Amino acid dipepetide frequency for Vibrio phage Quinn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.289AlaAla: 6.289 ± 0.896
0.758AlaCys: 0.758 ± 0.152
4.829AlaAsp: 4.829 ± 0.419
5.531AlaGlu: 5.531 ± 0.518
2.92AlaPhe: 2.92 ± 0.283
4.352AlaGly: 4.352 ± 0.343
1.095AlaHis: 1.095 ± 0.183
4.324AlaIle: 4.324 ± 0.333
7.019AlaLys: 7.019 ± 0.623
6.542AlaLeu: 6.542 ± 0.416
2.246AlaMet: 2.246 ± 0.255
3.875AlaAsn: 3.875 ± 0.374
2.415AlaPro: 2.415 ± 0.285
3.032AlaGln: 3.032 ± 0.305
3.566AlaArg: 3.566 ± 0.313
5.335AlaSer: 5.335 ± 0.607
4.717AlaThr: 4.717 ± 0.466
4.296AlaVal: 4.296 ± 0.38
1.067AlaTrp: 1.067 ± 0.171
2.78AlaTyr: 2.78 ± 0.272
0.0AlaXaa: 0.0 ± 0.0
Cys
0.842CysAla: 0.842 ± 0.176
0.112CysCys: 0.112 ± 0.055
0.758CysAsp: 0.758 ± 0.159
0.955CysGlu: 0.955 ± 0.154
0.421CysPhe: 0.421 ± 0.108
0.702CysGly: 0.702 ± 0.153
0.337CysHis: 0.337 ± 0.124
0.646CysIle: 0.646 ± 0.15
0.87CysLys: 0.87 ± 0.155
0.758CysLeu: 0.758 ± 0.176
0.309CysMet: 0.309 ± 0.088
0.674CysAsn: 0.674 ± 0.138
0.477CysPro: 0.477 ± 0.111
0.562CysGln: 0.562 ± 0.09
0.562CysArg: 0.562 ± 0.119
0.786CysSer: 0.786 ± 0.185
0.73CysThr: 0.73 ± 0.136
0.87CysVal: 0.87 ± 0.177
0.14CysTrp: 0.14 ± 0.055
0.505CysTyr: 0.505 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
4.661AspAla: 4.661 ± 0.374
0.702AspCys: 0.702 ± 0.176
3.566AspAsp: 3.566 ± 0.355
4.605AspGlu: 4.605 ± 0.424
3.145AspPhe: 3.145 ± 0.305
4.38AspGly: 4.38 ± 0.392
0.87AspHis: 0.87 ± 0.155
4.436AspIle: 4.436 ± 0.319
4.717AspLys: 4.717 ± 0.368
4.998AspLeu: 4.998 ± 0.364
1.881AspMet: 1.881 ± 0.248
3.538AspAsn: 3.538 ± 0.303
2.33AspPro: 2.33 ± 0.315
1.488AspGln: 1.488 ± 0.193
2.639AspArg: 2.639 ± 0.308
3.706AspSer: 3.706 ± 0.379
4.268AspThr: 4.268 ± 0.371
4.184AspVal: 4.184 ± 0.297
1.123AspTrp: 1.123 ± 0.154
2.302AspTyr: 2.302 ± 0.272
0.0AspXaa: 0.0 ± 0.0
Glu
6.345GluAla: 6.345 ± 0.525
1.039GluCys: 1.039 ± 0.207
4.015GluAsp: 4.015 ± 0.348
4.464GluGlu: 4.464 ± 0.444
2.78GluPhe: 2.78 ± 0.271
4.155GluGly: 4.155 ± 0.378
1.657GluHis: 1.657 ± 0.208
4.689GluIle: 4.689 ± 0.358
4.801GluLys: 4.801 ± 0.435
6.991GluLeu: 6.991 ± 0.438
2.106GluMet: 2.106 ± 0.267
3.454GluAsn: 3.454 ± 0.293
1.993GluPro: 1.993 ± 0.252
2.948GluGln: 2.948 ± 0.352
2.752GluArg: 2.752 ± 0.307
2.78GluSer: 2.78 ± 0.319
3.482GluThr: 3.482 ± 0.317
5.222GluVal: 5.222 ± 0.449
1.151GluTrp: 1.151 ± 0.171
3.173GluTyr: 3.173 ± 0.339
0.0GluXaa: 0.0 ± 0.0
Phe
2.695PheAla: 2.695 ± 0.32
0.73PheCys: 0.73 ± 0.14
3.201PheAsp: 3.201 ± 0.317
2.92PheGlu: 2.92 ± 0.264
1.6PhePhe: 1.6 ± 0.231
2.639PheGly: 2.639 ± 0.249
1.263PheHis: 1.263 ± 0.181
2.808PheIle: 2.808 ± 0.272
2.92PheLys: 2.92 ± 0.284
2.471PheLeu: 2.471 ± 0.313
1.039PheMet: 1.039 ± 0.138
2.387PheAsn: 2.387 ± 0.256
1.432PhePro: 1.432 ± 0.191
1.095PheGln: 1.095 ± 0.166
1.685PheArg: 1.685 ± 0.175
2.499PheSer: 2.499 ± 0.267
2.555PheThr: 2.555 ± 0.298
2.302PheVal: 2.302 ± 0.262
0.646PheTrp: 0.646 ± 0.133
1.432PheTyr: 1.432 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
4.212GlyAla: 4.212 ± 0.375
0.955GlyCys: 0.955 ± 0.185
4.829GlyAsp: 4.829 ± 0.455
4.099GlyGlu: 4.099 ± 0.345
3.201GlyPhe: 3.201 ± 0.272
3.482GlyGly: 3.482 ± 0.392
1.095GlyHis: 1.095 ± 0.154
4.352GlyIle: 4.352 ± 0.337
5.026GlyLys: 5.026 ± 0.369
5.222GlyLeu: 5.222 ± 0.409
1.881GlyMet: 1.881 ± 0.212
3.313GlyAsn: 3.313 ± 0.365
0.505GlyPro: 0.505 ± 0.102
2.05GlyGln: 2.05 ± 0.235
3.173GlyArg: 3.173 ± 0.273
4.24GlySer: 4.24 ± 0.481
4.829GlyThr: 4.829 ± 0.408
4.352GlyVal: 4.352 ± 0.293
1.572GlyTrp: 1.572 ± 0.215
3.088GlyTyr: 3.088 ± 0.333
0.0GlyXaa: 0.0 ± 0.0
His
1.151HisAla: 1.151 ± 0.172
0.309HisCys: 0.309 ± 0.095
1.404HisAsp: 1.404 ± 0.225
1.432HisGlu: 1.432 ± 0.199
0.758HisPhe: 0.758 ± 0.14
1.6HisGly: 1.6 ± 0.202
0.618HisHis: 0.618 ± 0.118
1.348HisIle: 1.348 ± 0.188
1.46HisLys: 1.46 ± 0.207
2.19HisLeu: 2.19 ± 0.256
0.674HisMet: 0.674 ± 0.159
0.983HisAsn: 0.983 ± 0.153
0.898HisPro: 0.898 ± 0.162
0.505HisGln: 0.505 ± 0.115
1.039HisArg: 1.039 ± 0.149
0.983HisSer: 0.983 ± 0.169
1.151HisThr: 1.151 ± 0.193
1.123HisVal: 1.123 ± 0.196
0.253HisTrp: 0.253 ± 0.077
0.87HisTyr: 0.87 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
4.436IleAla: 4.436 ± 0.364
0.674IleCys: 0.674 ± 0.169
3.987IleAsp: 3.987 ± 0.442
5.531IleGlu: 5.531 ± 0.45
1.797IlePhe: 1.797 ± 0.216
3.538IleGly: 3.538 ± 0.295
1.263IleHis: 1.263 ± 0.178
3.65IleIle: 3.65 ± 0.332
4.885IleLys: 4.885 ± 0.381
4.38IleLeu: 4.38 ± 0.308
1.713IleMet: 1.713 ± 0.19
3.678IleAsn: 3.678 ± 0.326
2.302IlePro: 2.302 ± 0.266
2.471IleGln: 2.471 ± 0.257
2.948IleArg: 2.948 ± 0.267
4.268IleSer: 4.268 ± 0.332
3.819IleThr: 3.819 ± 0.362
3.341IleVal: 3.341 ± 0.334
0.646IleTrp: 0.646 ± 0.125
2.106IleTyr: 2.106 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
6.542LysAla: 6.542 ± 0.558
0.702LysCys: 0.702 ± 0.15
4.015LysAsp: 4.015 ± 0.359
5.222LysGlu: 5.222 ± 0.457
3.088LysPhe: 3.088 ± 0.324
4.52LysGly: 4.52 ± 0.301
1.6LysHis: 1.6 ± 0.187
4.38LysIle: 4.38 ± 0.321
4.155LysLys: 4.155 ± 0.365
7.412LysLeu: 7.412 ± 0.498
2.387LysMet: 2.387 ± 0.261
2.864LysAsn: 2.864 ± 0.255
3.117LysPro: 3.117 ± 0.411
2.78LysGln: 2.78 ± 0.251
2.976LysArg: 2.976 ± 0.296
3.706LysSer: 3.706 ± 0.294
4.015LysThr: 4.015 ± 0.361
4.633LysVal: 4.633 ± 0.337
0.786LysTrp: 0.786 ± 0.134
3.088LysTyr: 3.088 ± 0.316
0.0LysXaa: 0.0 ± 0.0
Leu
7.525LeuAla: 7.525 ± 0.502
0.786LeuCys: 0.786 ± 0.154
6.458LeuAsp: 6.458 ± 0.426
6.458LeuGlu: 6.458 ± 0.44
2.471LeuPhe: 2.471 ± 0.269
5.868LeuGly: 5.868 ± 0.403
1.797LeuHis: 1.797 ± 0.24
4.857LeuIle: 4.857 ± 0.342
4.998LeuLys: 4.998 ± 0.48
6.233LeuLeu: 6.233 ± 0.47
2.218LeuMet: 2.218 ± 0.217
3.79LeuAsn: 3.79 ± 0.329
3.482LeuPro: 3.482 ± 0.344
3.229LeuGln: 3.229 ± 0.305
3.847LeuArg: 3.847 ± 0.293
4.97LeuSer: 4.97 ± 0.34
4.717LeuThr: 4.717 ± 0.406
6.486LeuVal: 6.486 ± 0.389
0.814LeuTrp: 0.814 ± 0.172
2.583LeuTyr: 2.583 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.358MetAla: 2.358 ± 0.26
0.393MetCys: 0.393 ± 0.082
1.544MetAsp: 1.544 ± 0.207
1.965MetGlu: 1.965 ± 0.243
1.263MetPhe: 1.263 ± 0.198
1.516MetGly: 1.516 ± 0.165
0.618MetHis: 0.618 ± 0.133
1.572MetIle: 1.572 ± 0.237
2.358MetLys: 2.358 ± 0.27
2.527MetLeu: 2.527 ± 0.27
0.674MetMet: 0.674 ± 0.155
1.376MetAsn: 1.376 ± 0.205
1.235MetPro: 1.235 ± 0.205
0.87MetGln: 0.87 ± 0.163
1.263MetArg: 1.263 ± 0.189
1.741MetSer: 1.741 ± 0.181
1.6MetThr: 1.6 ± 0.186
1.572MetVal: 1.572 ± 0.209
0.253MetTrp: 0.253 ± 0.084
0.87MetTyr: 0.87 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
3.819AsnAla: 3.819 ± 0.331
0.646AsnCys: 0.646 ± 0.141
2.134AsnAsp: 2.134 ± 0.24
2.948AsnGlu: 2.948 ± 0.259
1.6AsnPhe: 1.6 ± 0.227
3.959AsnGly: 3.959 ± 0.309
1.039AsnHis: 1.039 ± 0.207
3.538AsnIle: 3.538 ± 0.296
4.633AsnLys: 4.633 ± 0.385
4.24AsnLeu: 4.24 ± 0.337
1.263AsnMet: 1.263 ± 0.181
2.387AsnAsn: 2.387 ± 0.26
2.499AsnPro: 2.499 ± 0.262
1.46AsnGln: 1.46 ± 0.226
2.106AsnArg: 2.106 ± 0.249
3.959AsnSer: 3.959 ± 0.41
3.117AsnThr: 3.117 ± 0.354
3.285AsnVal: 3.285 ± 0.3
0.73AsnTrp: 0.73 ± 0.146
2.134AsnTyr: 2.134 ± 0.25
0.0AsnXaa: 0.0 ± 0.0
Pro
2.274ProAla: 2.274 ± 0.294
0.421ProCys: 0.421 ± 0.12
2.387ProAsp: 2.387 ± 0.259
3.117ProGlu: 3.117 ± 0.289
1.376ProPhe: 1.376 ± 0.186
1.909ProGly: 1.909 ± 0.302
0.702ProHis: 0.702 ± 0.121
2.106ProIle: 2.106 ± 0.224
1.965ProLys: 1.965 ± 0.249
2.555ProLeu: 2.555 ± 0.297
1.067ProMet: 1.067 ± 0.188
2.19ProAsn: 2.19 ± 0.312
0.842ProPro: 0.842 ± 0.205
1.179ProGln: 1.179 ± 0.194
1.432ProArg: 1.432 ± 0.2
2.106ProSer: 2.106 ± 0.283
2.218ProThr: 2.218 ± 0.281
2.555ProVal: 2.555 ± 0.268
0.449ProTrp: 0.449 ± 0.11
1.432ProTyr: 1.432 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
3.173GlnAla: 3.173 ± 0.33
0.533GlnCys: 0.533 ± 0.115
2.162GlnAsp: 2.162 ± 0.244
2.33GlnGlu: 2.33 ± 0.283
1.488GlnPhe: 1.488 ± 0.164
2.415GlnGly: 2.415 ± 0.257
0.674GlnHis: 0.674 ± 0.13
2.33GlnIle: 2.33 ± 0.247
2.078GlnLys: 2.078 ± 0.237
3.566GlnLeu: 3.566 ± 0.352
1.067GlnMet: 1.067 ± 0.171
1.095GlnAsn: 1.095 ± 0.187
0.983GlnPro: 0.983 ± 0.157
1.179GlnGln: 1.179 ± 0.278
1.544GlnArg: 1.544 ± 0.239
2.134GlnSer: 2.134 ± 0.348
2.106GlnThr: 2.106 ± 0.228
2.639GlnVal: 2.639 ± 0.257
0.533GlnTrp: 0.533 ± 0.132
1.46GlnTyr: 1.46 ± 0.167
0.0GlnXaa: 0.0 ± 0.0
Arg
3.369ArgAla: 3.369 ± 0.329
0.421ArgCys: 0.421 ± 0.107
3.229ArgAsp: 3.229 ± 0.355
2.752ArgGlu: 2.752 ± 0.318
1.853ArgPhe: 1.853 ± 0.216
3.117ArgGly: 3.117 ± 0.275
0.927ArgHis: 0.927 ± 0.184
2.864ArgIle: 2.864 ± 0.266
3.145ArgLys: 3.145 ± 0.33
3.566ArgLeu: 3.566 ± 0.338
1.235ArgMet: 1.235 ± 0.199
2.443ArgAsn: 2.443 ± 0.299
1.46ArgPro: 1.46 ± 0.213
1.488ArgGln: 1.488 ± 0.19
1.993ArgArg: 1.993 ± 0.219
2.415ArgSer: 2.415 ± 0.258
2.246ArgThr: 2.246 ± 0.246
3.06ArgVal: 3.06 ± 0.265
0.562ArgTrp: 0.562 ± 0.121
1.853ArgTyr: 1.853 ± 0.216
0.0ArgXaa: 0.0 ± 0.0
Ser
4.52SerAla: 4.52 ± 0.526
0.618SerCys: 0.618 ± 0.143
3.482SerAsp: 3.482 ± 0.359
3.594SerGlu: 3.594 ± 0.403
2.583SerPhe: 2.583 ± 0.274
5.756SerGly: 5.756 ± 0.583
1.123SerHis: 1.123 ± 0.186
3.341SerIle: 3.341 ± 0.318
4.801SerLys: 4.801 ± 0.358
5.335SerLeu: 5.335 ± 0.478
1.572SerMet: 1.572 ± 0.201
3.425SerAsn: 3.425 ± 0.337
2.078SerPro: 2.078 ± 0.241
2.022SerGln: 2.022 ± 0.259
2.302SerArg: 2.302 ± 0.26
4.015SerSer: 4.015 ± 0.495
3.875SerThr: 3.875 ± 0.354
4.043SerVal: 4.043 ± 0.356
0.842SerTrp: 0.842 ± 0.182
2.19SerTyr: 2.19 ± 0.26
0.0SerXaa: 0.0 ± 0.0
Thr
4.661ThrAla: 4.661 ± 0.404
0.618ThrCys: 0.618 ± 0.134
3.65ThrAsp: 3.65 ± 0.335
3.79ThrGlu: 3.79 ± 0.328
2.892ThrPhe: 2.892 ± 0.304
4.324ThrGly: 4.324 ± 0.361
1.32ThrHis: 1.32 ± 0.195
3.987ThrIle: 3.987 ± 0.354
4.184ThrLys: 4.184 ± 0.387
4.773ThrLeu: 4.773 ± 0.415
1.123ThrMet: 1.123 ± 0.178
3.425ThrAsn: 3.425 ± 0.364
2.302ThrPro: 2.302 ± 0.302
2.218ThrGln: 2.218 ± 0.251
2.415ThrArg: 2.415 ± 0.256
3.931ThrSer: 3.931 ± 0.487
3.847ThrThr: 3.847 ± 0.453
3.65ThrVal: 3.65 ± 0.313
0.898ThrTrp: 0.898 ± 0.136
2.808ThrTyr: 2.808 ± 0.234
0.0ThrXaa: 0.0 ± 0.0
Val
4.633ValAla: 4.633 ± 0.403
0.702ValCys: 0.702 ± 0.138
4.127ValAsp: 4.127 ± 0.327
4.773ValGlu: 4.773 ± 0.363
3.004ValPhe: 3.004 ± 0.274
3.706ValGly: 3.706 ± 0.34
1.292ValHis: 1.292 ± 0.177
3.117ValIle: 3.117 ± 0.305
4.717ValLys: 4.717 ± 0.38
4.885ValLeu: 4.885 ± 0.362
1.432ValMet: 1.432 ± 0.213
3.594ValAsn: 3.594 ± 0.315
2.134ValPro: 2.134 ± 0.274
2.302ValGln: 2.302 ± 0.223
3.397ValArg: 3.397 ± 0.273
5.026ValSer: 5.026 ± 0.389
4.436ValThr: 4.436 ± 0.366
4.857ValVal: 4.857 ± 0.433
0.758ValTrp: 0.758 ± 0.139
2.499ValTyr: 2.499 ± 0.255
0.0ValXaa: 0.0 ± 0.0
Trp
1.179TrpAla: 1.179 ± 0.194
0.253TrpCys: 0.253 ± 0.076
1.067TrpAsp: 1.067 ± 0.157
0.646TrpGlu: 0.646 ± 0.101
0.505TrpPhe: 0.505 ± 0.13
1.011TrpGly: 1.011 ± 0.134
0.533TrpHis: 0.533 ± 0.125
0.59TrpIle: 0.59 ± 0.16
0.955TrpLys: 0.955 ± 0.178
1.263TrpLeu: 1.263 ± 0.205
0.449TrpMet: 0.449 ± 0.119
0.674TrpAsn: 0.674 ± 0.154
0.281TrpPro: 0.281 ± 0.127
0.59TrpGln: 0.59 ± 0.117
0.674TrpArg: 0.674 ± 0.142
0.898TrpSer: 0.898 ± 0.2
0.505TrpThr: 0.505 ± 0.113
1.067TrpVal: 1.067 ± 0.169
0.197TrpTrp: 0.197 ± 0.057
0.59TrpTyr: 0.59 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.302TyrAla: 2.302 ± 0.229
0.646TyrCys: 0.646 ± 0.132
2.695TyrAsp: 2.695 ± 0.297
2.864TyrGlu: 2.864 ± 0.3
1.572TyrPhe: 1.572 ± 0.185
2.527TyrGly: 2.527 ± 0.237
0.927TyrHis: 0.927 ± 0.141
2.499TyrIle: 2.499 ± 0.247
2.302TyrLys: 2.302 ± 0.252
3.678TyrLeu: 3.678 ± 0.362
1.207TyrMet: 1.207 ± 0.183
2.33TyrAsn: 2.33 ± 0.263
1.516TyrPro: 1.516 ± 0.187
2.022TyrGln: 2.022 ± 0.254
1.685TyrArg: 1.685 ± 0.175
2.022TyrSer: 2.022 ± 0.266
2.611TyrThr: 2.611 ± 0.284
1.853TyrVal: 1.853 ± 0.254
0.505TyrTrp: 0.505 ± 0.121
1.657TyrTyr: 1.657 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 180 proteins (35617 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski