Vibrio phage Quinn
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 180 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M9Z212|A0A6M9Z212_9CAUD Uncharacterized protein OS=Vibrio phage Quinn OX=2736265 GN=QUINN_11 PE=4 SV=1
MM1 pKa = 7.13 VKK3 pKa = 10.55 VIITFGCYY11 pKa = 10.13 LEE13 pKa = 4.99 TCHH16 pKa = 7.0 EE17 pKa = 4.31 MVLVTNRR24 pKa = 11.84 DD25 pKa = 3.48 TLTEE29 pKa = 4.16 AEE31 pKa = 4.05 HH32 pKa = 7.16 DD33 pKa = 4.33 KK34 pKa = 11.26 INSEE38 pKa = 4.19 VVYY41 pKa = 10.08 PMAIEE46 pKa = 4.27 TTDD49 pKa = 2.79 GWVGSNGFCEE59 pKa = 4.76 DD60 pKa = 4.15 LDD62 pKa = 3.98 DD63 pKa = 6.27 AEE65 pKa = 5.79 DD66 pKa = 3.58 IEE68 pKa = 4.75 EE69 pKa = 4.22 AVEE72 pKa = 5.21 DD73 pKa = 3.63 EE74 pKa = 4.05 AFYY77 pKa = 11.09 YY78 pKa = 10.45 SEE80 pKa = 4.75 IYY82 pKa = 10.57 DD83 pKa = 3.92 PEE85 pKa = 4.17 KK86 pKa = 10.82 HH87 pKa = 7.15 DD88 pKa = 4.23 GFLMGCQDD96 pKa = 3.06 EE97 pKa = 4.33 WQIIEE102 pKa = 4.48 EE103 pKa = 4.27 IEE105 pKa = 3.94 LL106 pKa = 3.79
Molecular weight: 12.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.831
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.49
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.554
Rodwell 3.516
Grimsley 3.414
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.91
Thurlkill 3.541
EMBOSS 3.567
Sillero 3.795
Patrickios 0.299
IPC_peptide 3.63
IPC2_peptide 3.77
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A6M9Z3Q3|A0A6M9Z3Q3_9CAUD Uncharacterized protein OS=Vibrio phage Quinn OX=2736265 GN=QUINN_190 PE=4 SV=1
MM1 pKa = 7.8 AKK3 pKa = 9.23 RR4 pKa = 11.84 SKK6 pKa = 10.37 MIGIKK11 pKa = 9.91 MSDD14 pKa = 3.72 RR15 pKa = 11.84 TRR17 pKa = 11.84 KK18 pKa = 8.26 MAGEE22 pKa = 3.94 YY23 pKa = 10.34 GIEE26 pKa = 3.78 ILEE29 pKa = 4.0 QRR31 pKa = 11.84 RR32 pKa = 11.84 SGLAKK37 pKa = 10.62 VLGLLARR44 pKa = 11.84 GTRR47 pKa = 11.84 VSNHH51 pKa = 5.19 PRR53 pKa = 11.84 AVEE56 pKa = 3.66 YY57 pKa = 9.12 TAAQFGLL64 pKa = 3.85
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.955
IPC_protein 10.965
Toseland 11.125
ProMoST 11.125
Dawson 11.169
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.316
Grimsley 11.213
Solomon 11.433
Lehninger 11.374
Nozaki 11.096
DTASelect 10.965
Thurlkill 11.111
EMBOSS 11.55
Sillero 11.125
Patrickios 11.082
IPC_peptide 11.433
IPC2_peptide 10.072
IPC2.peptide.svr19 8.534
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
180
0
180
35617
29
1018
197.9
22.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.699 ± 0.284
1.202 ± 0.09
6.354 ± 0.164
6.999 ± 0.256
4.032 ± 0.123
6.547 ± 0.176
2.075 ± 0.107
6.143 ± 0.118
7.199 ± 0.164
8.358 ± 0.198
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.757 ± 0.109
5.149 ± 0.151
3.428 ± 0.152
3.549 ± 0.15
4.4 ± 0.138
6.152 ± 0.267
6.084 ± 0.187
6.539 ± 0.155
1.393 ± 0.072
3.942 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here