Amino acid dipepetide frequency for Blumeria graminis f. sp. hordei (strain DH14) (Barley powdery mildew) (Oidium monilioides f. sp. hordei)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.225AlaAla: 6.225 ± 0.07
1.003AlaCys: 1.003 ± 0.021
3.188AlaAsp: 3.188 ± 0.034
4.432AlaGlu: 4.432 ± 0.046
2.506AlaPhe: 2.506 ± 0.032
4.089AlaGly: 4.089 ± 0.052
1.501AlaHis: 1.501 ± 0.024
4.363AlaIle: 4.363 ± 0.043
4.108AlaLys: 4.108 ± 0.04
6.707AlaLeu: 6.707 ± 0.052
1.523AlaMet: 1.523 ± 0.022
2.825AlaAsn: 2.825 ± 0.033
3.574AlaPro: 3.574 ± 0.046
2.935AlaGln: 2.935 ± 0.036
4.124AlaArg: 4.124 ± 0.042
6.252AlaSer: 6.252 ± 0.047
4.534AlaThr: 4.534 ± 0.043
3.984AlaVal: 3.984 ± 0.043
0.866AlaTrp: 0.866 ± 0.018
1.823AlaTyr: 1.823 ± 0.026
0.0AlaXaa: 0.0 ± 0.0
Cys
0.896CysAla: 0.896 ± 0.018
0.219CysCys: 0.219 ± 0.009
0.739CysAsp: 0.739 ± 0.015
0.764CysGlu: 0.764 ± 0.017
0.569CysPhe: 0.569 ± 0.014
0.964CysGly: 0.964 ± 0.018
0.392CysHis: 0.392 ± 0.011
0.812CysIle: 0.812 ± 0.015
0.709CysLys: 0.709 ± 0.018
1.416CysLeu: 1.416 ± 0.021
0.278CysMet: 0.278 ± 0.01
0.581CysAsn: 0.581 ± 0.015
0.711CysPro: 0.711 ± 0.018
0.558CysGln: 0.558 ± 0.013
0.794CysArg: 0.794 ± 0.018
1.262CysSer: 1.262 ± 0.021
0.749CysThr: 0.749 ± 0.015
0.728CysVal: 0.728 ± 0.011
0.188CysTrp: 0.188 ± 0.009
0.376CysTyr: 0.376 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
3.55AspAla: 3.55 ± 0.039
0.73AspCys: 0.73 ± 0.014
3.527AspAsp: 3.527 ± 0.053
4.395AspGlu: 4.395 ± 0.059
2.167AspPhe: 2.167 ± 0.027
3.274AspGly: 3.274 ± 0.038
1.159AspHis: 1.159 ± 0.02
3.485AspIle: 3.485 ± 0.036
2.709AspLys: 2.709 ± 0.032
5.06AspLeu: 5.06 ± 0.049
1.16AspMet: 1.16 ± 0.017
2.297AspAsn: 2.297 ± 0.027
2.652AspPro: 2.652 ± 0.032
1.998AspGln: 1.998 ± 0.03
2.666AspArg: 2.666 ± 0.028
4.616AspSer: 4.616 ± 0.049
2.8AspThr: 2.8 ± 0.029
3.099AspVal: 3.099 ± 0.031
0.682AspTrp: 0.682 ± 0.016
1.544AspTyr: 1.544 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
4.751GluAla: 4.751 ± 0.052
0.763GluCys: 0.763 ± 0.016
3.937GluAsp: 3.937 ± 0.048
5.254GluGlu: 5.254 ± 0.078
2.258GluPhe: 2.258 ± 0.027
3.091GluGly: 3.091 ± 0.034
1.205GluHis: 1.205 ± 0.022
4.43GluIle: 4.43 ± 0.048
4.746GluLys: 4.746 ± 0.053
5.463GluLeu: 5.463 ± 0.052
1.483GluMet: 1.483 ± 0.025
3.613GluAsn: 3.613 ± 0.045
2.464GluPro: 2.464 ± 0.037
2.344GluGln: 2.344 ± 0.038
3.693GluArg: 3.693 ± 0.034
5.101GluSer: 5.101 ± 0.059
3.688GluThr: 3.688 ± 0.043
3.552GluVal: 3.552 ± 0.041
0.801GluTrp: 0.801 ± 0.018
1.756GluTyr: 1.756 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
2.522PheAla: 2.522 ± 0.033
0.57PheCys: 0.57 ± 0.014
2.191PheAsp: 2.191 ± 0.031
2.364PheGlu: 2.364 ± 0.027
1.528PhePhe: 1.528 ± 0.026
2.498PheGly: 2.498 ± 0.037
0.948PheHis: 0.948 ± 0.018
2.178PheIle: 2.178 ± 0.028
1.892PheLys: 1.892 ± 0.027
3.517PheLeu: 3.517 ± 0.04
0.799PheMet: 0.799 ± 0.013
1.687PheAsn: 1.687 ± 0.025
1.969PhePro: 1.969 ± 0.025
1.526PheGln: 1.526 ± 0.023
1.993PheArg: 1.993 ± 0.026
3.437PheSer: 3.437 ± 0.033
2.169PheThr: 2.169 ± 0.029
2.193PheVal: 2.193 ± 0.031
0.53PheTrp: 0.53 ± 0.016
1.141PheTyr: 1.141 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
3.676GlyAla: 3.676 ± 0.049
0.791GlyCys: 0.791 ± 0.017
2.846GlyAsp: 2.846 ± 0.034
3.217GlyGlu: 3.217 ± 0.034
2.443GlyPhe: 2.443 ± 0.031
3.948GlyGly: 3.948 ± 0.063
1.404GlyHis: 1.404 ± 0.024
3.77GlyIle: 3.77 ± 0.041
3.587GlyLys: 3.587 ± 0.037
5.297GlyLeu: 5.297 ± 0.047
1.344GlyMet: 1.344 ± 0.024
2.676GlyAsn: 2.676 ± 0.031
2.571GlyPro: 2.571 ± 0.034
2.085GlyGln: 2.085 ± 0.028
3.321GlyArg: 3.321 ± 0.038
5.051GlySer: 5.051 ± 0.048
3.382GlyThr: 3.382 ± 0.036
3.387GlyVal: 3.387 ± 0.034
0.873GlyTrp: 0.873 ± 0.019
1.806GlyTyr: 1.806 ± 0.029
0.0GlyXaa: 0.0 ± 0.0
His
1.481HisAla: 1.481 ± 0.023
0.331HisCys: 0.331 ± 0.011
1.239HisAsp: 1.239 ± 0.021
1.464HisGlu: 1.464 ± 0.023
0.904HisPhe: 0.904 ± 0.016
1.499HisGly: 1.499 ± 0.023
0.732HisHis: 0.732 ± 0.02
1.438HisIle: 1.438 ± 0.022
1.133HisLys: 1.133 ± 0.02
2.236HisLeu: 2.236 ± 0.027
0.448HisMet: 0.448 ± 0.012
1.039HisAsn: 1.039 ± 0.018
1.405HisPro: 1.405 ± 0.025
1.132HisGln: 1.132 ± 0.021
1.451HisArg: 1.451 ± 0.023
2.003HisSer: 2.003 ± 0.028
1.24HisThr: 1.24 ± 0.022
1.267HisVal: 1.267 ± 0.022
0.264HisTrp: 0.264 ± 0.009
0.67HisTyr: 0.67 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
4.165IleAla: 4.165 ± 0.04
0.967IleCys: 0.967 ± 0.019
3.551IleAsp: 3.551 ± 0.034
3.879IleGlu: 3.879 ± 0.039
2.619IlePhe: 2.619 ± 0.036
3.333IleGly: 3.333 ± 0.042
1.41IleHis: 1.41 ± 0.018
3.748IleIle: 3.748 ± 0.044
3.427IleLys: 3.427 ± 0.038
5.738IleLeu: 5.738 ± 0.056
1.259IleMet: 1.259 ± 0.021
2.823IleAsn: 2.823 ± 0.033
3.473IlePro: 3.473 ± 0.039
2.418IleGln: 2.418 ± 0.033
3.273IleArg: 3.273 ± 0.033
6.066IleSer: 6.066 ± 0.05
3.438IleThr: 3.438 ± 0.037
3.311IleVal: 3.311 ± 0.039
0.759IleTrp: 0.759 ± 0.019
1.794IleTyr: 1.794 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.274LysAla: 4.274 ± 0.052
0.801LysCys: 0.801 ± 0.017
3.167LysAsp: 3.167 ± 0.04
3.936LysGlu: 3.936 ± 0.055
2.229LysPhe: 2.229 ± 0.031
2.885LysGly: 2.885 ± 0.035
1.214LysHis: 1.214 ± 0.021
4.007LysIle: 4.007 ± 0.04
4.451LysLys: 4.451 ± 0.05
5.284LysLeu: 5.284 ± 0.047
1.255LysMet: 1.255 ± 0.021
3.057LysAsn: 3.057 ± 0.035
2.864LysPro: 2.864 ± 0.03
2.117LysGln: 2.117 ± 0.026
3.722LysArg: 3.722 ± 0.038
5.22LysSer: 5.22 ± 0.049
3.386LysThr: 3.386 ± 0.031
3.299LysVal: 3.299 ± 0.034
0.681LysTrp: 0.681 ± 0.017
1.785LysTyr: 1.785 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
6.93LeuAla: 6.93 ± 0.06
1.283LeuCys: 1.283 ± 0.023
5.122LeuAsp: 5.122 ± 0.045
6.011LeuGlu: 6.011 ± 0.057
3.346LeuPhe: 3.346 ± 0.042
5.223LeuGly: 5.223 ± 0.043
2.23LeuHis: 2.23 ± 0.027
4.931LeuIle: 4.931 ± 0.05
5.223LeuLys: 5.223 ± 0.044
8.278LeuLeu: 8.278 ± 0.084
1.808LeuMet: 1.808 ± 0.028
4.001LeuAsn: 4.001 ± 0.04
5.244LeuPro: 5.244 ± 0.049
4.093LeuGln: 4.093 ± 0.045
5.59LeuArg: 5.59 ± 0.051
8.257LeuSer: 8.257 ± 0.057
5.083LeuThr: 5.083 ± 0.046
4.981LeuVal: 4.981 ± 0.051
1.034LeuTrp: 1.034 ± 0.021
2.378LeuTyr: 2.378 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
1.851MetAla: 1.851 ± 0.027
0.236MetCys: 0.236 ± 0.009
1.085MetAsp: 1.085 ± 0.019
1.353MetGlu: 1.353 ± 0.021
0.659MetPhe: 0.659 ± 0.015
1.289MetGly: 1.289 ± 0.022
0.423MetHis: 0.423 ± 0.011
1.254MetIle: 1.254 ± 0.023
1.324MetLys: 1.324 ± 0.021
1.692MetLeu: 1.692 ± 0.025
0.536MetMet: 0.536 ± 0.014
1.012MetAsn: 1.012 ± 0.018
1.092MetPro: 1.092 ± 0.021
0.837MetGln: 0.837 ± 0.018
1.228MetArg: 1.228 ± 0.018
1.947MetSer: 1.947 ± 0.029
1.288MetThr: 1.288 ± 0.02
1.163MetVal: 1.163 ± 0.02
0.224MetTrp: 0.224 ± 0.008
0.504MetTyr: 0.504 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.859AsnAla: 2.859 ± 0.032
0.706AsnCys: 0.706 ± 0.016
2.42AsnAsp: 2.42 ± 0.03
2.859AsnGlu: 2.859 ± 0.037
2.023AsnPhe: 2.023 ± 0.027
2.773AsnGly: 2.773 ± 0.036
1.17AsnHis: 1.17 ± 0.02
3.128AsnIle: 3.128 ± 0.032
2.455AsnLys: 2.455 ± 0.031
4.636AsnLeu: 4.636 ± 0.04
0.945AsnMet: 0.945 ± 0.016
2.341AsnAsn: 2.341 ± 0.033
2.724AsnPro: 2.724 ± 0.034
1.962AsnGln: 1.962 ± 0.023
2.37AsnArg: 2.37 ± 0.029
4.555AsnSer: 4.555 ± 0.049
2.773AsnThr: 2.773 ± 0.028
2.513AsnVal: 2.513 ± 0.031
0.548AsnTrp: 0.548 ± 0.014
1.454AsnTyr: 1.454 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
3.673ProAla: 3.673 ± 0.046
0.595ProCys: 0.595 ± 0.014
2.649ProAsp: 2.649 ± 0.03
3.491ProGlu: 3.491 ± 0.041
1.802ProPhe: 1.802 ± 0.025
2.934ProGly: 2.934 ± 0.037
1.302ProHis: 1.302 ± 0.021
3.103ProIle: 3.103 ± 0.035
3.133ProLys: 3.133 ± 0.036
4.557ProLeu: 4.557 ± 0.037
0.95ProMet: 0.95 ± 0.019
2.547ProAsn: 2.547 ± 0.032
3.961ProPro: 3.961 ± 0.069
2.249ProGln: 2.249 ± 0.031
2.941ProArg: 2.941 ± 0.036
5.55ProSer: 5.55 ± 0.051
3.695ProThr: 3.695 ± 0.039
2.979ProVal: 2.979 ± 0.035
0.534ProTrp: 0.534 ± 0.015
1.382ProTyr: 1.382 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
2.974GlnAla: 2.974 ± 0.035
0.505GlnCys: 0.505 ± 0.015
1.999GlnAsp: 1.999 ± 0.028
2.499GlnGlu: 2.499 ± 0.033
1.395GlnPhe: 1.395 ± 0.021
2.064GlnGly: 2.064 ± 0.027
0.952GlnHis: 0.952 ± 0.019
2.653GlnIle: 2.653 ± 0.032
2.731GlnLys: 2.731 ± 0.035
3.529GlnLeu: 3.529 ± 0.042
0.839GlnMet: 0.839 ± 0.017
2.25GlnAsn: 2.25 ± 0.03
2.151GlnPro: 2.151 ± 0.03
2.146GlnGln: 2.146 ± 0.054
2.504GlnArg: 2.504 ± 0.033
3.532GlnSer: 3.532 ± 0.039
2.445GlnThr: 2.445 ± 0.026
2.168GlnVal: 2.168 ± 0.027
0.438GlnTrp: 0.438 ± 0.012
1.147GlnTyr: 1.147 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
4.038ArgAla: 4.038 ± 0.038
0.807ArgCys: 0.807 ± 0.018
3.037ArgAsp: 3.037 ± 0.034
3.743ArgGlu: 3.743 ± 0.043
2.012ArgPhe: 2.012 ± 0.027
3.219ArgGly: 3.219 ± 0.039
1.414ArgHis: 1.414 ± 0.021
3.39ArgIle: 3.39 ± 0.033
4.048ArgLys: 4.048 ± 0.041
5.297ArgLeu: 5.297 ± 0.049
1.232ArgMet: 1.232 ± 0.022
2.831ArgAsn: 2.831 ± 0.034
3.018ArgPro: 3.018 ± 0.041
2.569ArgGln: 2.569 ± 0.031
4.327ArgArg: 4.327 ± 0.052
4.897ArgSer: 4.897 ± 0.047
3.019ArgThr: 3.019 ± 0.034
2.989ArgVal: 2.989 ± 0.031
0.727ArgTrp: 0.727 ± 0.017
1.555ArgTyr: 1.555 ± 0.024
0.0ArgXaa: 0.0 ± 0.0
Ser
5.688SerAla: 5.688 ± 0.045
1.184SerCys: 1.184 ± 0.021
4.565SerAsp: 4.565 ± 0.052
5.147SerGlu: 5.147 ± 0.047
3.276SerPhe: 3.276 ± 0.038
5.163SerGly: 5.163 ± 0.039
2.328SerHis: 2.328 ± 0.03
5.516SerIle: 5.516 ± 0.049
5.311SerLys: 5.311 ± 0.048
8.28SerLeu: 8.28 ± 0.069
1.856SerMet: 1.856 ± 0.023
4.598SerAsn: 4.598 ± 0.046
5.316SerPro: 5.316 ± 0.057
3.847SerGln: 3.847 ± 0.04
5.457SerArg: 5.457 ± 0.053
10.072SerSer: 10.072 ± 0.086
6.096SerThr: 6.096 ± 0.058
4.525SerVal: 4.525 ± 0.037
1.048SerTrp: 1.048 ± 0.019
2.382SerTyr: 2.382 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
4.258ThrAla: 4.258 ± 0.039
0.818ThrCys: 0.818 ± 0.019
2.76ThrAsp: 2.76 ± 0.034
3.328ThrGlu: 3.328 ± 0.038
2.124ThrPhe: 2.124 ± 0.023
3.585ThrGly: 3.585 ± 0.038
1.346ThrHis: 1.346 ± 0.022
3.668ThrIle: 3.668 ± 0.035
3.374ThrLys: 3.374 ± 0.037
5.263ThrLeu: 5.263 ± 0.045
1.157ThrMet: 1.157 ± 0.02
2.755ThrAsn: 2.755 ± 0.033
3.846ThrPro: 3.846 ± 0.044
2.248ThrGln: 2.248 ± 0.027
3.219ThrArg: 3.219 ± 0.033
6.019ThrSer: 6.019 ± 0.053
4.073ThrThr: 4.073 ± 0.04
3.223ThrVal: 3.223 ± 0.038
0.701ThrTrp: 0.701 ± 0.015
1.538ThrTyr: 1.538 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
4.1ValAla: 4.1 ± 0.038
0.745ValCys: 0.745 ± 0.016
3.236ValAsp: 3.236 ± 0.035
3.833ValGlu: 3.833 ± 0.04
2.101ValPhe: 2.101 ± 0.026
3.194ValGly: 3.194 ± 0.041
1.195ValHis: 1.195 ± 0.018
3.234ValIle: 3.234 ± 0.036
3.153ValLys: 3.153 ± 0.038
4.823ValLeu: 4.823 ± 0.042
1.217ValMet: 1.217 ± 0.02
2.355ValAsn: 2.355 ± 0.027
3.05ValPro: 3.05 ± 0.036
2.144ValGln: 2.144 ± 0.026
3.086ValArg: 3.086 ± 0.032
4.542ValSer: 4.542 ± 0.04
3.221ValThr: 3.221 ± 0.042
3.263ValVal: 3.263 ± 0.044
0.679ValTrp: 0.679 ± 0.017
1.591ValTyr: 1.591 ± 0.024
0.0ValXaa: 0.0 ± 0.0
Trp
0.864TrpAla: 0.864 ± 0.019
0.172TrpCys: 0.172 ± 0.007
0.73TrpAsp: 0.73 ± 0.019
0.731TrpGlu: 0.731 ± 0.017
0.423TrpPhe: 0.423 ± 0.013
0.668TrpGly: 0.668 ± 0.017
0.295TrpHis: 0.295 ± 0.01
0.737TrpIle: 0.737 ± 0.018
0.812TrpLys: 0.812 ± 0.016
1.127TrpLeu: 1.127 ± 0.02
0.304TrpMet: 0.304 ± 0.01
0.632TrpAsn: 0.632 ± 0.012
0.523TrpPro: 0.523 ± 0.014
0.47TrpGln: 0.47 ± 0.013
0.782TrpArg: 0.782 ± 0.015
0.924TrpSer: 0.924 ± 0.02
0.695TrpThr: 0.695 ± 0.014
0.671TrpVal: 0.671 ± 0.016
0.223TrpTrp: 0.223 ± 0.009
0.378TrpTyr: 0.378 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.787TyrAla: 1.787 ± 0.029
0.477TyrCys: 0.477 ± 0.012
1.581TyrAsp: 1.581 ± 0.024
1.691TyrGlu: 1.691 ± 0.026
1.268TyrPhe: 1.268 ± 0.022
1.785TyrGly: 1.785 ± 0.026
0.755TyrHis: 0.755 ± 0.015
1.608TyrIle: 1.608 ± 0.025
1.307TyrLys: 1.307 ± 0.02
2.847TyrLeu: 2.847 ± 0.034
0.604TyrMet: 0.604 ± 0.014
1.296TyrAsn: 1.296 ± 0.021
1.421TyrPro: 1.421 ± 0.024
1.243TyrGln: 1.243 ± 0.023
1.628TyrArg: 1.628 ± 0.022
2.35TyrSer: 2.35 ± 0.03
1.531TyrThr: 1.531 ± 0.023
1.465TyrVal: 1.465 ± 0.022
0.358TyrTrp: 0.358 ± 0.013
0.911TyrTyr: 0.911 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6486 proteins (3136009 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski