Streptococcus satellite phage Javan257

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZH98|A0A4D5ZH98_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan257 OX=2558594 GN=JavanS257_0003 PE=4 SV=1
MM1 pKa = 7.65AGADD5 pKa = 3.54LVEE8 pKa = 4.13VLEE11 pKa = 4.3DD12 pKa = 3.78LEE14 pKa = 4.34RR15 pKa = 11.84HH16 pKa = 5.68RR17 pKa = 11.84LEE19 pKa = 4.55QARR22 pKa = 11.84QDD24 pKa = 3.44VGLEE28 pKa = 3.71LGGWIFDD35 pKa = 4.45TIANGLYY42 pKa = 9.51TEE44 pKa = 4.35QEE46 pKa = 3.72TAYY49 pKa = 10.4FIRR52 pKa = 11.84LMFLHH57 pKa = 7.19GYY59 pKa = 10.15DD60 pKa = 4.34FEE62 pKa = 4.52QLTSLFSAIVKK73 pKa = 10.11RR74 pKa = 11.84IEE76 pKa = 3.78LASYY80 pKa = 10.39FLTTAKK86 pKa = 10.26KK87 pKa = 9.41IYY89 pKa = 10.03RR90 pKa = 11.84GVEE93 pKa = 3.95FGG95 pKa = 3.96

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZHK6|A0A4D5ZHK6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan257 OX=2558594 GN=JavanS257_0001 PE=4 SV=1
MM1 pKa = 7.61NIKK4 pKa = 9.79EE5 pKa = 4.17YY6 pKa = 10.64KK7 pKa = 9.74KK8 pKa = 10.0EE9 pKa = 3.81NGTIVYY15 pKa = 9.83RR16 pKa = 11.84SSVYY20 pKa = 10.72LGVDD24 pKa = 2.81RR25 pKa = 11.84LTGKK29 pKa = 10.3KK30 pKa = 10.27ARR32 pKa = 11.84TTVTASTKK40 pKa = 10.51KK41 pKa = 9.66GVKK44 pKa = 9.22VKK46 pKa = 10.69ARR48 pKa = 11.84EE49 pKa = 3.92AVNAFVANGHH59 pKa = 4.86TAKK62 pKa = 9.91TKK64 pKa = 9.13PTITTYY70 pKa = 11.05KK71 pKa = 10.28EE72 pKa = 4.76LVALWWDD79 pKa = 3.7SYY81 pKa = 11.95KK82 pKa = 10.1NTIKK86 pKa = 10.73PNSQQSMEE94 pKa = 4.61GIVRR98 pKa = 11.84LHH100 pKa = 6.54ILPVFGDD107 pKa = 3.73YY108 pKa = 10.61KK109 pKa = 10.47LSKK112 pKa = 9.21ITTPVIQQQVNKK124 pKa = 8.49WANAANKK131 pKa = 9.73GEE133 pKa = 4.01KK134 pKa = 9.53GAYY137 pKa = 9.7ANYY140 pKa = 10.84SFLNNINKK148 pKa = 10.23RR149 pKa = 11.84ILQYY153 pKa = 11.18AVTMQLIPYY162 pKa = 8.95NPARR166 pKa = 11.84DD167 pKa = 3.67VIVPRR172 pKa = 11.84KK173 pKa = 9.79NQDD176 pKa = 2.91KK177 pKa = 9.88EE178 pKa = 4.23KK179 pKa = 10.46KK180 pKa = 9.72IKK182 pKa = 10.4FFSNQEE188 pKa = 3.75LKK190 pKa = 10.86QFLTYY195 pKa = 10.41LDD197 pKa = 4.64KK198 pKa = 11.23LDD200 pKa = 3.54QSSYY204 pKa = 11.2EE205 pKa = 3.9NLFDD209 pKa = 3.87FVLYY213 pKa = 10.64KK214 pKa = 10.54FLLATGCRR222 pKa = 11.84IGEE225 pKa = 4.06ALALEE230 pKa = 4.49WSDD233 pKa = 4.08IDD235 pKa = 4.95LKK237 pKa = 11.39NGTVNVTKK245 pKa = 10.03TLNRR249 pKa = 11.84YY250 pKa = 9.9LEE252 pKa = 4.4TNTPKK257 pKa = 10.74SKK259 pKa = 10.8AGLRR263 pKa = 11.84NVEE266 pKa = 3.64IDD268 pKa = 3.19KK269 pKa = 9.98ATVSLLKK276 pKa = 10.19QYY278 pKa = 10.95KK279 pKa = 9.65KK280 pKa = 10.33RR281 pKa = 11.84QQLHH285 pKa = 5.53AWQLGRR291 pKa = 11.84TEE293 pKa = 4.19TVVFSVFTNKK303 pKa = 9.42YY304 pKa = 9.73AHH306 pKa = 6.74ACLLRR311 pKa = 11.84KK312 pKa = 9.63RR313 pKa = 11.84LQQHH317 pKa = 6.02FKK319 pKa = 10.63SAGVPDD325 pKa = 3.94ISFHH329 pKa = 5.86GFRR332 pKa = 11.84HH333 pKa = 4.4THH335 pKa = 4.65ATIMLYY341 pKa = 10.62AGIEE345 pKa = 4.18AKK347 pKa = 10.42DD348 pKa = 3.35LQYY351 pKa = 11.55RR352 pKa = 11.84LGHH355 pKa = 6.02SNISMTLNTYY365 pKa = 8.67VHH367 pKa = 6.71ATQEE371 pKa = 4.37GAKK374 pKa = 9.47KK375 pKa = 10.08AVSIFEE381 pKa = 4.16TAISNLL387 pKa = 3.59

Molecular weight: 44.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2552

52

474

182.3

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.132 ± 0.574

0.274 ± 0.119

5.603 ± 0.44

8.307 ± 0.717

3.683 ± 0.36

5.133 ± 0.421

2.038 ± 0.288

6.818 ± 0.43

9.248 ± 0.444

9.13 ± 0.66

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.724 ± 0.308

4.741 ± 0.488

2.547 ± 0.499

5.133 ± 0.454

4.741 ± 0.498

5.172 ± 0.357

6.701 ± 0.466

5.408 ± 0.419

1.097 ± 0.187

5.368 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski