Panthera leo polyomavirus 1
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1CJP5|A0A2S1CJP5_9POLY 83T protein OS=Panthera leo polyomavirus 1 OX=2170405 GN=83T PE=4 SV=1
MM1 pKa = 7.46 FFILGMGALLAVLAEE16 pKa = 4.28 VFDD19 pKa = 4.55 LATAAGVGIDD29 pKa = 4.51 AILSGEE35 pKa = 4.16 ALATAEE41 pKa = 4.06 ALEE44 pKa = 4.3 AFVTNLVTLEE54 pKa = 4.21 GFTEE58 pKa = 4.44 AEE60 pKa = 3.93 ALAAAGITQEE70 pKa = 4.63 ALTAFNQFGAVFPQLYY86 pKa = 9.98 AALAVDD92 pKa = 4.34 SVAIGSALAASLAPYY107 pKa = 9.29 NYY109 pKa = 10.48 SYY111 pKa = 7.65 TTPIANLNMALIPYY125 pKa = 9.68 IGEE128 pKa = 3.87 LDD130 pKa = 3.41 IYY132 pKa = 10.77 FPGAMHH138 pKa = 7.42 IARR141 pKa = 11.84 FIHH144 pKa = 6.85 YY145 pKa = 9.6 IDD147 pKa = 3.75 PTNWAVDD154 pKa = 4.29 LYY156 pKa = 10.52 QALGRR161 pKa = 11.84 YY162 pKa = 7.21 VWEE165 pKa = 4.45 GLQRR169 pKa = 11.84 AGQRR173 pKa = 11.84 FLDD176 pKa = 3.78 NQRR179 pKa = 11.84 LQLEE183 pKa = 4.28 HH184 pKa = 6.68 ATRR187 pKa = 11.84 EE188 pKa = 4.0 LAQRR192 pKa = 11.84 GAQTAAEE199 pKa = 4.04 ALAQYY204 pKa = 10.03 FEE206 pKa = 4.14 NARR209 pKa = 11.84 WAISNFNPQNLYY221 pKa = 10.59 RR222 pKa = 11.84 GLEE225 pKa = 3.74 NYY227 pKa = 9.02 YY228 pKa = 10.53 RR229 pKa = 11.84 QLPPLNPIQRR239 pKa = 11.84 RR240 pKa = 11.84 QLDD243 pKa = 3.49 RR244 pKa = 11.84 RR245 pKa = 11.84 AGLPVPDD252 pKa = 3.66 RR253 pKa = 11.84 VLAIEE258 pKa = 5.16 DD259 pKa = 3.73 NVKK262 pKa = 10.54 SADD265 pKa = 3.59 YY266 pKa = 10.47 VEE268 pKa = 6.05 RR269 pKa = 11.84 YY270 pKa = 9.36 APPGGAFQRR279 pKa = 11.84 QTPDD283 pKa = 2.11 WMLPLILGLYY293 pKa = 10.41 GDD295 pKa = 5.31 INPSWGDD302 pKa = 3.24 TLTQLEE308 pKa = 4.98 KK309 pKa = 11.08 EE310 pKa = 4.69 DD311 pKa = 4.31 GPPSKK316 pKa = 10.22 KK317 pKa = 9.85 RR318 pKa = 11.84 KK319 pKa = 8.05 KK320 pKa = 9.17 TII322 pKa = 3.26
Molecular weight: 35.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.786
IPC2_protein 4.876
IPC_protein 4.774
Toseland 4.647
ProMoST 4.889
Dawson 4.724
Bjellqvist 4.876
Wikipedia 4.584
Rodwell 4.635
Grimsley 4.558
Solomon 4.724
Lehninger 4.673
Nozaki 4.838
DTASelect 4.978
Thurlkill 4.635
EMBOSS 4.609
Sillero 4.902
Patrickios 3.745
IPC_peptide 4.736
IPC2_peptide 4.902
IPC2.peptide.svr19 4.862
Protein with the highest isoelectric point:
>tr|A0A2S1CJQ6|A0A2S1CJQ6_9POLY 181T protein OS=Panthera leo polyomavirus 1 OX=2170405 GN=181T PE=4 SV=1
MM1 pKa = 7.6 DD2 pKa = 6.14 HH3 pKa = 7.11 ILTRR7 pKa = 11.84 EE8 pKa = 3.63 EE9 pKa = 4.28 SKK11 pKa = 11.2 RR12 pKa = 11.84 LMEE15 pKa = 4.97 LLNLPMTEE23 pKa = 4.1 YY24 pKa = 11.46 GNFPLMRR31 pKa = 11.84 RR32 pKa = 11.84 AFLKK36 pKa = 10.24 ACKK39 pKa = 9.45 ILHH42 pKa = 6.55 PDD44 pKa = 2.86 KK45 pKa = 11.28 GGNAEE50 pKa = 4.03 QAQEE54 pKa = 4.5 LISLYY59 pKa = 10.54 RR60 pKa = 11.84 KK61 pKa = 10.2 LEE63 pKa = 3.91 EE64 pKa = 4.73 SLPSLNPQEE73 pKa = 5.69 SFTTDD78 pKa = 2.75 QKK80 pKa = 11.17 KK81 pKa = 9.21 QEE83 pKa = 4.1 QEE85 pKa = 4.18 TQGLGCMLLLRR96 pKa = 11.84 MLYY99 pKa = 9.82 SLVWAGIYY107 pKa = 9.23 LLCLLLLDD115 pKa = 4.93 GNNSQFTLWLLEE127 pKa = 3.83 YY128 pKa = 10.12 LVWKK132 pKa = 10.24 ILMRR136 pKa = 11.84 GNPNPAPASSKK147 pKa = 9.23 EE148 pKa = 4.24 EE149 pKa = 3.96 KK150 pKa = 10.52 GNPPMTLPPAPKK162 pKa = 8.91 PRR164 pKa = 11.84 HH165 pKa = 5.92 RR166 pKa = 11.84 KK167 pKa = 8.53 SQLSKK172 pKa = 10.47 KK173 pKa = 9.03 KK174 pKa = 10.03 KK175 pKa = 8.68 KK176 pKa = 9.78 SRR178 pKa = 11.84 RR179 pKa = 11.84 TWW181 pKa = 2.86
Molecular weight: 20.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.946
IPC2_protein 8.946
IPC_protein 8.873
Toseland 9.78
ProMoST 9.399
Dawson 9.97
Bjellqvist 9.619
Wikipedia 10.101
Rodwell 10.482
Grimsley 10.014
Solomon 9.999
Lehninger 9.984
Nozaki 9.838
DTASelect 9.589
Thurlkill 9.838
EMBOSS 10.175
Sillero 9.897
Patrickios 10.072
IPC_peptide 9.999
IPC2_peptide 8.185
IPC2.peptide.svr19 7.862
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
3005
83
713
300.5
34.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.29 ± 1.034
2.762 ± 0.513
5.125 ± 0.409
7.488 ± 0.347
4.592 ± 0.447
5.358 ± 0.485
1.364 ± 0.148
5.125 ± 0.372
6.955 ± 0.749
11.78 ± 0.673
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.662 ± 0.342
4.992 ± 0.119
5.857 ± 0.663
4.759 ± 0.318
5.291 ± 0.347
5.691 ± 0.482
4.626 ± 0.269
4.393 ± 0.524
1.231 ± 0.167
3.661 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here