr: 3.038 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
6.091LysAla: 6.091 ± 0.103
0.71LysCys: 0.71 ± 0.036
1.935LysAsp: 1.935 ± 0.062
3.787LysGlu: 3.787 ± 0.09
1.989LysPhe: 1.989 ± 0.053
3.263LysGly: 3.263 ± 0.079
0.759LysHis: 0.759 ± 0.036
4.417LysIle: 4.417 ± 0.089
3.374LysLys: 3.374 ± 0.081
5.665LysLeu: 5.665 ± 0.1
1.365LysMet: 1.365 ± 0.05
1.58LysAsn: 1.58 ± 0.051
2.87LysPro: 2.87 ± 0.076
0.983LysGln: 0.983 ± 0.04
4.408LysArg: 4.408 ± 0.091
2.703LysSer: 2.703 ± 0.059
2.922LysThr: 2.922 ± 0.061
4.019LysVal: 4.019 ± 0.099
1.073LysTrp: 1.073 ± 0.037
2.852LysTyr: 2.852 ± 0.069
0.002LysXaa: 0.002 ± 0.002
Leu
11.7LeuAla: 11.7 ± 0.186
0.734LeuCys: 0.734 ± 0.032
4.507LeuAsp: 4.507 ± 0.08
7.248LeuGlu: 7.248 ± 0.117
3.582LeuPhe: 3.582 ± 0.085
8.54LeuGly: 8.54 ± 0.137
1.591LeuHis: 1.591 ± 0.055
6.188LeuIle: 6.188 ± 0.106
6.758LeuLys: 6.758 ± 0.096
10.697LeuLeu: 10.697 ± 0.175
2.133LeuMet: 2.133 ± 0.051
2.941LeuAsn: 2.941 ± 0.077
4.976LeuPro: 4.976 ± 0.082
2.264LeuGln: 2.264 ± 0.065
7.126LeuArg: 7.126 ± 0.112
6.188LeuSer: 6.188 ± 0.1
4.735LeuThr: 4.735 ± 0.091
7.759LeuVal: 7.759 ± 0.12
1.348LeuTrp: 1.348 ± 0.054
4.664LeuTyr: 4.664 ± 0.095
0.0LeuXaa: 0.0 ± 0.0
Met
2.386MetAla: 2.386 ± 0.055
0.206MetCys: 0.206 ± 0.017
0.747MetAsp: 0.747 ± 0.033
1.367MetGlu: 1.367 ± 0.04
0.62MetPhe: 0.62 ± 0.03
1.626MetGly: 1.626 ± 0.056
0.31MetHis: 0.31 ± 0.023
0.939MetIle: 0.939 ± 0.041
1.09MetLys: 1.09 ± 0.041
1.863MetLeu: 1.863 ± 0.058
0.255MetMet: 0.255 ± 0.02
0.432MetAsn: 0.432 ± 0.027
1.27MetPro: 1.27 ± 0.049
0.399MetGln: 0.399 ± 0.025
1.461MetArg: 1.461 ± 0.046
1.237MetSer: 1.237 ± 0.041
0.811MetThr: 0.811 ± 0.036
1.052MetVal: 1.052 ± 0.036
0.366MetTrp: 0.366 ± 0.025
0.823MetTyr: 0.823 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
2.875AsnAla: 2.875 ± 0.07
0.347AsnCys: 0.347 ± 0.024
0.91AsnAsp: 0.91 ± 0.036
1.203AsnGlu: 1.203 ± 0.042
1.038AsnPhe: 1.038 ± 0.041
2.0AsnGly: 2.0 ± 0.056
0.264AsnHis: 0.264 ± 0.022
1.976AsnIle: 1.976 ± 0.074
1.542AsnLys: 1.542 ± 0.05
2.492AsnLeu: 2.492 ± 0.068
0.525AsnMet: 0.525 ± 0.027
0.768AsnAsn: 0.768 ± 0.036
1.838AsnPro: 1.838 ± 0.061
0.411AsnGln: 0.411 ± 0.026
1.312AsnArg: 1.312 ± 0.043
1.246AsnSer: 1.246 ± 0.047
1.221AsnThr: 1.221 ± 0.043
2.351AsnVal: 2.351 ± 0.07
0.457AsnTrp: 0.457 ± 0.025
1.269AsnTyr: 1.269 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
3.881ProAla: 3.881 ± 0.086
0.495ProCys: 0.495 ± 0.026
2.318ProAsp: 2.318 ± 0.062
3.53ProGlu: 3.53 ± 0.084
1.647ProPhe: 1.647 ± 0.053
3.762ProGly: 3.762 ± 0.079
1.095ProHis: 1.095 ± 0.044
2.634ProIle: 2.634 ± 0.066
2.173ProLys: 2.173 ± 0.067
5.344ProLeu: 5.344 ± 0.09
0.912ProMet: 0.912 ± 0.036
1.36ProAsn: 1.36 ± 0.044
3.88ProPro: 3.88 ± 0.116
1.432ProGln: 1.432 ± 0.046
3.141ProArg: 3.141 ± 0.081
2.759ProSer: 2.759 ± 0.079
2.472ProThr: 2.472 ± 0.077
4.165ProVal: 4.165 ± 0.077
0.768ProTrp: 0.768 ± 0.038
2.118ProTyr: 2.118 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
1.744GlnAla: 1.744 ± 0.049
0.246GlnCys: 0.246 ± 0.019
0.631GlnAsp: 0.631 ± 0.035
1.165GlnGlu: 1.165 ± 0.042
0.802GlnPhe: 0.802 ± 0.031
1.4GlnGly: 1.4 ± 0.039
0.289GlnHis: 0.289 ± 0.022
1.527GlnIle: 1.527 ± 0.043
1.038GlnLys: 1.038 ± 0.041
2.567GlnLeu: 2.567 ± 0.063
0.525GlnMet: 0.525 ± 0.03
0.592GlnAsn: 0.592 ± 0.033
1.02GlnPro: 1.02 ± 0.036
0.675GlnGln: 0.675 ± 0.042
1.647GlnArg: 1.647 ± 0.052
0.959GlnSer: 0.959 ± 0.04
0.977GlnThr: 0.977 ± 0.047
1.57GlnVal: 1.57 ± 0.055
0.437GlnTrp: 0.437 ± 0.023
1.017GlnTyr: 1.017 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
5.613ArgAla: 5.613 ± 0.1
0.65ArgCys: 0.65 ± 0.034
3.104ArgAsp: 3.104 ± 0.074
5.906ArgGlu: 5.906 ± 0.12
2.206ArgPhe: 2.206 ± 0.061
6.19ArgGly: 6.19 ± 0.107
1.208ArgHis: 1.208 ± 0.044
3.809ArgIle: 3.809 ± 0.09
3.058ArgLys: 3.058 ± 0.084
7.356ArgLeu: 7.356 ± 0.122
1.122ArgMet: 1.122 ± 0.038
1.563ArgAsn: 1.563 ± 0.05
3.098ArgPro: 3.098 ± 0.062
1.606ArgGln: 1.606 ± 0.056
5.709ArgArg: 5.709 ± 0.105
2.296ArgSer: 2.296 ± 0.06
2.023ArgThr: 2.023 ± 0.058
5.46ArgVal: 5.46 ± 0.093
0.995ArgTrp: 0.995 ± 0.053
2.49ArgTyr: 2.49 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
4.692SerAla: 4.692 ± 0.098
0.502SerCys: 0.502 ± 0.026
1.959SerAsp: 1.959 ± 0.059
2.612SerGlu: 2.612 ± 0.062
1.818SerPhe: 1.818 ± 0.053
4.148SerGly: 4.148 ± 0.086
0.832SerHis: 0.832 ± 0.038
3.063SerIle: 3.063 ± 0.08
2.556SerLys: 2.556 ± 0.063
5.787SerLeu: 5.787 ± 0.108
1.041SerMet: 1.041 ± 0.036
1.148SerAsn: 1.148 ± 0.044
3.075SerPro: 3.075 ± 0.084
1.362SerGln: 1.362 ± 0.047
3.092SerArg: 3.092 ± 0.067
2.535SerSer: 2.535 ± 0.076
2.353SerThr: 2.353 ± 0.073
4.052SerVal: 4.052 ± 0.089
0.854SerTrp: 0.854 ± 0.039
1.872SerTyr: 1.872 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
5.106ThrAla: 5.106 ± 0.101
0.421ThrCys: 0.421 ± 0.028
1.579ThrAsp: 1.579 ± 0.056
2.165ThrGlu: 2.165 ± 0.058
1.49ThrPhe: 1.49 ± 0.05
3.583ThrGly: 3.583 ± 0.073
0.791ThrHis: 0.791 ± 0.034
2.435ThrIle: 2.435 ± 0.065
1.806ThrLys: 1.806 ± 0.056
4.579ThrLeu: 4.579 ± 0.097
0.759ThrMet: 0.759 ± 0.034
1.096ThrAsn: 1.096 ± 0.053
3.39ThrPro: 3.39 ± 0.08
0.924ThrGln: 0.924 ± 0.043
2.299ThrArg: 2.299 ± 0.059
2.551ThrSer: 2.551 ± 0.067
2.422ThrThr: 2.422 ± 0.07
3.993ThrVal: 3.993 ± 0.088
0.71ThrTrp: 0.71 ± 0.036
1.967ThrTyr: 1.967 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
9.329ValAla: 9.329 ± 0.14
0.65ValCys: 0.65 ± 0.035
4.828ValAsp: 4.828 ± 0.097
8.022ValGlu: 8.022 ± 0.126
3.568ValPhe: 3.568 ± 0.074
6.248ValGly: 6.248 ± 0.095
1.163ValHis: 1.163 ± 0.039
5.863ValIle: 5.863 ± 0.095
6.245ValLys: 6.245 ± 0.106
9.076ValLeu: 9.076 ± 0.136
1.531ValMet: 1.531 ± 0.048
2.583ValAsn: 2.583 ± 0.056
3.655ValPro: 3.655 ± 0.075
1.588ValGln: 1.588 ± 0.046
5.333ValArg: 5.333 ± 0.094
4.109ValSer: 4.109 ± 0.09
3.671ValThr: 3.671 ± 0.081
10.428ValVal: 10.428 ± 0.158
1.089ValTrp: 1.089 ± 0.044
4.226ValTyr: 4.226 ± 0.084
0.0ValXaa: 0.0 ± 0.0
Trp
1.415TrpAla: 1.415 ± 0.049
0.108TrpCys: 0.108 ± 0.014
0.614TrpAsp: 0.614 ± 0.034
0.933TrpGlu: 0.933 ± 0.043
0.469TrpPhe: 0.469 ± 0.031
1.31TrpGly: 1.31 ± 0.049
0.266TrpHis: 0.266 ± 0.023
0.902TrpIle: 0.902 ± 0.035
0.611TrpLys: 0.611 ± 0.03
1.712TrpLeu: 1.712 ± 0.06
0.211TrpMet: 0.211 ± 0.019
0.409TrpAsn: 0.409 ± 0.026
0.652TrpPro: 0.652 ± 0.033
0.347TrpGln: 0.347 ± 0.024
1.541TrpArg: 1.541 ± 0.055
0.763TrpSer: 0.763 ± 0.035
0.58TrpThr: 0.58 ± 0.032
1.018TrpVal: 1.018 ± 0.04
0.267TrpTrp: 0.267 ± 0.022
0.612TrpTyr: 0.612 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.035TyrAla: 4.035 ± 0.074
0.386TyrCys: 0.386 ± 0.028
1.927TyrAsp: 1.927 ± 0.062
2.318TyrGlu: 2.318 ± 0.067
1.651TyrPhe: 1.651 ± 0.056
3.374TyrGly: 3.374 ± 0.069
0.6TyrHis: 0.6 ± 0.03
3.278TyrIle: 3.278 ± 0.072
2.443TyrLys: 2.443 ± 0.069
4.5TyrLeu: 4.5 ± 0.082
0.861TyrMet: 0.861 ± 0.039
1.212TyrAsn: 1.212 ± 0.052
1.675TyrPro: 1.675 ± 0.061
0.889TyrGln: 0.889 ± 0.037
2.535TyrArg: 2.535 ± 0.063
2.112TyrSer: 2.112 ± 0.059
2.191TyrThr: 2.191 ± 0.062
4.551TyrVal: 4.551 ± 0.086
0.588TyrTrp: 0.588 ± 0.035
1.959TyrTyr: 1.959 ± 0.064
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.002XaaAsn: 0.002 ± 0.002
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2590 proteins (654950 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski