Bacterioplanes sanyensis
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3784 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A222FHF0|A0A222FHF0_9GAMM N-acetyltransferase domain-containing protein OS=Bacterioplanes sanyensis OX=1249553 GN=CHH28_04085 PE=4 SV=1
MM1 pKa = 7.61 ASSAQAQFIINEE13 pKa = 4.21 LDD15 pKa = 3.27 ADD17 pKa = 3.93 QAGTDD22 pKa = 3.22 SAEE25 pKa = 4.33 FIEE28 pKa = 6.05 LYY30 pKa = 10.76 DD31 pKa = 4.39 GGLGNQPLDD40 pKa = 3.91 GYY42 pKa = 11.15 SLVLYY47 pKa = 9.85 NGSGSKK53 pKa = 10.15 SYY55 pKa = 11.47 NSVDD59 pKa = 3.27 LSGYY63 pKa = 7.4 STNADD68 pKa = 3.11 GFFVVCGDD76 pKa = 3.62 TAAVANCMLDD86 pKa = 2.57 IGTNTNLIQNGADD99 pKa = 3.17 AAALYY104 pKa = 9.72 ARR106 pKa = 11.84 PASDD110 pKa = 4.18 FPSNTPVDD118 pKa = 3.91 VNGVVDD124 pKa = 4.15 ALVYY128 pKa = 8.56 DD129 pKa = 4.51 TNDD132 pKa = 3.1 SDD134 pKa = 4.05 ATALLALLNAGQPQVNEE151 pKa = 3.95 AAGGDD156 pKa = 3.72 KK157 pKa = 10.4 DD158 pKa = 3.54 AHH160 pKa = 6.29 SNQRR164 pKa = 11.84 CGDD167 pKa = 4.32 GIARR171 pKa = 11.84 NTDD174 pKa = 3.38 GYY176 pKa = 10.17 RR177 pKa = 11.84 QQAPTPGAANQCDD190 pKa = 4.89 GIDD193 pKa = 3.96 PDD195 pKa = 4.78 PGIGACGDD203 pKa = 3.73 VSHH206 pKa = 7.33 SDD208 pKa = 3.51 YY209 pKa = 11.42 QLISSIQGRR218 pKa = 11.84 IEE220 pKa = 4.82 DD221 pKa = 4.18 SANDD225 pKa = 3.71 ASPLNGQTVVVEE237 pKa = 4.26 AVVTTDD243 pKa = 3.46 LQGGQLANGDD253 pKa = 3.52 NSFQYY258 pKa = 10.11 SGYY261 pKa = 9.61 WLQQLEE267 pKa = 4.38 SEE269 pKa = 4.65 YY270 pKa = 11.42 DD271 pKa = 3.22 NDD273 pKa = 3.78 AQTSEE278 pKa = 4.71 GIFVYY283 pKa = 10.05 DD284 pKa = 3.89 FRR286 pKa = 11.84 SEE288 pKa = 4.08 VSVGDD293 pKa = 3.68 RR294 pKa = 11.84 VRR296 pKa = 11.84 LQAQVSEE303 pKa = 5.14 FNQSTQLKK311 pKa = 8.75 NVADD315 pKa = 4.29 LVVCASGQSLPAPVALQLPVSDD337 pKa = 3.43 KK338 pKa = 10.13 TAWEE342 pKa = 4.09 AVEE345 pKa = 4.54 GMLVSSQQNLVVSDD359 pKa = 4.37 LFGTGYY365 pKa = 11.26 GFGNYY370 pKa = 8.93 GQFVVSSRR378 pKa = 11.84 LHH380 pKa = 4.92 FQPTEE385 pKa = 3.91 VEE387 pKa = 4.45 LPGSAAAQALAAARR401 pKa = 11.84 PLDD404 pKa = 4.14 ALLIDD409 pKa = 4.74 DD410 pKa = 5.42 GVAKK414 pKa = 10.2 SYY416 pKa = 10.3 PAFIPFPDD424 pKa = 3.4 EE425 pKa = 4.35 SGFSASNPMRR435 pKa = 11.84 IGYY438 pKa = 7.29 QVPSVSGVMNEE449 pKa = 3.6 FRR451 pKa = 11.84 DD452 pKa = 4.05 NYY454 pKa = 9.76 TVIPNNIVIDD464 pKa = 3.93 PVAARR469 pKa = 11.84 TAAPQVALDD478 pKa = 3.75 ADD480 pKa = 4.22 LVVVGMNVLNYY491 pKa = 10.14 FNGDD495 pKa = 3.22 GQGGGFPTSRR505 pKa = 11.84 GAPSAAAFEE514 pKa = 4.26 MQTAKK519 pKa = 10.2 IVAALQAMDD528 pKa = 4.03 ADD530 pKa = 3.68 IVGLMEE536 pKa = 5.2 IEE538 pKa = 3.83 NDD540 pKa = 4.14 GYY542 pKa = 11.7 GSDD545 pKa = 3.55 SAIASLVNALNAVQAAGDD563 pKa = 3.82 EE564 pKa = 4.31 YY565 pKa = 10.83 TYY567 pKa = 11.44 VNPGRR572 pKa = 11.84 NQVGTDD578 pKa = 4.18 AIAVGLLYY586 pKa = 10.58 RR587 pKa = 11.84 PAKK590 pKa = 10.28 VSLEE594 pKa = 4.06 GASVILDD601 pKa = 3.66 SSNSPLDD608 pKa = 3.53 DD609 pKa = 3.18 TGAPLFIDD617 pKa = 3.52 NKK619 pKa = 10.15 NRR621 pKa = 11.84 PSLIQSFRR629 pKa = 11.84 YY630 pKa = 9.5 NDD632 pKa = 3.59 TVFTLSVNHH641 pKa = 6.54 LKK643 pKa = 10.89 SKK645 pKa = 10.51 GSPCGEE651 pKa = 4.1 PNEE654 pKa = 4.54 GEE656 pKa = 4.17 NGVGSCNLTRR666 pKa = 11.84 TTAAQALVQFLATNPTGVDD685 pKa = 3.0 SDD687 pKa = 3.7 ATLILGDD694 pKa = 4.17 LNAYY698 pKa = 8.29 SQEE701 pKa = 4.24 DD702 pKa = 3.95 PMQVFYY708 pKa = 11.12 QGGFTNLKK716 pKa = 8.26 YY717 pKa = 9.15 TDD719 pKa = 4.2 KK720 pKa = 11.11 VSEE723 pKa = 4.09 QQPFSYY729 pKa = 10.4 SFSGFLGSLDD739 pKa = 3.66 HH740 pKa = 7.12 ALASPSLLNHH750 pKa = 5.64 VVSVDD755 pKa = 2.75 AWHH758 pKa = 6.76 INSVEE763 pKa = 4.04 APLMDD768 pKa = 4.34 YY769 pKa = 9.71 LTEE772 pKa = 4.85 ANGQDD777 pKa = 3.78 FDD779 pKa = 6.38 SIDD782 pKa = 3.38 NYY784 pKa = 11.14 AAADD788 pKa = 3.95 AYY790 pKa = 10.59 RR791 pKa = 11.84 SSDD794 pKa = 3.13 HH795 pKa = 7.23 DD796 pKa = 4.79 PIVLGLSFAAANQAPQQSDD815 pKa = 4.21 DD816 pKa = 3.65 IADD819 pKa = 3.79 VALEE823 pKa = 4.06 QADD826 pKa = 3.88 TLYY829 pKa = 11.12 SIDD832 pKa = 3.59 LSQYY836 pKa = 10.65 FIDD839 pKa = 4.81 ADD841 pKa = 3.94 GDD843 pKa = 4.0 ALSYY847 pKa = 10.9 EE848 pKa = 4.74 LDD850 pKa = 3.69 GQQAGITLLATGEE863 pKa = 4.22 LQVQLTAEE871 pKa = 4.24 QLDD874 pKa = 4.2 SLPLTLAFAVTDD886 pKa = 4.35 GQASIAATITLLDD899 pKa = 3.59 QRR901 pKa = 11.84 PAQSPWQRR909 pKa = 11.84 WWQWLQDD916 pKa = 3.18 WWKK919 pKa = 10.66 SLWDD923 pKa = 3.52
Molecular weight: 97.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.706
IPC_protein 3.77
Toseland 3.528
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.164
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.884
Patrickios 0.858
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|A0A222FFY9|A0A222FFY9_9GAMM Trk system potassium uptake protein OS=Bacterioplanes sanyensis OX=1249553 GN=CHH28_04520 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 HH40 pKa = 4.64 VLSAA44 pKa = 3.81
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3784
0
3784
1254103
37
6764
331.4
36.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.042 ± 0.04
1.009 ± 0.017
5.999 ± 0.034
5.74 ± 0.038
3.55 ± 0.023
7.002 ± 0.04
2.541 ± 0.022
5.037 ± 0.028
3.361 ± 0.032
10.993 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.497 ± 0.022
3.434 ± 0.029
4.27 ± 0.025
6.151 ± 0.046
5.75 ± 0.036
6.419 ± 0.04
4.981 ± 0.031
6.874 ± 0.038
1.564 ± 0.02
2.785 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here