Terracoccus luteus
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3555 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A495Y1Q0|A0A495Y1Q0_9MICO L-lactate permease OS=Terracoccus luteus OX=53356 GN=DFJ68_2320 PE=3 SV=1
MM1 pKa = 7.44 SLFITCPVEE10 pKa = 4.1 SVEE13 pKa = 4.5 RR14 pKa = 11.84 ATAFYY19 pKa = 10.51 TDD21 pKa = 5.27 LGWTLNAEE29 pKa = 4.27 MSDD32 pKa = 3.71 HH33 pKa = 6.28 NVSCFAIAPEE43 pKa = 4.12 QYY45 pKa = 11.19 VMLGSRR51 pKa = 11.84 EE52 pKa = 4.01 MYY54 pKa = 10.34 ASVGGVEE61 pKa = 4.18 QLVGDD66 pKa = 4.59 ADD68 pKa = 4.17 TPSKK72 pKa = 10.21 VTVSFDD78 pKa = 3.2 LGSRR82 pKa = 11.84 EE83 pKa = 3.99 AVDD86 pKa = 3.89 ALVEE90 pKa = 4.07 RR91 pKa = 11.84 AGAAGGRR98 pKa = 11.84 VGDD101 pKa = 3.62 TDD103 pKa = 4.55 EE104 pKa = 4.55 YY105 pKa = 10.25 PFMYY109 pKa = 9.89 QRR111 pKa = 11.84 QFDD114 pKa = 4.24 DD115 pKa = 3.81 LDD117 pKa = 4.38 GYY119 pKa = 10.87 HH120 pKa = 6.72 YY121 pKa = 11.3 SPFWMKK127 pKa = 10.69 PDD129 pKa = 4.09 GDD131 pKa = 3.87 PTAA134 pKa = 4.95
Molecular weight: 14.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 4.05
IPC_protein 3.999
Toseland 3.795
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.923
Rodwell 3.834
Grimsley 3.719
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.329
Thurlkill 3.846
EMBOSS 3.923
Sillero 4.126
Patrickios 1.939
IPC_peptide 3.973
IPC2_peptide 4.101
IPC2.peptide.svr19 4.005
Protein with the highest isoelectric point:
>tr|A0A495XWW1|A0A495XWW1_9MICO Anti-sigma factor antagonist OS=Terracoccus luteus OX=53356 GN=DFJ68_2526 PE=3 SV=1
MM1 pKa = 7.18 VPASPVPRR9 pKa = 11.84 RR10 pKa = 11.84 LLVRR14 pKa = 11.84 RR15 pKa = 11.84 DD16 pKa = 3.33 RR17 pKa = 11.84 VHH19 pKa = 6.62 RR20 pKa = 11.84 RR21 pKa = 11.84 PVLPHH26 pKa = 4.66 VRR28 pKa = 11.84 LRR30 pKa = 11.84 RR31 pKa = 11.84 APRR34 pKa = 11.84 VRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 LPARR43 pKa = 11.84 RR44 pKa = 11.84 PPPEE48 pKa = 4.86 RR49 pKa = 11.84 PLPWRR54 pKa = 11.84 ARR56 pKa = 11.84 PGRR59 pKa = 11.84 HH60 pKa = 5.75 PRR62 pKa = 11.84 PVRR65 pKa = 11.84 PGRR68 pKa = 11.84 RR69 pKa = 11.84 PPRR72 pKa = 11.84 RR73 pKa = 11.84 ARR75 pKa = 11.84 HH76 pKa = 5.31 RR77 pKa = 11.84 PARR80 pKa = 11.84 PPPRR84 pKa = 11.84 TPPRR88 pKa = 11.84 HH89 pKa = 5.97 PPPVVRR95 pKa = 11.84 PLPHH99 pKa = 6.27 ARR101 pKa = 11.84 VRR103 pKa = 11.84 PRR105 pKa = 11.84 RR106 pKa = 11.84 PLRR109 pKa = 11.84 PARR112 pKa = 11.84 RR113 pKa = 11.84 RR114 pKa = 11.84 PPRR117 pKa = 11.84 VRR119 pKa = 11.84 RR120 pKa = 11.84 RR121 pKa = 11.84 AARR124 pKa = 11.84 PHH126 pKa = 6.41 RR127 pKa = 11.84 VPARR131 pKa = 11.84 PSRR134 pKa = 11.84 PRR136 pKa = 11.84 RR137 pKa = 11.84 RR138 pKa = 11.84 LRR140 pKa = 11.84 PRR142 pKa = 11.84 CRR144 pKa = 11.84 RR145 pKa = 11.84 PPRR148 pKa = 11.84 PRR150 pKa = 11.84 PLPSGCRR157 pKa = 11.84 APVTVASRR165 pKa = 11.84 VPAYY169 pKa = 9.82 RR170 pKa = 11.84 RR171 pKa = 11.84 RR172 pKa = 11.84 PVRR175 pKa = 11.84 PPVRR179 pKa = 11.84 VSRR182 pKa = 11.84 APATTRR188 pKa = 11.84 SRR190 pKa = 11.84 RR191 pKa = 11.84 ARR193 pKa = 11.84 EE194 pKa = 3.54 WVRR197 pKa = 11.84 PVRR200 pKa = 11.84 PVPVATRR207 pKa = 11.84 ARR209 pKa = 11.84 DD210 pKa = 3.29 RR211 pKa = 11.84 ATTRR215 pKa = 11.84 SARR218 pKa = 11.84 TRR220 pKa = 11.84 GCRR223 pKa = 11.84 VRR225 pKa = 11.84 SSGQGPPVPAAPVALVVPAGPVRR248 pKa = 11.84 PAPAVPVRR256 pKa = 11.84 VLPPVAPRR264 pKa = 11.84 VPAVRR269 pKa = 11.84 ARR271 pKa = 11.84 ARR273 pKa = 11.84 AA274 pKa = 3.28
Molecular weight: 31.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.509
IPC2_protein 11.199
IPC_protein 12.778
Toseland 12.939
ProMoST 13.437
Dawson 12.939
Bjellqvist 12.939
Wikipedia 13.408
Rodwell 12.427
Grimsley 12.969
Solomon 13.437
Lehninger 13.334
Nozaki 12.939
DTASelect 12.939
Thurlkill 12.939
EMBOSS 13.437
Sillero 12.939
Patrickios 12.135
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.197
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3555
0
3555
1213893
30
4235
341.5
36.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.384 ± 0.047
0.642 ± 0.008
6.683 ± 0.036
5.229 ± 0.036
2.623 ± 0.022
9.609 ± 0.037
2.188 ± 0.023
2.838 ± 0.03
1.733 ± 0.028
9.957 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.654 ± 0.016
1.598 ± 0.021
5.855 ± 0.031
2.644 ± 0.02
7.989 ± 0.044
5.477 ± 0.033
6.602 ± 0.058
9.988 ± 0.046
1.516 ± 0.021
1.794 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here