Chrysanthemum virus R
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1JI50|A0A2P1JI50_9VIRU Triple gene block protein 1 OS=Chrysanthemum virus R OX=2116736 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.86 DD2 pKa = 4.15 VLVNLAKK9 pKa = 10.71 NFGFHH14 pKa = 6.76 RR15 pKa = 11.84 VCTTLNKK22 pKa = 10.11 PIVFHH27 pKa = 6.23 CVPGAGKK34 pKa = 8.44 STLIRR39 pKa = 11.84 DD40 pKa = 4.02 CCRR43 pKa = 11.84 ADD45 pKa = 3.25 SRR47 pKa = 11.84 FVAVTLGKK55 pKa = 10.28 EE56 pKa = 4.26 DD57 pKa = 3.85 EE58 pKa = 4.45 RR59 pKa = 11.84 DD60 pKa = 3.42 LTGVGITKK68 pKa = 10.5 YY69 pKa = 10.59 SGTIPDD75 pKa = 4.24 CEE77 pKa = 4.1 YY78 pKa = 10.75 PLLDD82 pKa = 4.51 EE83 pKa = 4.34 YY84 pKa = 11.29 TLAEE88 pKa = 4.3 SLSEE92 pKa = 3.77 GWFAIFGDD100 pKa = 4.95 PIQAANRR107 pKa = 11.84 VVLRR111 pKa = 11.84 AHH113 pKa = 6.28 FTCNLSRR120 pKa = 11.84 RR121 pKa = 11.84 FGKK124 pKa = 8.65 CTAQFLRR131 pKa = 11.84 EE132 pKa = 4.03 LGFDD136 pKa = 3.52 VVAEE140 pKa = 4.47 GEE142 pKa = 4.3 DD143 pKa = 3.65 TVQISGIYY151 pKa = 10.14 VEE153 pKa = 5.11 DD154 pKa = 4.01 PRR156 pKa = 11.84 DD157 pKa = 3.56 KK158 pKa = 10.4 IIYY161 pKa = 8.49 YY162 pKa = 9.61 EE163 pKa = 4.16 PEE165 pKa = 4.0 VGCLLRR171 pKa = 11.84 SHH173 pKa = 6.32 SVEE176 pKa = 3.63 ASHH179 pKa = 5.9 ITEE182 pKa = 4.54 VIGQTFDD189 pKa = 2.91 SVTFVTASEE198 pKa = 4.57 YY199 pKa = 10.52 PVDD202 pKa = 3.95 RR203 pKa = 11.84 SSAYY207 pKa = 9.83 QCLTRR212 pKa = 11.84 HH213 pKa = 6.01 RR214 pKa = 11.84 KK215 pKa = 8.93 SLLILAPNASYY226 pKa = 11.34 SSAA229 pKa = 3.28
Molecular weight: 25.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.254
IPC2_protein 5.372
IPC_protein 5.334
Toseland 5.486
ProMoST 5.575
Dawson 5.461
Bjellqvist 5.525
Wikipedia 5.397
Rodwell 5.41
Grimsley 5.499
Solomon 5.461
Lehninger 5.436
Nozaki 5.652
DTASelect 5.817
Thurlkill 5.664
EMBOSS 5.614
Sillero 5.741
Patrickios 3.605
IPC_peptide 5.474
IPC2_peptide 5.741
IPC2.peptide.svr19 5.752
Protein with the highest isoelectric point:
>tr|A0A2P1JI50|A0A2P1JI50_9VIRU Triple gene block protein 1 OS=Chrysanthemum virus R OX=2116736 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.95 PLTPPPDD8 pKa = 3.28 HH9 pKa = 6.72 TKK11 pKa = 11.0 AFLAIAVGFSCALIISLFTRR31 pKa = 11.84 STLPQVGDD39 pKa = 3.8 QIHH42 pKa = 6.21 SLPHH46 pKa = 5.54 GGWYY50 pKa = 9.97 KK51 pKa = 11.07 DD52 pKa = 3.38 GTKK55 pKa = 10.0 QIYY58 pKa = 9.32 YY59 pKa = 10.48 GRR61 pKa = 11.84 PNKK64 pKa = 10.26 LNSVEE69 pKa = 4.12 KK70 pKa = 10.53 QGFLIGQPWAIVIILIALIILSSFRR95 pKa = 11.84 GPGRR99 pKa = 11.84 CMVCGQRR106 pKa = 11.84 HH107 pKa = 4.24
Molecular weight: 11.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.205
IPC2_protein 9.033
IPC_protein 9.063
Toseland 9.604
ProMoST 9.399
Dawson 9.867
Bjellqvist 9.663
Wikipedia 10.043
Rodwell 10.145
Grimsley 9.94
Solomon 9.955
Lehninger 9.926
Nozaki 9.823
DTASelect 9.589
Thurlkill 9.721
EMBOSS 10.028
Sillero 9.853
Patrickios 7.527
IPC_peptide 9.94
IPC2_peptide 8.712
IPC2.peptide.svr19 7.87
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2888
63
2075
481.3
54.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.752 ± 1.041
2.77 ± 0.472
4.363 ± 0.351
8.241 ± 1.033
4.605 ± 0.497
6.717 ± 0.402
2.528 ± 0.34
5.298 ± 0.676
6.717 ± 0.913
8.934 ± 0.503
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.355 ± 0.36
3.913 ± 0.373
4.12 ± 0.42
2.597 ± 0.379
6.44 ± 0.805
7.133 ± 0.157
5.436 ± 0.433
6.094 ± 0.623
1.108 ± 0.22
3.878 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here