Bacteroides coprosuis DSM 18011
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2385 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F3ZRC5|F3ZRC5_9BACE Fumble domain protein OS=Bacteroides coprosuis DSM 18011 OX=679937 GN=Bcop_1742 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.22 KK3 pKa = 10.16 YY4 pKa = 10.18 VCTVCGYY11 pKa = 10.9 VYY13 pKa = 10.63 DD14 pKa = 4.41 PAEE17 pKa = 4.51 GDD19 pKa = 3.56 PDD21 pKa = 4.11 NGIKK25 pKa = 10.36 PGTAFEE31 pKa = 5.13 DD32 pKa = 4.83 LPDD35 pKa = 3.86 DD36 pKa = 4.25 WVCPEE41 pKa = 4.48 CGVGKK46 pKa = 10.47 EE47 pKa = 4.09 DD48 pKa = 5.04 FEE50 pKa = 4.85 PSEE53 pKa = 4.39 DD54 pKa = 3.43
Molecular weight: 5.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.797
IPC2_protein 3.77
IPC_protein 3.694
Toseland 3.49
ProMoST 3.783
Dawson 3.694
Bjellqvist 3.923
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.656
Lehninger 3.617
Nozaki 3.834
DTASelect 4.024
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 0.401
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|F3ZUL3|F3ZUL3_9BACE Xanthine/uracil/vitamin C permease OS=Bacteroides coprosuis DSM 18011 OX=679937 GN=Bcop_0971 PE=4 SV=1
MM1 pKa = 7.66 RR2 pKa = 11.84 RR3 pKa = 11.84 NNAEE7 pKa = 3.86 KK8 pKa = 10.77 VGDD11 pKa = 3.4 ILRR14 pKa = 11.84 RR15 pKa = 11.84 FLRR18 pKa = 11.84 QEE20 pKa = 4.0 SLEE23 pKa = 4.25 SPLNEE28 pKa = 4.88 QRR30 pKa = 11.84 LLDD33 pKa = 4.12 LWPKK37 pKa = 10.51 VLGPTMARR45 pKa = 11.84 YY46 pKa = 6.83 TRR48 pKa = 11.84 KK49 pKa = 9.81 IYY51 pKa = 10.14 IHH53 pKa = 5.77 NQILYY58 pKa = 9.66 VHH60 pKa = 6.64 LTSAALRR67 pKa = 11.84 QEE69 pKa = 4.23 LMMGRR74 pKa = 11.84 EE75 pKa = 3.89 LLVRR79 pKa = 11.84 KK80 pKa = 9.95 LNQEE84 pKa = 3.48 VGANVITNIIFRR96 pKa = 4.32
Molecular weight: 11.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.736
IPC_protein 10.716
Toseland 10.73
ProMoST 10.921
Dawson 10.833
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 10.921
Grimsley 10.891
Solomon 10.994
Lehninger 10.95
Nozaki 10.701
DTASelect 10.584
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.76
Patrickios 10.687
IPC_peptide 10.994
IPC2_peptide 9.502
IPC2.peptide.svr19 8.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2385
0
2385
839534
31
1932
352.0
39.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.321 ± 0.043
0.982 ± 0.018
5.615 ± 0.033
6.758 ± 0.054
4.749 ± 0.032
6.293 ± 0.05
1.912 ± 0.021
7.94 ± 0.046
7.582 ± 0.044
9.511 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.464 ± 0.022
5.448 ± 0.042
3.522 ± 0.025
3.541 ± 0.024
3.886 ± 0.032
6.49 ± 0.035
5.347 ± 0.032
6.19 ± 0.041
1.102 ± 0.02
4.346 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here