Alkalibacillus haloalkaliphilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales;

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2984 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A511W2N4|A0A511W2N4_9BACI Ferredoxin--NADP reductase OS=Alkalibacillus haloalkaliphilus OX=94136 GN=yumC_2 PE=3 SV=1
MM1 pKa = 7.35FKK3 pKa = 10.15HH4 pKa = 6.23RR5 pKa = 11.84KK6 pKa = 8.56IIYY9 pKa = 8.69GLSLIAIAILLAGCNGSEE27 pKa = 3.93ASEE30 pKa = 3.86IDD32 pKa = 3.42SEE34 pKa = 4.4INIDD38 pKa = 3.65IEE40 pKa = 4.09HH41 pKa = 6.89FEE43 pKa = 4.08YY44 pKa = 10.78TNQDD48 pKa = 3.63EE49 pKa = 4.67EE50 pKa = 5.14TVTLDD55 pKa = 4.76DD56 pKa = 5.28LEE58 pKa = 4.36GTYY61 pKa = 9.05WVANFIFTNCNTVCPPMTSNMANLQQEE88 pKa = 5.23AEE90 pKa = 4.22DD91 pKa = 3.51QDD93 pKa = 4.27LDD95 pKa = 3.63VRR97 pKa = 11.84FVSFSVDD104 pKa = 3.35PEE106 pKa = 4.04YY107 pKa = 11.15DD108 pKa = 3.72DD109 pKa = 5.98PDD111 pKa = 3.95TLKK114 pKa = 10.18TFANQFDD121 pKa = 4.39ADD123 pKa = 3.79YY124 pKa = 11.1SNWDD128 pKa = 3.32FLTGYY133 pKa = 7.31SQKK136 pKa = 10.49EE137 pKa = 4.06IEE139 pKa = 4.52SFAASSFKK147 pKa = 11.1LLVSKK152 pKa = 11.04VDD154 pKa = 3.32GHH156 pKa = 6.4DD157 pKa = 3.54QVSHH161 pKa = 5.52GTSFFIVDD169 pKa = 3.92PEE171 pKa = 4.11GTAINNYY178 pKa = 9.89SGTSSEE184 pKa = 4.57EE185 pKa = 3.4MDD187 pKa = 5.38QIIDD191 pKa = 4.14DD192 pKa = 4.66LNILLDD198 pKa = 3.63

Molecular weight: 22.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A511W0S8|A0A511W0S8_9BACI UPF0033 protein YrkI OS=Alkalibacillus haloalkaliphilus OX=94136 GN=yrkI_2 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.35QPNNRR10 pKa = 11.84KK11 pKa = 9.38RR12 pKa = 11.84NKK14 pKa = 8.76VHH16 pKa = 6.64GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2984

0

2984

843071

39

3057

282.5

31.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.401 ± 0.041

0.588 ± 0.013

5.913 ± 0.047

8.404 ± 0.06

4.466 ± 0.041

6.65 ± 0.039

2.27 ± 0.025

7.47 ± 0.048

5.814 ± 0.051

9.542 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.022

4.586 ± 0.031

3.457 ± 0.026

4.275 ± 0.035

3.965 ± 0.032

5.9 ± 0.033

5.364 ± 0.03

7.521 ± 0.036

0.975 ± 0.016

3.699 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski