Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes; unclassified Actinoplanes

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8SBS3|G8SBS3_ACTS5 Ornithine cyclodeaminase OS=Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110) OX=134676 GN=ACPL_4372 PE=4 SV=1
MM1 pKa = 7.54SDD3 pKa = 2.44HH4 pKa = 6.83WGGFDD9 pKa = 3.42HH10 pKa = 7.36HH11 pKa = 7.88DD12 pKa = 4.43DD13 pKa = 4.7GGDD16 pKa = 3.11HH17 pKa = 6.92HH18 pKa = 8.1LPDD21 pKa = 5.32FDD23 pKa = 6.04DD24 pKa = 4.15SHH26 pKa = 7.21DD27 pKa = 4.04LPAGHH32 pKa = 7.71DD33 pKa = 3.8LTDD36 pKa = 3.37MSDD39 pKa = 2.95LHH41 pKa = 6.9DD42 pKa = 3.85QHH44 pKa = 8.14DD45 pKa = 4.41HH46 pKa = 7.66DD47 pKa = 6.45DD48 pKa = 4.36PGHH51 pKa = 7.34DD52 pKa = 4.82DD53 pKa = 3.42AHH55 pKa = 7.8HH56 pKa = 7.81DD57 pKa = 4.23DD58 pKa = 4.25AQHH61 pKa = 7.31DD62 pKa = 3.76IAEE65 pKa = 4.64IPDD68 pKa = 3.61THH70 pKa = 7.16HH71 pKa = 7.54DD72 pKa = 3.97PVALTDD78 pKa = 4.39DD79 pKa = 4.86FPPHH83 pKa = 7.42LEE85 pKa = 4.0VGEE88 pKa = 4.22LPEE91 pKa = 4.66PVDD94 pKa = 3.62GFPWTDD100 pKa = 2.91AATLGHH106 pKa = 6.79AAAYY110 pKa = 7.73TPTTDD115 pKa = 4.01PVDD118 pKa = 3.76PHH120 pKa = 7.51DD121 pKa = 4.04LAAYY125 pKa = 9.82AGLDD129 pKa = 3.57VPEE132 pKa = 5.7GSDD135 pKa = 2.78PWTALTASDD144 pKa = 4.85DD145 pKa = 4.02PAISALARR153 pKa = 11.84WWTPDD158 pKa = 3.17EE159 pKa = 4.11QQ160 pKa = 4.59

Molecular weight: 17.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8S7L1|G8S7L1_ACTS5 Putative hydrolase OS=Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110) OX=134676 GN=ACPL_7893 PE=4 SV=1
MM1 pKa = 6.91VAIAGRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84QAVMAIFGRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84QAVMAIFGQRR30 pKa = 11.84RR31 pKa = 11.84QVVMAIAGRR40 pKa = 11.84RR41 pKa = 11.84LPPVMVIAGRR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 4.29QAVMAMVSAPAATSTPAHH71 pKa = 6.04NRR73 pKa = 11.84STLNQARR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84MFTPRR87 pKa = 11.84NSS89 pKa = 3.1

Molecular weight: 9.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8249

0

8249

2710578

30

6598

328.6

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.323 ± 0.049

0.744 ± 0.007

6.054 ± 0.024

4.775 ± 0.025

2.718 ± 0.016

9.335 ± 0.037

2.198 ± 0.015

3.587 ± 0.018

1.775 ± 0.02

10.34 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.692 ± 0.011

1.867 ± 0.017

6.174 ± 0.031

2.831 ± 0.017

8.144 ± 0.032

4.911 ± 0.025

6.417 ± 0.034

8.511 ± 0.027

1.569 ± 0.011

2.035 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski