Salmonella virus BTP1
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R3Y5R6|A0A1R3Y5R6_9CAUD Capsid scaffolding protein OS=Salmonella virus BTP1 OX=1934252 GN=BTP1_004 PE=4 SV=1
MM1 pKa = 7.29 LHH3 pKa = 7.3 DD4 pKa = 4.58 FDD6 pKa = 7.13 DD7 pKa = 5.13 DD8 pKa = 3.82 EE9 pKa = 5.6 FIALISPEE17 pKa = 3.74 IEE19 pKa = 3.94 EE20 pKa = 4.61 EE21 pKa = 4.24 VEE23 pKa = 4.03 QQINLAAEE31 pKa = 4.07 RR32 pKa = 11.84 QNPVISWDD40 pKa = 3.48 EE41 pKa = 3.67 FAGYY45 pKa = 10.62 YY46 pKa = 9.62 SS47 pKa = 3.45
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.905
IPC2_protein 3.605
IPC_protein 3.465
Toseland 3.3
ProMoST 3.656
Dawson 3.465
Bjellqvist 3.63
Wikipedia 3.401
Rodwell 3.325
Grimsley 3.236
Solomon 3.401
Lehninger 3.35
Nozaki 3.617
DTASelect 3.706
Thurlkill 3.389
EMBOSS 3.414
Sillero 3.592
Patrickios 1.761
IPC_peptide 3.401
IPC2_peptide 3.554
IPC2.peptide.svr19 3.65
Protein with the highest isoelectric point:
>tr|A0A1R3Y5T6|A0A1R3Y5T6_9CAUD Transcriptional activator OS=Salmonella virus BTP1 OX=1934252 GN=c2 PE=4 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 QRR5 pKa = 11.84 RR6 pKa = 11.84 SITDD10 pKa = 4.1 IICEE14 pKa = 3.96 NCKK17 pKa = 10.21 YY18 pKa = 10.77 LPTKK22 pKa = 10.48 RR23 pKa = 11.84 SRR25 pKa = 11.84 NKK27 pKa = 9.55 PKK29 pKa = 10.29 PIPKK33 pKa = 9.96 EE34 pKa = 3.93 SDD36 pKa = 3.19 VKK38 pKa = 9.6 TFNYY42 pKa = 7.51 TAHH45 pKa = 6.73 LWDD48 pKa = 3.61 IRR50 pKa = 11.84 WLRR53 pKa = 11.84 EE54 pKa = 3.55 RR55 pKa = 11.84 ARR57 pKa = 11.84 KK58 pKa = 7.22 TRR60 pKa = 3.32
Molecular weight: 7.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.765
IPC_protein 10.584
Toseland 10.935
ProMoST 10.994
Dawson 10.994
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 11.257
Grimsley 11.038
Solomon 11.169
Lehninger 11.125
Nozaki 10.921
DTASelect 10.716
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.935
Patrickios 11.008
IPC_peptide 11.169
IPC2_peptide 9.78
IPC2.peptide.svr19 8.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
12236
47
725
200.6
22.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.276 ± 0.528
1.226 ± 0.154
6.056 ± 0.263
6.309 ± 0.378
3.228 ± 0.212
7.159 ± 0.401
1.757 ± 0.201
6.415 ± 0.312
6.154 ± 0.331
7.511 ± 0.284
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.803 ± 0.195
4.642 ± 0.233
3.972 ± 0.211
4.732 ± 0.499
5.901 ± 0.348
6.44 ± 0.388
5.239 ± 0.293
6.097 ± 0.269
1.667 ± 0.188
3.416 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here