Salinibacterium xinjiangense
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2810 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2C8YSW6|A0A2C8YSW6_9MICO Fur family transcriptional regulator ferric uptake regulator OS=Salinibacterium xinjiangense OX=386302 GN=SAMN06296378_0608 PE=3 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 ITRR5 pKa = 11.84 TRR7 pKa = 11.84 AIIVTGLVAGSLALTACSSPAAEE30 pKa = 4.6 EE31 pKa = 4.04 PASPSAAGAIDD42 pKa = 4.47 LAAAGCPADD51 pKa = 5.46 IKK53 pKa = 10.94 IQTDD57 pKa = 3.37 WNPEE61 pKa = 3.83 AEE63 pKa = 4.3 HH64 pKa = 5.63 GHH66 pKa = 6.89 LYY68 pKa = 10.94 GLLGSDD74 pKa = 3.59 YY75 pKa = 11.26 TVDD78 pKa = 3.57 TTNKK82 pKa = 8.59 TVTGPLVVGGEE93 pKa = 4.09 PTGVNVTILAGGPAVGFSQPNAQLYY118 pKa = 10.32 SDD120 pKa = 5.2 DD121 pKa = 5.03 SIFMAYY127 pKa = 10.13 VGTDD131 pKa = 3.07 EE132 pKa = 6.01 AIAHH136 pKa = 5.86 SVDD139 pKa = 3.77 LPTVGVFQPLEE150 pKa = 4.42 KK151 pKa = 10.35 DD152 pKa = 3.25 PQMIMWDD159 pKa = 3.49 PATYY163 pKa = 10.24 PDD165 pKa = 3.93 VEE167 pKa = 4.84 SIADD171 pKa = 3.93 LGKK174 pKa = 8.01 TDD176 pKa = 3.1 APIRR180 pKa = 11.84 VFPGGVYY187 pKa = 9.07 IDD189 pKa = 4.05 YY190 pKa = 10.83 FIGTGVLSADD200 pKa = 4.36 QIDD203 pKa = 3.7 ATYY206 pKa = 10.43 DD207 pKa = 3.14 GSPAVFVSEE216 pKa = 4.33 GGKK219 pKa = 7.75 TAQQGFASAEE229 pKa = 3.73 PYY231 pKa = 9.81 IYY233 pKa = 10.56 KK234 pKa = 10.48 NEE236 pKa = 3.83 VAEE239 pKa = 4.05 WGKK242 pKa = 10.11 DD243 pKa = 3.28 VKK245 pKa = 11.08 YY246 pKa = 10.84 QLVNDD251 pKa = 4.21 AGFPKK256 pKa = 9.1 YY257 pKa = 10.72 AAMVSVRR264 pKa = 11.84 PDD266 pKa = 3.27 DD267 pKa = 3.49 VTAYY271 pKa = 10.44 ADD273 pKa = 3.87 CLTALVPVMQQAEE286 pKa = 3.79 IDD288 pKa = 4.26 YY289 pKa = 11.16 YY290 pKa = 11.47 DD291 pKa = 4.03 GTTLDD296 pKa = 3.63 ATNALILDD304 pKa = 4.49 LVDD307 pKa = 4.02 QYY309 pKa = 11.75 DD310 pKa = 3.85 TGWVYY315 pKa = 10.99 SQGVADD321 pKa = 4.24 YY322 pKa = 10.97 SVKK325 pKa = 9.49 TQLADD330 pKa = 4.18 EE331 pKa = 4.59 IVSNGPDD338 pKa = 3.05 ATLGNFDD345 pKa = 4.22 DD346 pKa = 4.61 ARR348 pKa = 11.84 VAEE351 pKa = 4.6 LFDD354 pKa = 3.46 IVSPIFNKK362 pKa = 9.92 QDD364 pKa = 2.76 ITIADD369 pKa = 3.87 GLAPTDD375 pKa = 3.98 LYY377 pKa = 11.22 TNEE380 pKa = 4.24 FVDD383 pKa = 3.69 MSLGLKK389 pKa = 9.76
Molecular weight: 41.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.923
Patrickios 0.858
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A2C9A2H8|A0A2C9A2H8_9MICO Peptidyl-prolyl cis-trans isomerase B (Cyclophilin B) OS=Salinibacterium xinjiangense OX=386302 GN=SAMN06296378_2753 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2810
0
2810
898122
29
2018
319.6
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.546 ± 0.06
0.509 ± 0.011
5.997 ± 0.044
5.292 ± 0.048
3.383 ± 0.028
8.736 ± 0.042
1.895 ± 0.02
5.305 ± 0.035
2.349 ± 0.034
10.063 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.923 ± 0.019
2.443 ± 0.026
5.132 ± 0.03
2.881 ± 0.023
6.591 ± 0.045
6.457 ± 0.037
6.236 ± 0.043
8.811 ± 0.046
1.431 ± 0.02
2.018 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here