Amino acid dipepetide frequency for Desulfosporosinus sp. Tol-M

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.733AlaAla: 6.733 ± 0.113	0.937AlaCys: 0.937 ± 0.037	3.99AlaAsp: 3.99 ± 0.077	5.459AlaGlu: 5.459 ± 0.1	2.951AlaPhe: 2.951 ± 0.063	6.098AlaGly: 6.098 ± 0.103	1.397AlaHis: 1.397 ± 0.045	5.383AlaIle: 5.383 ± 0.088	4.635AlaLys: 4.635 ± 0.083	8.573AlaLeu: 8.573 ± 0.143	2.147AlaMet: 2.147 ± 0.059	2.892AlaAsn: 2.892 ± 0.067	2.255AlaPro: 2.255 ± 0.057	3.118AlaGln: 3.118 ± 0.074	3.905AlaArg: 3.905 ± 0.067	3.872AlaSer: 3.872 ± 0.073	3.593AlaThr: 3.593 ± 0.078	5.773AlaVal: 5.773 ± 0.1	0.815AlaTrp: 0.815 ± 0.036	2.547AlaTyr: 2.547 ± 0.065	0.004AlaXaa: 0.004 ± 0.002
Cys	0.815CysAla: 0.815 ± 0.031	0.192CysCys: 0.192 ± 0.018	0.518CysAsp: 0.518 ± 0.028	0.679CysGlu: 0.679 ± 0.027	0.442CysPhe: 0.442 ± 0.029	1.199CysGly: 1.199 ± 0.044	0.282CysHis: 0.282 ± 0.019	0.752CysIle: 0.752 ± 0.035	0.548CysLys: 0.548 ± 0.027	1.063CysLeu: 1.063 ± 0.036	0.221CysMet: 0.221 ± 0.016	0.422CysAsn: 0.422 ± 0.026	0.614CysPro: 0.614 ± 0.037	0.454CysGln: 0.454 ± 0.024	0.62CysArg: 0.62 ± 0.026	0.778CysSer: 0.778 ± 0.033	0.612CysThr: 0.612 ± 0.028	0.732CysVal: 0.732 ± 0.031	0.113CysTrp: 0.113 ± 0.012	0.388CysTyr: 0.388 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.474AspAla: 3.474 ± 0.072	0.596AspCys: 0.596 ± 0.031	2.316AspAsp: 2.316 ± 0.059	3.673AspGlu: 3.673 ± 0.068	2.456AspPhe: 2.456 ± 0.055	3.494AspGly: 3.494 ± 0.072	0.927AspHis: 0.927 ± 0.036	3.993AspIle: 3.993 ± 0.085	2.962AspLys: 2.962 ± 0.07	5.581AspLeu: 5.581 ± 0.085	1.29AspMet: 1.29 ± 0.045	1.937AspAsn: 1.937 ± 0.058	2.265AspPro: 2.265 ± 0.061	1.75AspGln: 1.75 ± 0.058	2.494AspArg: 2.494 ± 0.063	2.791AspSer: 2.791 ± 0.07	2.464AspThr: 2.464 ± 0.074	3.517AspVal: 3.517 ± 0.076	0.606AspTrp: 0.606 ± 0.03	2.067AspTyr: 2.067 ± 0.06	0.001AspXaa: 0.001 ± 0.001
Glu	5.426GluAla: 5.426 ± 0.1	0.659GluCys: 0.659 ± 0.03	3.378GluAsp: 3.378 ± 0.068	5.462GluGlu: 5.462 ± 0.118	2.54GluPhe: 2.54 ± 0.055	4.445GluGly: 4.445 ± 0.084	1.317GluHis: 1.317 ± 0.043	5.406GluIle: 5.406 ± 0.098	5.044GluLys: 5.044 ± 0.097	7.162GluLeu: 7.162 ± 0.119	1.943GluMet: 1.943 ± 0.058	3.109GluAsn: 3.109 ± 0.067	1.909GluPro: 1.909 ± 0.055	3.102GluGln: 3.102 ± 0.067	4.016GluArg: 4.016 ± 0.077	3.325GluSer: 3.325 ± 0.063	3.389GluThr: 3.389 ± 0.069	4.96GluVal: 4.96 ± 0.089	0.703GluTrp: 0.703 ± 0.033	2.149GluTyr: 2.149 ± 0.063	0.007GluXaa: 0.007 ± 0.004
Phe	2.927PheAla: 2.927 ± 0.063	0.582PheCys: 0.582 ± 0.026	2.143PheAsp: 2.143 ± 0.058	2.459PheGlu: 2.459 ± 0.058	1.816PhePhe: 1.816 ± 0.055	3.122PheGly: 3.122 ± 0.07	0.751PheHis: 0.751 ± 0.032	2.903PheIle: 2.903 ± 0.072	2.069PheLys: 2.069 ± 0.055	4.048PheLeu: 4.048 ± 0.083	0.949PheMet: 0.949 ± 0.037	1.698PheAsn: 1.698 ± 0.049	1.682PhePro: 1.682 ± 0.048	1.38PheGln: 1.38 ± 0.044	1.905PheArg: 1.905 ± 0.049	2.908PheSer: 2.908 ± 0.059	2.239PheThr: 2.239 ± 0.061	2.555PheVal: 2.555 ± 0.066	0.499PheTrp: 0.499 ± 0.032	1.45PheTyr: 1.45 ± 0.045	0.001PheXaa: 0.001 ± 0.001
Gly	5.164GlyAla: 5.164 ± 0.098	1.025GlyCys: 1.025 ± 0.042	3.419GlyAsp: 3.419 ± 0.083	4.711GlyGlu: 4.711 ± 0.093	3.171GlyPhe: 3.171 ± 0.068	5.307GlyGly: 5.307 ± 0.1	1.357GlyHis: 1.357 ± 0.046	6.361GlyIle: 6.361 ± 0.097	5.132GlyLys: 5.132 ± 0.077	7.412GlyLeu: 7.412 ± 0.113	2.096GlyMet: 2.096 ± 0.056	2.755GlyAsn: 2.755 ± 0.069	1.876GlyPro: 1.876 ± 0.057	2.722GlyGln: 2.722 ± 0.061	3.693GlyArg: 3.693 ± 0.085	4.205GlySer: 4.205 ± 0.079	4.223GlyThr: 4.223 ± 0.091	5.489GlyVal: 5.489 ± 0.098	0.815GlyTrp: 0.815 ± 0.036	2.732GlyTyr: 2.732 ± 0.063	0.001GlyXaa: 0.001 ± 0.001
His	1.282HisAla: 1.282 ± 0.049	0.291HisCys: 0.291 ± 0.021	0.883HisAsp: 0.883 ± 0.034	1.077HisGlu: 1.077 ± 0.037	0.89HisPhe: 0.89 ± 0.037	1.397HisGly: 1.397 ± 0.044	0.48HisHis: 0.48 ± 0.025	1.267HisIle: 1.267 ± 0.04	0.959HisLys: 0.959 ± 0.039	1.973HisLeu: 1.973 ± 0.053	0.412HisMet: 0.412 ± 0.023	0.744HisAsn: 0.744 ± 0.034	1.19HisPro: 1.19 ± 0.044	0.723HisGln: 0.723 ± 0.035	0.967HisArg: 0.967 ± 0.039	1.113HisSer: 1.113 ± 0.039	0.969HisThr: 0.969 ± 0.034	1.206HisVal: 1.206 ± 0.038	0.259HisTrp: 0.259 ± 0.024	0.767HisTyr: 0.767 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.933IleAla: 5.933 ± 0.101	0.847IleCys: 0.847 ± 0.031	3.857IleAsp: 3.857 ± 0.084	4.895IleGlu: 4.895 ± 0.093	2.871IlePhe: 2.871 ± 0.086	5.371IleGly: 5.371 ± 0.099	1.386IleHis: 1.386 ± 0.046	5.257IleIle: 5.257 ± 0.098	4.224IleLys: 4.224 ± 0.078	7.497IleLeu: 7.497 ± 0.121	1.734IleMet: 1.734 ± 0.048	3.083IleAsn: 3.083 ± 0.065	3.629IlePro: 3.629 ± 0.074	2.635IleGln: 2.635 ± 0.064	3.688IleArg: 3.688 ± 0.071	4.799IleSer: 4.799 ± 0.081	4.159IleThr: 4.159 ± 0.075	4.86IleVal: 4.86 ± 0.088	0.616IleTrp: 0.616 ± 0.028	2.276IleTyr: 2.276 ± 0.064	0.003IleXaa: 0.003 ± 0.002
Lys	4.763LysAla: 4.763 ± 0.085	0.522LysCys: 0.522 ± 0.03	3.275LysAsp: 3.275 ± 0.072	4.653LysGlu: 4.653 ± 0.084	1.931LysPhe: 1.931 ± 0.053	4.072LysGly: 4.072 ± 0.084	1.119LysHis: 1.119 ± 0.042	4.512LysIle: 4.512 ± 0.083	4.078LysLys: 4.078 ± 0.084	5.569LysLeu: 5.569 ± 0.082	1.665LysMet: 1.665 ± 0.046	2.714LysAsn: 2.714 ± 0.066	2.277LysPro: 2.277 ± 0.063	2.215LysGln: 2.215 ± 0.063	3.267LysArg: 3.267 ± 0.086	3.25LysSer: 3.25 ± 0.072	3.538LysThr: 3.538 ± 0.069	4.463LysVal: 4.463 ± 0.076	0.58LysTrp: 0.58 ± 0.032	2.147LysTyr: 2.147 ± 0.063	0.001LysXaa: 0.001 ± 0.001
Leu	8.821LeuAla: 8.821 ± 0.129	1.113LeuCys: 1.113 ± 0.038	5.33LeuAsp: 5.33 ± 0.085	7.254LeuGlu: 7.254 ± 0.116	3.8LeuPhe: 3.8 ± 0.083	7.723LeuGly: 7.723 ± 0.12	1.81LeuHis: 1.81 ± 0.049	7.131LeuIle: 7.131 ± 0.121	6.491LeuLys: 6.491 ± 0.102	10.224LeuLeu: 10.224 ± 0.189	2.405LeuMet: 2.405 ± 0.07	4.175LeuAsn: 4.175 ± 0.081	4.587LeuPro: 4.587 ± 0.078	3.758LeuGln: 3.758 ± 0.076	5.487LeuArg: 5.487 ± 0.092	6.451LeuSer: 6.451 ± 0.119	5.985LeuThr: 5.985 ± 0.097	6.648LeuVal: 6.648 ± 0.106	0.982LeuTrp: 0.982 ± 0.036	2.746LeuTyr: 2.746 ± 0.06	0.001LeuXaa: 0.001 ± 0.001
Met	2.279MetAla: 2.279 ± 0.056	0.211MetCys: 0.211 ± 0.018	1.444MetAsp: 1.444 ± 0.043	1.73MetGlu: 1.73 ± 0.046	0.886MetPhe: 0.886 ± 0.041	1.916MetGly: 1.916 ± 0.058	0.483MetHis: 0.483 ± 0.026	1.674MetIle: 1.674 ± 0.055	1.72MetLys: 1.72 ± 0.048	2.44MetLeu: 2.44 ± 0.062	0.626MetMet: 0.626 ± 0.03	1.205MetAsn: 1.205 ± 0.043	1.078MetPro: 1.078 ± 0.035	0.865MetGln: 0.865 ± 0.034	1.285MetArg: 1.285 ± 0.038	1.425MetSer: 1.425 ± 0.046	1.457MetThr: 1.457 ± 0.049	1.754MetVal: 1.754 ± 0.049	0.172MetTrp: 0.172 ± 0.015	0.563MetTyr: 0.563 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.867AsnAla: 2.867 ± 0.066	0.486AsnCys: 0.486 ± 0.027	1.868AsnAsp: 1.868 ± 0.051	2.399AsnGlu: 2.399 ± 0.058	1.62AsnPhe: 1.62 ± 0.04	2.871AsnGly: 2.871 ± 0.067	0.779AsnHis: 0.779 ± 0.033	3.174AsnIle: 3.174 ± 0.066	2.341AsnLys: 2.341 ± 0.055	4.056AsnLeu: 4.056 ± 0.08	1.033AsnMet: 1.033 ± 0.032	1.878AsnAsn: 1.878 ± 0.055	2.308AsnPro: 2.308 ± 0.059	1.569AsnGln: 1.569 ± 0.05	2.051AsnArg: 2.051 ± 0.054	2.49AsnSer: 2.49 ± 0.061	2.167AsnThr: 2.167 ± 0.063	2.815AsnVal: 2.815 ± 0.065	0.454AsnTrp: 0.454 ± 0.025	1.586AsnTyr: 1.586 ± 0.052	0.001AsnXaa: 0.001 ± 0.001
Pro	2.843ProAla: 2.843 ± 0.068	0.412ProCys: 0.412 ± 0.024	2.351ProAsp: 2.351 ± 0.062	3.498ProGlu: 3.498 ± 0.073	1.776ProPhe: 1.776 ± 0.048	3.019ProGly: 3.019 ± 0.073	0.786ProHis: 0.786 ± 0.035	2.652ProIle: 2.652 ± 0.057	2.132ProLys: 2.132 ± 0.055	4.03ProLeu: 4.03 ± 0.076	0.906ProMet: 0.906 ± 0.033	1.6ProAsn: 1.6 ± 0.047	1.377ProPro: 1.377 ± 0.052	1.506ProGln: 1.506 ± 0.04	1.629ProArg: 1.629 ± 0.052	2.271ProSer: 2.271 ± 0.053	2.205ProThr: 2.205 ± 0.054	3.054ProVal: 3.054 ± 0.062	0.478ProTrp: 0.478 ± 0.026	1.358ProTyr: 1.358 ± 0.041	0.005ProXaa: 0.005 ± 0.003
Gln	3.414GlnAla: 3.414 ± 0.077	0.346GlnCys: 0.346 ± 0.022	1.86GlnAsp: 1.86 ± 0.056	2.988GlnGlu: 2.988 ± 0.077	1.287GlnPhe: 1.287 ± 0.037	2.838GlnGly: 2.838 ± 0.062	0.716GlnHis: 0.716 ± 0.037	2.736GlnIle: 2.736 ± 0.057	2.488GlnLys: 2.488 ± 0.076	3.626GlnLeu: 3.626 ± 0.076	1.051GlnMet: 1.051 ± 0.038	1.506GlnAsn: 1.506 ± 0.049	1.275GlnPro: 1.275 ± 0.037	1.428GlnGln: 1.428 ± 0.046	1.925GlnArg: 1.925 ± 0.046	1.989GlnSer: 1.989 ± 0.051	2.087GlnThr: 2.087 ± 0.055	2.887GlnVal: 2.887 ± 0.065	0.386GlnTrp: 0.386 ± 0.026	1.126GlnTyr: 1.126 ± 0.038	0.003GlnXaa: 0.003 ± 0.002
Arg	3.35ArgAla: 3.35 ± 0.065	0.566ArgCys: 0.566 ± 0.029	2.519ArgAsp: 2.519 ± 0.061	3.964ArgGlu: 3.964 ± 0.083	2.243ArgPhe: 2.243 ± 0.054	3.161ArgGly: 3.161 ± 0.071	1.023ArgHis: 1.023 ± 0.041	4.038ArgIle: 4.038 ± 0.07	3.273ArgLys: 3.273 ± 0.064	5.474ArgLeu: 5.474 ± 0.091	1.397ArgMet: 1.397 ± 0.047	2.08ArgAsn: 2.08 ± 0.057	1.821ArgPro: 1.821 ± 0.057	2.239ArgGln: 2.239 ± 0.055	2.958ArgArg: 2.958 ± 0.07	2.579ArgSer: 2.579 ± 0.065	2.564ArgThr: 2.564 ± 0.06	3.601ArgVal: 3.601 ± 0.061	0.626ArgTrp: 0.626 ± 0.03	1.809ArgTyr: 1.809 ± 0.047	0.003ArgXaa: 0.003 ± 0.002
Ser	4.248SerAla: 4.248 ± 0.079	0.635SerCys: 0.635 ± 0.027	2.778SerAsp: 2.778 ± 0.059	3.736SerGlu: 3.736 ± 0.062	2.472SerPhe: 2.472 ± 0.054	4.936SerGly: 4.936 ± 0.094	1.042SerHis: 1.042 ± 0.039	4.205SerIle: 4.205 ± 0.084	3.207SerLys: 3.207 ± 0.06	6.045SerLeu: 6.045 ± 0.106	1.49SerMet: 1.49 ± 0.04	2.256SerAsn: 2.256 ± 0.064	2.49SerPro: 2.49 ± 0.056	2.107SerGln: 2.107 ± 0.057	2.987SerArg: 2.987 ± 0.063	3.704SerSer: 3.704 ± 0.088	2.954SerThr: 2.954 ± 0.078	4.123SerVal: 4.123 ± 0.09	0.619SerTrp: 0.619 ± 0.032	1.957SerTyr: 1.957 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	4.315ThrAla: 4.315 ± 0.078	0.554ThrCys: 0.554 ± 0.027	2.732ThrAsp: 2.732 ± 0.064	3.402ThrGlu: 3.402 ± 0.071	2.143ThrPhe: 2.143 ± 0.052	4.665ThrGly: 4.665 ± 0.081	1.03ThrHis: 1.03 ± 0.034	4.0ThrIle: 4.0 ± 0.074	2.822ThrLys: 2.822 ± 0.073	5.766ThrLeu: 5.766 ± 0.087	1.195ThrMet: 1.195 ± 0.04	2.004ThrAsn: 2.004 ± 0.05	2.586ThrPro: 2.586 ± 0.063	1.781ThrGln: 1.781 ± 0.051	2.291ThrArg: 2.291 ± 0.061	2.975ThrSer: 2.975 ± 0.084	3.003ThrThr: 3.003 ± 0.085	4.177ThrVal: 4.177 ± 0.088	0.591ThrTrp: 0.591 ± 0.027	1.737ThrTyr: 1.737 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	5.118ValAla: 5.118 ± 0.091	0.869ValCys: 0.869 ± 0.035	3.685ValAsp: 3.685 ± 0.067	4.671ValGlu: 4.671 ± 0.081	2.836ValPhe: 2.836 ± 0.067	4.836ValGly: 4.836 ± 0.091	1.247ValHis: 1.247 ± 0.042	5.299ValIle: 5.299 ± 0.09	4.191ValLys: 4.191 ± 0.075	7.683ValLeu: 7.683 ± 0.121	1.773ValMet: 1.773 ± 0.047	2.818ValAsn: 2.818 ± 0.062	2.836ValPro: 2.836 ± 0.063	2.542ValGln: 2.542 ± 0.058	3.592ValArg: 3.592 ± 0.071	4.441ValSer: 4.441 ± 0.083	3.9ValThr: 3.9 ± 0.093	5.322ValVal: 5.322 ± 0.102	0.752ValTrp: 0.752 ± 0.031	2.276ValTyr: 2.276 ± 0.058	0.001ValXaa: 0.001 ± 0.001
Trp	0.756TrpAla: 0.756 ± 0.032	0.123TrpCys: 0.123 ± 0.014	0.578TrpAsp: 0.578 ± 0.03	0.715TrpGlu: 0.715 ± 0.03	0.4TrpPhe: 0.4 ± 0.022	0.75TrpGly: 0.75 ± 0.029	0.221TrpHis: 0.221 ± 0.016	0.692TrpIle: 0.692 ± 0.029	0.591TrpLys: 0.591 ± 0.029	1.141TrpLeu: 1.141 ± 0.043	0.259TrpMet: 0.259 ± 0.017	0.506TrpAsn: 0.506 ± 0.024	0.359TrpPro: 0.359 ± 0.024	0.61TrpGln: 0.61 ± 0.029	0.627TrpArg: 0.627 ± 0.032	0.6TrpSer: 0.6 ± 0.032	0.499TrpThr: 0.499 ± 0.029	0.723TrpVal: 0.723 ± 0.033	0.159TrpTrp: 0.159 ± 0.015	0.291TrpTyr: 0.291 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.544TyrAla: 2.544 ± 0.068	0.483TyrCys: 0.483 ± 0.029	1.73TyrAsp: 1.73 ± 0.057	2.023TyrGlu: 2.023 ± 0.05	1.556TyrPhe: 1.556 ± 0.05	2.441TyrGly: 2.441 ± 0.055	0.694TyrHis: 0.694 ± 0.031	2.18TyrIle: 2.18 ± 0.058	1.702TyrLys: 1.702 ± 0.052	3.553TyrLeu: 3.553 ± 0.072	0.616TyrMet: 0.616 ± 0.028	1.43TyrAsn: 1.43 ± 0.046	1.474TyrPro: 1.474 ± 0.048	1.462TyrGln: 1.462 ± 0.047	1.898TyrArg: 1.898 ± 0.049	2.024TyrSer: 2.024 ± 0.056	1.761TyrThr: 1.761 ± 0.053	2.027TyrVal: 2.027 ± 0.051	0.375TyrTrp: 0.375 ± 0.023	1.392TyrTyr: 1.392 ± 0.05	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.003	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.005XaaLeu: 0.005 ± 0.004	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.003XaaThr: 0.003 ± 0.002	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.003XaaTyr: 0.003 ± 0.002	0.009XaaXaa: 0.009 ± 0.006

Statistics based on 2539 proteins (749546 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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