Desulfosporosinus sp. Tol-M

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfosporosinus; unclassified Desulfosporosinus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2TL99|A0A0A2TL99_9FIRM Orotidine 5'-phosphate decarboxylase OS=Desulfosporosinus sp. Tol-M OX=1536651 GN=pyrF PE=3 SV=1
MM1 pKa = 6.99FAEE4 pKa = 4.26VDD6 pKa = 3.08KK7 pKa = 10.88DD8 pKa = 3.48ACIGCGACPDD18 pKa = 3.4ICPEE22 pKa = 3.82IFKK25 pKa = 11.1LEE27 pKa = 3.99EE28 pKa = 4.71DD29 pKa = 4.24EE30 pKa = 6.35LATAYY35 pKa = 10.24NNPVPRR41 pKa = 11.84DD42 pKa = 3.63FEE44 pKa = 4.36EE45 pKa = 4.35STKK48 pKa = 10.47EE49 pKa = 3.81AAEE52 pKa = 4.21SCPTEE57 pKa = 4.77AIHH60 pKa = 7.98LEE62 pKa = 4.24EE63 pKa = 4.35

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2TH81|A0A0A2TH81_9FIRM Bac_transf domain-containing protein OS=Desulfosporosinus sp. Tol-M OX=1536651 GN=JT05_11160 PE=3 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84VLAFLLAMILLLLIGLALKK21 pKa = 10.95AEE23 pKa = 3.86VDD25 pKa = 3.81FRR27 pKa = 11.84YY28 pKa = 10.19RR29 pKa = 11.84RR30 pKa = 11.84IEE32 pKa = 4.05EE33 pKa = 3.99EE34 pKa = 3.78DD35 pKa = 3.62HH36 pKa = 8.35IEE38 pKa = 3.44IDD40 pKa = 3.52FRR42 pKa = 11.84AFHH45 pKa = 6.18GLWHH49 pKa = 6.55IQYY52 pKa = 9.98QIPTLQLEE60 pKa = 4.63WEE62 pKa = 4.5KK63 pKa = 11.22GPEE66 pKa = 3.84VEE68 pKa = 4.44MEE70 pKa = 3.89QVAITKK76 pKa = 10.35GGTRR80 pKa = 11.84QTTKK84 pKa = 10.26KK85 pKa = 10.64ARR87 pKa = 11.84IRR89 pKa = 11.84YY90 pKa = 8.35IRR92 pKa = 11.84RR93 pKa = 11.84GWLSRR98 pKa = 11.84FWPNVPRR105 pKa = 11.84LLRR108 pKa = 11.84HH109 pKa = 6.39IYY111 pKa = 9.59RR112 pKa = 11.84VKK114 pKa = 8.8QQFYY118 pKa = 10.96RR119 pKa = 11.84GIHH122 pKa = 5.8CKK124 pKa = 10.28AINWRR129 pKa = 11.84IEE131 pKa = 3.88IGCQDD136 pKa = 3.56AAQTAIAAGAFWTMFGFALSRR157 pKa = 11.84LYY159 pKa = 10.68RR160 pKa = 11.84QVTVEE165 pKa = 4.01VSSPALVVIPRR176 pKa = 11.84FKK178 pKa = 10.82KK179 pKa = 10.51AGFLCDD185 pKa = 4.04LQCIFQLRR193 pKa = 11.84ISHH196 pKa = 7.02IIFGGFNVLRR206 pKa = 11.84IIRR209 pKa = 11.84RR210 pKa = 11.84GIRR213 pKa = 11.84GG214 pKa = 3.22

Molecular weight: 25.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2539

0

2539

749546

31

2876

295.2

32.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.708 ± 0.048

1.145 ± 0.022

5.065 ± 0.038

6.945 ± 0.05

4.009 ± 0.035

7.367 ± 0.044

1.885 ± 0.024

7.362 ± 0.048

6.061 ± 0.044

10.292 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.022

4.081 ± 0.037

3.989 ± 0.033

3.796 ± 0.032

5.129 ± 0.038

5.799 ± 0.034

5.377 ± 0.041

7.069 ± 0.048

1.06 ± 0.017

3.337 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski