Blumeria graminis f. sp. hordei (strain DH14) (Barley powdery mildew) (Oidium monilioides f. sp. hordei)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Blumeria; Blumeria hordei

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6486 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N1J5L1|N1J5L1_BLUG1 Pre-mRNA processing splicing factor 8 OS=Blumeria graminis f. sp. hordei (strain DH14) OX=546991 GN=BGHDH14_bgh06124 PE=4 SV=1
MM1 pKa = 7.94WINFSVTLAISWLILQVSCDD21 pKa = 4.94DD22 pKa = 4.35IPDD25 pKa = 3.6SDD27 pKa = 4.2MYY29 pKa = 11.49LPDD32 pKa = 3.52GTNGFVCDD40 pKa = 4.18LDD42 pKa = 4.93FYY44 pKa = 11.35SIDD47 pKa = 3.46HH48 pKa = 5.63VRR50 pKa = 11.84EE51 pKa = 3.74VAKK54 pKa = 10.84

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N1J4Q9|N1J4Q9_BLUG1 E3 ubiquitin-protein ligase listerin OS=Blumeria graminis f. sp. hordei (strain DH14) OX=546991 GN=BGHDH14_bgh06697 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.95RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.12KK15 pKa = 9.81KK16 pKa = 9.49RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 7.5TRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.05

Molecular weight: 3.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6452

34

6486

3136009

25

4817

483.5

54.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.056 ± 0.027

1.37 ± 0.011

5.357 ± 0.022

6.439 ± 0.034

3.765 ± 0.019

5.748 ± 0.025

2.338 ± 0.012

6.145 ± 0.023

5.963 ± 0.029

9.105 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.01

4.722 ± 0.017

5.403 ± 0.027

4.092 ± 0.019

5.817 ± 0.025

9.166 ± 0.032

5.93 ± 0.018

5.476 ± 0.017

1.216 ± 0.012

2.791 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski