Amino acid dipepetide frequency for Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	25.022AlaAla: 25.022 ± 0.18	1.047AlaCys: 1.047 ± 0.022	9.118AlaAsp: 9.118 ± 0.079	8.207AlaGlu: 8.207 ± 0.082	3.442AlaPhe: 3.442 ± 0.044	15.736AlaGly: 15.736 ± 0.111	2.529AlaHis: 2.529 ± 0.034	4.474AlaIle: 4.474 ± 0.042	2.598AlaLys: 2.598 ± 0.05	13.914AlaLeu: 13.914 ± 0.099	2.582AlaMet: 2.582 ± 0.036	2.219AlaAsn: 2.219 ± 0.032	6.932AlaPro: 6.932 ± 0.07	3.783AlaGln: 3.783 ± 0.041	10.01AlaArg: 10.01 ± 0.075	5.688AlaSer: 5.688 ± 0.064	8.507AlaThr: 8.507 ± 0.066	12.709AlaVal: 12.709 ± 0.079	1.936AlaTrp: 1.936 ± 0.029	2.781AlaTyr: 2.781 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	0.978CysAla: 0.978 ± 0.025	0.087CysCys: 0.087 ± 0.006	0.467CysAsp: 0.467 ± 0.015	0.303CysGlu: 0.303 ± 0.01	0.189CysPhe: 0.189 ± 0.009	0.881CysGly: 0.881 ± 0.017	0.205CysHis: 0.205 ± 0.008	0.166CysIle: 0.166 ± 0.007	0.091CysLys: 0.091 ± 0.006	0.716CysLeu: 0.716 ± 0.016	0.113CysMet: 0.113 ± 0.007	0.145CysAsn: 0.145 ± 0.008	0.475CysPro: 0.475 ± 0.015	0.184CysGln: 0.184 ± 0.008	0.646CysArg: 0.646 ± 0.017	0.438CysSer: 0.438 ± 0.014	0.488CysThr: 0.488 ± 0.016	0.557CysVal: 0.557 ± 0.015	0.149CysTrp: 0.149 ± 0.01	0.16CysTyr: 0.16 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.534AspAla: 7.534 ± 0.058	0.415AspCys: 0.415 ± 0.013	3.888AspAsp: 3.888 ± 0.044	3.611AspGlu: 3.611 ± 0.043	1.61AspPhe: 1.61 ± 0.024	6.254AspGly: 6.254 ± 0.062	1.388AspHis: 1.388 ± 0.025	1.756AspIle: 1.756 ± 0.028	0.971AspLys: 0.971 ± 0.022	6.803AspLeu: 6.803 ± 0.058	0.785AspMet: 0.785 ± 0.018	1.078AspAsn: 1.078 ± 0.022	4.876AspPro: 4.876 ± 0.047	1.754AspGln: 1.754 ± 0.032	5.541AspArg: 5.541 ± 0.06	2.337AspSer: 2.337 ± 0.033	3.109AspThr: 3.109 ± 0.036	4.687AspVal: 4.687 ± 0.039	0.991AspTrp: 0.991 ± 0.021	1.151AspTyr: 1.151 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	6.203GluAla: 6.203 ± 0.065	0.3GluCys: 0.3 ± 0.011	2.039GluAsp: 2.039 ± 0.033	2.189GluGlu: 2.189 ± 0.037	1.383GluPhe: 1.383 ± 0.023	2.803GluGly: 2.803 ± 0.036	1.372GluHis: 1.372 ± 0.026	2.301GluIle: 2.301 ± 0.035	1.061GluLys: 1.061 ± 0.02	5.902GluLeu: 5.902 ± 0.06	0.889GluMet: 0.889 ± 0.02	0.881GluAsn: 0.881 ± 0.02	3.257GluPro: 3.257 ± 0.044	2.172GluGln: 2.172 ± 0.034	4.451GluArg: 4.451 ± 0.055	2.208GluSer: 2.208 ± 0.034	2.596GluThr: 2.596 ± 0.035	4.152GluVal: 4.152 ± 0.05	0.648GluTrp: 0.648 ± 0.015	0.94GluTyr: 0.94 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.918PheAla: 3.918 ± 0.048	0.253PheCys: 0.253 ± 0.01	1.981PheAsp: 1.981 ± 0.03	1.198PheGlu: 1.198 ± 0.021	0.834PhePhe: 0.834 ± 0.021	2.997PheGly: 2.997 ± 0.032	0.6PheHis: 0.6 ± 0.018	0.709PheIle: 0.709 ± 0.018	0.439PheLys: 0.439 ± 0.015	2.572PheLeu: 2.572 ± 0.037	0.342PheMet: 0.342 ± 0.012	0.552PheAsn: 0.552 ± 0.016	1.445PhePro: 1.445 ± 0.024	0.698PheGln: 0.698 ± 0.017	1.956PheArg: 1.956 ± 0.027	1.358PheSer: 1.358 ± 0.025	2.051PheThr: 2.051 ± 0.032	2.21PheVal: 2.21 ± 0.03	0.455PheTrp: 0.455 ± 0.013	0.611PheTyr: 0.611 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.126GlyAla: 11.126 ± 0.086	0.806GlyCys: 0.806 ± 0.019	5.59GlyAsp: 5.59 ± 0.05	4.335GlyGlu: 4.335 ± 0.049	2.908GlyPhe: 2.908 ± 0.034	8.557GlyGly: 8.557 ± 0.083	2.304GlyHis: 2.304 ± 0.033	3.416GlyIle: 3.416 ± 0.04	1.999GlyLys: 1.999 ± 0.03	9.446GlyLeu: 9.446 ± 0.078	1.872GlyMet: 1.872 ± 0.029	1.905GlyAsn: 1.905 ± 0.041	5.145GlyPro: 5.145 ± 0.056	2.73GlyGln: 2.73 ± 0.035	7.932GlyArg: 7.932 ± 0.053	5.136GlySer: 5.136 ± 0.064	6.213GlyThr: 6.213 ± 0.061	7.668GlyVal: 7.668 ± 0.058	1.894GlyTrp: 1.894 ± 0.028	2.372GlyTyr: 2.372 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.588HisAla: 2.588 ± 0.032	0.184HisCys: 0.184 ± 0.008	1.449HisAsp: 1.449 ± 0.028	1.08HisGlu: 1.08 ± 0.019	0.587HisPhe: 0.587 ± 0.014	2.173HisGly: 2.173 ± 0.031	0.701HisHis: 0.701 ± 0.017	0.594HisIle: 0.594 ± 0.015	0.262HisLys: 0.262 ± 0.009	2.515HisLeu: 2.515 ± 0.038	0.294HisMet: 0.294 ± 0.01	0.383HisAsn: 0.383 ± 0.014	1.818HisPro: 1.818 ± 0.031	0.671HisGln: 0.671 ± 0.015	2.228HisArg: 2.228 ± 0.035	0.91HisSer: 0.91 ± 0.019	1.19HisThr: 1.19 ± 0.022	1.565HisVal: 1.565 ± 0.027	0.334HisTrp: 0.334 ± 0.011	0.455HisTyr: 0.455 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	5.491IleAla: 5.491 ± 0.055	0.324IleCys: 0.324 ± 0.012	2.549IleAsp: 2.549 ± 0.034	1.973IleGlu: 1.973 ± 0.03	0.897IlePhe: 0.897 ± 0.02	3.903IleGly: 3.903 ± 0.039	0.602IleHis: 0.602 ± 0.016	1.1IleIle: 1.1 ± 0.023	0.708IleLys: 0.708 ± 0.017	2.851IleLeu: 2.851 ± 0.036	0.491IleMet: 0.491 ± 0.014	0.819IleAsn: 0.819 ± 0.018	1.896IlePro: 1.896 ± 0.028	0.778IleGln: 0.778 ± 0.018	2.743IleArg: 2.743 ± 0.034	1.925IleSer: 1.925 ± 0.027	2.672IleThr: 2.672 ± 0.034	3.084IleVal: 3.084 ± 0.041	0.449IleTrp: 0.449 ± 0.015	0.617IleTyr: 0.617 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.394LysAla: 2.394 ± 0.05	0.107LysCys: 0.107 ± 0.007	0.943LysAsp: 0.943 ± 0.023	0.715LysGlu: 0.715 ± 0.021	0.426LysPhe: 0.426 ± 0.015	1.275LysGly: 1.275 ± 0.024	0.352LysHis: 0.352 ± 0.011	0.853LysIle: 0.853 ± 0.023	0.563LysLys: 0.563 ± 0.02	1.821LysLeu: 1.821 ± 0.031	0.334LysMet: 0.334 ± 0.011	0.454LysAsn: 0.454 ± 0.015	1.265LysPro: 1.265 ± 0.028	0.632LysGln: 0.632 ± 0.017	1.269LysArg: 1.269 ± 0.023	0.992LysSer: 0.992 ± 0.021	1.121LysThr: 1.121 ± 0.026	1.613LysVal: 1.613 ± 0.03	0.231LysTrp: 0.231 ± 0.009	0.387LysTyr: 0.387 ± 0.012	0.0LysXaa: 0.0 ± 0.0
Leu	15.791LeuAla: 15.791 ± 0.116	0.74LeuCys: 0.74 ± 0.018	6.552LeuAsp: 6.552 ± 0.054	3.747LeuGlu: 3.747 ± 0.043	2.629LeuPhe: 2.629 ± 0.035	9.001LeuGly: 9.001 ± 0.061	2.323LeuHis: 2.323 ± 0.031	4.195LeuIle: 4.195 ± 0.041	1.575LeuLys: 1.575 ± 0.034	11.49LeuLeu: 11.49 ± 0.097	1.44LeuMet: 1.44 ± 0.024	1.781LeuAsn: 1.781 ± 0.028	6.523LeuPro: 6.523 ± 0.061	2.352LeuGln: 2.352 ± 0.032	8.826LeuArg: 8.826 ± 0.082	5.397LeuSer: 5.397 ± 0.048	7.543LeuThr: 7.543 ± 0.063	8.512LeuVal: 8.512 ± 0.065	1.276LeuTrp: 1.276 ± 0.027	1.71LeuTyr: 1.71 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.17MetAla: 2.17 ± 0.03	0.113MetCys: 0.113 ± 0.007	0.777MetAsp: 0.777 ± 0.016	0.545MetGlu: 0.545 ± 0.016	0.468MetPhe: 0.468 ± 0.013	1.095MetGly: 1.095 ± 0.022	0.321MetHis: 0.321 ± 0.01	0.836MetIle: 0.836 ± 0.018	0.344MetLys: 0.344 ± 0.012	1.899MetLeu: 1.899 ± 0.028	0.292MetMet: 0.292 ± 0.01	0.404MetAsn: 0.404 ± 0.013	1.166MetPro: 1.166 ± 0.022	0.439MetGln: 0.439 ± 0.013	1.443MetArg: 1.443 ± 0.024	1.218MetSer: 1.218 ± 0.021	1.603MetThr: 1.603 ± 0.026	1.3MetVal: 1.3 ± 0.024	0.201MetTrp: 0.201 ± 0.008	0.289MetTyr: 0.289 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.379AsnAla: 2.379 ± 0.036	0.172AsnCys: 0.172 ± 0.008	1.04AsnAsp: 1.04 ± 0.026	0.754AsnGlu: 0.754 ± 0.019	0.473AsnPhe: 0.473 ± 0.013	2.071AsnGly: 2.071 ± 0.042	0.381AsnHis: 0.381 ± 0.012	0.602AsnIle: 0.602 ± 0.017	0.341AsnLys: 0.341 ± 0.014	1.861AsnLeu: 1.861 ± 0.027	0.288AsnMet: 0.288 ± 0.012	0.512AsnAsn: 0.512 ± 0.02	1.413AsnPro: 1.413 ± 0.026	0.592AsnGln: 0.592 ± 0.017	1.555AsnArg: 1.555 ± 0.027	0.909AsnSer: 0.909 ± 0.02	1.151AsnThr: 1.151 ± 0.024	1.433AsnVal: 1.433 ± 0.029	0.317AsnTrp: 0.317 ± 0.012	0.422AsnTyr: 0.422 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	10.387ProAla: 10.387 ± 0.101	0.286ProCys: 0.286 ± 0.01	4.584ProAsp: 4.584 ± 0.045	3.553ProGlu: 3.553 ± 0.047	1.471ProPhe: 1.471 ± 0.028	6.81ProGly: 6.81 ± 0.064	1.271ProHis: 1.271 ± 0.022	1.927ProIle: 1.927 ± 0.028	0.935ProLys: 0.935 ± 0.02	5.116ProLeu: 5.116 ± 0.047	0.998ProMet: 0.998 ± 0.02	0.961ProAsn: 0.961 ± 0.02	3.779ProPro: 3.779 ± 0.061	1.645ProGln: 1.645 ± 0.03	3.869ProArg: 3.869 ± 0.039	3.077ProSer: 3.077 ± 0.044	3.237ProThr: 3.237 ± 0.042	5.731ProVal: 5.731 ± 0.054	0.926ProTrp: 0.926 ± 0.018	1.173ProTyr: 1.173 ± 0.021	0.0ProXaa: 0.0 ± 0.0
Gln	4.063GlnAla: 4.063 ± 0.045	0.158GlnCys: 0.158 ± 0.008	1.229GlnAsp: 1.229 ± 0.026	1.133GlnGlu: 1.133 ± 0.019	0.761GlnPhe: 0.761 ± 0.019	1.867GlnGly: 1.867 ± 0.03	0.699GlnHis: 0.699 ± 0.016	1.246GlnIle: 1.246 ± 0.02	0.571GlnLys: 0.571 ± 0.016	3.104GlnLeu: 3.104 ± 0.041	0.512GlnMet: 0.512 ± 0.014	0.579GlnAsn: 0.579 ± 0.016	1.925GlnPro: 1.925 ± 0.026	1.308GlnGln: 1.308 ± 0.025	2.678GlnArg: 2.678 ± 0.033	1.271GlnSer: 1.271 ± 0.022	1.528GlnThr: 1.528 ± 0.025	2.631GlnVal: 2.631 ± 0.031	0.492GlnTrp: 0.492 ± 0.014	0.555GlnTyr: 0.555 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	10.037ArgAla: 10.037 ± 0.083	0.597ArgCys: 0.597 ± 0.013	4.701ArgAsp: 4.701 ± 0.051	4.019ArgGlu: 4.019 ± 0.049	2.451ArgPhe: 2.451 ± 0.032	5.566ArgGly: 5.566 ± 0.059	2.23ArgHis: 2.23 ± 0.029	3.468ArgIle: 3.468 ± 0.038	1.337ArgLys: 1.337 ± 0.026	9.258ArgLeu: 9.258 ± 0.069	1.89ArgMet: 1.89 ± 0.027	1.459ArgAsn: 1.459 ± 0.027	5.075ArgPro: 5.075 ± 0.047	2.571ArgGln: 2.571 ± 0.037	8.251ArgArg: 8.251 ± 0.089	3.968ArgSer: 3.968 ± 0.039	4.909ArgThr: 4.909 ± 0.045	6.133ArgVal: 6.133 ± 0.046	1.599ArgTrp: 1.599 ± 0.024	1.918ArgTyr: 1.918 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	6.859SerAla: 6.859 ± 0.066	0.374SerCys: 0.374 ± 0.013	2.584SerAsp: 2.584 ± 0.034	1.89SerGlu: 1.89 ± 0.031	1.489SerPhe: 1.489 ± 0.025	5.801SerGly: 5.801 ± 0.065	0.904SerHis: 0.904 ± 0.021	1.748SerIle: 1.748 ± 0.032	0.814SerLys: 0.814 ± 0.019	4.511SerLeu: 4.511 ± 0.042	1.057SerMet: 1.057 ± 0.021	0.877SerAsn: 0.877 ± 0.022	3.216SerPro: 3.216 ± 0.038	1.181SerGln: 1.181 ± 0.024	3.76SerArg: 3.76 ± 0.038	2.765SerSer: 2.765 ± 0.047	3.152SerThr: 3.152 ± 0.044	4.042SerVal: 4.042 ± 0.039	0.946SerTrp: 0.946 ± 0.019	1.143SerTyr: 1.143 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.522ThrAla: 9.522 ± 0.077	0.467ThrCys: 0.467 ± 0.016	3.714ThrAsp: 3.714 ± 0.043	2.829ThrGlu: 2.829 ± 0.038	1.759ThrPhe: 1.759 ± 0.026	7.383ThrGly: 7.383 ± 0.067	1.085ThrHis: 1.085 ± 0.021	2.264ThrIle: 2.264 ± 0.034	1.021ThrLys: 1.021 ± 0.028	6.015ThrLeu: 6.015 ± 0.053	1.046ThrMet: 1.046 ± 0.023	1.086ThrAsn: 1.086 ± 0.026	4.222ThrPro: 4.222 ± 0.039	1.286ThrGln: 1.286 ± 0.025	4.091ThrArg: 4.091 ± 0.043	3.166ThrSer: 3.166 ± 0.045	4.273ThrThr: 4.273 ± 0.065	6.595ThrVal: 6.595 ± 0.06	0.988ThrTrp: 0.988 ± 0.022	1.357ThrTyr: 1.357 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	12.216ValAla: 12.216 ± 0.078	0.68ValCys: 0.68 ± 0.014	5.138ValAsp: 5.138 ± 0.039	4.126ValGlu: 4.126 ± 0.048	2.267ValPhe: 2.267 ± 0.029	6.266ValGly: 6.266 ± 0.053	1.799ValHis: 1.799 ± 0.027	3.22ValIle: 3.22 ± 0.036	1.54ValLys: 1.54 ± 0.031	9.442ValLeu: 9.442 ± 0.084	1.207ValMet: 1.207 ± 0.022	1.747ValAsn: 1.747 ± 0.028	5.272ValPro: 5.272 ± 0.049	2.124ValGln: 2.124 ± 0.028	6.749ValArg: 6.749 ± 0.049	4.389ValSer: 4.389 ± 0.045	6.472ValThr: 6.472 ± 0.069	7.854ValVal: 7.854 ± 0.067	1.112ValTrp: 1.112 ± 0.022	1.486ValTyr: 1.486 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.797TrpAla: 1.797 ± 0.03	0.158TrpCys: 0.158 ± 0.009	0.808TrpAsp: 0.808 ± 0.017	0.609TrpGlu: 0.609 ± 0.015	0.501TrpPhe: 0.501 ± 0.014	0.979TrpGly: 0.979 ± 0.022	0.442TrpHis: 0.442 ± 0.013	0.57TrpIle: 0.57 ± 0.014	0.287TrpLys: 0.287 ± 0.012	1.856TrpLeu: 1.856 ± 0.034	0.274TrpMet: 0.274 ± 0.01	0.418TrpAsn: 0.418 ± 0.017	0.889TrpPro: 0.889 ± 0.021	0.688TrpGln: 0.688 ± 0.015	1.468TrpArg: 1.468 ± 0.025	1.032TrpSer: 1.032 ± 0.02	1.108TrpThr: 1.108 ± 0.022	1.052TrpVal: 1.052 ± 0.021	0.394TrpTrp: 0.394 ± 0.012	0.359TrpTyr: 0.359 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.758TyrAla: 2.758 ± 0.032	0.175TyrCys: 0.175 ± 0.008	1.389TyrAsp: 1.389 ± 0.033	0.933TyrGlu: 0.933 ± 0.023	0.635TyrPhe: 0.635 ± 0.016	1.936TyrGly: 1.936 ± 0.032	0.473TyrHis: 0.473 ± 0.014	0.427TyrIle: 0.427 ± 0.013	0.291TyrLys: 0.291 ± 0.012	2.31TyrLeu: 2.31 ± 0.025	0.215TyrMet: 0.215 ± 0.009	0.404TyrAsn: 0.404 ± 0.013	1.147TyrPro: 1.147 ± 0.024	0.721TyrGln: 0.721 ± 0.019	1.969TyrArg: 1.969 ± 0.03	0.93TyrSer: 0.93 ± 0.018	1.246TyrThr: 1.246 ± 0.026	1.577TyrVal: 1.577 ± 0.025	0.351TyrTrp: 0.351 ± 0.01	0.467TyrTyr: 0.467 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8249 proteins (2710578 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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