Thermosynechococcus elongatus (strain BP-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Thermosynechococcus; Thermosynechococcus elongatus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0A420|PSAD_THEEB Photosystem I reaction center subunit II OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=psaD PE=1 SV=2
MM1 pKa = 7.26ATYY4 pKa = 10.58KK5 pKa = 9.48VTLVRR10 pKa = 11.84PDD12 pKa = 3.46GSEE15 pKa = 3.8TTIDD19 pKa = 3.72VPEE22 pKa = 5.09DD23 pKa = 3.66EE24 pKa = 5.4YY25 pKa = 11.14ILDD28 pKa = 3.69VAEE31 pKa = 4.2EE32 pKa = 4.07QGLDD36 pKa = 3.58LPFSCRR42 pKa = 11.84AGACSTCAGKK52 pKa = 10.41LLEE55 pKa = 4.65GEE57 pKa = 4.53VDD59 pKa = 3.63QSDD62 pKa = 4.17QSFLDD67 pKa = 3.85DD68 pKa = 4.08DD69 pKa = 4.27QIEE72 pKa = 4.3KK73 pKa = 10.92GFVLTCVAYY82 pKa = 9.42PRR84 pKa = 11.84SDD86 pKa = 3.59CKK88 pKa = 10.76ILTNQEE94 pKa = 3.75EE95 pKa = 4.15EE96 pKa = 4.77LYY98 pKa = 11.07

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8DL98|Q8DL98_THEEB Glutaminase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=tlr0598 PE=3 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTVRR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.12RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.4SGQNVIKK33 pKa = 10.46ARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.17GRR40 pKa = 11.84ARR42 pKa = 11.84LTVV45 pKa = 3.04

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2451

1

2452

770355

29

1706

314.2

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.77 ± 0.053

1.133 ± 0.018

4.456 ± 0.032

5.858 ± 0.052

3.524 ± 0.033

7.152 ± 0.052

2.203 ± 0.026

5.547 ± 0.034

3.098 ± 0.038

12.02 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.02

2.911 ± 0.031

5.776 ± 0.04

5.793 ± 0.048

6.604 ± 0.049

5.177 ± 0.03

5.503 ± 0.031

7.009 ± 0.037

1.69 ± 0.027

2.856 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski