Oleiphilus sp. HI0079

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oleiphilaceae; Oleiphilus; unclassified Oleiphilus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A657BAF4|A0A657BAF4_9GAMM Uncharacterized protein OS=Oleiphilus sp. HI0079 OX=1822254 GN=A3750_11315 PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.11LKK4 pKa = 9.87PQPGWSKK11 pKa = 11.02LSLAVAVAGALSLGGCKK28 pKa = 7.77VTIKK32 pKa = 10.61ADD34 pKa = 3.4EE35 pKa = 4.9GEE37 pKa = 4.17VSDD40 pKa = 5.09IVDD43 pKa = 3.8SVTDD47 pKa = 3.61NAVCRR52 pKa = 11.84DD53 pKa = 3.5DD54 pKa = 5.65DD55 pKa = 4.55GNKK58 pKa = 9.35VEE60 pKa = 5.3CGNSDD65 pKa = 3.5GGSATVLSASGYY77 pKa = 8.61EE78 pKa = 3.98IPEE81 pKa = 4.04DD82 pKa = 4.54AIFVAADD89 pKa = 3.36ARR91 pKa = 11.84DD92 pKa = 3.69GDD94 pKa = 5.26DD95 pKa = 3.28ITDD98 pKa = 5.36AINLALFEE106 pKa = 4.48VPAGSTIGLPKK117 pKa = 10.21GTFKK121 pKa = 10.93VSNSIIVYY129 pKa = 8.77NANDD133 pKa = 3.68ITLMGYY139 pKa = 10.07GINDD143 pKa = 3.64TVLDD147 pKa = 4.09FDD149 pKa = 5.29GSSGDD154 pKa = 3.34DD155 pKa = 3.19GFRR158 pKa = 11.84FEE160 pKa = 5.01GGSDD164 pKa = 3.52LMVRR168 pKa = 11.84DD169 pKa = 4.02LGVYY173 pKa = 7.63DD174 pKa = 3.83TNKK177 pKa = 10.65NGIKK181 pKa = 9.29TVGSNGVYY189 pKa = 9.92FSHH192 pKa = 6.84VAAVWTVGRR201 pKa = 11.84LSDD204 pKa = 3.82EE205 pKa = 4.21TPKK208 pKa = 10.87RR209 pKa = 11.84LLADD213 pKa = 3.39SPGAYY218 pKa = 9.75GLYY221 pKa = 9.73PVNSKK226 pKa = 11.15NIVIEE231 pKa = 4.21DD232 pKa = 3.83TFSCGSRR239 pKa = 11.84DD240 pKa = 3.17AGIYY244 pKa = 9.57VGQSEE249 pKa = 4.65DD250 pKa = 3.5VVVRR254 pKa = 11.84NNLVEE259 pKa = 4.17YY260 pKa = 9.7NVAGIEE266 pKa = 4.46IEE268 pKa = 4.13NTEE271 pKa = 4.15NADD274 pKa = 3.45VYY276 pKa = 11.8NNDD279 pKa = 3.51VYY281 pKa = 11.72DD282 pKa = 4.07NTAGWSPVTRR292 pKa = 11.84NINIHH297 pKa = 6.01NNSLSQNSTDD307 pKa = 3.57PGGKK311 pKa = 9.43LLEE314 pKa = 4.35EE315 pKa = 4.71AGVIAGYY322 pKa = 8.97EE323 pKa = 4.04AYY325 pKa = 10.27YY326 pKa = 10.68GAGSFPDD333 pKa = 3.38ILYY336 pKa = 10.88GGIGEE341 pKa = 4.73LLANAGEE348 pKa = 4.15LAPLGIAPYY357 pKa = 10.42AADD360 pKa = 4.25GSDD363 pKa = 3.87NVCEE367 pKa = 4.27TNNFEE372 pKa = 4.36AQQGAADD379 pKa = 3.98EE380 pKa = 4.67ASVGLVYY387 pKa = 9.32GTNPMDD393 pKa = 4.46PNNWVWDD400 pKa = 4.09GTPGASNILGPQATLSRR417 pKa = 11.84EE418 pKa = 4.06LLSANANMNCSLPRR432 pKa = 11.84LAGSSVVFRR441 pKa = 11.84GEE443 pKa = 3.92EE444 pKa = 4.05FGCNSDD450 pKa = 5.6DD451 pKa = 4.08LDD453 pKa = 4.54EE454 pKa = 4.59YY455 pKa = 11.62ACSLL459 pKa = 3.82

Molecular weight: 48.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A657BC37|A0A657BC37_9GAMM Nitrate ABC transporter ATP-binding protein OS=Oleiphilus sp. HI0079 OX=1822254 GN=A3750_09320 PE=4 SV=1
MM1 pKa = 7.58GIEE4 pKa = 3.84TAKK7 pKa = 10.58AAQHH11 pKa = 5.73PKK13 pKa = 8.33MRR15 pKa = 11.84RR16 pKa = 11.84YY17 pKa = 7.93DD18 pKa = 3.63TRR20 pKa = 11.84PSNVFHH26 pKa = 6.78LSSRR30 pKa = 11.84SMLRR34 pKa = 11.84LHH36 pKa = 7.06GMNLNFGEE44 pKa = 4.41LPRR47 pKa = 11.84FNLQIRR53 pKa = 11.84VFNFTISNFLLDD65 pKa = 3.66WRR67 pKa = 11.84AHH69 pKa = 5.68EE70 pKa = 4.33SSEE73 pKa = 4.14

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3311

0

3311

1015081

51

2284

306.6

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.092 ± 0.04

1.016 ± 0.012

5.826 ± 0.038

6.808 ± 0.042

4.153 ± 0.024

6.955 ± 0.044

2.161 ± 0.022

6.006 ± 0.033

5.041 ± 0.038

10.404 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.021

3.859 ± 0.029

3.916 ± 0.026

4.093 ± 0.028

5.064 ± 0.033

7.108 ± 0.036

4.971 ± 0.037

7.027 ± 0.039

1.121 ± 0.015

2.909 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski