Hepatocystis sp. ex Piliocolobus tephrosceles

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Hepatocystis; unclassified Hepatocystis

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653H9B9|A0A653H9B9_9APIC Uncharacterized protein (Fragment) OS=Hepatocystis sp. ex Piliocolobus tephrosceles OX=2600580 GN=HEP_00467500 PE=4 SV=1
NN1 pKa = 7.39NSGNNSGNNSGNNSGNNSDD20 pKa = 4.39NDD22 pKa = 3.82SSSLSSCTDD31 pKa = 3.48TSCTEE36 pKa = 3.67IYY38 pKa = 8.96HH39 pKa = 5.8TFFNFNEE46 pKa = 4.42KK47 pKa = 9.84KK48 pKa = 10.23HH49 pKa = 6.04NNQINNRR56 pKa = 11.84KK57 pKa = 9.23VCDD60 pKa = 3.85RR61 pKa = 11.84YY62 pKa = 11.43DD63 pKa = 4.83NYY65 pKa = 11.16NQWIGDD71 pKa = 4.05NISDD75 pKa = 4.52NISDD79 pKa = 4.43NISDD83 pKa = 4.68KK84 pKa = 11.11ISDD87 pKa = 4.27NISDD91 pKa = 4.69KK92 pKa = 11.11ISDD95 pKa = 4.28NISDD99 pKa = 4.53NISDD103 pKa = 4.4NISYY107 pKa = 7.67DD108 pKa = 3.46TPYY111 pKa = 9.12NTPYY115 pKa = 10.82NITDD119 pKa = 3.72EE120 pKa = 4.9ASHH123 pKa = 5.33NTPYY127 pKa = 8.04EE128 pKa = 4.14TPNNIPEE135 pKa = 4.38DD136 pKa = 3.83VLL138 pKa = 4.25

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653H589|A0A653H589_9APIC Uncharacterized protein OS=Hepatocystis sp. ex Piliocolobus tephrosceles OX=2600580 GN=HEP_00324500 PE=4 SV=1
MM1 pKa = 7.69GYY3 pKa = 8.97IQNVHH8 pKa = 6.55PKK10 pKa = 10.07NYY12 pKa = 10.22GPGSRR17 pKa = 11.84QCRR20 pKa = 11.84VCSNKK25 pKa = 9.7HH26 pKa = 5.32AIIRR30 pKa = 11.84KK31 pKa = 8.76YY32 pKa = 10.56NINICRR38 pKa = 11.84QCFRR42 pKa = 11.84EE43 pKa = 3.88RR44 pKa = 11.84ADD46 pKa = 3.23IIGFKK51 pKa = 10.4KK52 pKa = 10.41FRR54 pKa = 3.87

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5281

0

5281

3455889

31

9600

654.4

76.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.262 ± 0.019

1.736 ± 0.013

5.654 ± 0.025

6.379 ± 0.046

4.333 ± 0.029

3.425 ± 0.029

2.052 ± 0.01

8.954 ± 0.03

11.546 ± 0.037

7.692 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.894 ± 0.012

13.986 ± 0.086

1.889 ± 0.02

2.84 ± 0.019

2.449 ± 0.016

7.466 ± 0.037

5.506 ± 0.029

4.191 ± 0.021

0.445 ± 0.006

5.285 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski